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Timp1 Deletion Induces Anxiety-like Behavior in Mice

Neuroscience Bulletin 2024年第6期40卷 732-742页

作者： Xiaotong Wang Wei Zheng Ziyi Zhu Biyu Xing Weijie Yan Ke Zhu Lingli Xiao Chaojuan yang Mengping Wei lei yang Zi-Bing Jin Xueyun Bi Chen Zhang State Key Laboratory of Neurology and Oncology Drug Development Nanjing210000China School of Basic Medical Sciences Wenzhou Medical UniversityWenzhou325035China School of Basic Medical Sciences Beijing Key Laboratory of Neural Regeneration and RepairAdvanced Innovation Center for Human Brain ProtectionBeijing Laboratory of Oral HealthCapital Medical UniversityBeijing100069China Beijing Institute of Ophthalmology Beijing Tongren Eye CenterBeijing Tongren HospitalCapital Medical UniversityBeijing100005China Chinese Institute for Brain Research Beijing102206China Key Laboratory for Biomechanics and Mechanobiology of the Ministry of Education Beijing Advanced Innovation Center for Biomedical EngineeringSchool of Engineering MedicineBeihang UniversityBeijing100191China

The hippocampus is essential for learning and memory,but it also plays an important role in regulating emotional behavior,as hippocampal excitability and plasticity affect anxiety and *** synaptic plasticity may be regulated by tissue inhibitor of matrix metalloproteinase 1(TIMP1),a known protein inhibitor of extracellular matrix(ECM),and the expression of TIMP1 in the hippocampus can be induced by neuronal excitation and various ***,the involvement of Timp1 in fear learning,anxiety,and hippocampal synaptic function remains to be *** study of Timp1 function in vivo revealed that Timp1 knockout mice exhibit anxiety-like behavior but normal fear *** results suggested that Timp1 knockout mice showed hyperactivity in the ventral CA1 region,but the basic synaptic transmission and plasticity were normal in the Schaffer collateral *** together,our results suggest that deletion of Timp1 in vivo leads to the occurrence of anxiety behaviors,but that Timp1 is not crucial for fear learning.

关键词： Timp1 Anxiety Fear Synaptic transmission Synaptic plasticity

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Advancing CRISPR base editing technology through innovative strategies and ideas

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Science China Life Sciences 2024年

作者： Xiongwei Fan yang lei Liren Wang Xiushan Wu Dali Li The Center for Heart Development College of Life Science Hunan Normal University Shanghai Frontiers Science Research Base of Genome Editing and Cell Therapy Shanghai Key Laboratory of Regulatory Biology Institute of Biomedical Sciences and School of Life Sciences East China Normal University Guangdong Provincial Key Laboratory of Pathogenesis Targeted Prevention and Treatment of Heart Disease

The innovation of CRISPR/Cas gene editing technology has developed rapidly in recent years. It is widely used in the fields of disease animal model construction, biological breeding, disease diagnosis and screening, gene therapy, cell localization, cell lineage tracking, synthetic biology, information storage, etc. However, developing idealized editors in various fields is still a goal for future development. This article focuses on the development and innovation of non-DSB editors BE and PE in the platform-based CRISPR system. It first explains the application of ideas for improvement such as “substitution”, “combination”, “adaptation”, and “adjustment” in BE and PE development and then catalogues the ingenious inversions and leaps of thought reflected in the innovations made to CRISPR technology. It will then elaborate on the efforts currently being made to develop small editors to solve the problem of AAV overload and summarize the current application status of editors for in vivo gene modification using AAV as a delivery system. Finally, it summarizes the inspiration brought by CRISPR/Cas innovation and assesses future prospects for development of an idealized editor.

