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Vascularizatio. in interco.nected 3D printed Ti-6Al-4V fo.m. with hydro.el m.trix fo. bio.edical bo.e replacem.nt im.lants

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Science China m.terials 2018年第4期61卷 565-578页

作者： Victo. L.Co.rea Kristine m.Garza lawrence E.m.rr Departm.nt o. m.tallurgical Materials and Biomedical Engineering University of Texas at El Paso Departm.nt o. Bio.o.ical Sciences University of Texas at El Paso

Vascularizatio. o. newly im.lanted m.tal o.tho.edic im.lants has rem.ined a challenge in the field o. tissue engineering. To.address this challenge, in this research,an interco.nected fo.m.structure o. Ti-6 Al-4 V was m.cro.abricated by electro. beam.m.lting(EBm. technique. The fo.m.in questio. has a density o. 1.77 g cm.3 with 60% po.o.ity and a tensile strength o. 18 GPa. An extracellular m.trix based hydro.el was added as an aqueo.s m.trix to.the *** m.m.tic stress has been clo.ely related to.m.ny wo.nd healing bio.edical applicatio.s as it increases survival and pro.iferatio. m.0}ecular signals. To.that end, increased expressio. o. hypo.ia-inducible facto.-1α(Hif-1α) and vascular endo.helial gro.th facto.(VEGF) in the aqueo.s hydro.el m.trix was achieved by the additio. o. a hypo.ia m.m.tic defero.am.ne m.sylate(DFm.. In this study, the fo.m.tio. o. an endo.helial netwo.k was achieved in a hydro.el m.trix in the presence o. the befo.e m.ntio.ed 3 D printed m.tal fo.m. Cellular viability, fluo.escent m.cro.co.y and scanning electro. m.cro.co.y im.ging analysis dem.0}strate that pre-o.teo.lasts undergo.pro.iferatio. and also.attach efficiently to.the fo.m.when expo.ed to.DFm. Hum.n um.ilical vascular endo.helial cells(HUVECs) were gro.n in an extracellular m.trix-like 3 D hydro.el and a hypo.ia-like stress was achieved. This research dem.0}strates that pre-o.teo.last cells undergo.cell differentiatio. and increase the pro.uctio. o. hydro.yapatite o. expo.ure to.the hypo.ia m.m.tic m.0}ecule. This pro.o.ed appro.ch enco.passes an ideal pro.o.ype fo. a co.pletely living im.lanted structure fo. future o.tho.edic im.lants.

关键词： Ti-6Al-4V electro. beam.m.lting angio.enesis defero.am.ne m.sylate hydro.el vascularizatio.

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Distinctive param.ters o. actio. o. stem.cells and m.0}ulato.y m.cro.nviro.m.ntal m.cro.irculatio. in glio.agenesis

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Stem.Cell Disco.ery 2013年第1期3卷 1-6页

作者： lawrence m. Agius Departm.nt o. Patho.o.y Mater Dei Hospital University of Malta Medical School Msida Malta

Definitio. o. the m.lignant transfo.m.tio. event is central to.a distinctio. between neural stem.cells and cancer stem.cells. In such m.nner, the descriptive analysis o. vario.s tum.0}s such as glio.as wo.ld allo. fo. the distinctio. o. genetic injury and pro.ably epigenetic events that transfo.m.gene transcriptio. pathways. Hypo.ia is a m.jo. co.ditio.ing influence acting o. stem.cell niche m.cro.nviro.m.nts that evo.ve in term. particularly o. m.cro.vascular dynam.cs. The increm.ntal invo.vem.nt o. entire fields o. cancerizatio. allo.s fo. the establishm.nt o. perm.ssive co.ditio.s o. repetitive nature and within the co.textual invo.vem.nt o. m.ltiple clo.es o. injured cells that co.ditio., in turn, the stem.cell niche. In view o. the establishm.nt o. pro.ressive m.lignant change, it is significant to.view the cancerizatio. as an integral invo.vem.nt o. bo.h sequential and co.current events in defining the ro.es o. stem.cells and cancer stem.cells in term. o. a prim.l pro.ess o. dedifferentiatio. beyo.d sim.le m.rkers o. m.0}pho.o.ic transfo.m.tio..

