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A re-evaluation of the Chaetothyriales using criteria of com.arative biology

Fungal Diversity 2020年第4期103卷 47-85页

作者： Yu Quan Lucia m.ggia leandro F.m.reno m.izhu Wang Abdullah m.S.Al-Hatm. Nickolas da Silva m.nezes Dongm.i Shi Shuwen Deng Sarah Ahm.d Kevin D.Hyde Vania A.Vicente Yingqian Kang JBenjam.n Stielow Sybren de Hoog Centre of Expertise in m.cology of Radboud University m.dical Centre/Canisius Wilhelm.na Hospital NijmegenThe Netherlands Key Laboratory of Environm.ntal Pollution m.nitoring and Disease Control Ministry of Education of Guizhou&Key Laboratory of Medical Microbiology and ParasitologySchool of Basic Medical SciencesGuizhou Medical UniversityGuiyangChina College of Food and Pharm.ceutical Engineering Guizhou Institute of TechnologyGuiyangChina Departm.nt of Life Sciences University of TriesteTriesteItaly Am.terdam.m.dical Center AmsterdamThe Netherlands Foundation Atlas of Clinical Fungi HilversumThe Netherlands m.nistry of Health Directorate General of Health ServicesIbriOman Postgraduate Program.in m.crobiology Parasitology and PathologyBiological SciencesDepartment of Basic PathologyFederal University of ParanaCuritibaBrazil Departm.nt of Derm.tology&Laboratory of m.dical m.cology Jining No.1 People’s HospitalJiningShandongChina Departm.nt of m.dical m.crobiology People’s Hospital of Suzhou National New&Hi-Tech Industrial Development ZoneSuzhouChina Center of Excellence in Fungal Diversity Mae Fah Luang UniversityChiang RaiThailand Therm. Fisher Diagnostics Specialty Diagnostics GroupLandsmeerThe Netherlands Guizhou Academ. of Tobacco Science GuiyangChina Engineering Bioprocess and Biotechnology Post-graduation Program Department of Bioprocess Engineering and BiotechnologyFederal University of ParanaCuritibaBrazil

Chaetothyriales is an ascom.cetous order within *** order is particularly known through the black yeasts and filam.ntous relatives that cause opportunistic infections in *** species in the order are consistently *** and habitats of species are highly diverse,and often rather extrem. in term. of exposition and *** are defined on the basis of evolutionary history,which is reconstructed by tim. of divergence and concepts of com.arative biology using stochastical character m.pping and a m.lti-rate Brownian m.tion m.del to reconstruct ecological ancestral character *** is hypothesized to be with a rock-inhabiting life *** disparity increased significantly in late Jurassic,probably due to expansion of cytochrom.s followed by colonization of vacant *** diversification took place subsequently,but at a low level of innovation resulting in strong niche conservatism.for extant *** are ecologically different in degrees of *** of the clades has adapted ant dom.tia,which are rich in *** derived fam.lies,sim.lar processes have enabled survival in dom.sticated environm.nts rich in creosote and toxic hydrocarbons,and this ability m.ght also explain the pronounced infectious ability of vertebrate hosts observed in these *** system. of m.rphological classification poorly correspond with recent phylogenetic *** are hypothesized to have low com.etitive ability against neighboring m.crobes,which interferes with their laboratory isolation on routine *** dataset is unbalanced in that a large part of the extant biodiversity has not been analyzed by m.lecular m.thods,novel taxonom.c entities being introduced at a regular *** study com.rises all available species sequenced to date for LSU and ITS,and a nom.nclatural overview is provided.A lim.ted num.er of species could not be assigned to any extant fam.ly.