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Microvesicles derived from mesenchymal stem cells inhibit acute respiratory distress syndrome-related pulmonary fibrosis in mouse partly through hepatocyte growth factor

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World Journal of Stem Cells 2024年第8期16卷 811-823页

作者： Qi-Hong Chen Ying Zhang Xue Gu Peng-lei yang Jun Yuan Li-Na Yu Jian-Mei Chen Department of Critical Care Medicine Jiangdu People’s Hospital of YangzhouJiangdu People’s Hospital Affiliated to Yangzhou UniversityYangzhou 225200Jiangsu ProvinceChina Department of Critical Care Medicine Jiangdu People’s Hospital of YangzhouYangzhou 225200Jiangsu ProvinceChina Institute of Translational Medicine Medical CollegeYangzhou UniversityYangzhou 225000Jiangsu ProvinceChina

BACKGROUND Pulmonary fibrosis is one of the main reasons for the high mortality rate among acute respiratory distress syndrome(ARDS)*** stromal cell-derived microvesicles(MSC-MVs)have been shown to exert antifibrotic effects in lung *** To investigate the effects and mechanisms of MSC-MVs on pulmonary fibrosis in ARDS mouse *** MSC-MVs with low hepatocyte growth factor(HGF)expression(siHGF-MSC-MVs)were obtained via lentivirus transfection and used to establish the ARDS pulmonary fibrosis mouse *** intubation,respiratory mechanics-related indicators were measured via an experimental small animal lung function *** of MSC-MVs in lung tissues was investigated by near-infrared live ***,western blotting,ELISA and other methods were used to detect expression of pulmonary fibrosis-related proteins and to compare effects on pulmonary fibrosis and fibrosis-related *** The MSC-MVs gradually migrated and homed to damaged lung tissues in the ARDS model *** with MSC-MVs significantly reduced lung injury and pulmonary fibrosis ***,low expression of HGF(siHGF-MSC-MVs)significantly inhibited the effects of MSC-MVs(P<0.05).Compared with the ARDS pulmonary fibrosis group,the MSC-MVs group exhibited suppressed expression of type I collagen antigen,type III collagen antigen,and the proteins transforming growth factor-βandα-smooth muscle actin,whereas the siHGF-MVs group exhibited significantly increased expression of these *** addition,pulmonary compliance and the pressure of oxygen/oxygen inhalation ratio were significantly lower in the MSC-MVs group,and the effects of the MSC-MVs were significantly inhibited by low HGF expression(all P<0.05).CONCLUSION MSC-MVs improved lung ventilation functions and inhibited pulmonary fibrosis in ARDS mice partly via HGF mRNA transfer.

关键词： Microvesicles derived from mesenchymal stem cells Acute respiratory distress syndrome Pulmonary fibrosis Hepatocyte growth factor Mesenchymal stromal cells

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新时代教育评价类型化改革与差异化路径

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民族教育研究 2024年第3期35卷 61-68页

作者：杨磊西南大学教育学部重庆400715

新时代教育评价类型化改革的目的是建立职业教育与普通教育“双轨并行,有条件融合”的现代化教育评价体系。首先,以类型评价导向完善现代教育体系,推进教育强国建设进程;其次,纠偏社会大众对技术技能的固有偏见,加速迈进技能型社会;最后... 详细信息

新时代教育评价类型化改革的目的是建立职业教育与普通教育“双轨并行,有条件融合”的现代化教育评价体系。首先,以类型评价导向完善现代教育体系,推进教育强国建设进程;其次,纠偏社会大众对技术技能的固有偏见,加速迈进技能型社会;最后,尊重不同类型知识的习得规律,助推不同禀赋学生健康成长。然而,在教育评价类型化改革进程中却存在如下困境:一是教育评价体系改革难度与社会影响力过载的“结构性”困境;二是高考文化惯性与精英主义双重评价桎梏的文化再生产困境;三是优质教育资源稀缺导致评价筛选功能过强的资源分布困境。为保障新时代教育评价类型化改革的效果,应该走差异化发展路径:第一,学校教育贯彻差异化教育理念,树立类型化评价观;第二,考试改革坚持培养差异化人才,建立类型化评价机制;第三,多主体落实差异化用人评价,营造类型化评价社会文化。

