Isolation of bioactive antioxidant compounds from the aerial parts of Allium roseum *** *** Regel.

作     者：Lamia Sakka Rouis-Soussi Asma El Ayeb Saoussen Ben Salem Hichem Ben Jannet Fethia Harzallah-Skhiri 

作者机构：Laboratory of GeneticBiodiversity and Valorisation of BioresourcesLR11ES41Higher Institute of Biotechnology of MonastirUniversity of MonastirMonastir 5000Tunisia Laboratory of Heterocyclic ChemistryNatural Products and ReactivityLR11SE39Team:Medicinal Chemistry and Natural ProductsFaculty of Science of MonastirUniversity of MonastirMonastir 5000Tunisia 

出 版 物：《Journal of Coastal Life Medicine》 (海岸生命医学杂志（英文版）)

年 卷 期：2016年第4卷第4期

页      面：305-309页

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Supported by the Ministry of High Education and Scientific Research MHSSR of Tunisia(Grant No.11/TM06) 

主　　题：Allium roseum var.grandiflorum subvar.typicum Regel. Bio-guided isolation Antioxidant activity Flavonoids 

摘      要：Objective:To assess the antioxidant activity of aerial parts of Allium roseum *** *** Regel.(*** *** *** Regel.)for the first time,as well as to isolate the main bioactive ***:The chloroformic extract of Allium roseum(***)and their fractions obtained by subjection to a chromatographic study were tested for their antioxidant activities by using 2,2-diphenyl-2-picrylhydrazyl and 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)*** activity-guided purification was conducted to isolate five compounds in pure form where their structures were identified by means of nuclear magnetic resonance analyses,including 1D and 2D nuclear magnetic resonance ***:The evaluation of the antioxidant activity of chloroformic extract and their fractions from *** *** *** *** interesting *** active chloroformic extract afforded five isolated compounds where their structures were identified as β-sitosterol(1),chrysoeriol(2),luteolin(3),apigenin(4),andβ-sitosterol 3-O-β-D-glucoside(5).All the compounds were isolated for the first time from the *** *** *** *** three flavonoids(2–4)exhibited the highest antioxidant activity with IC_(50) values of 62.28,21.26 and 513.42μg/mL,respectively(2,2-diphenyl-2-picrylhydrazyl assay)and 218.00,73.50 and 877.66μg/mL,respectively[2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid)assay].An important value of trolox equivalent antioxidant capacity(2.10 mmol/L)was reported for luteolin(3).Conclusions:These results may suggest that the *** *** *** *** great potential as a source of a natural preservative ingredient in beneficial for natural health products.