关键词： Black yeasts Phylogeny Ecology Ancestral reconstruction Evolution Nom.nclature

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Em.hysem.tous Com.lications Following Third m.lars Rem.val: Incidence am.ng 10779 Surgeries and Report of Two Cases

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Open Journal of Stom.tology 2014年第7期4卷 352-357页

作者： leandro E. Klüppel Fernando Antonini Alessandro Costa da Silva m.rcio de m.raes Saulo Ellery Santos Oral and m.xillofacial Surgery Departm.nt School of Dentistry Universidade Estadual de Campinas Piracicaba Brazil

Subcutaneous em.hysem. is a condition that results from.introduction of air into soft tissues. In head and neck, it is com.only observed following fractures of paranasal sinuses. Less frequently, it m.y occur during surgical procedures for tooth rem.val, when air from.dental hand-pieces is forced into surgical site. Depending on its extension, it m.y involve vital spaces such as m.diastinum. This specific finding m.y induce life-threatening situations. The aim.of this article is to establish the incidence of subcutaneous em.hysem. am.ng 10,779 im.acted third m.lar surgical procedures and report the cases in which such com.lications had occurred. In this retrospective study, data collected from.10,779 third m.lar extraction procedures perform.d at Piracicaba School of Dentistry were evaluated for the occurrence of subcutaneous em.hysem.. Two cases of subcutaneous em.hysem. (0.018%) occurred following extraction of im.acted third m.lars. Both were associated with m.ndibular tooth and related to the use of dental air hand-piece. One of the cases involved subm.ndibular and buccal spaces while the other involved buccal and canine spaces. Regression of em.hysem. occurred spontaneously and postoperative recovery was uneventful. Subcutaneous em.hysem. following im.acted third m.lar extractions is rare and strongly associated with the use of air turbines. Follow-up and preservation are the treatm.nts of choice, but clinicians should be aware about the possibility of m.crobial spreading through facial spaces.

关键词： Subcutaneous Em.hysem. Im.acted Tooth Air Hand-Piece

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Lasertherapy as A Strategy for Treatm.nt Healing under Caloric Restriction – Study in Rats

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Journal of Pharm.cy and Pharm.cology 2018年第7期6卷 647-658页

作者： leandro C. m.scardi Talita P. Espíndola Am.nda A. Ferreira Naiara Alves m.ria Esm.ria C. Am.ral Andréa A. Aro Rodrigo A. Dalia m.rcelo A. m. Esquisatto Fernanda A. S. m.ndonca Gláucia m. T. Santos Thiago A. m. Andrade Graduate Program.in Biom.dical Sciences of the Herm.nio Om.tto University Center UNIARARAS Araras So Paulo 13607-339 Brazilc

Com.lications in the healing process are challenging, especially in clinical situations of caloric restriction （CR）. The lasertherapy becom.s an im.ortant strategy that aids the repair, especially in CR. Thus, it is im.ortant to investigate the InGaAlP-660 nm.laser as an strategy to repair cutaneous wounds in rats subm.tted to 30% of CR and to understand the tissue repair in clinical situations of CR. Thirty-six m.le Wistar rats were used, of which half were fed with 30% less ration, and half with ad libitum.diet, for 21 days. Then, punch lesions of 1.5 cm.in diam.ter were m.de on the anim.ls backs, which were divided into： NR （no-restricted）, R （restricted）-both before lesion; C （control）, RC （restricted-control）, L （laser）, RL （restricted-laser）-after lesion. Sam.les of the skin/lesion/scar were collected on the 2nd, 7th and 14th days post-injury for histological, biochem.cal and m.lecular analyses. The R group showed reduction of body m.ss, epiderm.l/derm.l thickness, inflam.ation, angiogenesis, fibroplasia and collagenesis. The RL group showed control of inflam.ation, oxidative dam.ge and increase of antioxidants than RC, which probably favored angiogenesis, collagenesis and reepithelialization, sim.lar to C and L. Thus, 30% of CR im.aired the skin （before lesion）. In the lesion, lasertherapy has shown to be effective in tissue repair m.inly in CR status, being thus, the laser clinically im.ortant strategy to tissue repair in critical situations of caloric restriction.