关键词：类型教育类型化评价教育评价改革差异化人才观

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Leptonic di-flavor and di-number violation processes at high energyμ^(±)μ^(±)colliders

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Chinese Physics C 2024年第4期48卷 34-50页

作者：杨金磊张肇西冯太傅 CAS Key Laboratory of Theoretical Physics Institute of Theoretical PhysicsChinese Academy of SciencesBeijing 100190China School of Physical Sciences University of Chinese Academy of SciencesBeijing 100049China School of Physical Science and Technology Lanzhou UniversityLanzhou 730000China Department of Physics Hebei UniversityKey Laboratory of High-precision Computation and Application of Quantum Field Theory of Hebei ProvinceBaoding 071002China

The leptonic di-flavor violation (LFV) processes μ^(±)μ^(±)→e^(±)e^(±) and μ^(±)μ^(±)→τ^(±)τ^(±) and leptonic di-number violation (LNV) processes μ^(±)μ^(±)→W_(i)^(±)W_(j)^(±) (i,j=1,2) at same-sign high energy μ^(±)μ^(±) colliders are studied. The new physics (NP) factors that may play roles in these processes are highlighted by cataloging them into three types. Taking into account the experimental constraints, the processes at μ^(±)μ^(±) colliders are computed, and the results are presented properly. The results lead to the conclusion that observing the NP factors through the LFV and LNV processes at TeV-energy μ^(±)μ^(±) colliders has significant advantages that cannot be achieved elsewhere. Therefore, once the techniques for muon acceleration and collision are developed successfully, the option of building same-sign high energy muon colliders should be seriously considered.

关键词： μ^(±)μ^(±)collider neutral lepton Majorana components

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Natural variation of GmFNSII-2 contributes to drought resistance by modulating enzyme activity in soybean

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The Crop Journal 2024年第2期12卷 529-539页

作者： Huihui Gao Pengcheng Wei Yongzhe Gu Pengbin Tang Yifan Shen lei yang Linxin Dong Haowei Zheng Kuo Shu Mayamiko Masangano Bin Dong Long Miao Jiajia Li Lijuan Qiu Xiaobo Wang School of Agronomy Anhui Agricultural UniversityHefei 230036AnhuiChina State Key Laboratory of Crop Gene Resources and Breeding The National Key Facility for Crop Gene Resources and Genetic Improvement(NFCRI)/Key Laboratory of Crop Gene Resource and Germplasm Enhancement(MOA)Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China

As an essential crop that provides vegetable oil and protein,soybean(Glycine max(L.)Merr.)is widely planted all over the ***,the scarcity of water resources worldwide has seriously impacted on the quality and yield of *** address this,exploring excellent genes for improving drought resistance in soybean is *** this study,we identified natural variations of GmFNSII-2(flavone synthase II)significantly affect the drought resistance of *** sequence analysis of GmFNSII-2 in 632 cultivated and 44 wild soybeans nine haplotypes were *** full-length allele GmFNSII-2^(C),but not the truncated allele GmFNSII-2^(A) possessing a nonsense nucleotide variation,increased enzyme *** research found that GmDREB3,known to increase soybean drought resistance,bound to the promoter region of GmFNSII-2^(C).GmDREB3 positively regulated the expression of GmFNSII-2^(C),increased flavone synthase abundance and improved the drought ***,a singlebase mutation in the GmFNSII-2^(C) promoter generated an additional drought response element(CCCCT),which had stronger interaction strength with GmDREB3 and increased its transcriptional activity under drought *** frequency of drought-resistant soybean varieties with Hap 1(Pro:GmFNSII-2^(C))has increased,suggesting that this haplotype may be selected during soybean *** summary,GmFNSII-2^(C) could be used for molecular breeding of drought-tolerant soybean.

