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中华泌尿外科杂志 2023年第1期44卷 66-67页

作者：蓝一笔尚丽明易楚繁张明津马春雷付伟金广西医科大学第一附属医院泌尿外科南宁530022 广西医科大学第一附属医院肝胆外科南宁530022

根治性肾切除术后对侧肾上腺及胆囊转移临床罕见, 本文报道1例。患者行腹腔镜根治性右肾切除术, 术后病理提示右肾透明细胞癌。术后5年复查CT检查提示左侧肾上腺及胆囊占位性病变, 行经腹入路腹腔镜左肾上腺肿瘤切除+胆囊切除术, 病理... 详细信息

根治性肾切除术后对侧肾上腺及胆囊转移临床罕见, 本文报道1例。患者行腹腔镜根治性右肾切除术, 术后病理提示右肾透明细胞癌。术后5年复查CT检查提示左侧肾上腺及胆囊占位性病变, 行经腹入路腹腔镜左肾上腺肿瘤切除+胆囊切除术, 病理检查提示左侧肾上腺和胆囊肿瘤符合透明细胞癌改变。术后患者口服靶向药物治疗, 术后随访10个月, 仍无瘤生存。

关键词：癌,肾细胞肾上腺胆囊转移

同种异体移植物炎性因子-1在糖尿病睾丸大鼠模型中的表达及意义

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实用医学杂志 2024年第1期40卷 65-71页

作者：李德朝张明津蓝一笔马春雷付伟金贺州市中医医院泌尿外科广西贺州542899 广西医科大学第一附属医院泌尿外科南宁530022

目的探讨同种异体移植物炎性因子-1(AIF-1)在糖尿病(DM)睾丸大鼠模型中的表达及其意义。方法糖尿病睾丸(DMT)大鼠模型,随机分为糖尿病睾丸4周组(DMT4W组)、糖尿病睾丸8周组(DMT8W)、糖尿病睾丸12周组(DMT12W)。正常对照组(NC组)分为正... 详细信息

目的探讨同种异体移植物炎性因子-1(AIF-1)在糖尿病(DM)睾丸大鼠模型中的表达及其意义。方法糖尿病睾丸(DMT)大鼠模型,随机分为糖尿病睾丸4周组(DMT4W组)、糖尿病睾丸8周组(DMT8W)、糖尿病睾丸12周组(DMT12W)。正常对照组(NC组)分为正常对照4周组(NC4W)、正常对照8周组(NC8W)、正常对照12周组(NC12W)。组织病理学检测各组睾丸形态学变化,免疫组织化学检测AIF-1蛋白表达,免疫荧光法观察AIF-1和NF-κB p65蛋白表达。结果组织病理学显示,与NC组相比,DMT组内睾丸内生精细胞数量,支持细胞、间质细胞、精子数明显减少。免疫组织化学结果显示,与NC组相比,DMT组睾丸内AIF-1蛋白表达显著增加(P<0.05)。免疫荧光结果显示,与NC组相比,DMT各组AIF-1和NF-κB p65蛋白荧光强度显著增强。结论DMT组织AIF-1蛋白过表达,提示其在DMT病变过程可能起重要作用,有望成为治疗新靶点和诊断标记物。

关键词：同种异体移植物炎性因子-1 糖尿病睾丸大鼠模型意义

Tuning the interfacial reaction environment via pH-dependent and induced ions to understand C–N bonds coupling performance in NO3- integrated CO2 reduction to carbon and nitrogen compounds over dual Cu-based N-doped carbon catalyst

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Journal of Energy Chemistry 2025年第1期100卷 273-285页

作者： Tianhang Zhou Chen Shen Zhenghao Wu Xingying lan Yi Xiao College of Carbon Neutrality Future Technology State Key Laboratory of Heavy Oil Processing China University of Petroleum (Beijing) Institute of Materials Science TU Darmstadt Department of Chemistry Xi'an Jiaotong-Liverpool University Department of Mechanical and Mechatronics Engineering and Waterloo Institute of Nanotechnology University of Waterloo Waterloo