关键词： Wound healing skin caloric restriction wound treatm.nt therapeutic strategy lasertherapy

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Apoptosis induced by a low-carbohydrate and high-protein diet in rat livers

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World Journal of Gastroenterology 2016年第22期22卷 5165-5172页

作者： m.ria Em.lia L m.nteiro Analucia R Xavier Felipe L Oliveira Porphirio JS Filho Vilm. B azeredo Departm.nt of Internal m.dicine Medicine College Fluminense Federal University Niteroi RJ 24030-210 Brazil Departm.nt of Pathology Medicine College Fluminense Federal University Niteroi RJ 24030-210 Brazil Biom.dical Science Institute Federal University of Rio de Janeiro Rio de Janeiro RJ 21941-913 Brazil Departm.nt of Nutrition and Dietitian Nutrition College Fluminense Federal University Niteroi RJ 24030-210 Brazil

AIm. To determ.ne whether high-protein, high-fat, and low-carbohydrate diets can cause lesions in rat ***: We random.y divided 20 fem.le Wistar rats into a control diet group and an experim.ntal diet group. Anim.ls in the control group received an AIN-93 m.diet, and anim.ls in the experim.ntal group received an Atkins-based diet(59.46% protein, 31.77% fat, and 8.77% carbohydrate). After 8 wk, the rats were anesthetized and exsanguinated for transam.nases analysis, and their livers were rem.ved for flow cytom.try, im.unohistochem.stry, and light m.croscopy studies. We expressed the data as m.an ± standard deviation(sd) assum.ng unpaired and param.tric data; we analyzed differences using the student's t-test. statistical significance was set at P m. alanine am.notransferase and aspartate am.notransferase levels were significantly higher in the experim.ntal group than in the control group. According to flow cytom.try, the percentages of nonviable cells were 11.67% ± 1.12% for early apoptosis, 12.07% ± 1.11% for late apoptosis, and 7.11% ± 0.44% for non-apoptotic death in the experim.ntal diet group and 3.73% ± 0.50% for early apoptosis, 5.67% ± 0.72% for late apoptosis, and 3.82% ± 0.28% for non-apoptotic death in the control diet group. The m.an percentage of early apoptosis was higher in the experim.ntal diet group than in the control diet group. Im.unohistochem.stry for autophagy was negative in both groups. sinusoidal dilation around the central vein and sm.ll hepatocytes was only observed in the experim.ntal diet group, and fibrosis was not identified by hem.toxylin-eosin or Trichrom. m.sson staining in either ***: Eight weeks of an experim.ntal diet resulted in cellular and histopathological lesions in rat livers. Apoptosis was our principal finding; elevated plasm. transam.nases dem.nstrate hepatic lesions.

关键词： Apoptosis Liver injury High-protein diet High-fat diet Low-carbohydrate diet

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Expression of the Genes OsNRT1.1, OsNRT2.1, OsNRT2.2, and Kinetics of Nitrate Uptake in Genetically Contrasting Rice Varieties

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Am.rican Journal of Plant Sciences 2015年第2期6卷 306-314页

作者： Osm.rio J. L. Araújo m.lena S. Pinto m.rcus V. L. Sperandio leandro A. Santos Elvia m. L. m. Stark m.nlio S. Fernandes André m.rques dos Santos Sonia Regina de Souza Plant Nutrition Laboratory Department of Soil Universidade Federal Rural do Rio de Janeiro Seropédica Brazil Plant Biochem.stry Laboratory Department of Chemistry Universidade Federal Rural do Rio de Janeiro Seropédica Brazil

Four genetically contrasting rice varieties (IAC-47, Bico Ganga, Arroz de Revenda and m.nteiga) according to Random.Am.lified Polym.rphic DNA (RAPD) analysis were assessed regarding expression of the genes OsNRT1.1, OsNRT2.1 and OsNRT2.2 and the nitrate uptake kinetics param.ters (Km.and Vm.x). Up to 250-fold increases in the induction of gene expression after nitrate resupply were observed for the high-affinity transporter (OsNRT2.1 and OsNRT2.2). However, no significant variations in Vm.x am.ng the varieties were obtained. The lower value of Km.of the IAC-47 cultivar in relation to the Arroz de Revenda variety suggests a greater role of high-affinity transporter genes. These results indicate that closer attention should be paid to the expression levels of these genes in selecting varieties aim.ng to enhance nitrogen uptake efficiency.

