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Chemometrics of differentially expressed proteins from colorectal cancer patients

World Journal of Gastroenterology 2011年第16期17卷 2096-2103页

作者： Lay-Chin Yeoh Saravanan Dharmaraj Boon-Hui Gooi Manjit Singh lay-Harn Gam School of Pharmaceutical Sciences Universiti Sains Malaysia Centre for Drug Research Universiti Sains Malaysia Department of Surgery Penang General Hospital

AIM:To evaluate the usefulness of differentially expressed proteins from colorectal cancer (CRC) tissues for differentiating cancer and normal tissues.METHODS:A Proteomic approach was used to identify the differentially expressed proteins between CRC and normal tissues.The proteins were extracted using Tris buffer and thiourea lysis buffer (TLB) for extraction of aqueous soluble and membrane-associated proteins,respectively.Chemometrics,namely principal component analysis (PCA) and linear discriminant analysis (LDA),were used to assess the usefulness of these proteins for identifying the cancerous state of tissues.RESULTS:Differentially expressed proteins identified were 37 aqueous soluble proteins in Tris extracts and 24 membrane-associated proteins in TLB extracts.Based on the protein spots intensity on 2D-gel images,PCA by applying an eigenvalue > 1 was successfully used to reduce the number of principal components (PCs) into 12 and seven PCs for Tris and TLB extracts,respectively,and subsequently six PCs,respectively from both the extracts were used for LDA.The LDA classification for Tris extract showed 82.7% of original samples were correctly classified,whereas 82.7% were correctly classified for the cross-validated samples.The LDA for TLB extract showed that 78.8% of original samples and 71.2% of the cross-validated samples were correctly classified.CONCLUSION:The classification of CRC tissues by PCA and LDA provided a promising distinction between normal and cancer types.These methods can possibly be used for identification of potential biomarkers among the differentially expressed proteins identified.

关键词：化学计量学方法蛋白质组学差异表达大肠癌组织状态可溶性蛋白患者线性判别分析

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Hydrophobic protein in colorectal cancer in relation to tumor stages and grades

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World Journal of Gastroenterology 2010年第22期16卷 2754-2763页

作者： Lay-Chin Yeoh Chee-Keat Loh Boon-Hui Gooi Manjit Singh lay-Harn Gam School of Pharmaceutical Sciences Universiti Sains Malaysia Department of Surgery Penang General Hospital

AIM: To identify differentially expressed hydrophobic proteins in colorectal cancer. METHODS: Eighteen pairs of colorectal cancerous tissues in addition to tissues from normal mucosa were analysed. Hydrophobic proteins were extracted from the tissues, separated using 2-D gel electrophoresis and analysed using Liquid Chromatography Tandem Mass Spectrometry (LC/MS/MS). Statistical analysis of the proteins was carried out in order to determine the significance of each protein to colorectal cancer (CRC) and also their relation to CRC stages, grades and patients’ gender. RESULTS: Thirteen differentially expressed proteins which were expressed abundantly in either cancerous or normal tissues were identified. A number of these proteins were found to relate strongly with a particular stage or grade of CRC. In addition, the association of these proteins with patient gender also appeared to be significant.CONCLUSION: Stomatin-like protein 2 was found to be a promising biomarker for CRC, especially in female patients. The differentially expressed proteins identified were associated with CRC and may act as drug target candidates.

关键词： Biomarker Colorectal cancer Liquid chromatography tandem mass spectrometry Proteomic approach 2D-polyacrylamide gel electrophoresis

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Hypoxia increases adipogenesis and affects adipocytokine production in orbital fibroblasts-a possible explanation of the link between smoking and Graves' ophthalmopathy

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International Journal of Ophthalmology(English edition) 2014年第3期7卷 403-407页

作者： Chiaw Ling Chng Oi Fah Lai Charmaine Sze-Min Chew Yu Pei Peh Stephanie Man-Chung Fook-Chong lay Leng Seah Daphne Hsu-chin Khoo Department of Endocrinology Singapore General HospitalLevel 3 the Academia 20 College Road 169856 Singapore Department of Clinical Research Academia Discovery Tower Level 9 Research Office 2 20 College Road 169856Singapore Oculoplastic/Aesthetic Eyeplastic Service Singapore National Eye Centre 11 Third Hospital Avenue 168751Singapore

