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Human physiome based on the high-resolution dataset of human body structure

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Progress in Natural Science:Materials International 2008年第8期18卷 921-925页

作者： Qian Liu, Bo Wu, Shaoqun Zeng, qingming luo * Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, China Britton Chance Center for Biomedical Photonics Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology Wuhan 430074 China

Physiome Project, as a new concept, is proceeding rapidly with the great advancement of genomics, physiological experiment, biologic modeling and computer simulation technique. The project seeks to provide a quantitative framework for modeling of the human physiological system using computational approaches, which is able to integrate the knowledge of molecular biology, biochemical, biophysical and anatomical information on different levels, including cell, tissue, organ, system and organism. This paper reviews the development of the Physiome Project in the past decade. The role of high-resolution datasets of human body structure in Physiome Project is discussed. The future plan and applications of the high-resolution datasets of human body structure to Physiome Project are discussed as well.

关键词： Physiome Physiome Project Multi-scale modeling Anatomical modeling

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混浊介质中后向单次漫散射米勒矩阵特征

光学学报 2006年第3期26卷 419-424页

作者：邓勇骆清铭鲁强华中科技大学生物医学光子学教育部重点实验室

采用斯托克斯(Stokes)矢量形式,推导出当无限窄的连续光束垂直入射到混浊介质表面时,后向单次漫散射米勒(Mueller)矩阵的解析表达式。基于米氏(Mie)散射模式,详细分析了单次散射米勒矩阵元素的分布模式,以及与介质粒子数密度,粒子尺寸... 详细信息

采用斯托克斯(Stokes)矢量形式,推导出当无限窄的连续光束垂直入射到混浊介质表面时,后向单次漫散射米勒(Mueller)矩阵的解析表达式。基于米氏(Mie)散射模式,详细分析了单次散射米勒矩阵元素的分布模式,以及与介质粒子数密度,粒子尺寸参量之间的关系。研究表明:单次散射米勒矩阵的方位变化随粒子数密度的增加,逐渐消失,而矩阵元素m22,m33,m23,m32随粒子数密度的变化,具有更显著的方位变化特征。矩阵元素m22,m33在方位角=45°时的值随尺寸参量的变化有一定的规律性,当尺寸参量小于某一特征参量时,其值呈下降趋势,反之则呈波动上升趋势。当介质粒子数密度以及粒子尺寸参量改变时,米勒矩阵元素强度的径向分布模式不变,即在任何方位,强度随径向距离都近似成指数规律衰减,方位变化呈周期性。

关键词：医用光学与生物技术漫散射米勒矩阵偏振混浊介质

用基于方位分辨的漫后向散射光确定粒子尺寸分布及相对折射率

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光学学报 2006年第8期26卷 1214-1219页

作者：邓勇鲁强骆清铭华中科技大学生物医学光子学教育部重点实验室武汉光电国家实验室生物医学光子学研究部武汉430074

基于偏振门技术,提出了利用漫后向散射光随线偏振光入射方位的变化来测量双层模型表层的粒子尺寸分布及相对折射率。双层物理模型用来模拟上皮组织,上层分别由聚苯乙烯小球或HeLa细胞悬浮液组成,下层由脂肪乳溶液组成,每层的光学特性与... 详细信息

基于偏振门技术,提出了利用漫后向散射光随线偏振光入射方位的变化来测量双层模型表层的粒子尺寸分布及相对折射率。双层物理模型用来模拟上皮组织,上层分别由聚苯乙烯小球或HeLa细胞悬浮液组成,下层由脂肪乳溶液组成,每层的光学特性与上皮组织光学特性匹配。根据米氏(Mie)散射理论,建立了与散射体形态参量相关的偏振后向漫散射强度随入射偏振方位变化的理论模型,并采用浮点遗传算法反演程序获得表层粒子的尺寸分布(PSD)及相对折射率。将理论值和实验测量值进行比较。研究结果表明通过测量随方位变化的后向漫散射光的偏振差分信号,能够获得类上皮组织模型表层的形态结构信息。

