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Spatial and Temporal Variation of Stable Isotopes in Precipitation across Costa Rica: An Analysis of Historic GNIP Records

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Open Journal of Modern Hydrology 2013年第4期3卷 226-240页

作者： Ricardo Sánchez-Murillo Germain Esquivel-Hernández Kristen Welsh Erin S. Brooks Jan Boll rosa Alfaro-Solís Juan Valdés-González department of Biological and Agricultural Engineering University of Idaho Moscow USA. Escuela de Química Universidad Nacional Heredia Costa Rica laboratorio de Química de la Atmósfera Escuela de Química Universidad Nacional Heredia Costa Rica Waters of the West-Water Resources Program University of Idaho Moscow USA

The location of Costa Rica on the Central American Isthmus creates unique microclimate systems that receive moisture inputs directly from the Caribbean Sea and the Pacific Ocean. In Costa Rica, stable isotope monitoring was conducted by the International Atomic Energy Agency and the World Meteorological Association as part of the worldwide effort entitled Global Network of Isotopes in Precipitation. Sampling campaigns were mainly comprised of monthly-integrated samples during intermittent years from 1990 to 2005. The main goal of this study was to determine spatial and temporal isotopic variations of meteoric waters in Costa Rica using historic records. Samples were grouped in four main regions: Nicoya Peninsula (d2H = 6.65d18O -0.13;r2 = 0.86);Pacific Coast (d2H = 7.60d18O + 7.95;r2 = 0.99);Caribbean Slope (d2H = 6.97d18O + 4.97;r2 = 0.97);and Central Valley (d2H = 7.94d18O + 10.38;r2 = 0.98). The water meteoric line for Costa Rica can be defined as d2H = 7.61d18O + 7.40 (r2 = 0.98). The regression of precipitation amount and annual arithmetic means yields a slope of ﹣1.6‰ d18O per 100 mm of rain (r2 = 0.57) which corresponds with a temperature effect of ﹣0.37‰ d18O/°C. A strong correlation (r2 = 0.77) of ﹣2.0‰ d18O per km of elevation was found. Samples within the Nicoya Peninsula and Caribbean lowlands appear to be dominated by evaporation enrichment as shown in d-excess interpolation, especially during the dry months, likely resulting from small precipitation amounts. In the inter-mountainous region of the Central Valley and Pacific slope, complex moisture recycling processes may dominate isotopic variations. Generally, isotopic values tend to be more depleted as the rainy season progresses over the year. Air parcel back trajectories indicate that enriched isotopic compositions both in Turrialba and Monteverde are related to central Caribbean parental moisture and low rainfall intensities. depleted events appear to be related to high rainfall amounts despite the

关键词： Stable Isotopes Costa Rica Precipitation GNIP HYSPLIT Model

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Chronic hepatitis C:This and the new era of treatment

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World Journal of Hepatology 2016年第2期8卷 92-106页

作者： Gaetano Bertino Annalisa Ardiri Maria Proiti Giuseppe Rigano Evelise Frazzetto Shirin demma MariaIrene Ruggeri laura Scuderi Giulia Malaguarnera Nicoletta Bertino Venerando Rapisarda Isidoro Di Carlo Adriana Toro Federico Salomone Mariano Malaguarnera Emanuele Bertino Michele Malaguarnera Hepatology Unit-department of Clinical and Experimental Medicine University of Catania95123 CataniaItaly Internal Medicine Unit ARNAS Civic Hospital90142 PalermoItaly Research Centre“la Grande Senesce” University of Catania95100 CataniaItaly department of Biomedical Sciences University of Catania95100 CataniaItaly Faculty of Pharmacy University of Catania95123 CataniaItaly Occupational Medicine Unit Department of Clinical and Experimental MedicineUniversity of Catania95100 CataniaItaly department of Surgical Sciences Organ Transplantation and Advanced TechnologiesUniversity of Catania95100 CataniaItaly Gastroenterology Unit Acireale Hospital95024 AcirealeCataniaItaly

Over the last years it has started a real revolution in the treatment of chronic hepatitis C. This occurred for the availability of direct-acting antiviral agents that allow to reach sustained virologic response in approximately 90% of cases. In the near future further progress will be achieved with the use of pan-genotypic drugs with high efficacy but without side effects.

