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Chemical Research in Chinese Universities 2007年第3期23卷 319-324页

作者： ZHANG gui-rong GAO Ren-jun ZHANG Ai-jun RAO lang CAO Shu-gui Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education Jilin University Changchun 130021 P. R. China

To identify the desired hypertherrnophilic variants within a mutant esterase library for the resolution of （R, S）-2- octanol acetate, a simple, reliable, and versatile method was developed in this study. We built a screening strategy including two steps, first we selected agar plate with substrate to screen the enzymatic activity; secondly we used a pH indicator to screen the enantioselectivity. This method could rapidly detect favorable mutants with high activity and enantioselectivity. A total of 96. 2% of tedious screening work can be precluded using this screening strategy. It is an effective screening for alkyl ester and can be applied to relative screening researches. The four improved mutants were screened from the mutant esterase library. Their enantioselectivities, activities, and structures were investigated at different temperatures.

关键词： High-throughput screening Enantioselectivity Hyperthermophilic esterase Directed evolution

Fish kidney cells show higher tolerance to hyperosmolality than amphibian

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Aquaculture and Fisheries 2018年第3期3卷 135-138页

作者： lang gui Tao Li Qiya Zhang College of Fisheries and Life Science Shanghai Ocean UniversityShanghai 201306China State Key Laboratory of Freshwater Ecology and Biotechnology Institute of HydrobiologyChinese Academy of SciencesWuhan 430072China

In contrast to fish,amphibians inhabit both aquatic and terrestrial *** better understand osmoregulation in fish and amphibian,we have investigated the morphological changes in kidney cells to osmotic *** address this,kidney cell line isolated from the freshwater grass carp(CIK)and Chinese giant salamander(GSK)were challenged to different mediums with distinct osmotic pressures(100,300 and 700 mOsm).Morphological alterations of the fish and amphibian cells were compared by optical and electron *** hyposmotic treatment(100 mOsm),both CIK and GSK cells became unhealthy and show condensed chromatin,swollen mitochondria and cytoplasmic ***,after hyperosmotic treatment(700 mOsm),shrunken CIK cells with multipolar shape,pale or lightly stained cytoplasm,condensed chromatin,vacuoles and swollen mitochondria were *** cells were seriously damaged and most were completely *** results suggest that fish kidney cells show a higher degree of tolerance to hyperosmoticity by comparing to amphibians and provide novel insights on the osmoregulatory capacity and adaptability of kidney cells between the two animal groups.

关键词： kidney Morphological alterations

Recent insights into aquatic viruses: Emerging and reemerging pathogens, molecular features, biological effects, and novel investigative approaches

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Water Biology and Security 2022年第4期1卷 9-39页

作者： Qi-Ya Zhang Fei Ke lang gui Zhe Zhao Institute of Hydrobiology Chinese Academy of SciencesWuhan430072China Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources Ministry of EducationCollege of Fisheries and Life ScienceShanghai Ocean UniversityShanghai201306China Department of Marine Biology College of OceanographyHohai UniversityNanjing210098China

Aquatic viruses are naturally present in the aquatic environment and the number of viruses is *** multicellular organisms in aquatic ecosystems may be infected,cross-species transmitted,manipulated,and killed by aquatic viruses,which can lead to cascading ecological *** viruses in unicellular aquatic organisms can alter interactions between host individuals,and are essential in effecting or maintaining the dynamics of aquatic microbial communities,horizontal gene transfer,biodiversity,and modulating ecological processes ***,hosts also impact viral abundance and *** diversity drives multifunctionality in ecosystems,while viruses shape complex microbial communities and are crucial for ecosystem *** review focuses on molecular,genetic,evolutionary,and ecosystemic advances related to emerging and reemerging aquatic viruses,presents the contexts,novel tools,and investigative approaches pertaining to the study of aquatic virology,and discusses the mechanisms by which viruses affect aquatic *** paper provides an efficient and broadly-based blueprint for improving understanding of aquatic viruses.