关键词：医用光学与生物技术尺寸分布及折射率偏振光双层模型

用遗传算法反演类上皮组织模型的偏振散射光谱

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光学学报 2006年第9期26卷 1377-1381页

作者：邓勇鲁强骆清铭华中科技大学生物医学光子学教育部重点实验室武汉光电国家实验室武汉430074

基于生物组织的粒状模型以及光谱对米氏(Mie)散射体形态的灵敏性,构建了类上皮组织模型的偏振后向散射光谱反演模型。针对反演模型的多参量、多极值、非线性,涉及到非常复杂的三角函数计算,模型空间极小的特点,采用每代保留最优个体的... 详细信息

基于生物组织的粒状模型以及光谱对米氏(Mie)散射体形态的灵敏性,构建了类上皮组织模型的偏振后向散射光谱反演模型。针对反演模型的多参量、多极值、非线性,涉及到非常复杂的三角函数计算,模型空间极小的特点,采用每代保留最优个体的浮点遗传算法对类上皮组织模型的偏振后向散射光谱反演。对编码策略、适值调整策略及选择策略进行了讨论。研究结果表明:经过70代反演迭代,每个参量的相对误差趋于稳定,最小的达到0.02%左右,最大的不超过3%。采用基于实数编码的遗传算法能从偏振散射光谱中同时反演获得表层粒子的形态参量,具有全局收敛性和良好的反演精度与抗噪声能力。

关键词：医用光学与生物技术偏振光散射光谱遗传算法类上皮组织模型

High-resolution photoacoustic microscope for rat brain imaging in vivo

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Chinese Optics Letters 2010年第6期8卷 609-611页

作者：杨孝全蔡鑫 Konstantin Maslov 汪立宏骆清铭 Britton Chance Center for Biomedical Photonics Wuhan National Laboratory for OptoelectronicsHuazhong University of Science and Technology Optical Imaging Laboratory Department of Biomedical EngineeringWashington University in Saint LouisSaint Louis63130-4899USA

A reflection-mode photoacoustic microscope（PAM） for rat brain imaging in vivo is constructed.A pulsed laser is used as an excitation source,and a focused ultrasound transducer is adopted to collect the photoacoustic *** scanning is applied to acquire three-dimensional（3D） *** obtained measurements of the lateral and axial resolutions of the microscope are 45 and 15μm,*** imaging depth in the chicken breast tissue is 3.1 mm at a signal-to-noise ratio（SNR） of 20 dB without any signal *** imaging speed is 30 A-line/*** results in vivo demonstrate the capability of 3D imaging of the brain vessels of the rat after removing the skull.

关键词： Acoustic microscopes Laser excitation Photoacoustic effect Photoacoustic microscopy Pulsed laser applications Pulsed lasers Signal to noise ratio Ultrasonic transducers

Line-scanning microscopy with laterally symmetric imaging using simultaneous cross-line illumination

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Photonics Research 2024年第7期12卷 1513-1521页

作者： DAN SHEN YAFENG LI MENG WANG YUTONG HAN BOLIN LU HUI GONG qingming luo JING YUAN Britton Chance Center for Biomedical Photonics and MoE Key Laboratory for Biomedical Photonics Wuhan National Laboratory for OptoelectronicsHuazhong University of Science and TechnologyWuhan 430074China HUST-Suzhou Institute for Brainsmatics JITRI Institute for BrainsmaticsSuzhou 215123China School of Biomedical Engineering Hainan UniversityHaikou 570228China

Using an on-the-fly scanning scheme, line confocal microscopy can obtain complex structures of large biological tissues with high throughput. Yet, it suffers from lateral imaging asymmetry and thus introduces the potential deformations of the observation results. Here, we propose cross-line illumination microscopy (cLIM) that acquires the imaging data of two perpendicular directions simultaneously through the same objective lens in a line scanning and utilizes two-direction deconvolution fusion to achieve lateral symmetric imaging *** fluorescence beads indicates that cLIM reduces lateral resolution asymmetry from 46.1%to 2.5%and improves lateral resolution by 31.0%, compared with traditional line-scanning imaging. Compared with commercial point-confocal microscopy, the cLIM has a 25.84×increase in imaging speed and 1.93×better background-suppressing ability when imaging an 11,306μm×7783μm×100μm mouse kidney slice. We also show the advantages of the cLIM in observing direction-sensitive texture features by imaging a muscular tissue slice. cLIM offers a novel solution to achieve laterally symmetric line-scanning imaging with simple modifications while maintaining high throughput and accuracy for imaging large-scale samples.

