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Pancreatic stellate cells promote tumor progression by promoting an immunosuppressive microenvironment in murine models of pancreatic cancer

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Pancreatic stellate cells promote tumor progression by promo...

The role of 2-arachidonylglyceryl in the regulation of the tumor microenvironment in murine models of pancreatic cancer

第十三届全国免疫学学术大会

作者： Caixia Li Lihua Cui Lei Yang Yuzhen Zhuo lanqiu zhang Shukun zhang Xiangfei Yuan Qi zhang Tianjin Nankai Hospital

Purpose: Pancreatic ductal adenocarcinoma(PDAC) is one of the most lethal forms of cancer with poor *** stellate cells(PSCs) play a vital role in PDAC *** aim of this study was to explore tumor microenvironment response to PSCs in an orthotopic pancreatic cancer mouse ***: To assess if PSCs secreted factors that can facilitate an immunosuppressive *** orthotopic tumor model, derived from co-injection of panc02 cells plus PSCs, was used to investigate tumor proliferation, metastasis and the population of immune cell in vivo, including regulatory T(Tregs), M2-type macrophages(M2), myeloid-derived suppressor cells(MDSCs), CD8 T cells, CD4 T cells, M1-type macrophages(M1), Natural killer(NK), and Natural killer T(NKT).Results: PSCs promoted PDAC growth not only induced cell proliferation and metastasis, but also significantly increased the suppressive immune cell population of Tregs, M2 and *** addition, PSCs decreased the immune cell population of CD8 T, CD4 T, M1, NK and NKT cells in the spleen and tumor tissues of the tumor-bearing ***: Our findings support PSCs playing multiple roles in PDAC development via promoting immunosuppressive microenvironment.

关键词： Pancreatic stellate cells pancreatic cancer myeloid-derived suppressor cells macrophages T cells

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The role of 2-arachidonylglyceryl in the regulation of the t...

第十三届全国免疫学学术大会

作者： Lei yang lanqiu zhang Xiangfei Yuan Caixia Li Lihua Cui Yuzhen Zhuo Shukun zhang Qi zhang Tianjin Nankai Hospital

Objective: 2-arachidonylglyceryl(2-AG) exhibits a direct antitumor effect in various tumor models. However, the immunomodulatory effect of 2-AG on PDAC microenvironment remains obscure. The aim of this study was to explore tumor microenvironment response to 2-AG in an orthotopic pancreatic cancer mouse ***: PDAC orthotopic tumor model was used to investigate tumor proliferation, autophagy and the population of immune cell in vivo, including dendritic cells(DCs), myeloid-derived suppressor cells(MDSCs), CD8+ T cells and CD4+ T cells. The effect of 2-AG on panc02 cell and DC2.4 cell were evaluated by flow cytometry. The protein levels of Atg5, LC3, and Belclin1 were measured by Western ***: 2-AG inhibited pancreatic cancer cell proliferation, which was blocked by AM251(a selective CB1 R antagonist) but not AM630(a selective CB2 R antagonist). In addition, 2-AG induced autophagic cell death by unregulating the expression of Atg5, LC3 and Belclin1. On the other hand, 2-AG induced DC phenotypic maturation, which was also blocked by AM251 but not AM630. However, did not affect the population of CD8+ T cells and CD4+ T cells in spleen and tumor microenvironment in murine models of pancreatic cancer. Moreover, we provide evidence that 2-AG administration induced the expansion of MDSCs in tumor bearing ***: Our findings support 2-AG playing multiple roles in PDAC development. 2-AG exhibits direct antitumor effects via inhibiting pancreatic cancer proliferation, inducing autophagic cell death and DC phenotypic, but also significantly promotes immunosuppressive microenvironment via increasing the suppressive immune cell population of MDSCs.

关键词： 2-arachidonylglyceryl pancreatic cancer myeloid-derived suppressor cells dendritic cells

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固有淋巴细胞在炎症中的作用研究进展

中华危重病急救医学 2017年第7期29卷 666-669页

作者：张兰秋王西墨天津市中西医结合急腹症研究所PI课题组天津市南开医院300100

固有淋巴细胞（ILCs）是在小鼠和人类体内新发现的一群淋巴细胞,是固有免疫的重要效应细胞,可调节适应性免疫反应.ILCs参与炎症的启动、调节和缓解过程,在小鼠机体感染、炎症和组织修复过程中发挥重要作用,临床研究也提示ILCs在人类的... 详细信息

固有淋巴细胞（ILCs）是在小鼠和人类体内新发现的一群淋巴细胞,是固有免疫的重要效应细胞,可调节适应性免疫反应.ILCs参与炎症的启动、调节和缓解过程,在小鼠机体感染、炎症和组织修复过程中发挥重要作用,临床研究也提示ILCs在人类的健康和疾病中扮演重要的角色.对相关文章进行回顾,旨在总结ILCs的种类、在急性和慢性炎症中的作用及其在缓解炎症和组织修复中的效应,为进一步研究提供方向.