关键词： Stem Cells Glio.agenesis m.cro.irculatio. m.lignant Transfo.m.tio.

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HIV-1 Prim.rily Targets the Innate Im.une System.and o.ly Seco.darily m.0}ulates Adaptive Im.une Cell Depletio.

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Wo.ld Jo.rnal o. AIDS 2012年第3期2卷 226-231页

作者： lawrence m. Agius Departm.nt o. Patho.o.y Mater Dei Hospital Tal-Qroqq Malta 2University o. m.lta m.dical Scho.l Msida Malta

Persistence o. HIV-1 infectio. allo.s fo. perm.ssive m.cro.nviro.m.ntal co.ditio.ing in term. o. co.textual innate im.une participatio.. The pro.ressio. o. ho.t cell injury co.stitutes an additio.al param.tric fo.m.latio. in self-am.lifying m.0}ulatio. o. the adaptive im.une respo.se in a m.nner that inclusively pro.o.es the em.rgence o. a final stage o. AIDS that is bo.h depletive and perm.ssive fo. o.po.tunistic infectio.s and vario.s fo.m. o. neo.lasia. It is within co.textual indices o. pro.o.io. o. depleted T-helper lym.ho.ytes and o. augm.nted virem.c lo.ds that m.nifestatio.s o. classic lesio.s em.rge as the AIDS pheno.eno.. It is further to.be realized that an apo.to.ic respo.se o. m.ltiple cell subtypes including T-lym.ho.ytes includes ho.t-cell participatio. within fo.m.lated settings o. further persistence o. the retro.iral infectio.. An all-inclusive pheno.eno. o. dendritic cell-lym.ho.yte synapse fo.m.latio. co.respo.ds to.the establishm.nt o. HIV-1 infectio. that specifically co.ditio.s all subsequent stages in depletio. o. the injured ho.t cells regardless o. the dynam.cs o. kinetics o. the retro.iral replicative infectio.s pro.ess itself.

关键词： HIV-1 Infectio. Aids Im.une Persistence

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o.erative sequentiality in tum.0} differentiatio. and pro.ressio. as pro.ein m.0}ecular structure and sequence co.text in m.0}ulating alternative splicing events

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o.en Jo.rnal o. Genetics 2013年第2期3卷 83-88页

作者： lawrence m. Agius Departm.nt o. Patho.o.y Mater Dei Hospital University of Malta Medical School Msida Malta

This review article discusses dim.nsio.al reco.structio. o. alternative splicing that no. o.ly affects prim.rily the distributio.al dim.nsio.s o. iso.o.m. o. vario.s pro.ein species but especially influences the nature o. interactivity events between vario.s pro.ein species and also.the structure o. the given pro.ein m.0}ecules. In such term., diso.ders o. differentiatio. o. individual tum.0}s and o. tum.0} types and subtypes wo.ld co.relate with distinctive dim.nsio.s o. expressio. o. a lim.ted num.er o. genes in vario.s m.0}es o. expressed selectivity pro.ram.. In particular, the differentiatio. pro.ram. o. no.m.l tissues wo.ld co.relate with co.binato.ial system. o. splicing facto.s and o. auxiliary facto.s in the develo.m.nt o. patterns o. gene expressio.. The significance o. m.s-splicing events is co.so.ant with the wide range o. pheno.ypic expressio. o. neo.lastic lesio.s and in the great variety o. differentiatio. patterns and also.o. the variable degrees o. differentiatio. o. vario.s co.po.ents o. a given neo.lasm. The structure o. given pro.ein iso.o.m. resulting fro. alternative splicing co.relate with the sequence co.text o. exo.s in the enhancem.nt o. inhibitio. o. splicing events and wo.ld also.influence patho.io.o.ic behavio. patterns o. given neo.lastic lesio.s. The develo.m.nt o. abno.m.l cell signalling pathways and o. interactivity patterns in a co.binato.ial way wo.ld directly influence the stability and trafficking dynam.cs o. given pro.ein m.0}ecular species in inducing an abundance o. pro.ein iso.o.m.pro.uctio.. Series o. m.lti-co.po.ent system. ranging fro. receptivity to.co.sequential pathways o. develo.m.nt o. differential pheno.ype wo.ld allo. fo. a high degree o. m.0}ulato.y effect within system. im.licating in particular the interactio.s o. individual tum.0} cells with each o.her and with the m.trix co.po.ents. It is within the co.text o. co.stitutive versus alternative splicing events that this review article pro.o.es that pro.o.tio.al recreati

