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Effects of Estradiol on 5-HTsA and 5-HT2c Receptor Immunolabeling in Rat Hippocampus

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Journal of Life Sciences 2011年第11期5卷 884-889页

作者： laura cristina berumen Marco Antonio Sainchez-Ramos Martin Garcla-Servin Ataulfo Martinez-Torres Angelina Rodriguez Guadalupe Garcla-Alcocer Facultad de Quimica Universidad A utonoma de Querotaro Centro Universitario Queretaro 76010 Mexico Facultad de Ciencias Naturales Universidad Autonoma de Queretaro Campus Juriquilla Juriquilla Queretaro 76230 Mexico Department of Cellular and Molecular Neurobiology Instituto de Neurobiologia Universidad Nacional Autonoma de Mexico Campus Juriquilla Juriquilla Queretaro 76230 Mexico

Steroid hormones participate in the modulation of serotonergic transmission, including the regulation of synthetic and metabolic enzyme production, as well as receptor and transporter activity. The changes of 5-HT5A and 5-HT2c immunolabeling induced by steroids in the hippocampus ofovariectomized rats were studied in this work. Densitometric analysis in rat hippocampi were carried out for adjacent brain coronal immunolabeled sections after treatment with subcutaneous injections of vehicle, estradiol, progesterone or the combination of both steroids in ovariectomized rats. Exposure to estradiol and the combination of estradiol and progesterone significantly reduced the 5-HT5A-like immunosignal in the CA 1 region while progesterone did not induce changes. On the other hand, exposure to the combination of estradiol and progesterone or estradiol alone increased the 5-HT2c immunosignal in the same region. These results indicate that estradiol is involved in the discrete regulation of serotonin receptors 5-HT5A and 5-HT2c in rat hippocampus.

关键词： Serotonin receptor 5-HTsA 5-HT2c hippocampus estradiol progesterone

Prospective study of hepatitis B and D epidemiology and risk factors in Romania:A 10-year update

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World Journal of Hepatology 2024年第4期16卷 640-649页

作者： Speranta Iacob Liana Gheorghe Mirela Onica laura Huiban Corina Silvia Pop Ciprian Brisc Roxana Sirli Carmen Ester cristina Mihaela Brisc Sorina Diaconu Ion Rogoveanu Larisa Sandulescu Deiana Vuletici Anca Trifan Department of Gastroenterology Carol Davila University of Medicine and PharmacyBucharest 050474Romania Department of Gastroenterology and Hepatology Fundeni Clinical InstituteBucharest 022328Romania Department of Gastroenterology Grigore T Popa University of Medicine and PharmacyIasi 700115Romania Institute of Gastroenterology and Hepatology Saint Spiridon County HospitalIasi 700111Romania Department of Gastroenterology and Medical Oncology University Emergency Clinical HospitalBucharest 050098Romania Faculty of Medicine and Pharmacy University of OradeaOradea 410087Romania Department of Gastroenterology Emergency County HospitalOradea 410169Romania Center for Advanced Research in Gastroenterology and Hepatology Department of Gastroenterology and Hepatology“Victor Babeş”University of Medicine and PharmacyTimisoara 300041Romania Department of Gastroenterology and Hepatology TimişCounty Emergency Clinical Hospital"Pius Branzeu"Timisoara 300723Romania Department of Internal Medicine II and Gastroenterology University Emergency Clinical HospitalBucharest 050098Romania Department of Gastroenterology University of Medicine and PharmacyCraiova 200349Romania Department of Cardiology Emergency County HospitalCraiova 200642Romania Department of Gastroenterology Research Center of Gastroenterology and HepatologyUniversity of Medicine and PharmacyCraiova 200349Romania Department of Gastroenterology Emergency County HospitalCraiova 200642Romania Department of Gastroenterology Faculty of MedicineGrigore T Popa University of Medicine and PharmacyIasi 700115Romania