关键词：固有淋巴细胞感染炎症组织修复

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组织成像质谱流式在消化系统肿瘤中的应用

中国免疫学杂志 2024年第4期40卷 866-871页

作者：刘泽涵荀敬张爱民王波涛张兰秋王西墨张琦天津医科大学研究生院天津300070 成都市第三人民医院暨西南交通大学附属医院普外科肝胆胰外科病区成都611700 天津市南开医院天津市急腹症器官损伤与中西医修复重点实验室天津300070 天津市南开医院检验科天津300070

组织成像质谱流式(IMC)是一种融合了质谱、高分辨激光消融、免疫组织/细胞化学的新技术。以独特的高维视角全面、精准地描绘组织和肿瘤微环境中复杂的表型、信号通路、肿瘤免疫的相互作用,在消化系统肿瘤中广泛应用。本文围绕IMC在描绘... 详细信息

组织成像质谱流式(IMC)是一种融合了质谱、高分辨激光消融、免疫组织/细胞化学的新技术。以独特的高维视角全面、精准地描绘组织和肿瘤微环境中复杂的表型、信号通路、肿瘤免疫的相互作用,在消化系统肿瘤中广泛应用。本文围绕IMC在描绘肿瘤微环境全景图、揭示肿瘤空间异质性、阐明肿瘤药理学机制、助力新药开发、动态评估免疫治疗疗效在消化系统肿瘤中应用展开综述。

关键词：组织成像质谱流式肿瘤微环境肿瘤异质性消化系统肿瘤免疫治疗

凉血活血方对脓毒症急性肾损伤小鼠肠道菌群及NLRP3/caspase-1/GSDMD焦亡通路的影响

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中华危重病急救医学 2023年第3期35卷 250-255页

作者：周旻杨磊卓玉珍李棣华张兰秋崔凌志李家瑞天津市南开医院肾内科天津300100 天津市南开医院中西医结合急腹症研究所天津300100 天津医科大学研究生院天津300070

目的:探讨凉血活血方对脓毒症急性肾损伤(AKI)小鼠肠道菌群、肠道屏障及肾组织NOD样受体蛋白3/天冬氨酸特异性半胱氨酸蛋白酶1/焦孔素D(NLRP3/caspase-1/GSDMD)细胞焦亡信号通路的影响。方法:将30只雄性C57BL/6小鼠随机分为假手术组(Sha... 详细信息