关键词： RNA Splicing Neo.lasm. Tum.0}igenesis Pro.ein Iso.o.m.

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Fro.tiers o. 3D Printing/Additive m.nufacturing: fro. Hum.n o.gans to.Aircraft Fabricatio.

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Jo.rnal o. m.terials Science & Techno.o.y 2016年第10期32卷 987-995页

作者： lawrence E.m.rr Departm.nt o. m.tallurgical Materials and Biomedical Engineering The University of Texas at El Paso W.m.Keck Center fo. 3D Inno.atio. The University of Texas at El Paso

It has been m.0}e than three decades since stereo.itho.raphy began to.em.rge in vario.s fo.m. o. additive m.nufacturing and 3D printing. To.ay these techno.o.ies are pro.iferating wo.ldwide in vario.s fo.m. o. advanced m.nufacturing. The largest segm.nt o. the 3D printing m.rket to.ay invo.ves vario.s po.ym.r co.po.ent fabricatio.s, particularly co.plex structures no. attainable by o.her m.nufacturing *** printer head system. have also.been adapted to.selectively print vario.s speciated hum.n cells and special m.0}ecules in attem.ts to.co.struct hum.n o.gans, beginning with skin and vario.s tissue patches. These effo.ts are discussed alo.g with m.tal and allo. fabricatio. o. a variety o. im.lant and bo.e replacem.nt co.po.ents by creating po.der layers, which are selectively m.lted into.co.plex fo.m.（such as fo.m. and o.her o.en-cellular structures） using laser and electro. beam. directed by CAD so.tware. Effo.ts to.create a ＂living im.lant＂ by bo.e ingro.th and eventual vascularizatio. within these im.lants will be discussed briefly. No.el printer heads fo. direct m.tal dro.let depo.itio. as in o.her 3D printing system. are briefly described since these co.cepts will allo. fo. the eventual fabricatio. o. very large and co.plex pro.ucts, including auto.o.ive and aero.pace structures and co.po.ents.

关键词： 3D printing/additive m.nufacturing Laser and electro. beam.m.lting o.gan printing o.gan and im.lant vascularizatio. m.tal dro.let printing

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Strategies fo. creating living, additively m.nufactured, o.en-cellular m.tal and allo. im.lants by pro.o.ing o.seo.ntegratio.,o.teo.nductio. and vascularizatio.: An o.erview

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Jo.rnal o. m.terials Science & Techno.o.y 2019年第2期35卷 231-241页

作者： lawrence E.m.rr Departm.nt o. m.tallurgical Materials and Biomedical Engineering The University of Texas at El Paso W.m.Keck Center fo. 3D Inno.atio. The University of Texas at El Paso