关键词： Direct-acting antiviral agents Nucleosideinhibitors Boceprevir Sofosbuvir Telaprevir HepatitisC Simeprevir Daclatasvir Ledipasvir Faldaprevir Ritonavir Ombitasvir Dasabuvir

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Adjuvants derived from <i>Neisseria meningitidis</i>Serogroup B Induce a Cross Reactive Response against <i>Neisseria gonorrhoeae</i>in Mice

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Open Journal of Immunology 2020年第3期10卷 47-58页

作者： laura M. Reyes Miriam lastre Maribel Cuello Osmir Cabrera Yusnaby Borrego Raúl Ramos Pupo Steve Black Oliver Pérez department of Immunology Instituto de Ciencias Básicas y Preclínicas “Victoria de Girón” Universidad de Ciencias Médicas de la Habana Havana Cuba Facultad de Ingenierías Universidad Técnica “Luis Vargas Torres” de Esmeraldas Esmeralda Ecuador Biomedical Research Institute (BIOMED). Faculty of Medicine and Life Sciences Hasselt University Hasselt Belgium Cincinnati Children’s Hospital Cincinnati USA

Introduction: Neisseria is a large genus of bacteria that colonize mucosal surfaces. Of the 11 species that colonize humans, only two are pathogens, N. meningitidis and N. gonorrhoeae. Both are obligate human pathogens;the last is causal agent of gonorrhea disease. Although curable with timely antibiotic treatment, an annual incidence of more than 62 million cases is estimated by the World Health Organization and there are no successful vaccines available. In contrast, several prophylactic vaccine options for Neisseria meningitidis meningitis do exist. Of note, there is trace of cross parenteral response induced between N. meningitidis and N. gonorrhoeae, and Proteoliposome (PL, also named outer membrane vesicles, OMV) vaccine has shown high impact on this response. Objective: To determine effect of VAMENGOC-BC? and its derivates (AFPL1 and AFCo1) at mucosal and systemic level and possible cross response against Neisseria gonhorroeae in Balb/c and C57Bl/6 mice. Methods: We evaluated cross response against N. gonorrhoeae in mouse serum IgG and saliva IgA after mucosal immunization with VA-MENGOC-BC or its derivatives (AF, Adjuvant Finlay PL1 or AFCo (cochleate) 1). Results: Immunizations with AFPL1 or AFCo1 induce anti N. gonorrhoeae at saliva IgA and serum IgG responses similar to VA-MENGOC-BC? vaccine. Conclusions: Such data confirms a new possible window of prime-boost vaccination strategy against gonorrhea and extends our knowledge regarding the effect of PL vaccines on cross responses.

关键词： N. meningitidis N. gonorrhoeae Vaccine Cross Reactivity

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西班牙渗出性老年性黄斑变性的相关流行病学因素(英文)

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国际眼科杂志 2008年第6期8卷 1070-1072页

作者： Javier A Montero Hortensia Sanchez-Tocino Maria Jo-sefa Marti Maldonado Concepcién Arnaiz Nifio rosa Mafia Perez Bartolomé Antonio Valera Coomonte 西班牙巴利亚多利德Piodel Rio Hortega大学医院眼科西班牙阿利坎特眼科研究所视网膜科西班牙Castilla la Mancha大学阿利坎特医学院

目的：报告在西班牙Castilla-Leo地区患有渗出性老年性黄斑变性（AMD）的样本人群的流行病学特点，并与一组来自同一地区和相同年龄人群进行比较。方法：在本次观察性前瞻性调查中，我们访问了在一家地区性诊所接受光动力学疗法的AMD患... 详细信息