关键词： Drought stress Flavone synthase GmFNSII-2 Natural variation Drought response element

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Laser-induced breakdown spectroscopy as a method for millimeter-scale inspection of surface flatness

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Plasma Science and Technology 2024年第9期26卷 148-155页

作者： Jinrui YE Yaju LI Zhao ZHANG Xinwei WANG Kewei TAO Qiang ZENG Liangwen CHEN Dongbin QIAN Shaofeng ZHANG lei yang Xinwen MA Institute of Modern Physics Chinese Academy of SciencesLanzhou 730000People’s Republic of China Advanced Energy Science and Technology Guangdong LaboratoryHuizhou 516000People’s Republic of China University of the Chinese Academy of Sciences Beijing 100049People’s Republic of China Key Laboratory of Atomic and Molecular Physics&Functional Materials of Gansu Province College of Physics and Electronic EngineeringNorthwest Normal UniversityLanzhou 730070People’s Republic of China Queen Mary University of London Engineering School Northwestern Polytechnical UniversityXi’an 710072People’s Republic of China

A non-contact method for millimeter-scale inspection of material surface flatness via Laser-Induced Breakdown Spectroscopy(LIBS)is investigated *** experiment is performed using a planished surface of an alloy steel sample to simulate its various flatness,ranging from 0 to 4.4 mm,by adjusting the laser focal plane to the surface distance with a step length of 0.2 *** is found that LIBS measurements are successful in inspecting the flatness differences among these simulated cases,implying that the method investigated here is *** is also found that,for achieving the inspection of surface flatness within such a wide range,when univariate analysis is applied,a piecewise calibration model must be *** is due to the complex dependence of plasma formation conditions on the surface flatness,which inevitably complicates the inspection *** solve the problem,a multivariate analysis with the help of Back-Propagation Neural Network(BPNN)algorithms is applied to further construct the calibration *** detailed analysis of the model performance,we demonstrate that a unified calibration model can be well established based on BPNN algorithms for unambiguous millimeter-scale range inspection of surface flatness with a resolution of about 0.2 mm.

关键词： laser-induced breakdown spectroscopy machine learning surface flatness

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后发内生:世界一流大学建设的中国智慧

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高教探索 2024年第5期 21-28页

作者：杨蕾广东技术师范大学教育科学学院广州510655 广东技术师范大学粤港澳大湾区职业教育现代化研究中心

世界一流大学建设的中国智慧是贯穿建设实践的创新性策略,是引领建设实践的思想和灵魂。从理念上看,中国的世界一流大学建设通过坚守自主内生的发展目标、发挥有为政府的发展动力、构建多源合流的发展资源,打破了现代化等于西方化的迷... 详细信息

世界一流大学建设的中国智慧是贯穿建设实践的创新性策略,是引领建设实践的思想和灵魂。从理念上看,中国的世界一流大学建设通过坚守自主内生的发展目标、发挥有为政府的发展动力、构建多源合流的发展资源,打破了现代化等于西方化的迷思。从实践上看,中国的世界一流大学建设在赶超目标上,致力于实现合目的性与和合规律性的统一;在赶超动力上,推动有为政府与有效市场相协调;在赶超资源上,形成借鉴创新与自主创新相结合的发展资源。在长期的探索过程中,逐渐形成了从“后发外生”向“后发内生”的路径转向。这些有益探索拓宽了后发国家世界一流大学建设路径,彰显了中国式高等教育现代化的力量。

关键词：世界一流大学建设中国智慧后发内生内源发展

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Solar image reconstruction method under atmospheric turbulence at Fuxian Lake Solar Observatory

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Astronomical Techniques and Instruments 2024年第2期1卷 128-139页

作者： Sizhong Zou Zhenyu Jin Kaifan Ji Jun Xu lei yang Yunnan Observatories Chinese Academy of SciencesKunming 650216China Yunnan Key Laboratory of Solar Physics and Space Science Kunming 650216China University of Chinese Academy of Sciences Beijing 101408China