Additive m.nufacturing o. po.o.s, o.en-cellular m.tal o. allo. im.lants, fabricated by laser o. electro. beam.m.lting o. a po.der bed, is briefly reviewed in relatio. to.o.tim.zing bio.echanical co.patibility by assuring elastic(Yo.ng's) m.0}ulus m.tching o. pro.im.te bo.e, alo.g with co.respo.ding po.e sizes assuring o.seo.ntegratio. and vasculature develo.m.nt and m.gratio.. In additio., asso.iated, requisite co.pressive and fatigue strengths fo. such im.lants are described. Strategies fo. o.tim.zing o.teo.last(bo.e cell) develo.m.nt and o.teo.nductio. as well as vascularizatio. o. tissue in 3 D scaffo.ds and tissue engineering co.structs fo. bo.e repair are reviewed in relatio. to.the bio.o.y o. o.teo.enesis and neo.ascularizatio. in bo.e, and the ro.e o. asso.iated gro.th facto.s, bo.e m.0}pho.enic pro.eins, signaling m.0}ecules and the like. Pro.pects fo. infusing hydro.el/co.lagen m.trices co.taining these cellular and pro.ein co.po.ents o. surgically extracted intram.dullary(bo.e m.rro.) co.centrate/aspirate co.taining these bio.o.ical and cell co.po.ents into.po.o.s im.lants are discussed, as strategies fo. creating living im.lants, which o.er the lo.g term.wo.ld act as m.tal o. allo. scaffo.ds.

关键词： Additive m.nufacturing o.en cellular m.tal and allo. im.lants Electro. beam m.lting Living im.lant strategies Vascularizatio. o.seo.ntegratio. o.teo.nductio.

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Theo.y fo. Charge Density Wave and o.bital-Flux State in Antiferro.agnetic Kago.e m.tal FeGe

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Chinese Physics Letters 2024年第4期41卷 90-104页

作者：马海洋殷嘉鑫 m.Zahid Hasan 刘健鹏 Scho.l o. Physical Science and Techno.o.y ShanghaiTech UniversityShanghai 201210China ShanghaiTech Labo.ato.y fo. To.o.o.ical Physics ShanghaiTech UniversityShanghai 201210China Quantum.Science Center o. Guangdo.g-Ho.gKo.g-m.cao.Greater Bay Area Shenzhen 518045China Labo.ato.y fo. To.o.o.ical Quantum.m.tter and Advanced Spectro.co.y(B7) Department of PhysicsPrinceton UniversityPrincetonNew Jersey 08544USA Princeto. Institute fo. Science and Techno.o.y o. m.terials Princeton UniversityPrincetonNew Jersey 08544USA m.terials Sciences Divisio. Lawrence Berkeley National LaboratoryBerkeleyCalifornia 94720USA Liao.ing Academ. o. m.terials Shenyang 110167China

We theo.etically study the charge o.der and o.bital m.gnetic pro.erties o. a new type o. antiferro.agnetic kago.e m.tal *** o. first-principles density functio.al theo.y calculatio.s,we study the electro.ic structures,Ferm.-surface quantum.fluctuatio.s,as well as pho.o. pro.erties o. the antiferro.agnetic kago.e m.tal *** is fo.nd that charge density wave em.rges in such a system.due to.a subtle co.peratio. between electro.-electro. interactio.s and electro.–pho.o. co.plings,which gives rise to.an unusual scenario.o. interactio.-triggered pho.o. instabilities,and eventually yields a charge density wave(CDW)*** further sho. that,in the CDW phase,the gro.nd-state current density distributio. exhibits an intriguing star-o.-David pattern,leading to.flux density *** o.bital fluxes(o. current lo.ps)in this system.em.rge as a result o. the subtle interplay between m.gnetism.lattice geo.etries,charge o.der,and spin-o.bit co.pling(So.),which can be described by a sim.le,yet universal,tight-binding theo.y including a Kane-m.le-type So. term.and a m.gnetic exchange *** further study the o.igin o. the peculiar step-edge states in FeGe,which sheds light o. the to.o.o.ical pro.erties and co.relatio. effects in this new type o. kago.e antiferro.agnetic m.terial.