AIM:To assess the effects of hypoxia on human orbital fibroblasts(OF)on adipogenesis and adipocytokine production.METHODS:Human OF were derived from tissues obtained from patients with Graves’ophthalmopathy(GO)and from patients without known thyroid diseases undergoing blepharoplasty.The OF were cultured separately under normoxic and hypoxic conditions.Comparisons of adipocytokine concentrations using multiplex ELISA and lipid accumulation in the cells using Oil Red O staining were subsequently performed.RESULTS:There was increased adipogenesis in OF from GO subject when exposed to hypoxic culture conditions.This was not observed in OF from normal controls.Hypoxia led to an increase in leptin and a decrease in MCP-1 secretion in OF cultures.CONCLUSION:Hypoxia induces adipogenesis in OF and may represent a mechanism by which smoking contributes to deterioration of GO.We also found novel changes to leptin and MCP-1 production in OF cultures exposed to hypoxia suggesting important roles of these cytokines in the disease process.

关键词： hypoxia smoking Graves' ophthalmopathy

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Key considerations on the development of biodegradable biomaterials for clinical translation of medical devices:With cartilage repair products as an example

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Bioactive Materials 2022年第3期7卷 332-342页

作者： Li Wang Xiaolei Guo Jiaqing Chen Zhen Zhen Bin Cao Wenqian Wan Yuandong Dou Haobo Pan Feng Xu Zepu Zhang Jianmei Wang Daisong Li Quanyi Guo Qing Jiang Yanan Du Jiakuo Yu Boon chin Heng Qianqian Han Zigang Ge Department of Biomedical Engineering College of EngineeringPeking UniversityBeijingPR China Center for Medical Device Evaluation National Medical Products AdministrationBeijingPR China Jiangsu DissueTech Medical Technology Co.Ltd|DeJian Group SuzhouPR China State Key Laboratory of High Performance Ceramics and Superfine Microstructure Shanghai Institute of CeramicsChinese Academy of SciencesShanghaiPR China Research Center for Human Tissue and Organs Degeneration Shenzhen Institute of Advanced TechnologyChinese Academy of ScienceShenzhenPR China Bioinspired Engineering and Biomechanics Center(BEBC) MOE Key Laboratory of Biomedical Information EngineeringSchool of Life Science and TechnologyXi’an Jiaotong UniversityXi’anPR China Beijing Institute of Science and Technology Evaluation BeijingPR China Institute of Orthopedics Chinese PLA General HospitalBeijingPR China Department of Sports Medicine and Adult Reconstructive Surgery State Key Laboratory of Pharmaceutical BiotechnologyThe Affiliated Nanjing Drum Tower Hospital of Nanjing University Medical SchoolNanjingPR China Department of Biomedical Engineering School of MedicineTsinghua UniversityBeijingPR China Knee Surgery Department of the Institute of Sports Medicine Beijing Key Laboratory of Sports InjuriesPeking University Third HospitalBeijingPR China School of Stomatology Peking UniversityBeijingPR China National Institutes for Food and Drug Control BeijingPR China Department of Biomedical Engineering Institute of Future TechnologyPeking UniversityBeijingPR China

With the interdisciplinary convergence of biology,medicine and materials science,both research and clinical translation of biomaterials are progressing at a rapid pace.However,there is still a huge gap between applied basic research on biomaterials and their translational products-medical devices,where two significantly different perspectives and mindsets often work independently and non-synergistically,which in turn significantly increases financial costs and research effort.Although this gap is well-known and often criticized in the biopharmaceutical industry,it is gradually widening.In this article,we critically examine the developmental pipeline of biodegradable biomaterials and biomaterial-based medical device products.Then based on clinical needs,market analysis,and relevant regulations,some ideas are proposed to integrate the two different mindsets to guide applied basic research and translation of biomaterial-based products,from the material and technical perspectives.Cartilage repair substitutes are discussed here as an example.Hopefully,this will lay a strong foundation for biomaterial research and clinical translation,while reducing the amount of extra research effort and funding required due to the dissonance between innovative basic research and commercialization pipeline.

关键词： Biomaterials Polymer Translation Medical devices Regulation Clinical needs

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Achieving high performance of Si-based anode materials for lithium ion battery via high energy mechanical milling

Achieving high performance of Si-based anode materials for l...

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第十一届海峡两岸超微颗粒学术研讨会暨中国颗粒学会超微颗...