关键词： Aquatic virus Organism killers Biological evolution engine Aquatic ecosystems Molecular features Different lineages Novel investigative approaches Biogeochemical cycles

Molecular basis of pathogenesis of emerging viruses infecting aquatic animals

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Aquaculture and Fisheries 2018年第1期3卷 1-5页

作者： lang gui V.Gregory Chinchar Qiya Zhang College of Fisheries and Ljfe Science Shanghai Ocean University.Shanghai 201306China Department of Microbiolbgy.University of Miss tsippi Medical Center 2500 North State StreetJacksonMS 39216USA State Key Laboratory of Freshwater Ecology and Botechnology Institute of Hydrobiology.Chinese Acodemy of SciencesWuhan 430072China

Aquatic vertebrates are very abundant in the world,and they are of tremendous importance in providing global food security and ***,emergent and resurgent viruses,such as ranavirus(e.g.,Rana grylio virus,RGV and Andriasd avidianus ranavirus,ADRV),herpesvirus(e.g.,Carassius carassius herpesvirus,CaHV),reovirus(e.g.,grass carp reovirus 109,GCRV-109,Scophthal musmaximus reovirus,SMReV and Micropterus salmoides reovirus,MsReV),and rhabdovirus(e.g.,Siniper cachuatsi rhabdovirus,SCRV and Scophthal musmaximus rhabdovirus,SMRV)can cause severe diseases in aquaculture animals and wild lower vertebrates,such as frogs,giant salamanders,fish,and so ***,we will briefly describe the symptoms produced by the aforementioned viruses and the molecular basis of the virus ehost *** manuscript aims to provide an overview of viral diseases in lower vertebrates with an emphasis on visible symptomatic manifestations and pathogenesis.

关键词： Ranavirus Herpesvirus Reovirus Rhabdovirus Aquatic animal

Characterization and expression analysis of sox3 in medaka gonads

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Aquaculture and Fisheries 2022年第1期7卷 23-30页

作者： Quan Pu Yuan Ma Ying Zhong Jing Guo lang gui Mingyou Li International Research Center for Marine Biosciences Ministry of Science and TechnologyShanghai Ocean UniversityShanghai201306China Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources Ministry of EducationShanghai Ocean UniversityShanghai201306China

Numerous studies have showed that sox3 is involved in neurogenesis and sex differentiation in ***,the accurate expression pattern is still unclear in *** this study,medaka sox3 was isolated and its expression patterns were compared with germ cell gene vasa in adult *** reverse transcription-polymerase chain reaction(RT-PCR)analysis,sox3 RNA expression was detected in the brain,eyes and *** chromogenic and fluorescent in situ hybridization,sox3 was present in the oogonia and early stages of oocytes as well as granulosa cells and theca cells in the later stages;in the testis,sox3 was occurred in the Sertoli cells and sperm with a specific signal in the location of chromatid body of sperm;in the embryos,sox3 was expressed in the central *** results suggest that medaka sox3 gene was occurred in somatic and germ cells in adult gonads of both sexes,involved in the process of spermatogenesis,as well as the development of the nervous *** study provides a precise expression pattern of sox3 and suggests that sox3 may be involved in gonadal and nervous system development of fish.

关键词： Medaka sox3 Gonad development Nervous system development

A droplet digital PCR method(ddPCR)for sensitive detection and quantification of Carassius auratus herpesvirus(CaHV)

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Water Biology and Security

Water Biology and Security 2024年第2期3卷 55-61页

作者： Yun Zhao Dan Xu Fei Ke Yan Zhou Mingyou Li lang gui Key Laboratory of Freshwater Aquatic Genetic Resources Ministry of Agriculture and Rural Affairs Shanghai Ocean UniversityShanghaiChina Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources Ministry of EducationShanghai Ocean UniversityShanghaiChina International Research Center for Marine Biosciences Ministry of Science and TechnologyShanghai Ocean UniversityShanghaiChina State Key Laboratory of Freshwater Ecology and Biotechnology Institute of HydrobiologyChinese Academy of SciencesWuhanChina

Carassius auratus herpesvirus(CaHV)is a pathogen isolated from crucian carp(Carassius auratus)associated with high mortality.A diagnosis method that can detect the virus at an early stage,specifically and accurately,is an urgent requirement for the prevention of CaHV *** the present study,a droplet digital PCR(ddPCR)method based on the tumor necrosis factor receptor(TNFR)gene was established to detect and quantify CaHV DNA with high specificity and no cross-reactions with other aquatic *** mucus samples were collected from infected crucian carp from Day 1–8 after infection,and positive amplification was detected on the first day by ddPCR(0.54 copies/μL),whereas the presence of CaHV was not detected by routine PCR until Day *** DNA was then collected from the head kidney of 20 fishes which were injected with CaHV and died during the *** five negative samples checked by routine PCR were detected by ddPCR and real-time PCR(qPCR),*** results showed that the positive detection rate of ddPCR(100%)was higher than that of qPCR(40%).The detection limit of the ddPCR was found to be 0.52 copies/μL,which was much lower than the 50.12 copies/μL determined by ***,ddPCR offers a highly promising diagnosis method for the absolute quantification of CaHV in carrier fish and samples from the skin mucus and head kidney with low viral concentrations.