目的:探讨凉血活血方对脓毒症急性肾损伤(AKI)小鼠肠道菌群、肠道屏障及肾组织NOD样受体蛋白3/天冬氨酸特异性半胱氨酸蛋白酶1/焦孔素D(NLRP3/caspase-1/GSDMD)细胞焦亡信号通路的影响。方法:将30只雄性C57BL/6小鼠随机分为假手术组(Sham组)、脓毒症组(CLP组)、脓毒症+凉血活血方组(CLP+LXHX组),每组10只。通过盲肠结扎穿孔术(CLP)建立脓毒症AKI小鼠模型;Sham组仅进行开腹处理。制模前2 h,CLP+LXHX组给予凉血活血方水煎剂(6 g/kg)灌胃治疗;Sham组及CLP组给予等体积生理盐水灌胃。制模后24 h,麻醉处死小鼠,取结肠和肾组织及结肠内新鲜粪便。光镜下观察小鼠肾组织和结肠组织的病理变化;采用实时聚合酶链反应(RT-PCR)检测小鼠肾脏组织炎症因子白细胞介素(IL-1β和IL-18)的mRNA表达;采用蛋白质免疫印迹试验(Western blotting)检测小鼠肾组织中NLRP3、caspase-1和GSDMD的蛋白表达;采用16S rDNA高通量测序检测小鼠肠道菌群的变化。结果:与Sham组比较,CLP组肾脏组织炎症细胞浸润增加,肾脏空泡化,肾脏组织中IL-1β和IL-18的mRNA表达及NLRP3、caspase-1和GSDMD的蛋白表达均明显升高;结肠组织病理损伤显著,肠道菌群的物种丰富度显著降低,肠球菌和埃希菌-志贺菌的相对丰度显著增加,回肠杆菌属、拟普雷沃菌属、毛螺菌属、克雷伯菌属和副萨特菌属的相对丰度显著升高。与CLP组比较,凉血活血方可以显著减轻脓毒症小鼠肾脏组织和结肠组织病理损伤,并降低病理评分(分:肾脏组织为1.75±0.43比3.50±0.50,结肠组织为1.25±0.43比4.50±0.50,均 P<0.05),改善肠道菌群组成,降低肠球菌和埃希菌-志贺菌的相对丰度,并显著增加乳酸杆菌和阿克曼菌属的相对丰度,显著降低肾脏组织中IL-1β和IL-18的mRNA表达〔IL-1β mRNA(2 ^(-ΔΔCt)):1.59±0.05比4.61±0.88,IL-18 mRNA(2^(-ΔΔCt)):1.69±0.17比2.86±0.63,均 P<0.05〕及NLRP3、caspase-1和GSDMD的蛋白表达(NLRP3/GAPDH:0.71±0.04比0.89±0.01,caspase-1/GAPDH:1.04±0.04比1.48±0.04,GSDMD/GAPDH:0.90±0.01比1.41±0.02,均 P<0.05)。结论:凉血活血方对脓毒症所致AKI具有明显的保护作用,其作用机制可能通过调节肠道菌群改善肠道屏障,从而抑制肾脏组织中NLRP3/caspase-1/GSDMD信号通路的活化,降低焦亡相关炎症因子的表达。

关键词：脓毒症凉血活血方细胞焦亡肠道菌群急性肾损伤

Saclofen对铝抑制大鼠海马CA3区诱发电位的影响

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华中科技大学学报（医学版） 2007年第3期36卷 300-303页

作者：张兰秋肖鸿美赵勇李艾胡新武华中科技大学同济医学院基础医学院生理学系武汉430030

目的通过应用GABAB受体阻断剂Saclofen探讨金属铝对海马CA3区诱发电位的抑制作用是否与GAB-AB受体有关。方法应用单个电脉冲(波宽0.15ms,强度30V)刺激穿通纤维(PP),在海马CA3区记录诱发的群体峰电位(PS)。在CA3区微量注射AlCl3和Saclof... 详细信息

目的通过应用GABAB受体阻断剂Saclofen探讨金属铝对海马CA3区诱发电位的抑制作用是否与GAB-AB受体有关。方法应用单个电脉冲(波宽0.15ms,强度30V)刺激穿通纤维(PP),在海马CA3区记录诱发的群体峰电位(PS)。在CA3区微量注射AlCl3和Saclofen,观察它们对PS幅值的影响。结果①0.5mol/LAlCl3注入CA3区后,PS幅值较注药前显著减小(P<0.01或P<0.05)。②25μmol/L的Saclofen易化CA3区PS的幅值(P<0.01或P<0.05)。③50μmol/L Saclofen显著抑制CA3区PS的幅值(P<0.01或P<0.05)。④25μmol/L的Saclofen(P<0.05)和50μmol/L的Saclofen(P<0.01或P<0.05)均加强AlCl3的抑制效应。结论GABAB受体可能部分介导了铝对海马CA3区PS的抑制作用。

关键词：铝 GABAB受体 Saclofen 诱发电位海马

Method for Estimating the State of Health of Lithium-ion Batteries Based on Differential Thermal Voltammetry and Sparrow Search Algorithm-Elman Neural Network

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Energy Engineering 2025年第1期122卷 203-220页

作者： Yu zhang Daoyu zhang TiezhouWu School of Electrical and Electronic Engineering Hubei University of TechnologyWuhan430068China