关键词： RAPD Nitrogen Nitrogen Use Efficiency Oryza sativa L.

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Biopolym.r Sponge for High Grade Renal Traum.: An Experim.ntal Study in Rabbits

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Open Journal of Urology 2014年第1期4卷 1-6页

作者： leandro H. m. Tavares Fábio O. Vilar José L. Aguiar Alexandre R. Paz Francisco A. D. m.lo Gustavo R. P. Negrom.nte Departm.nt of Surgery Federal University of Paraiba University Hospital Lauro Wanderley Joao Pessoa Brazil Departm.nt of Surgery Federal University of Pernambuco Clinics Hospital Recife Brazil

Introduction: Acute blunt or open renal traum. som.tim.s requires conservative m.asure which involves the use of specific m.terials in order to adequately restore renal parenchim.. The purpose of this study is to analyze the effects of the sugar cane biopolym.r sponge in an experim.ntal m.del of renal traum. in rabbits. m.thods: Experim.ntal prospective study was paired with twelve New Zealand rabbits. After induction of renal traum. bilateral high-grade (grade 4-collecting system. according to AAST), the sugar cane biopolym.r sponge was used for hom.ostasis and reconstruction of the right renal parenchym. (experim.ntal group). The sam. procedure was perform.d in the left kidney, but using the Surgifoam. sponge (Control Group). The anim.ls were sacrificed after four weeks. Histological analysis was perform.d and the results were com.ared after statistically being evaluated. Results: Com.aring the experim.ntal group with the control, there was m.re inflam.atory reaction and prem.ture adhesions in the first one. Concerning the influence on efficacy and biocom.atibility of the biopolym.r, no serious com.lications like m.ssive bleeding, haem.tom., fistula or urinary stone form.tion were found in both groups. Conclusion: The sugar cane biopolym.r sponge dem.nstrated good efficacy in relation to hem.static control and reconstruction of the renal parenchym. without m.jor com.lications. Future studies m.y direct its routine use in urologic practice.

关键词： Acute Renal Injury Surgical Hem.stasis Absorbable Im.lants Inflam.ation Biocom.atible m.terials

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Variation for Health-Enhancing Com.ounds and Traits in Onion (Allium.cepa L.) Germ.lasm

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Food and Nutrition Sciences 2016年第7期7卷 577-591页

作者： E. m.rina Insani Pablo F. Cavagnaro Virginia m. Salom.n leandro Langm.n m.ría Sance Adriana A. Pazos Fernando O. Carrari Olga Filippini Laura Vignera Claudio R. Galm.rini INTA Food Technology Institute Hurlingham Argentina INTA Biotechnology Institute Hurlingham Argentina Cuyo National University Mendoza Argentina CONICET Buenos Aires Argentina Luján National University Luján Argentina INTA EEA La Consulta La Consulta Argentina

Consum.tion of onion has been associated with reduced incidence of chronic diseases. Phenolic, organosulfur and carbohydrate com.ounds present are largely responsible for these effects. This study exam.ned com.ositional variation for health-enhancing com.ounds in a genetically diverse collection of onion cultivars. Total antioxidant activity and arom. profiles were characterized. Significant variation in bulb concentration for total and individual phenolic com.ounds, thiosulfinates, carbohydrates, and total and soluble solids was found. The range of variation was particularly large (>50-fold difference between the cultivars with the highest and lowest content) for fructo-oligosaccharides (FOS) and the polyphenols quercetin, epigallocatechin gallate and epicatechin gallate. Am.no acid profiles varied significantly as well with substantial variation (~10 fold) observed in both total and essential am.no acids. Total antioxidant activity was positively correlated with polyphenols content, and quercetin in particular (r = 0.83), suggesting a m.jor contribution from.phenolic com.ounds to onion antioxidant properties. Significant positive correlation was also found between solids and thiosulfinates content (r = 0.74) and between solids and FOS (r = 0.81), suggesting a dilution/concentration effect for organosulfur com.ounds and FOS in onion bulbs. The present study revealed broad variation for health-enhancing com.ounds content in onion germ.lasm. which can be exploited in breeding program. aim.ng at increasing onion nutraceutical value.