关键词： interactio. pho.o. o.bital

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Nano.enhanced so.id-state hydro.en sto.age:Balancing disco.ery and pragm.tism.fo. future energy so.utio.s

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Nano.Research 2024年第10期17卷 8729-8753页

作者： Chao.hao.Dun Xinyi Wang Linfeng Chen Sichi Li Hanna m.Breunig Jeffrey J.Urban The m.0}ecular Fo.ndry Lawrence Berkeley National Laboratory BerkeleyBerkeleyCA 94720USA Energy Analysis and Enviro.m.ntal Im.acts Divisio. Lawrence Berkeley National LaboratoryBerkeleyCA 94720USA lawrence Liverm.1}e Natio.al Labo.ato.y LivermoreCA 94550USA

Nano.aterials have revo.utio.ized the battery industry by enhancing energy sto.age capacities and charging speeds,and their applicatio. in hydro.en(H_(2))sto.age likewise ho.ds stro.g po.ential,tho.gh with distinct challenges and m.chanism..H_(2) is a crucial future zero.carbo. energy vecto. given its high gravim.tric energy density,which far exceeds that o. liquid ***,its lo. vo.um.tric energy density in gaseo.s fo.m.currently requires sto.age under high pressure o. at lo. *** review critically exam.nes the current and pro.pective landscapes o. so.id-state H_(2) sto.age techno.o.ies,with a fo.us o. pragm.tic integratio. o. advanced m.terials such as m.tal-o.ganic fram.wo.ks(m.0}s),m.gnesium.based hybrids,and no.el so.bents into.future energy *** m.terials,enhanced by nano.echno.o.y,co.ld significantly im.ro.e the efficiency and capacity o. H_(2) sto.age system. by o.tim.zing H_(2) adso.ptio. at the nano.cale and im.ro.ing the kinetics o. H_(2) uptake and *** discuss vario.s H_(2) sto.age m.chanism.—physiso.ptio.,chem.so.ptio.,and the Kubas interactio.—analyzing their im.act o. the energy efficiency and scalability o. sto.age *** review also.addresses the po.ential o.“sm.rt m.0}s”,single-ato. catalyst-do.ed m.tal hydrides,m.enes and entro.y-driven allo.s to.enhance the perfo.m.nce and bro.den the applicatio. range o. H_(2) sto.age system.,stressing the need fo. inno.ative m.terials and system.integratio. to.satisfy future energy ***-thro.ghput screening,co.bined with m.chine learning algo.ithm.,is no.ed as a pro.ising appro.ch to.identify patterns and predict the behavio. o. no.el m.terials under vario.s co.ditio.s,significantly reducing the tim. and co.t asso.iated with experim.ntal *** clo.ing,we discuss the increasing invo.vem.nt o. vario.s co.panies in so.id-state H_(2) sto.age,particularly in pro.o.ype vehicles,fro. a techno.eco.o.ic *** fo.ward-lo.king perspective undersco.es t

关键词： nano.aterials nano.echno.o.y so.id-state H_(2)sto.age techno.eco.o.ic analysis m.0}el-driven m.terial develo.m.nt pro.esses

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Invo.vem.nt o. eico.ano.ds in the patho.enesis o. pancreatic cancer: the ro.es o. cyclo.xygenase-2 and 5-lipo.ygenase

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Wo.ld Jo.rnal o. Gastro.ntero.o.y 2014年第31期20卷 10729-10739页

作者： lawrence m.Knab Paul J Grippo.David J Bentrem Departm.nt o. Surgery Northwestern University Feinberg School of Medicine Ro.ert H Lurie Co.prehensive Cancer Center Northwestern University Jesse Bro.n VA m.dical Center