目的：报告在西班牙Castilla-Leo地区患有渗出性老年性黄斑变性（AMD）的样本人群的流行病学特点，并与一组来自同一地区和相同年龄人群进行比较。方法：在本次观察性前瞻性调查中，我们访问了在一家地区性诊所接受光动力学疗法的AMD患者。患者报告了高血压、高血脂、吸烟的病史，检查患者虹膜颜色并分为浅色（绿色、蓝色、灰色）和深色（淡褐色、褐色、黑色）。结果：共有343名患者接受了访问，平均年龄为74．9岁（55～93岁），其中64．5％为女性，35．5％为男性，有45．1％的患者虹膜颜色被划为浅色。这些患者中，有50％有动脉性高血压，15．3％接受高胆固醇血症治疗，30．2％吸烟或者已经戒烟（女性中占80．9％）。结论：在渗出性AMD患者中，虹膜颜色为浅色的频率较高。在我们的调查中，其他渗出性AMD其他的风险因素有男性的吸烟习惯、未接受高胆固醇血症治疗、患者为女性身份等。

关键词：渗出性老年性黄斑变性虹膜颜色吸烟血压高血脂吸烟史

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In vitro intracellular IFNγ, IL-17 and IL-10 producing T cells correlates with the occurrence of post-transplant opportunistic infection in liver and kidney recipients

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World Journal of Transplantation 2018年第1期8卷 23-37页

作者： Francisco Boix Santiago Llorente Jorge Eguía Gema Gonzalez-Martinez Rafael Alfaro Jose A Galián Jose A Campillo María rosa Moya-Quiles Alfredo Minguela Jose A Pons Manuel Muro department of Immunology Clinical University Hospital Virgen de la Arrixaca-IMIBClinical University Hospital‘Virgen Arrixaca’ department of Nephrology Clinical University Hospital‘Virgen de la Arrixaca-IMIB’Clinical University Hospital‘Virgen Arrixaca’ Digestive Medicine Service Clinical University Hospital‘Virgen de la Arrixaca-IMIB’Clinical University Hospital‘Virgen Arrixaca’

AIM To validate intracellular cytokine production functional assay as means of cell-mediated immunity monitoring of post-transplant patients with opportunistic infection(OI).METHODS Intracellular cytokine-producing CD4^+ and CD8^+ T-cell monitoring was carried out in 30 liver transplant(LTr) and 31 kidney transplant(KTr) recipients from 2010 to 2012. Patients were assessed in our department of Immunology at the Clinical University ‘Hospital Virgen de la Arrixaca-IMIB' in Murcia, Spain for one year following transplantation. FACS Canto Ⅱ flow cytometer was employed to quantify the intracellular production of IL-17, IFNγ and IL-10 cytokines on stimulated CD4^+CD69^+ and CD8^+CD69^+ T cells and BD FACS DIVA v.6 software was used to analysed the data. Statistical analysis was carried out using SPSS *** LTr with OI had significantly lower % of CD8^+CD69^+IFNγ^+T cells at 60(7.95 ± 0.77 vs 26.25 ± 2.09, P lantation. Higher % of CD4^+CD69^+IL-10^+ as well as CD4^+CD69^+IL-17^+ T cells were yet reported at 30(14.06 ± 1.65 vs 6.09 ± 0.53, P = 0.0007 and 4.23 ± 0.56 vs 0.81 ± 0.14, P = 0.005; respectively), 60(11.46 ± 1.42 vs 4.54 ± 0.91, P = 0.001 and 4.21 ± 0.59 vs 1.43 ± 0.42, P = 0.03; respectively) and 90 d(16.85 ± 1.60 vs 4.07 ± 0.63, P lantation as opposed to CD4^+CD69^+IL-10^+ and CD8^+CD69^+IL-10^+ T cells which percentages were higher at 30(25.21 ± 2.74 vs 8.54 ± 1.64, P < 0.001 and 22.37 ± 1.35 vs 17.18 ± 3.54, P = 0.032; respectively), 90(16.85 ± 1.60 vs 4.07 ± 0.63, P < 0.001 and 23.06 ± 2.89 vs 10.19 ± 1.98, P = 0.002) and 18

关键词： Intracellular cytokine Liver transplantation Kidney transplantation Opportunistic infection

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Phased,chromosome-scale genome assemblies of tetraploid potato reveal a complex genome,transcriptome,and predicted proteome landscape underpinning genetic diversity

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Molecular Plant 2022年第3期15卷 520-536页