Dual atomic catalysts （DAC）,particularly copper （Cu2）-based nitrogen （N） doped graphene,show great potential to effectively convert CO2and nitrate （NO3-） into important industrial chemicals such as ethylene,glycol,acetamide,and urea through an efficient catalytical process that involves C–C and C–N ***,the origin of the coupling activity remained unclear,which substantially hinders the rational design of Cu-based catalysts for the N-integrated CO2reduction reaction （CO2RR）.To address this challenge,this work performed advanced density functional theory calculations incorporating explicit solvation based on a Cu2-based N-doped carbon (Cu2N6C10) catalyst for *** calculations are aimed to gain insight into the reaction mechanisms for the synthesis of ethylene,acetamide,and urea via coupling in the interfacial reaction *** to the sluggishness of CO2,the formation of a solvation electric layer by anions （F-,Cl-,Br-,and I-） and cations (Na+,Mg2+,K+,and Ca2+) leads to electron transfer towards the Cu *** process significantly accelerates the reduction of *** results reveal that*CO intermediates play a pivotal role in N-integrated ***,the Cu2-based N-doped carbon catalyst examined in this study has demonstrated the most potential for C–N coupling to *** findings reveal that through the process of a condensation reaction between*CO and NH2OH for urea synthesis,*NO3-is reduced to*NH3,and*CO2to*CCO at dual Cu atom *** dual-site reduction facilitates the synthesis of acetamide through a nucleophilic reaction between NH3and the ketene ***,we found that the I-and Mg2+ions,influenced by pH,were highly effective for acetamide and ammonia synthesis,except when F-and Ca2+were ***,the mechanisms of C–N bond formation were investigated via ab-initio molecular dynamics simulations,and we found that adjusting the micro-environment can change the dominant side reaction,shift

关键词： pH-dependent micro-environments C–N coupling Dual Cu-based nitrogen carbon catalysts Explicit solvation model Ion stabilizer AIMD

LINC00839靶向调控miR-124-3p对膀胱癌细胞生物学行为的影响

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天津医药 2023年第5期51卷 464-468页

作者：吴潇芸吴林秀张丽娣蓝一笔张明津易楚繁付伟金广西卫生职业技术学院内科护理教研室 530023 广西医科大学第一附属医院泌尿外科

目的探讨LINC00839靶向调控miR-124-3p对膀胱癌细胞生物学行为的影响。方法实时荧光定量PCR(qPCR)检测膀胱癌和癌旁正常组织、膀胱癌细胞株(T24、5637、EJ)中LINC00839表达及抑制LINC00839后对miR-124-3p表达的影响。构建3条小干扰RNA-L... 详细信息

目的探讨LINC00839靶向调控miR-124-3p对膀胱癌细胞生物学行为的影响。方法实时荧光定量PCR(qPCR)检测膀胱癌和癌旁正常组织、膀胱癌细胞株(T24、5637、EJ)中LINC00839表达及抑制LINC00839后对miR-124-3p表达的影响。构建3条小干扰RNA-LINC00839(si-LINC00839-1组、si-LINC00839-2组和siLINC00839-3组)序列转染膀胱癌细胞。沉默LINC00839表达后,CCK-8检测LINC00839对细胞增殖的影响;Transwell检测LINC00839对细胞侵袭的影响;流式细胞术检测LINC00839对细胞凋亡的影响;双荧光素酶实验检测LINC00839与miR-124-3p的相互作用;免疫蛋白印迹法检测抑制LINC00839后对内皮素受体B(EDNRB)蛋白表达的影响。结果qPCR检测结果显示,与癌旁正常组织比较,膀胱癌组织中LINC00839表达增高(P<0.05);LINC00839在膀胱癌细胞5637表达最高。3条siRNA序列中,si-LINC00839-2抑制效率最高。与si-NC组相比,siLINC00839-2组细胞增殖率下降,侵袭数量减少,细胞凋亡率增加(P<0.05)。双荧光素酶报告检测结果显示,LINC00839与miR-124-3p之间存在互补结合位点。与si-NC组相比,si-LINC00839-2组可上调miR-124-3p表达,抑制EDNRB蛋白表达(P<0.05)。结论LINC00839可靶向调节miR-124-3p调控膀胱癌细胞增殖、侵袭和凋亡等生物学行为。

关键词：膀胱肿瘤细胞增殖细胞凋亡 LINC00839 miR-124-3p

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LINC00475在肾透明细胞癌中的表达及对肾癌细胞增殖和侵袭的影响

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临床泌尿外科杂志 2023年第10期38卷 785-790页

作者：赵建华郑盛锋张明津蓝一笔付伟金广西医科大学第一附属医院泌尿外科南宁530023 广西百色市田东县中医医院泌尿外科

目的:探讨LINC00475在肾透明细胞癌(clear cell renal cell carcinoma, ccRCC)组织中的表达及对肾癌细胞增殖和侵袭的影响及可能机制。方法:高通量转录组学测序筛选出ccRCC和癌旁正常肾组织差异表达长链非编码RNA。实时荧光定量PCR(q-P... 详细信息