关键词： illumination simultaneous scanning

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双层散射介质的单次后向散射光谱分析

光学学报 2006年第4期26卷 595-599页

作者：邓勇骆清铭鲁强华中科技大学生物医学光子学教育部重点实验室武汉光电国家实验室生物医学光子学研究部武汉430074

从上皮组织的结构特点出发,基于米氏(Mie)散射理论,建立了双层散射介质的单次后向散射光谱的理论模型,该模型通过偏振门屏蔽来自下层的噪声背景,只保留来自上层的单次散射光。计算分析了粒子的形态学参量如平均尺寸及其分布、相对折射... 详细信息

从上皮组织的结构特点出发,基于米氏(Mie)散射理论,建立了双层散射介质的单次后向散射光谱的理论模型,该模型通过偏振门屏蔽来自下层的噪声背景,只保留来自上层的单次散射光。计算分析了粒子的形态学参量如平均尺寸及其分布、相对折射率变化时,单次散射光谱的特征。并用傅里叶波形分析法研究了这些参量对单次后向散射光谱曲线形状及其谐波幅值的影响。结果表明,这些只经历了表层粒子单次散射的光谱信号对表层粒子的平均尺寸及其分布、相对折射率具有灵敏性。对光谱曲线波纹结构的幅值、频率,散射强度,光谱谐波的幅值有直接的影响。研究结果对早期癌症的散射光谱特征识别及其特征提取有重要的实用价值。

关键词：医用光学与生物技术散射光谱米氏理论散射介质

Firing patterns of long-term cultured neuronal network on multi-electrode array

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Progress in Natural Science:Materials International 2006年第12期16卷 1337-1342页

作者： LI Xiangning, ZHOU Wei, LIU Man, ZENG Shaoqun and luo qingming**(The Key Laboratory of Biomedical Photonics of Ministry of Education-Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, China) The Key Laboratory of Biomedical Photonics of Ministry of Education-Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology Wuhan 430074 China

Spontaneous neuronal activity plays an important role in the development and plasticity of brain. To explore the developmental changes in the firing pattern of the neuronal networks in vitro, the hippocampal neurons were cultured on the multi-microelectrode array dish for over 14 weeks and the spontaneous activity was recorded. The results showed that random firing was observed in the 1st week and transformed into synchronized activity after two weeks, then tightly synchronized activity appeared in week 2 to 7 and finally the activities transformed into the random firing pattern. These results suggested three stages in the long-term development of neuronal network in vitro: the stage for connection, the stage of synchronized activity and the mature stage. Synchronized firing shown by spontaneous activity was an important phenomenon in high density cultured neuronal network and transformed patterns during development.

关键词： neuronal networks development multi-electrode array cultured neuron.

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一种简便的计算层析系统X射线硬化校正方法

光学学报 2007年第2期27卷 302-306页

作者：方正孙小敏骆清铭华中科技大学生物医学光子学教育部重点实验室武汉光电国家实验室(筹)生物医学光子学研究部武汉430074

以实物拍摄为依据,用一种最简便的修正方法解决计算机X射线层析术成像时由于硬化效应引起的切片图灰度失真问题。用高性能数字X射线机FAXITRON MX-20(射线管焦点20μm,探测板灰度等级16位)对不同厚度的物体进行透射成像,测得对应的透射... 详细信息

以实物拍摄为依据,用一种最简便的修正方法解决计算机X射线层析术成像时由于硬化效应引起的切片图灰度失真问题。用高性能数字X射线机FAXITRON MX-20(射线管焦点20μm,探测板灰度等级16位)对不同厚度的物体进行透射成像,测得对应的透射光强度,并利用新创的指数拟合法得到理想的拟合曲线,由此推导硬化效应的指数校正公式;最后利用实验室的微型计算机层析设备进行扇形束扫描,并逆投影重建生成计算层析断层图像,验证了该校正方法的实用性。该指数拟合法的误差不到常用的二阶多项式拟合法的1/3,对物体计算层析重构,硬化校正以前有明显的“杯状”伪迹,切片灰度不均匀,用指数法修正以后该伪迹消失,切片灰度均匀。

关键词：医用光学与生物技术计算层析硬化效应曲线拟合 “杯状”伪迹滤波逆投影