作者： Genevieve Hoopes Xiaoxi Meng John P.Hamilton Sai Reddy Achakkagari Fernanda de Alves Freitas Guesdes Marie E.Bolger Joseph J.Coombs Danny Esselink Natalie R.Kaiser Linda Kodde Maria Kyriakidou Brian lavrijssen Natascha van Lieshout Rachel Shereda Heather K.Tuttle Brieanne Vaillancourt Joshua C.Wood Jan Mde Boer Nolan Bornowski Peter Bourke David Douches Herman Jvan Eck Dave Ellis Max J.Feldman Kyle M.Gardner Johannes C.P.Hopman Jiming Jiang Walter S.de Jong Joseph C.Kuhl Richard G.Novy Stan Oome Vidyasagar Sathuvalli Ek Han Tan Remco A.Ursum M.Isabel Vales Kelly Vining Richard G.F.Visser Jack Vossen G.Craig Yencho Noelle L.Anglin Christian W.B.Bachem Jeffrey B.Endelman laura M.Shannon Martina V.Stromvik Helen H.Tai Bjorn Usadel C.Robin Buell Richard Finkers department of Plant Biology Michigan State UniversityEast LansingMI 48824USA department of Horticultural Science University of MinnesotaSt.PaulMN 55108USA department of Plant Science McGill UniversitySainte-Anne-de-BellevueQC H9X 3V9Canada Plant Breeding Wageningen University&ResearchPlant Breeding6708 PB Wageningenthe Netherlands IBG-4 Bioinformatics Forschungszentrum JülichWilhelm Johnen Str52428 JülichGermany department of Plant Soiland Microbial SciencesMichigan State UniversityEast LansingMI 48824USA Bayer Crop Science WoodlandCA 95695USA Averis Seeds B.V. 9640 AA Veendamthe Netherlands International Potato Center 1895 Avenida La MolinaLimaPeru USDA-ARS ProsserWA 99350USA Agriculture and Agri-Food Canada Fredericton Research and development Centre FrederictonNB E3B 4Z7Canada School of Integrative Plant Science Cornell UniversityIthacaNY 14853-1901USA department of Plant Sciences University of IdahoMoscowID 83844USA USDA-ARS Small Grains and Potato Germplasm ResearchAberdeenID 83210USA HZPC Research B.V. Edisonweg 58501 XG Jourethe Netherlands department of Crop and Soil Science Oregon State UniversityHermistonOR 97838USA School of Biology and Ecology University of Maine5735 Hitchner Hall OronoME 04469USA department of Horticultural Sciences Texas A&M UniversityCollege StationTX 77843-2133USA department of Horticulture Oregon State UniversityCorvallisOR 97331USA department of Horticultural Science North Carolina State UniversityRaleighNC 27695-7609USA department of Horticulture University of Wisconsin-MadisonMadisonWI 53706USA department of Horticulture Michigan State UniversityEast LansingMI 48824USA Institute for Biological Data Science Heinrich Heine UniversityDusseldorf40225 DusseldorfGermany Plant Resilience Institute Michigan State UniversityEast LansingMI 48824USA Present address:GenNovation B.V. Wageningenthe Netherlands

Cultivated potato is a clonally propagated autotetraploid species with a highly heterogeneous *** assemblies of six cultivars including two chromosome-scale phased genome assemblies revealed extensive allelic diversity,including altered coding and transcript sequences,preferential allele expression,and structural variation that collectively result in a highly complex transcriptome and predicted proteome,which are distributed across the homologous *** species contribute to the extensive allelic diversity in tetraploid cultivars,demonstrating ancestral introgressions predating modern breeding *** a clonally propagated autotetraploid that undergoes limited meiosis,dysfunctional and deleterious alleles are not purged in tetraploid *** a quarter of the loci bore mutations are predicted to have a high negative impact on protein function,complicating breeder’s efforts to reduce genetic *** StCDF1 locus controls maturity,and analysis of six tetraploid genomes revealed that 12 allelic variants of StCDF1 are correlated with maturity in a dosage-dependent *** of the complexity of the tetraploid potato genome with its rampant structural variation and embedded deleterious and dysfunctional alleles will be key not only to implementing precision breeding of tetraploid cultivars but also to the construction of homozygous,diploid potato germplasm containing favorable alleles to capitalize on heterosis in F1 hybrids.