BACKGROUND The global burden of hepatitis D virus(HDV)infection represents a major medical challenge and a public health crisis ***,there is a lack of accurate data on the epidemiology and risk factors for *** B virus(HBV)and HDV coinfection causes the most severe form of viral hepatitis,leading to a higher cumulative incidence of liver-related events compared with HBV monoinfection,including the need for liver transplantation and *** To investigate the epidemiology,natural history,risk factors and clinical management of HBV and HDV coinfection in Romanian *** This prospective study was conducted between January and July 2022 in six tertiary gastroenterology and hepatology referral centres in *** consecutive adults admitted for any gastroenterology diagnosis who were HBV-positive were *** with acute hepatitis or incomplete data were *** the 25390 individuals who presented with any type of gastroenterology diagnosis during the study period,963 met the inclusion *** for anti-HDV antibodies and HDV RNA was performed for all *** and risk factor data were collected by investigators using medical charts and patient *** data were stored in an anonymized online database during the *** The prevalence of HBV was 3.8%;among these patients,the prevalence of HBV/HDV coinfection was 33.1%.The median age of the study population was 54.0 years,and it consisted of 55.1%men.A higher prevalence of HBV/HDV coinfection was observed in patients 50–69 years *** with HBV/HDV coinfection were significantly older than those with HBV monoinfection(P=0.03).Multivariate multiple regression analysis identified female gender(P=0.0006),imprisonment(P<0.0001),older age at diagnosis(P=0.01)and sexual contact with persons with known viral hepatitis(P=0.0003)as significant risk factors for *** This study shows that HDV infection among those with HBV re

关键词： Epidemiology Hepatitis B Hepatitis D Natural history Risk factors Romania

New neuroprotective perspectives in fighting oxidative stress and improving cellular energy metabolism by oleocanthal

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Neural Regeneration Research 2019年第7期14卷 1217-1218页

作者： cristina Angeloni laura Giusti Silvana Hrelia School of Pharmacy University of Camerino Department of Clinical and Experimental Medicine University of Pisa Department for Life Quality Studies Alma Mater Studiorum-University of Bologna

Neurodegenerative diseases are a heterogeneous group of disorders characterized by a progressive dysfunction and death of neural cells which lead to compromised motor or cognitive ***,the loss of neuron is associated with both gliosis and,frequently,with abnormal accumulation of extracellular and intracellular filamentous deposit in specific cell *** a rapidly increasing aging population,these diseases are becoming a primary health problem(Albers and Beal,2000).

关键词： New neuroprotective fighting oxidative

Potential roles of functional bacterial amyloid proteins,bacterial biosurfactants and other putative gut microbiota products in the etiopathogeny of Parkinson’s Disease

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BIOCELL 2021年第1期45卷 1-16页

作者： EMILIA MANOLE laura DUMITRESCU cristina NICULIȚE BOGDAN OVIDIU POPESCU laura cristina CEAFALAN Victor Babeș”National Institute of Pathology Bucharest050088Romania Colentina Clinical Hospital Bucharest020125Romania Carol Davila”University of Medicine and Pharmacy Bucharest020021Romania

An increasing number of studies provide evidence for the existence of a microbiota-gut-brain axis and its potential involvement in the development of sporadic Parkinson’s disease and other neurodegenerative *** neuropathologic hallmark of Parkinson’s disease is the presence of brain intraneuronal aggregates of misfolded alpha-synuclein,known as Lewy *** gut microbiota products may trigger alpha-synuclein conformational changes in the neurons of the enteric nervous system,which can then spread to the brain in a prion-like fashion through the vagus *** may interfere with neuroinflammatory pathways and susceptibility to *** this review,we assess the potential role of putative gut microbiota products in the etiopathogeny of Parkinson’s disease,with a special emphasis on functional bacterial amyloid proteins,bacterial biosurfactants,endotoxins and short-chain fatty *** possible roles of molecular hydrogen,a common byproduct of bacterial fermentation,are also addressed.

关键词： Alpha-synuclein Endotoxins Short-chain fatty acids

Evolutionary consequences of deception： Complexity and informational content of colony signature are favored by social parasitism

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Current Zoology 2014年第1期60卷 137-148页

作者： Maria cristina LORENZI laura AZZANI Anne-Genevieve BAGNRRES Department of Life Sciences and Systems Biology University of Turin via Accademia Albertina 13 10123 Torino Italy I.R.B.I. - UMR CNRS 7261 - Universit6 de Tours Facult6 des Sciences Parc Grandmont 37200 Tours France

