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Isolation of bioactive antioxidant compounds from the aerial parts of Allium roseum *** *** Regel.

Journal of Coastal Life Medicine 2016年第4期4卷 305-309页

作者： lamia sakka rouis-soussi Asma El Ayeb Saoussen Ben Salem Hichem Ben Jannet Fethia Harzallah-Skhiri Laboratory of Genetic Biodiversity and Valorisation of BioresourcesLR11ES41Higher Institute of Biotechnology of MonastirUniversity of MonastirMonastir 5000Tunisia Laboratory of Heterocyclic Chemistry Natural Products and ReactivityLR11SE39Team:Medicinal Chemistry and Natural ProductsFaculty of Science of MonastirUniversity of MonastirMonastir 5000Tunisia

Objective:To assess the antioxidant activity of aerial parts of Allium roseum *** *** Regel.(*** *** *** Regel.)for the first time,as well as to isolate the main bioactive ***:The chloroformic extract of Allium roseum(***)and their fractions obtained by subjection to a chromatographic study were tested for their antioxidant activities by using 2,2-diphenyl-2-picrylhydrazyl and 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)*** activity-guided purification was conducted to isolate five compounds in pure form where their structures were identified by means of nuclear magnetic resonance analyses,including 1D and 2D nuclear magnetic resonance ***:The evaluation of the antioxidant activity of chloroformic extract and their fractions from *** *** *** *** interesting *** active chloroformic extract afforded five isolated compounds where their structures were identified as β-sitosterol(1),chrysoeriol(2),luteolin(3),apigenin(4),andβ-sitosterol 3-O-β-D-glucoside(5).All the compounds were isolated for the first time from the *** *** *** *** three flavonoids(2–4)exhibited the highest antioxidant activity with IC_(50) values of 62.28,21.26 and 513.42μg/mL,respectively(2,2-diphenyl-2-picrylhydrazyl assay)and 218.00,73.50 and 877.66μg/mL,respectively[2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)assay].An important value of trolox equivalent antioxidant capacity(2.10 mmol/L)was reported for luteolin(3).Conclusions:These results may suggest that the *** *** *** *** great potential as a source of a natural preservative ingredient in beneficial for natural health products.

关键词： Allium roseum var.grandiflorum subvar.typicum Regel. Bio-guided isolation Antioxidant activity Flavonoids

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