Strong atmospheric turbulence reduces astronomical seeing,causing speckle images acquired by ground-based solar telescopes to become blurred and *** distortion in speckle images impedes image phase deviation in the speckle masking reconstruction method,leading to the appearance of spurious imaging *** only on linear image degradation principles to reconstruct solar images is *** solve this problem,we propose the multiframe blind deconvolution combined with non-rigid alignment(MFBD-CNRA)method for solar image *** consider image distortion caused by atmospheric turbulence and use non-rigid alignment to correct pixel-level distortion,thereby achieving nonlinear constraints to complement image intensity *** creating the corrected speckle image,we use the linear method to solve the wavefront phase,obtaining the target *** verify the effectiveness of our method results,compared with others,using solar observation data from the 1 m new vacuum solar telescope(NVST).This new method successfully reconstructs high-resolution images of solar observations with a Fried parameter r0 of approximately 10 cm,and enhances images at high *** r0 is approximately 5 cm,the new method is even more *** reconstructs the edges of solar graining and sunspots,and is greatly enhanced at mid and high frequency compared with other *** confirm the effectiveness of this method,with respect to both nonlinear and linear constraints in solar image *** provides a suitable solution for image reconstruction in ground-based solar observations under strong atmospheric turbulence.

关键词： Astronomical seeing Solar telescopes Solar observatories Astronomy image processing Phase error Deconvolution

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OsPRMT6a-mediated arginine methylation of OsJAZ1 regulates jasmonate signaling and spikelet development in rice

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Molecular Plant 2024年第6期17卷 900-919页

作者： Kun Dong Fuqing Wu Siqi Cheng Shuai Li Feng Zhang Xinxin Xing Xin Jin Sheng Luo Miao Feng Rong Miao Yanqi Chang Shuang Zhang Xiaoman You Peiran Wang Xin Zhang Cailin lei Yulong Ren Shanshan Zhu Xiuping Guo Chuanyin Wu Dong-lei yang Qibing Lin Zhijun Cheng Jianmin Wan State Key Laboratory of Crop Gene Resources and Breeding National Key Facility for Crop Gene Resources and Genetic ImprovementInstitute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China State Key Laboratory for Crop Genetics and Germplasm Enhancement Nanjing Agricultural UniversityNanjing 210095China

Although both protein arginine methylation(PRMT)and jasmonate(JA)signaling are crucial for regulating plant development,the relationship between these processes in the control of spikelet development remains *** this study,we used the CRISPR/Cas9 technology to generate two OsPRMT6a loss-of-function mutants that exhibit various abnormal spikelet ***,we found that OsPRMT6a can methylate arginine residues in JA signal repressors OsJAZ1 and *** showed that arginine methylation of OsJAZ1 enhances the binding affinity of OsJAZ1 with the JA receptors OsCOI1a and OsCOI1b in the presence of JAs,thereby promoting the ubiquitination of OsJAZ1 by the SCF^(OsCOI1a/OsCOI1b) complex and degradation via the 26S *** process ultimately releases OsMYC2,a core transcriptional regulator in the JA signaling pathway,to activate or repress JA-responsive genes,thereby maintaining normal plant(spikelet)***,in the osprmt6a-1 mutant,reduced arginine methylation of OsJAZ1 impaires the interaction between OsJAZ1 and OsCOI1a/OsCOI1b in the presence of *** a result,OsJAZ1 proteins become more stable,repressing JA responses,thus causing the formation of abnormal spikelet ***,we discovered that JA signaling reduces the OsPRMT6a mRNA level in an OsMYC2-dependent manner,thereby establishing a negative feedback loop to balance JA *** further found that OsPRMT6a-mediated arginine methylation of OsJAZ1 likely serves as a switch to tune JA signaling to maintain normal spikelet development under harsh environmental conditions such as high ***,our study establishes a direct molecular link between arginine methylation and JA signaling in rice.

关键词： protein arginine methylation jasmonate signaling negative feedback loop spikelet development adaptation to heat stress rice

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