关键词： potato allele diversity phased assembly polyploid pan-genome wild introgressions

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In vitro Antibacterial Activity of Moringa oleifera Ethanolic Extract against Tomato Phytopathogenic Bacteria

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Phyton-International Journal of Experimental Botany 2021年第3期90卷 895-906页

作者： Roberto Arredondo-Valdés Francisco D.Hernández-Castillo Mario Rocandio-Rodríguez Julia C.Anguiano-Cabello Madai rosas-Mejía Venancio Vanoye-Eligio Salvador Ordaz-Silva Imelda V.López-Sánchez laura D.Carrazco-Peña Julio C.Chacón-Hernández Institute of Applied Ecology Universidad Autónoma de TamaulipasCiudad VictoriaTamaulipas87019México Parasitology department Universidad Autónoma Agraria Antonio NarroSaltillo25315México Health Area Universidad La Salle SaltilloSaltillo25298México Faculty of Engineering and Business San Quintin Universidad Autónoma de Baja CaliforniaSan Quintín22930México

The tomato(Solanum lycopersicum L.)is one of the world’s most important vegetable ***,phytopathogenic bacteria affect the yield and quality of tomato cultivation,like Agrobacterium tumefeciens(At),Clavibacter michiganensis ***(Cmm),Pseudomonas syringae ***(Pst),Ralstonia solanacearum(Rs),and Xanthomonas axonopodis(Xa).Synthetic chemical products are used mostly on disease plant control,but overuse generates resistance to bacterial *** study aimed to evaluate the in vitro antibacterial activity of the ethanolic extract of Moringa oleifera *** against At,Cmm,Pst,Rs,and Xa,as well as information about this plant species’chemical *** activity against pathogens observed by microplate technique,phytochemical screening,and FTIR analysis revealed different bio-active compounds on ethanolic extracts with antibacterial *** growth inhibition rate ranged between 0.08%and 99.94%.The inhibitory concentration,IC50,required to inhibit 50%of At,Cmm,Pst,Rs,and Xa bacterial growth,was 276.67,350.48,277.85,351.49,and 283.22 mg/L,*** of phytopathogen bacteria’s growth increased as the concentrations of the extract also *** oleifera extract can be recommended as a potent bio-bactericide.

关键词： Biological control secondary metabolites tomato crop inhibitory concentration botanical extract

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The regenerative response of cardiac interstitial cells

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Journal of Molecular Cell Biology 2022年第10期14卷 19-36页

作者： laura Rolland Alenca Harrington Adèle Faucherre Jourdano Mancilla Abaroa Girisaran Gangatharan laurent Gamba Dany Severac Marine Pratlong Thomas Moore-Morris Chris Jopling Institute of Functional Genomics University of MontpellierCNRSINSERMLabEx ICSTMontpellier 34094France Montpellier GenomiX France GénomiqueMontpellier 34094France

Understanding how certain animals are capable of regenerating their hearts will provide much needed insights into how this process can be induced in humans in order to reverse the damage caused by myocardial ***,it is becoming increasingly evident that cardiac interstitial cells play crucial roles during cardiac *** understand how interstitial cells behave during this process,we performed single-cell RNA sequencing of regenerating zebrafish *** a combination of immunohistochemistry,chemical inhibition,and novel transgenic animals,we were able to investigate the role of cell type-specific mechanisms during cardiac *** approach allowed us to identify a number of important regenerative processes within the interstitial cell ***,we provide detailed insight into how interstitial cells behave during cardiac regeneration,which will serve to increase our understanding of how this process could eventually be induced in humans.