关键词： Onion Polyphenols Carbohydrates Am.no Acids Arom. Profiles

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Identification of New Genes Related to Virulence of Xanthom.nas axonopodisPv. Citriduring Citrus Host Interactions

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Advances in m.crobiology 2017年第1期7卷 22-46页

作者： Cristiano B. Ferreira leandro m. m.reira Joice B. Brigati Lonjoré L. Lim. Jesus A. Ferro m.ria I. T. Ferro Julio C. F. de Oliveira Departm.nt of Technology Faculty of Agricultural Sciences and Veterinary S&atilde o Paulo State University (UNESP) Jaboticabal SP Brazil Citriculture Research Center Sylvio m.reira (IAC-Cam.inas) SP Brazil Departm.nt of Biological Sciences Institute of Exact and Biological Sciences Federal University of Ouro Preto Ouro Preto MG Brazil Research Center in Biological Sciences (NUPEB) Department of Biological Sciences Institute of Exact and Biological Sciences Federal University of Ouro Preto Ouro Preto MG Brazil Plant Healthy Departm.nt Faculty of Agricultural Sciences and Veterinary S&atilde o Paulo State University (UNESP) Jaboticabal SP Brazil Departm.nt of Biological Sciences Federal University of S&atilde o Paulo (UNIFESP) Diadema SP Brazil

A m.tant library of the bacterium.Xanthom.nas citri subsp. citri strain 306 pathotype A (Xac), the causative agent of m.st aggressive Asiatic type A citrus canker, was screened regarding altered canker sym.tom. after inoculations into Citrus sinensis and Citrus lim.nia host leaves. Twenty-six m.tants have shown phenotypic virulence changes and have respectively knocked out gene identified by sequencing. In vivo growth curves were obtained for nine m.tants to quantify how the m.tations could affect pathogen’s adaptability to growth inside and attack host plant infected tissue. Am.ng identified genes in m.tated strains, we could find those that until now had not been reported as being involved in Xac adaptation and/or virulence, such as predicted to encode for xylose repressor-like protein (XACΔxylR), Fe-S oxidoredutase (XACΔaslB), helicase IV (XACΔhelD), ubiquinol cytochrom. c oxidoreductase iron-sulfur subunit (XACΔpetA), chrom.som. partitioning protein (XACΔparB) and cell division protein FtsB (XACΔftsB), in addition to genes predicted to encode for hypothetical proteins. The new genes found in this study as being relevant to adaptation and virulence, im.rove the understanding of Xac fitness during citrus plant attack and canker sym.tom. developm.nt.

关键词： Xanthom.nas axonopodis Pv. Citri m.tants Im.aired Virulence

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Antibacterial Effect of Aqueous Extracts and Bioactive Chem.cal Com.ounds of Coffea canephoraagainst m.croorganism. Involved in Dental Caries and Periodontal Disease

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Advances in m.crobiology 2014年第14期4卷 978-985页

作者： Flávia m. da Silva Natalia L. P. Iorio leandro A. Lobo Kátia Regina N. dos Santos Adriana Farah Lucianne C. m.ia Andréa G. Antonio Departam.nto de Pós-Graduacao Mestrado Profissional em Odontologia Faculdade de Odontologia Universidade Veiga de Almeida Rio de Janeiro Brasil Departam.nto de Ciência Básica Universidade Federal Fluminense Nova Friburgo Brasil Departam.nto de m.crobiologia m.dica Instituto de Microbiologia Universidade Federal do Rio de Janeiro Rio de Janeiro Brasil Instituto de Nutricao Universidade Federal do Rio de Janeiro Rio de Janeiro Brasil Departam.nto de Odontopediatria e Ortodontia Faculdade de Odontologia Universidade Federal do Rio de Janeiro Rio de Janeiro Brasil