作者： Jow-lay Huang Chia-chin Chang Shang-Chieh Hou Department of Materials Science and Engineering Cheng Kung University Hierarchical Green-Energy Materials Research Center Cheng Kung University Center for Micro/Nano Science and Technology Cheng Kung University Department of Greenergy University of Tainan Research and Development Center for Li-ion Battery University of Tainan

In this work,we have developed a robust,cost-effect and friendly to operators and environment process to synthesize the high performance of Si-based composite powder as negative electrode materials for lithium ion batteries(LIBs).The feature of this research includes construction of top-down method using high energy mechanical milling(HEMM) to transform micrometric Si powder of crystalline phase into nanocrystalline/amorphous phase,combining withwet milling in specific medium to further reduced size of Si aggregate,simultaneously transferring function group to the surface of Si resulted in enhancing formation of solid electrolyte interface upon lithiation/delithiation.Otherwise,Alloy designed Si/Cu3 Si composite powder is realized in all solid-state process by HEMM inducing mechanochemical reaction.Cu3 Si compound strengthens Si-based composite and enhances conductivity.Thus,Si-based composite powder as the negative electrode performs high electrochemical properties and approaches practical and industrial application for LIBs.

关键词： Lithium-ion batteries Si-based negative electrode high energy mechanical milling mechanochemical reaction

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转型升级背景下制造业人资管理实践与情感承诺:基于易学阴阳和谐视角

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管理评论 2018年第11期30卷 164-175页

作者：覃大嘉呼玲妍刘人怀杭州电子科技大学管理学院杭州310018 杭州电子科技大学创新与发展研究院杭州310012 暨南大学战略管理研究中心广州510632

中国制造业创新与转型的基础是劳动力升级,与人资管理实践息息相关,但过去结合中国文化与人力资本视角分析相关议题的研究较少。鉴于此,本文基于易学阴阳和谐理论,探讨在转型升级背景下,我国制造业人资管理实践与员工情感承诺间的作用... 详细信息

中国制造业创新与转型的基础是劳动力升级,与人资管理实践息息相关,但过去结合中国文化与人力资本视角分析相关议题的研究较少。鉴于此,本文基于易学阴阳和谐理论,探讨在转型升级背景下,我国制造业人资管理实践与员工情感承诺间的作用机制。研究团队调研了阿迪达斯7家中国区一线代工厂,对收集到的1 920组数据进行统计分析。结果发现,四项人资管理实践均对情感承诺有显著的正向影响,绩效考核影响最大,其次分别是公司培训、薪酬与福利管理,招聘与甄选影响最小;年龄能显著调节绩效考核对情感承诺的影响;绩效考核和薪酬与福利管理的交互作用,以及公司培训和招聘与甄选的交互作用,都会显著且正向地影响情感承诺。本文的主要理论贡献在于融合易学哲学思维与西方理论,探索特定背景下人资管理实践对职工情感承诺的影响,有助于进一步建构本土化、深度情境化的中国管理理论。此外,面对经济新常态的挑战,本研究可为所有在华制造业管理者的人力资本战略制定提供有价值的实证依据,具有一定的实践意义。

关键词：人力资源情感承诺制造业阴阳和谐文化

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植食性昆虫的食性和营养

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昆虫学报 1962年第2期 169-185页

作者：钦俊德

本文综述和讨论近年来有关植食性昆虫食性和营养的研究结果和发展。第一部分略述该类昆虫食物因理化性质的各异所引起的食性特化现象,由此所产生的研究课题和国际对这方面的研究动态。第二部分叙述昆虫的感觉作用和食料植物选择之间的关... 详细信息

本文综述和讨论近年来有关植食性昆虫食性和营养的研究结果和发展。第一部分略述该类昆虫食物因理化性质的各异所引起的食性特化现象,由此所产生的研究课题和国际对这方面的研究动态。第二部分叙述昆虫的感觉作用和食料植物选择之间的关系,列举对不同昆虫在觅食或产卵时有诱致或驱斥效应的和在取食时有助长或抑制效应的感觉刺激的本质和昆虫反应的特点。第三部分讨论植食性昆虫的营养和代谢的特点及其与食性特化的关系,在列举较近期间的研究成就后指出在不同种类中所存在着的营养差异,可能和食性特化有某些联系。这类昆虫的营养需要和对植物中有毒物质的适应都是代谢类型的反映,它们和昆虫的感觉反应有一定的联系,在与植物建立营养的关系中同样是重要的。第四部分叙述并讨论了有关植食性昆虫食性的某些理论,其中包括在选择食物中的机制、食性形成的主导因素、对食性类型以及食性演化的解释等。最后,指出对有关方面研究的可能进展和成就,及其在昆虫学各方面所起的影响。