关键词： Carassius auratus herpesvirus(CaHV) Tumor necrosis factor receptor(TNFR) Droplet digital PCR(ddPCR) Real-time PCR(qPCR) Diagnosis method

Medaka gcnf is a component of chromatoid body during spermiogenesis

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Aquaculture and Fisheries 2021年第6期6卷 574-582页

作者： Zinan Xie Peng Song Ying Zhong Jing Guo lang gui Mingyou Li International Research Center for Marine Biosciences Ministry of Science and TechnologyShanghai Ocean UniversityShanghai201306China Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources Ministry of EducationShanghai Ocean UniversityShanghai201306China

Germ cells of many organisms exhibit a unique perinuclear cytoplasmic cloud-like structure called the chromatoid body(CB).Specific components of the CB,such as vasa,are crucial for germline *** we identify another component of the CB,the Oryzias latipes gcnf homolog(Olgcnf).By reverse transcription polymerase chain reaction(RT-PCR),Olgcnf transcript was restricted to gonads in the adult tissues and was maternally provided and persisted throughout *** in situ hybridization on gonadal sections,Olgcnf transcript was restricted to germ *** the ovary,Olgcnf was predominantly expressed in the early stages of *** the testis,Olgcnf showed dynamic expression in round spermatids and sperm,accumulated in the CB during spermatid morphogenesis and concentrated in elongated spermatids of acrosome cap region and flagellar ***,Olgcnf was colocalized with vasa,which is the best-characterized component of the ***,Olgcnf is specific to germ cells of both sexes and is a component of the CB during spermiogenesis.

关键词： Gcnf Medaka Ovary Testis

Effect of individualized nutrition interventions on clinical outcomes of pregnant women with gestational diabetes mellitus

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World Journal of Diabetes 2023年第10期14卷 1524-1531页

作者： Jian-Ying Luo lang-gui Chen Mei Yan Yue-Jing Mei Ya-Qian Cui Min Jiang Department of Clinical Nutrition Northern Jiangsu People's Hospital of Jiangsu ProvinceNorthern Jiangsu People's Hospital Affiliated to Yangzhou UniversityYangzhou 225001Jiangsu ProvinceChina Clinical Medical College Yangzhou UniversityYangzhou 225009Jiangsu ProvinceChina Clinical Medical College Dalian Medical UniversityDalian 116000Liaoning ProvinceChina Department of Gynaecology and Obstetrics Northern Jiangsu People's Hospital of Jiangsu ProvinceNorthern Jiangsu People's Hospital Affiliated to Yangzhou UniversityYangzhou 225001Jiangsu ProvinceChina

BACKGROUND Gestational diabetes mellitus(GDM)can lead to excessive pregnancy weight gain(PWG),abnormal glucolipid metabolism,and delayed ***,it is necessary to provide appropriate and effective interventions for pregnant women with *** To clarify the effects of individualized nutrition interventions on PWG,glucolipid metabolism,and lactation in pregnant women with *** The study population consisted of 410 pregnant women with GDM who received treatment at the Northern Jiangsu People's Hospital of Jiangsu Provinceand Yangzhou Maternal and Child Health Hospital between December 2020 and December 2022,including 200 who received routine in-terventions[control(Con)group]and 210 who received individualized nutrition interventions[research(Res)group].Data on PWG,glucolipid metabolism[total cholesterol,(TC);triglycerides(TGs);fasting blood glucose(FPG);glycosylated hemoglobin(HbA1c)],lactation time,perinatal complications(cesarean section,premature rupture of membranes,postpartum hemorrhage,and pregnancy-induced hypertension),and neonatal adverse events(premature infants,fetal macrosomia,hypo-glycemia,and respiratory distress syndrome)were collected for comparative *** The data revealed markedly lower PWG in the Res group vs the Con group,as well as markedly reduced TG,TC,FPG and HbA1c levels after the intervention that were lower than those in the Con *** addition,obviously earlier lactation and statistically lower incidences of perinatal complications and neonatal adverse events were observed in the Res *** Individualized nutrition interventions can reduce PWG in pregnant women with GDM,improve their glucolipid metabolism,and promote early lactation,which deserves clinical promotion.