Precisely estimating the state of health(SOH)of lithium-ion batteries is essential for battery management systems(BMS),as it plays a key role in ensuring the safe and reliable operation of battery ***,current SOH estimation methods often overlook the valuable temperature information that can effectively characterize battery aging during capacity ***,the Elman neural network,which is commonly employed for SOH estimation,exhibits several drawbacks,including slow training speed,a tendency to become trapped in local minima,and the initialization of weights and thresholds using pseudo-random numbers,leading to unstable model *** address these issues,this study addresses the challenge of precise and effective SOH detection by proposing a method for estimating the SOH of lithium-ion batteries based on differential thermal voltammetry(DTV)and an SSA-Elman neural ***,two health features(HFs)considering temperature factors and battery voltage are extracted fromthe differential thermal voltammetry curves and incremental capacity ***,the Sparrow Search Algorithm(SSA)is employed to optimize the initial weights and thresholds of the Elman neural network,forming the SSA-Elman neural network *** validate the performance,various neural networks,including the proposed SSA-Elman network,are tested using the Oxford battery aging *** experimental results demonstrate that the method developed in this study achieves superior accuracy and robustness,with a mean absolute error(MAE)of less than 0.9%and a rootmean square error(RMSE)below 1.4%.

关键词： Lithium-ion battery state of health differential thermal voltammetry Sparrow Search Algorithm

A Lightweight Multiscale Feature Fusion Network for Solar Cell Defect Detection

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Computers, Materials & Continua 2025年第1期82卷 521-542页

作者： Xiaoyun Chen Lanyao zhang Xiaoling Chen Yigang Cen Linna zhang Fugui zhang School of Mechanical Engineering Guizhou UniversityGuiyang550025China School of Computer Science and Technology Beijing Jiaotong UniversityBeijing100044China

Solar cell defect detection is crucial for quality inspection in photovoltaic power generation *** the production process,defect samples occur infrequently and exhibit random shapes and sizes,which makes it challenging to collect defective ***,the complex surface background of polysilicon cell wafers complicates the accurate identification and localization of defective *** paper proposes a novel Lightweight Multiscale Feature Fusion network(LMFF)to address these *** network comprises a feature extraction network,a multi-scale feature fusion module(MFF),and a segmentation ***,a feature extraction network is proposed to obtain multi-scale feature outputs,and a multi-scale feature fusion module(MFF)is used to fuse multi-scale feature information *** order to capture finer-grained multi-scale information from the fusion features,we propose a multi-scale attention module(MSA)in the segmentation network to enhance the network’s ability for small target ***,depthwise separable convolutions are introduced to construct depthwise separable residual blocks(DSR)to reduce the model’s parameter ***,to validate the proposed method’s defect segmentation and localization performance,we constructed three solar cell defect detection datasets:SolarCells,SolarCells-S,and *** and SolarCells-S are monocrystalline silicon datasets,and PVEL-S is a polycrystalline silicon *** results show that the IOU of our method on these three datasets can reach 68.5%,51.0%,and 92.7%,respectively,and the F1-Score can reach 81.3%,67.5%,and 96.2%,respectively,which surpasses other commonly usedmethods and verifies the effectiveness of our LMFF network.

关键词： Defect segmentation multi-scale feature fusion multi-scale attention depthwise separable residual block

Bilevel Optimal Scheduling of Island Integrated Energy System Considering Multifactor Pricing

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Energy Engineering 2025年第1期122卷 349-378页

作者： Xin zhang Mingming Yao Daiwen He Jihong zhang Peihong Yang Xiaoming zhang School of Automation and Electrical Engineering Inner Mongolia University of Science and TechnologyBaotou014010China

In this paper,a bilevel optimization model of an integrated energy operator(IEO)–load aggregator(LA)is constructed to address the coordinate optimization challenge of multiple stakeholder island integrated energy system(IIES).The upper level represents the integrated energy operator,and the lower level is the electricity-heatgas load *** to the benefit conflict between the upper and lower levels of the IIES,a dynamic pricing mechanism for coordinating the interests of the upper and lower levels is proposed,combined with factors such as the carbon emissions of the IIES,as well as the lower load interruption *** price of selling energy can be dynamically adjusted to the lower LA in the mechanism,according to the information on carbon emissions and load interruption *** benefits and win-win situations are achieved between the upper and lower ***,CPLEX is used to iteratively solve the bilevel optimization *** optimal solution is selected according to the joint optimal discrimination *** results indicate that the sourceload coordinate operation can reduce the upper and lower operation *** the proposed pricingmechanism,the carbon emissions and load interruption power of IEO-LA are reduced by 9.78%and 70.19%,respectively,and the capture power of the carbon capture equipment is improved by 36.24%.The validity of the proposed model and method is verified.

关键词： Bilevel optimal scheduling load aggregator integrated energy operator carbon emission dynamic pricing mechanism