关键词：植食性昆虫特化食料植物取食食性营养

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试论西夏语的■“一生补处”——西夏语、汉语、梵文对勘

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西夏研究 2010年第2期 61-76页

作者：林英津台湾中央研究院语言学研究所

本文主要撷取西夏语译《金光明经》的■"一生补处"(王静如1932:94),透过西夏、汉语、梵文对勘,尝试解明西夏语的■。进一步,将取得的解释施用于其他相关对译语料,则能回馈已经解读的世俗文献,如西夏文本《孙子兵法》《类林》之用■。关... 详细信息

本文主要撷取西夏语译《金光明经》的■"一生补处"(王静如1932:94),透过西夏、汉语、梵文对勘,尝试解明西夏语的■。进一步,将取得的解释施用于其他相关对译语料,则能回馈已经解读的世俗文献,如西夏文本《孙子兵法》《类林》之用■。关于西夏语的研究,我们经常会遭遇同一个西夏字,却在不同的翻译文本中对当不同的汉字、表征不同的句法语义。我们有必要深度解读夏汉对译文本,而不是仅仅给西夏字安上对译汉字;遂令同一个西夏字,在不同的翻译文本有不同的对译汉字、不同的语义。我们应该以语言学的辩证程序,尽可能准确诠释表象背后文献语言的语义。然后,我们才有坚实的基础,逐步建构西夏语的语法体系。

关键词：西夏语文语法文献解读穰tgiej^2

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The study of impedance response of Si3N4/TiC ceramic nanocomposite at the nanoscale by conductive atomic force microscopy

The study of impedance response of Si3N4/TiC ceramic nanocom...

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中国颗粒学会超微颗粒专委会2013年年会暨第八届海峡两...

作者： Jow-lay Huang ching-Huan Lee Hao-Chih Liu Horng-Hwa Lu Chang-An Wang Po-Jui Su Department of Materials Science and Engineering National Cheng Kung University State Key Lab of New Ceramics and Fine Processing Department of Materials Science and EngineeringTsinghua University Department of Mechanical Engineering National Chin-Yi University of Technology Department of Chemical and Materials Engineering National University of Kaohsiung

We have successfully developed SiN/TiC and SiN/TiN based nanocomposites,the electrochemical impedance spectroscopy was used to evaluate electricity at the macro- and nano-scale.The isolated distribution of TiN exists in SiN whereas SiN/TiC of the same amount of additives forms a conductive percolation network.AFM based technique with a conductive nano-probe was further performed to characterize the local impedance responses

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中国土壤有机碳密度和储量的估算与空间分布分析

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土壤学报 2004年第1期41卷 35-43页

作者：解宪丽孙波周慧珍李忠佩李安波南京师范大学地理科学学院南京210097 中国科学院南京土壤研究所南京210008

基于 1∶40 0万的《中华人民共和国土壤图》和第二次土壤普查数据 ,运用地理信息系统技术 ,对中国土壤有机碳密度及储量做出估算 ,并且分析了土壤有机碳密度的空间分布差异。结果表明 :10 0cm深度的土壤有机碳密度介于 1 19kgm- 2 到 17... 详细信息

基于 1∶40 0万的《中华人民共和国土壤图》和第二次土壤普查数据 ,运用地理信息系统技术 ,对中国土壤有机碳密度及储量做出估算 ,并且分析了土壤有机碳密度的空间分布差异。结果表明 :10 0cm深度的土壤有机碳密度介于 1 19kgm- 2 到 176 46kgm- 2 之间 ,2 0cm深度的土壤有机碳密度介于 0 2 7kgm- 2 到53 46kgm- 2 之间 ;10 0cm和 2 0cm深度的土壤有机碳储量分别为 84 4Pg (1Pg =10 15 g)和 2 7 4Pg ;土壤有机碳密度具有高度的空间变异性 ,东北地区、青藏高原的东南部、云贵高原等森林、草甸分布的地区有机碳密度最高 ,准噶尔盆地、塔里木盆地、阿拉善高原与河西走廊、柴达木盆地等沙漠化地区的土壤有机碳密度最低 ;土壤有机碳密度的空间分布主要受气候。

关键词：中国土壤有机碳密度储量计算空间分布

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