Nestmate recognition codes show remarkable chemical complexity, involving multiple biochemical pathways. This complexity provides the opportunity to evaluate the ecological and social conditions that favor the evolution of complex signaling. We investigated how the chemical signatures of three populations of the social paper wasp Polistes biglumis differed in terms of concentration of hydrocarbons, proportions of branched hydrocarbons and overall variation. We tested whether the variation in chemical signatures among populations could be explained by the prevalence of social parasites or whether this was just an effect of local abiotic conditions which influenced the composition of the hydrocarbon cuticular layer. We studied the chemical signa- ture in three populations in which obligate social parasites differed in the selection pressures they imposed on host populations. Within each population, we restricted our analyses to non-parasitized hosts, to avoid potential short-term effects of parasite pres- ence on the host chemical signatures. We found that host colonies in parasitized populations had more diverse profiles than the parasite-free population. Moreover, the overall concentration of hydrocarbons and the relative proportion of branched hydrocar- bons were larger in the parasitized populations, relative to the non-parasitized one. This is to our knowledge the first evidence in favour of the hypothesis that different traits in the host chemical signatures as a whole undergo evolutionary changes resulting from directional or balancing selection imposed by social parasites. We conclude that obligate social parasites act as ＇engines of diversity＇ on host chemical signatures and operate in favor of a geographic mosaic of diverging communication codes

关键词： Polistes Brood parasitism Cipher Nestmate recognition Hydrocarbons Geographic mosaic Crozier's paradox

Methane oxidation and attenuation of sulphur compounds in landfill top cover systems: Lab-scale tests

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Journal of Environmental Sciences 2018年第3期30卷 317-326页

作者： Roberto Raga Alberto Pivato Maria cristina Lavagnolo laura Megido Raffaello Cossu DII-Department of Industrial Engineering University of Padova Department of Chemical and Environmental Engineering Polytechnic School of Engineering Gijón Campus University of Oviedo

In this study,a top cover system is investigated as a control for emissions during the aftercare of new landfills and for old landfills where biogas energy production might not be *** materials were studied as landfill cover system in lab-scale columns：mechanical–biological pretreated municipal solid waste（MBP）;mechanical–biological pretreated biowaste（PB）;fine（PBSf）and coarse（PBSc）mechanical–biological pretreated mixtures of biowaste and sewage sludge,and natural soil（NS）.The effectiveness of these materials in removing methane and sulphur compounds from a gas stream was tested,even coupled with activated carbon *** of CO2,CH4,O2,N2,H2S and mercaptans were analysed at different depths along the *** degradation was assessed using mass balance and the results were expressed in terms of methane oxidation rate（MOR）.The highest maximum and mean MOR were observed for MBP（17.2 g CH4/m^2/hr and 10.3 g CH4/m^2/hr,respectively）.Similar values were obtained with PB and *** lowest values of MOR were obtained for NS（6.7 g CH4/m^2/hr）and PBSf（3.6 g CH4/m^2/hr）,which may be due to their low organic content and void index,*** membranes with high load capacity did not seem to have an influence on the methane oxidation process：MBP coupled with 220 g/m^2and 360 g/m^2membranes gave maximum MOR of 16.5 g CH4/m^2/hr and 17.4 g CH4/m^2/hr,*** carbon membranes proved to be very effective on H2S ***,carbonyl sulphide,ethyl mercaptan and isopropyl mercaptan seemed to be easily absorbed by the filling materials.

关键词： Emission reduction Greenhouse gas emissionLandfill Methane oxidation H2S Soil cover

Murine models based on acute myeloid leukemia-initiating stem cells xenografting

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World Journal of Stem Cells 2018年第6期10卷 57-65页

作者： cristina Mambet Mihaela Chivu-Economescu Lilia Matei laura Georgiana Necula Denisa laura Dragu Coralia Bleotu Carmen cristina Diaconu Cellular and Molecular Pathology Department Stefan S. Nicolau Institute of Virology Nicolae Cajal Institute Titu Maiorescu University

Acute myeloid leukemia(AML) is an aggressive malignant disease defined by abnormal expansion of myeloid blasts. Despite recent advances in understanding AML pathogenesis and identifying their molecular subtypes based on somatic mutations, AML is still characterized by poor outcomes, with a 5-year survival rate of only 30%-40%, the majority of the patients dying due to AML relapse. Leukemia stem cells(LSC) are considered to be at the root of chemotherapeutic resistance and AML relapse. Although numerous studies have tried to better characterize LSCs in terms of surface and molecular markers, a specific marker of LSC has not been found, and still the most universally accepted phenotypic signature remains the surface antigens CD34+CD38- that is shared with normal hematopoietic stem cells. Animal models provides the means to investigate the factors responsible for leukemic transformation, the intrinsic differences between secondary post-myeloproliferative neoplasm AML and de novo AML, especially the signaling pathways involved in inflammation and hematopoiesis. However, AML proved to be one of the hematological malignancies that is difficult to engraft even in the most immunodeficient mice strains, and numerous ongoing attempts are focused to develop "humanized mice" that can support the engraftment of LSC. This present review is aiming to in-troduce the field of AML pathogenesis and the concept of LSC, to present the current knowledge on leukemic blasts surface markers and recent attempts to develop best AML animal models.

