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DNA fragmentation in two cytometric sperm populations： relationship with clinical and ultrasound characteristics of the male genital tract

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Asian Journal of Andrology 2017年第3期19卷 272-279页

作者： Francesco Lotti lara tamburrino Sara Marchiani Elisa Maseroli Pasquale Vitale Gianni Forti Monica Muratori Mario Maggi Elisabetta Baldi Sexual Medicine and Andrology Unit Department of Experimental and Clinical Biomedical Sciences Center of Excellence DeNothe University of FlorenceViale Pieraccini 6 1-50036 Florence Italy.

We investigated whether DNA fragmentation in two cytometric sperm populations （PIddimmer and PIbriehter） with different biological characteristics and clinical relevance is related to clinical and color-Doppler ultrasound （CDUS） parameters of the male genital tract. One hundred and sixty males of infertile couples without genetic abnormalities were evaluated for clinical, scrotal, and transrectal CDUS characteristics, presence of prostatitis-like symptoms （with the National Institutes of Health-Chronic Prostatitis Symptom Index） and sperm DNA fragmentation （sDF） in PIdimmer and PIbrighter populations （using TUNEIJPI method coupled with flow cytometry）. Data were adjusted for age （Model 1） along with waistline, testosterone levels, smoking habit, and sexual abstinence （Model 2）. According to the statistical Model 2, PIdi sDF was associated with testicular abnormalities, including lower clinical and ultrasound volume （r = -0.21 and r = -0.20, respectively; P 〈 0.05）, higher FSH levels （r = 0.34, P 〈 0.0001） and occurrence of testicular inhomogeneity （P 〈 0.05） and hypoechogenicity （P 〈 0.05）. PIbrighter sDF was associated with prostate-related symptoms and abnormal signs, including higher NIH-CPSI total and subdomain scores, a higher prevalence of prostatitis-like symptoms and of CDUS alterations such as macro-calcifications, severe echo-texture inhomogeneity, hyperemia （all P 〈 0.05）, and higher arterial peak systolic velocity （r = 0.25, P 〈 0.05）. Our results suggest that DNA fragmentation in PIdimmer sperm, which is related to poor semen quality, mainly originates in the testicles, likely due to apoptosis. Conversely, DNA fragmentation in PIbrighter sperm appears to mainly originate during or after transit through the prostate, increasing with the presence of an inflammatory status of the organ. These results could lead to new perspectives for the identification of therapeutic targets to reduce sDF.

关键词： color-Doppler ultrasonography DNA fragmentation male infertility male sex organs spermatozoa

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Mechanisms and clinical correlates of sperm D damage

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Asian Journal of Andrology 2012年第1期14卷 24-31,174页

作者： lara tamburrino Sara Marchiani Margarita Montoya Francesco Elia Marino Ilaria Natali Marta Cambi Gianni Forti Elisabetta Baldi Monica Muratori Department of Clinical Physiopathology Andrology Unit and Center of Excellence ＇DeNothe＇ University of Florence 650139 Florence Italy

Among the different DNA anomalies that can be present in the male gamete, DNA fragmentation is the most frequent, particularly in infertile subjects. There is now consistent evidence that a sperm containing fragmented DNA can be alive, motile, morphologically normal and able to fertilize an oocyte. There is also evidence that the oocyte is able to repair DNA damage; however, the extent of this repair depends on the type of DNA damage present in the sperm, as well as on the quality of the oocyte. Thus, it is important to understand the possible consequences of sperm DNA fragmentation （SDF） for embryo development, implantation, pregnancy outcome and the health of progeny conceived, both naturally and by assisted reproductive technology （ART）. At present, data on the consequences of SDF for reproduction are scarce and, in many ways, inconsistent. The differences in study conclusions might result from the different methods used to detect SDF, the study design and the inclusion criteria. Consequently, it is difficult to decide whether SDF testing should be carried out in fertility assessment and ART. It is clear that there is an urgent need for the standardisation of the methods and for additional clinical studies on the impact of SDF on ART outcomes.

关键词： assisted reproduction COMET DNA fragmentation ICSI in vitrofertilization-embryo transfer sperm chromatin structure assay spermatozoa TUNEL

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New insights in sperm biology:How benchside results in the search for molecular markers may help understand male infertility

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World Journal of Translational Medicine 2016年第1期5卷 26-36页

作者： Sara Marchiani lara tamburrino Monica Muratori Elisabetta Baldi Department of Experimental and Clinical Biomedical Sciences University of Florence

The male factor is responsible for about 40% of couple infertility cases and such percentage is expected to increase in the future because of several likely factors including the presence of endocrine disruptors in the environment,changes in lifestyle habits and advanced couple *** such factors affect male fertility status,however,should be *** studies on male fertility status have focused on parameters analyzed using a spermiogram test,the primary diagnostic tool in the routine assessment of male infertility,which is,however,poorly predictive of both natural and medically assisted *** these reasons it is mandatory for the scientific community to identify new molecular markers to incorporate into the existing diagnostic tests of male ***,such markers would be detected in mature spermatozoa to avoid invasive procedures for the *** review summarizes the recent advancements in benchside approaches that appear most promising for the development of new diagnostic sperm fertility tests,or identification of therapeutic targets,and,illustrates their advantages and limits.

关键词： Sperm markers Male infertility Genetic and epigenetic approaches Proteomic approach Ion channels

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