目的:探讨LINC00475在肾透明细胞癌(clear cell renal cell carcinoma, ccRCC)组织中的表达及对肾癌细胞增殖和侵袭的影响及可能机制。方法:高通量转录组学测序筛选出ccRCC和癌旁正常肾组织差异表达长链非编码RNA。实时荧光定量PCR(q-PCR)检测20例ccRCC组织和癌旁正常肾组织、肾癌ACHN、caki-1、caki-2细胞中LINC00475表达。RNA干扰研究LINC00475的生物学功能,分别用CCK8和Transwell实验研究LINC00475对肾癌细胞增殖和侵袭的影响。免疫蛋白印迹法检测PYCR1 mRNA和蛋白表达。结果:与癌旁正常肾组织比较,LINC00475在ccRCC组织过表达(P<0.05);LINC00475在肾癌ACHN细胞中表达最高。沉默LINC00475表达后,抑制肾癌ACHN细胞增殖和侵袭(P<0.05),下调PYCR1 mRNA和蛋白表达(P<0.05)。结论:LINC00475过表达可能与肾癌的发生发展有关,沉默LINC00475可能调控PYCR1表达,从而抑制肾癌细胞增殖和侵袭等恶性生物学功能。

关键词： LINC00475 肾癌细胞增殖迁移

Laparoscopic liver resection utilizing the ventral avascular area of the inferior vena cava:A retrospective cohort study

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World Journal of Gastroenterology 2025年第1期31卷 42-57页

作者： Kun Huang Zhu Chen Heng Xiao Hai-Yang Hu Xing-Yu Chen Cheng-You Du Xiang lan Department of Hepatobiliary Surgery The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400000China Department of General Surgery Mianyang Hospital of Traditional Chinese MedicineMianyang 621000Sichuan ProvinceChina

BACKGROUND Laparoscopic liver resection(LLR)can be challenging due to the difficulty of establishing a retrohepatic tunnel under *** the third hepatic hilum before parenchymal transection often leads to significant liver mobilization,tumor compression,and bleeding from the short hepatic veins(SHVs).This study introduces a novel technique utilizing the ventral avascular area of the inferior vena cava(IVC),allowing SHVs to be addressed after parenchymal transection,thereby reducing surgical complexity and improving outcomes in in situ *** To introduce and evaluate a novel LLR technique using the ventral avascular area of the IVC and compare its short-term outcomes with conventional *** The clinical cohort data of patients with pathologically confirmed hepatocellular carcinoma or intrahepatic cholangiocarcinoma who underwent conventional LLR and novel LLR between July 2021 and July 2023 at the First Affiliated Hospital of Chongqing Medical University were retrospectively *** novel LLR,we initially separated the caudate lobe from the IVC using dissecting forceps along the ventral avascular area of the ***,we transected the parenchyma of the left and right caudate lobes from the caudal side to the cephalic side using the avascular area as a ***,we addressed the SHVs and finally dissected the root of the right hepatic vein or left hepatic *** short-term postoperative outcomes and oncological results of the two approaches were evaluated and *** A total of 256 patients were included,with 150(58.59%)undergoing conventional LLR and 106(41.41%)undergoing novel *** novel technique resulted in significantly larger tumor resections(6.47±2.96 cm vs 4.01±2.33 cm,P<0.001),shorter operative times(199.57±60.37 minutes vs 262.33±83.90 minutes,P<0.001),less intraoperative blood loss(206.92±37.09 mL vs 363.34±131.27 mL,P<0.001),and greater resection volume(345.11±31.40 mL vs 264.38±31.98 mL,P<0.001)comp

关键词： Laparoscopic liver resection Inferior vena cava Retrohepatic tunnel Short hepatic veins Complex liver tumors Intraoperative bleeding control

Gut microbiota-astrocyte axis: new insights into age-related cognitive decline

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Neural Regeneration Research 2025年第4期20卷 990-1008页

作者： lan Zhang Jingge Wei Xilei Liu Dai Li Xiaoqi Pang Fanglian Chen Hailong Cao Ping Lei Haihe Laboratory of Cell Ecosystem Department of GeriatricsTianjin Medical University General HospitalTianjinChina Tianjin Geriatrics Institute Tianjin Medical University General HospitalTianjinChina Department of Gastroenterology and Hepatology Tianjin Medical University General HospitalNational Key Clinical SpecialtyTianjin Institute of Digestive DiseasesTianjin Key Laboratory of Digestive DiseasesTianjinChina Department of Urology Tianjin Medical University General HospitalTianjinChina Tianjin Neurological Institution Tianjin Medical University General HospitalTianjinChina

With the rapidly aging human population,age-related cognitive decline and dementia are becoming increasingly prevalent *** is considered the main risk factor for cognitive decline and acts through alterations in the composition of the gut microbiota,microbial metabolites,and the functions of *** microbiota–gut–brain axis has been the focus of multiple studies and is closely associated with cognitive *** article provides a comprehensive review of the specific changes that occur in the composition of the gut microbiota and microbial metabolites in older individuals and discusses how the aging of astrocytes and reactive astrocytosis are closely related to age-related cognitive decline and neurodegenerative *** article also summarizes the gut microbiota components that affect astrocyte function,mainly through the vagus nerve,immune responses,circadian rhythms,and microbial ***,this article summarizes the mechanism by which the gut microbiota–astrocyte axis plays a role in Alzheimer’s and Parkinson’s *** findings have revealed the critical role of the microbiota–astrocyte axis in age-related cognitive decline,aiding in a deeper understanding of potential gut microbiome-based adjuvant therapy strategies for this condition.