关键词： heart regeneration interstitial cells single-cell RNA-sequencing macrophage fibroblast endothelium inflammation

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Severe COVID-19 after liver transplantation, surviving the pitfalls of learning on-the-go: Three case reports

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World Journal of Hepatology 2020年第10期12卷 870-879页

作者： Felipe Alconchel Pedro A Cascales-Campos Jose A Pons María Martínez Josefa Valiente-Campos Urszula Gajownik María L Ortiz laura Martínez-Alarcón Pascual Parrilla Ricardo Robles Francisco Sánchez-Bueno Santiago Moreno Pablo Ramírez department of Surgery and Organ Transplantation Virgen de la Arrixaca University HospitalMurcia 30120Spain department of Digestive Endocrine and Abdominal Surgery and TransplantationBiomedical Research Institute of Murcia(IMIBVirgen de la Arrixaca)Murcia 30120Spain department of Hepatology Virgen de la Arrixaca University HospitalMurcia 30120Spain department of Intensive Care Unit Virgen de la Arrixaca University HospitalMurcia 30120Spain department of Infectious Diseases Ramon y Cajal HospitalMadrid 28034Spain

BACKGROUND The novel coronavirus 2019(COVID-19)pandemic has dramatically transformed the care of the liver transplant *** patients who are immunosuppressed and with multiple comorbidities,severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection has been associated with increased severity and *** main objective of this report is to communicate our experience in the therapeutic management of SARS-CoV-2 infection in 3 liver transplant ***,we stress the management and investigation of the contagious spreading into a liver transplant *** SUMMARY The patients were two women(aged 61 years and 62 years)and one man(aged 68 years),all of them having recently received a liver *** three patients required intensive care unit admission and invasive mechanical *** of them progressed severely until *** other one,who received tocilizumab,had a good *** the outbreak,the wife of one of the patients and four healthcare professionals involved in their care were also *** We illustrate in detail the evolution of a nosocomial COVID-19 outbreak in a liver transplant *** believe that these findings will contribute to a better understanding of the natural history of the disease and will improve the treatment of the liver transplant patient with COVID-19.

关键词： Liver transplantation COVID-19 SARS-CoV-2 Cross infection Nosocomial infection Case report

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Classical and Non-Classical Celiac Disease Comparison: Ten Years of Study

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Open Journal of Pediatrics 2022年第2期12卷 309-319页

作者： Katia Regina Pena Schesquini-Roriz Jocelyn Cristina Betancourt Castellanos laura Martinez Martinez Gloria Maria Fraga Rodriguez Susana Boronat Guerrero Isabel Badell Serra department of Pediatrics Hospital de La Santa Creu I Sant Pau Universitat Autònoma de Barcelona Barcelona Spain department of Medicine Federal University of Rond&ocirc nia Porto Velho Brazil

Objective: Celiac disease (CD) is an immune-mediated systemic disorder triggered by gluten. It has a variable combination of clinical manifestations and changes that have been occurring in recent decades however they are not known in detail. The purpose of the article is to compare Classical and Non-Classical CD cases in terms of demographic characteristics, duodenal biopsy, extraintestinal manifestations, and associated comorbidities. Materials and Methods: A comparative retrospective cohort study from January 2008 to december 2018. Results: A total of 128 cases were included: 84 Classical (66%) and 44 Non-Classical CD (34%). The family history of CD was identified in 14% of cases without differences between groups. The age at diagnosis was distinct for Classical and Non-Classical CD (4.9 ± 4 and 8.3 ± 4 years old;p 0.001), respectively. Important changes were found within the classical presentation, including mono symptoms and a significantly higher rate of intestinal atrophy;p = 0.04. The main Non-Classical CD symptom was recurrent abdominal pain. The extraintestinal manifestations (EIM) were identified in 42% and occurred in both groups. The comparison between groups showed differences in rates of migraine and vitamin D deficiency and was higher for Non-Classical CD (p 0.05). Associated diseases occurred in 10.9%, and type 1 diabetes was significant for the Non-Classical CD group (p = 0.04). Conclusion: The classical CD was the most prevalent profile and presented a decrease in the severity of symptoms however remain a higher rate of intestinal atrophy. Recurrent abdominal pain was the main symptom of Non-Classical CD. Extraintestinal manifestations and associated diseases presented an increasing trend of occurrence among cases of Non-Classical CD.

关键词： Celiac Disease Gluten Allergy Extra-Intestinal Manifestation Disease Associated with Celiac Disease

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