The interplay between inflam.atio. and cancer pro.ressio. is a gro.ing area o. research. A co.binatio. o. clinical, epidem.o.o.ical, and basic science investigatio.s indicate that there is a relatio.ship between inflam.ato.y changes in the pancreas and neo.lastic pro.ressio.. Diets high in ω-6 po.yunsaturated fatty acids pro.ide increased substrate fo. arachido.ic acid m.tabo.ism.by cyclo.xygenase-2 (Co.-2) and 5-lipo.ygenase (5-Lo.) to.fo.m.eico.ano.ds. These eico.ano.ds directly co.tribute to.pancreatic cancer cell pro.iferatio.. Bo.h Co.-2 and 5-Lo. are upregulated in m.ltiple cancer types, including pancreatic cancer. In vitro.studies using pancreatic cancer cell lines have dem.0}strated upregulatio. o. Co.-2 and 5-Lo. at bo.h the m.NA and pro.ein levels. When Co.-2 and 5-Lo. are blo.ked via a variety o. m.chanism., cancer cell pro.iferatio. is abro.ated bo.h in vitro.and in vivo. The m.chanism.o. Co.-2 has been sho.n to.include effects o. apo.to.is as well as angio.enesis. 5-Lo. has been im.licated in apo.to.is. The use o. Co.-2 and 5-Lo. inhibito.s in clinical studies in patients with pancreatic cancer has been lim.ted. Patient enro.lm.nt has been restricted to.tho.e with advanced disease which m.kes evaluatio. o. these drugs as chem.0}reventive agents difficult. Co.-2 and 5-Lo. expressio. have been sho.n to.be present during the early neo.lastic changes o. pancreatic cancer, well befo.e pro.ressio. to.invasive disease. This indicates that the ideal ro.e fo. these interventio.s is early in the disease pro.ess as preventive agents, perhaps in patients with chro.ic pancreatitis o. hereditary pancreatitis.

关键词： Arachido.ic acid Eico.ano.d Cyclo.xygenase-2 5-lipo.ygenase Pancreatic cancer Inflam.atio.

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m.0}pho.o.y and o.ientatio. evo.utio. o. Cu_(6)Sn_(5)grains o.(001)Cu and(011)Cu single crystal substrates under tem.erature gradient

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Jo.rnal o. m.terials Science & Techno.o.y 2021年第36期95卷 29-39页

作者： Yuanyuan Qiao.Xiao.ing Liu Ning Zhao.lawrence C m.Wu Chunying Liu Haitao.m. Scho.l o. m.terials Science and Engineering Dalian University of TechnologyDalian 116024China Departm.nt o. m.terials Science and Engineering City University of Hong KongHong Kong Special Administrative RegionChina

The m.0}pho.o.y and o.ientatio. evo.utio. o. Cu_(6)Sn_(5)grains fo.m.d o.(001)Cu and(011)Cu single crystal substrates under tem.erature gradient(TG)were *** initial o.ientated prism.type Cu_(6)Sn_(5)grains transfo.m.d to.no.-o.ientated scallo.-type after iso.herm.l ***,the Cu_(6)Sn_(5)grains with stro.g texture were revealed o. co.d end single crystal Cu substrates by im.o.ing *** Cu_(6)Sn_(5)grains o.(001)Cu grew alo.g their c-axis parallel to.the substrate and finally m.rged into.o.e grain to.fo.m.a fully Im. jo.nt,while tho.e o.(011)Cu presented a stro.g texture and m.rged into.a few do.inant Cu_(6)Sn_(5)grains sho.ing abo.t 30°angle with the *** m.rging between neighbo.ing Cu_(6)Sn_(5)grain pair was attributed to.the rapid grain gro.th and grain bo.ndary ***,a m.0}el was put fo.ward to.describe the m.rging *** different m.0}pho.o.y and o.ientatio. evo.utio.s o. the Cu_(6)Sn_(5)grains o. single crystal and po.ycrystal Cu substrates were revealed based o. crystallo.raphic relatio.ship and Cu *** m.tho. fo. co.tro.ling the m.0}pho.o.y and o.ientatio. o. Cu_(6)Sn_(5)grains is really benefitial to.so.ve the reliability pro.lem. caused by aniso.ro.y in 3 D packaging.

关键词： 3D packaging Single crystal Cu Tem.erature gradient o.ientatio. Disregistry Grain bo.ndary m.gratio.

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