关键词： Individualized nutrition interventions Gestational diabetes mellitus Pregnancy weight gain Glycolipid metabolism Lactation time

Alagille syndrome associated with total anomalous pulmonary venous connection and severe xanthomas:A case report

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World Journal of Clinical Cases 2022年第25期10卷 8932-8938页

作者： Han-Shi Zeng Zhan-Hui Zhang Yan Hu gui-lang Zheng Jing Wang Jing-Wen Zhang Yu-Xiong Guo Department of Pediatrics Guangdong Provincial People’s HospitalGuangdong Academy of Medical SciencesGuangzhou 510030Guangdong ProvinceChina Department of Pediatrics Clinical Medicine Research InstituteThe First Affiliated HospitalJinan UniversityGuangzhou 510630Guangdong ProvinceChina

BACKGROUND Alagille syndrome(ALGS)is an autosomal dominant genetic disorder caused by mutations in the JAG1 or NOTCH2 *** is characterized by decreased intrahepatic bile ducts associated with a variety of abnormalities in many other organ systems,such as the cardiovascular,skeletal,and urinary *** SUMMARY We report a rare case of ALGS.A 1-month-old male infant presented with sustained jaundice and had a rare congenital heart disease:Total anomalous pulmonary venous connection(TAPVC).Sustained jaundice,particularly with cardiac murmur,caught our *** tests revealed elevated levels of alanine aminotransferase,aspartate aminotransferase,gamma-glutamyl transpeptidase,total bilirubin,and total bile acids,indicating serious intrahepatic *** confirmed the presence of butterfly vertebra at the seventh thoracic *** suggested ALGS,which was confirmed by genetic testing with a c.3197dupC mutation in the JAG1 *** was administered immediately after confirmation of the diagnosis,and cardiac surgery was performed when the patient was 1.5 month *** recovered well after treatment and was discharged at the age of 3 *** the age of two years,the patient returned to our clinic because multiple cutaneous nodules with xanthomas appeared,and their size and number increased over *** We report a unique case of ALGS associated with TAPVC and severe *** study has enriched the clinical manifestations of ALGS and emphasized the association between JAG1 gene and TAPVC.

关键词： Alagille syndrome JAG1 gene Notch signaling pathway Total anomalous pulmonary venous connection Severe xanthomas Case report

Characterization and expression analysis of gonad specific igf3 in the medaka ovary

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Aquaculture and Fisheries 2022年第3期7卷 259-268页

作者： Jiale Xie Ying Zhong Yuli Zhao Wenjie Xie Jing Guo lang gui Mingyou Li International Research Center for Marine Biosciences Ministry of Science and TechnologyShanghai Ocean UniversityChina Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources Ministry of EducationShanghai Ocean UniversityShanghai201306China

The gonad specific expression of insulin-like growth factor 3(igf3)has suggested an important role of igf3 in fish *** this study,medaka igf3 was isolated and its expression patterns were compared with igf1,and germ cell gene vasa in adult medaka *** cloning and sequencing showed that the open reading frame(ORF)of medaka igf3 was comprised of 501 nucleotides and encoded 166 amino acid *** transcription polymerase chain reaction(RT-PCR)analysis showed that igf3 RNA was specifically expressed in developing embryos and adult gonads of both *** time quantitative PCR(q-PCR)indicated that igf3 expression was gradually increased during oogenesis,and reached its highest level at stage *** Chromogenic and fluorescent in situ hybridization,igf3 was shown to be present in the germ cells of oogonia and in oocytes at stages I-III,as well as abundant in granulosa cells and theca cells of oocytes at stages *** addition,igf3 and igf1 were expressed mutually in the outer theca cells of stage V oocytes in ***,we demonstrated that gonad specific igf3 could mark medaka ovarian somatic cells and germ *** findings highlight the importance of igf3 during ovarian development.

关键词： igf3 igf1 Medaka Ovary