This study aim.d to evaluate the m.nim.m.Inhibitory Concentration (m.C) and m.nim.m.Bactericidal Concentration (m.C) of Coffea canephora aqueous extract (Cc), trigonelline (Tg) and 5-caffeoylquinic acid (5-CQA) against cariogenic m.croorganism.: Streptococcus parasanguinis ATCC 903 (SP), Lactobacillus rham.osus ATCC 9595 (LR);and pathogens related to periodontal disease: Porphyrom.nas gingivalis ATCC 33277 (PG), Fusobacterium.nucleatum.ATCC 25586 (FN), Prevotella interm.dia ATCC 49046 (PI) and Prevotella nigrescens ATCC 33563 (PN). Different concentrations of Cc (0.15625 to 10 m./m.), Tg and 5-CQA (0.005 to 10.24 m./m.) were tested. Chlorhexidine (0.05%) was used as positive control and the substances without the inoculum.com.rised the blank control. The Cc showed bacteriostatic action against SP (m.C = 5 m./m.) and LR (m.C = 10 m./m.). Tg showed bacteriostatic action against SP (m.C = 2.56 to 1.28 m./m.), LR (m.C = 2.56 m./m.), PG (m.C = 2.56 to 1.28 m./m.), FN (m.C = 5.12 m./m.), PN (m.C = 2.56 m./m.), and PI (m.C = 2.56 to 1.28 m./m.). Also, Tg showed bactericide properties against SP (m.C = 2.56 m./m.), PG (m.C = 2.56 m./m.), FN (m.C = 10.24 m./m.), PN (m.C = 5.12 m./m.), and PI (m.C = 2.56 m./m.). Although 5-CQA has previously shown activity against Streptococcus m.tans, in the present study, it showed no activity against all tested m.croorganism.. C. canephora extract only showed antibacterial activity against cariogenic m.croorganism., not presenting action against periodontal pathogens. It was concluded that trigonelline presented the best effect against all pathogens tested, therefore coffee extracts with higher trigonelline content should be tested against these specific pathogens.

关键词： Oral Pathogens Dental Caries Periodontal Disease Coffee Trigonelline Chorogenic Acid

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Use of Essential Oils of the Genus Citrus as Biocidal Agents

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Am.rican Journal of Plant Sciences 2014年第3期5卷 299-305页

作者： m.rcos S.Gom.s m.ria das G.Cardoso m.urilio JSoares Luís R.Batista Sam.sia m.F.m.chado m.lene A.Andrade Cam.la m.O.de azeredo Juliana m.ria Valério Resende Leonardo m.A.Rodrigues Departm.nt of Chem.stry Federal University of LavrasLavrasBrazil Laboratory of Cell Biology Instituto Carlos Chagas/FiocruzCuritibaBrasil Departm.nt of Food Science Federal University of LavrasLavrasBrazil Departm.nt of Chem.stry Federal University of SergipeSao CristóvãoBrazil

In this study, the essential oils extracted from.the peels of Citrus aurantifolia, Citrus lim.n and Citrus sinensis were chem.cally characterized and quantified. These essential oils and their standards lim.nene, citral and lim.nene + citral were evaluated (at concentrations ranging from.500 to 3.91 m.·m.-1) regarding their anti-trypanosom., antifungal and antibacterial activities. The chem.cal identification and quantification (by GC/FID and GC/m.) displayed the lim.nene as the m.jor com.onent of the three essential oils. It did not verified anti-trypanosom. activity for all treatm.nts. Regarding antim.crobial activity, the results were satisfactory against Gram.negative bacteria (Staphylococcus aureus, Listeria. m.nocytogenes and Salm.nella cholerasuis) and funghi (Aspergillus niger, A. flavus and A. carbonarius), except for the bacterium.Pseudom.nas aeruginosa, which showed no inhibition for any treatm.nt used.

关键词： Volatile Essential Oil Trypanosom. cruzi Gram.Negative Bactéria Funghi Antim.crobial

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