关键词： Acute myeloid leukemia Leukemia-initiating stem cells Antigen markers Murine models Xenografts

Benthic diatom community response to metal contamination from an abandoned Cu mine:Case study of the Gromolo Torrent(Italy)

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Journal of Environmental Sciences 2019年第1期31卷 233-246页

作者： Raffaella Tolotti Sirio Consani cristina Carbone Greta Vagge Marco Capello laura Cutroneo Department of Earth Environment and Life Sciences University of Genoa

Environmental contamination has become a global problem of increasing intensity due to the exponential growth of industrialization. One main debated issue is the metal contamination of rivers receiving Acid Mine Drainage(AMD) from active/abandoned mines. In order to assess the quality of lotic systems, diatoms are commonly used, as their assemblage modifies on the basis of changes in environmental parameters. Benthic diatom changes were analyzed along the metal-impacted Gromolo Torrent(Liguria, NW Italy) with the aim of understanding the effects of input from the abandoned Libiola Cu mine. The results support the hypothesis that metals from AMD lead to massive changes in diatoms, resulting in low biological diversity and in a shift of dominance, passing from the genera Cymbella and Cocconeis to more tolerant and opportunistic species, such as Achnanthidium minutissimum and Fragilaria rumpens. The high concentrations of labile metals, measured through Diffusion Gradients in Thin-films(DGT) immediately downstream of the two AMD inputs in the torrent, corresponded to a sudden decrease in the presence of diatoms, indicating the possible reaching of acute toxic levels. In particular,A. minutissimum dominated the mining area and was positively correlated with Cu and Zn;whereas F. rumpens bloomed downstream of this area, where the metal content was diluted,and was positively correlated with As and Pb. Finally, an important abundance of Nitzschia palea and teratological forms of A. minutissimum and F. rumpens were observed downstream from the mine, indicating that metals may have an important impact on diatoms up to the torrent mouth.

关键词： Benthic diatom community Biomarkers Metal contamination Diatom teratologies Acid Mine Drainage Libiola mine (Liguria,NW Italy)

Amine-rich carbon nitride nanoparticles:Synthesis,covalent functionalization with proteins and application in a fluorescence quenching assay

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Nano Research 2019年第8期12卷 1862-1870页

作者： Gabriele Capilli Simone Cavalera laura Anfossi cristina Giovannoli Marco Minella Claudio Baggiani Claudio Minero Department of Chemistry and NIS Center of Excellence University of Torino10125 TorinoItaly

Carbon nitride nanoparticles (CNNPs) have been employed as fluorescent sensing tools owing to their unique features, e.g. low cost production, high stability in water and high photoluminescence quantum yield. Here, an easy and versatile synthetic approach was exploited to design fluorescent nanoparticles with surface functionalities suitable for covalent binding to bioligands. High hydrophilic, brightly fluorescent CNNPs, rich of superficial amines, were obtained from the thermal condensation of urea and lysine (CNNP^Lys) and by tuning the precursor ratio and the heating time. Structure and size of the functionalized nanoparticles were characterized through infrared (IR) spectroscopy, transmission electron microscopy (TEM) and dynamic light scattering (DLS). Their optical properties were studied by ultraviolet-visible (UV-Vis) and fluorescence spectroscopy. The superficial primary amino groups, furnished by the lysine co-precursor, enabled for covalently linking CNNP^Lys to model proteins. The CNNP^Lys-protein conjugates excited under UV irradiation emit in the 400-450 nm visible range (quantum yield 24%). The applicability of CNNP^Lys as novel fluorescent probes was demonstrated by a fluorescence quenching assay, in which gold nanoparticles (GNPs) were attached to Staphylococcal protein A and employed to quench the CNNP^Lys fluorescence by Forster resonant energy transfer (FRET). The quenching occurred upon formation of the specific binding between the GNP-linked protein A and CNNP^Lys-tagged immunoglobulins, while the inhibition of the binding resulted in the recovery of CNNP^Lys luminescence. The synthetic strategy, based on combining a "conjugated polymer"-forming unit (urea) and a co-precursor able to provide the desired functional group (lysine), allows designing innovative materials for the development of new generation fluorescence biosensors in which easily functionalized fluorophores are needed.

关键词： fluorescent probe carbon nitride nanoparticles quenching assay fluorescent materials g-C3N4 Forster resonant energy transfer (FRET)