关键词： age aging Alzheimer’s disease astrocytes cognitive decline dementia gut microbiota gut–brain axis microbial metabolites neuroinflammation Parkinson’s disease

Robust interface and excellent as-built mechanical properties of Ti–6Al–4V fabricated through laser-aided additive manufacturing with powder and wire

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 154-168页

作者： Fei Weng Guijun Bi Youxiang Chew Shang Sui Chaolin Tan Zhenglin Du Jinlong Su Fern lan Ng Key Laboratory for Liquid-Solid Structural Evolution and Processing of Materials Ministry of EducationShandong Engineering&Technology Research Center for Superhard MaterialSchool of Materials Science and EngineeringShandong UniversityJinan 250061China Institute of Intelligent Manufacturing Guangdong Academy of SciencesGuangzhou 510095China Singapore Institute of Manufacturing Technology 5 Cleantech Loop636732Singapore

The feasibility of manufacturing Ti-6Al-4V samples through a combination of laser-aided additive manufacturing with powder(LAAM_(p))and wire(LAAM_(w))was explored.A process study was first conducted to successfully circumvent defects in Ti-6Al-4V deposits for LAAM_(p) and LAAM_(w),*** the optimized process parameters,robust interfaces were achieved between powder/wire deposits and the forged substrate,as well as between powder and wire *** characterization results revealed the epitaxial prior β grains in the deposited Ti-6Al-4V,wherein the powder deposit was dominated by a finerα′microstructure and the wire deposit was characterized by lamellar α *** mechanisms of microstructure formation and correlation with mechanical behavior were analyzed and *** mechanical properties of the interfacial samples can meet the requirements of the relevant Aerospace Material Specifications(AMS 6932)even without post heat *** fracture occurred within the interfacial area,further suggesting the robust *** findings of this study highlighted the feasibility of combining LAAM_(p) and LAAM_(w) in the direct manufacturing of Ti-6Al-4V parts in accordance with the required dimensional resolution and deposition rate,together with sound strength and ductility balance in the as-built condition.

关键词： laser-aided additive manufacturing powder deposition wire deposition interfacial characteristic mechanical behavior

Small extracellular vesicles derived from human induced pluripotent stem cell-differentiated neural progenitor cells mitigate retinal ganglion cell degeneration in a mouse model of optic nerve injury

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Neural Regeneration Research 2025年第2期20卷 587-597页

作者： Tong Li Hui-Min Xing Hai-Dong Qian Qiao Gao Sheng-lan Xu Hua Ma Zai-Long Chi State Key Laboratory of Ophthalmology Optometry and Visual ScienceEye HospitalWenzhou Medical UniversityWenzhouZhejiang ProvinceChina National Clinical Research Center for Ocular Diseases Eye HospitalWenzhou Medical UniversityWenzhouZhejiang ProvinceChina

Several studies have found that transplantation of neural progenitor cells(NPCs)promotes the survival of injured ***,a poor integration rate and high risk of tumorigenicity after cell transplantation limits their clinical *** extracellular vesicles(sEVs)contain bioactive molecules for neuronal protection and *** studies have shown that stem/progenitor cell-derived sEVs can promote neuronal survival and recovery of neurological function in neurodegenerative eye diseases and other eye *** this study,we intravitreally transplanted sEVs derived from human induced pluripotent stem cells(hiPSCs)and hiPSCs-differentiated NPCs(hiPSC-NPC)in a mouse model of optic nerve *** results show that these intravitreally injected sEVs were ingested by retinal cells,especially those localized in the ganglion cell *** with hiPSC-NPC-derived sEVs mitigated optic nerve crush-induced retinal ganglion cell degeneration,and regulated the retinal microenvironment by inhibiting excessive activation of *** analysis further revealed that hiPSC-NPC derived sEVs transported neuroprotective and anti-inflammatory miRNA cargos to target cells,which had protective effects on RGCs after optic nerve *** findings suggest that sEVs derived from hiPSC-NPC are a promising cell-free therapeutic strategy for optic neuropathy.

关键词： exosome miRNA neural progenitor cell neurodegeneration neuroinflammation neuroprotection optic nerve crush optic neuropathy retinal ganglion cell small extracellular vesicles