检索结果-南通市图书馆

中低强度运动干预高脂饲养小鼠膝关节损伤软骨细胞LncRNA HOTAIR的表达

在线全文

学校读者我要写书评

暂无评论

中国组织工程研究 2024年第11期28卷 1684-1689页

作者：张慧珍吴伟罗海涛甘肃省体育科学研究所甘肃省兰州市730050 上海体育学院上海市200438

背景:中低强度有氧运动对膝关节软骨具有一定保护作用。长链非编码RNA对基因调节具有重要作用,其中,HOTAIR可以动员多个转录共同抑制因子,抑制某些基因表达。目的:检测HOTAIR及相关因子在高脂饲养小鼠及中低强度运动干预后膝关节软骨细... 详细信息

背景:中低强度有氧运动对膝关节软骨具有一定保护作用。长链非编码RNA对基因调节具有重要作用,其中,HOTAIR可以动员多个转录共同抑制因子,抑制某些基因表达。目的:检测HOTAIR及相关因子在高脂饲养小鼠及中低强度运动干预后膝关节软骨细胞中表达水平,探讨其在软骨损伤及运动康复中的作用。方法:C57BL/6雄性小鼠30只,随机分为对照组(正常饲养)、高脂静养组和高脂运动组,每组10只。高脂运动组进行8周中低强度跑台运动,对照组和高脂静养组笼中饲养,期间每周日称体质量。8周跑台结束后取材双下肢,采用Micro CT扫描膝关节,股骨髁成3D图像,获取相关参数;膝关节苏木精-伊红和番红固绿染色进行关节Mankin和OARSI评分;RT-PCR检测相关软骨代谢指标Ⅱ型胶原、基质金属蛋白酶13、白细胞介素1,脂代谢指标低密度脂蛋白受体相关蛋白5(LRP5),HOTAIR及其下游因子赖氨酸特异性去甲基化酶1(LSD1)mRNA表达。结果与结论:①高脂静养组和高脂运动组体质量均高于对照组(P<0.01),高脂运动组体质量低于高脂静养组(P<0.05);②高脂静养组和高脂运动组骨小梁连接密度均高于对照组(P<0.01);③高脂静养组Mankin和OARSI评分高于对照组(P<0.01)和高脂运动组(P<0.05);④对照组Ⅱ型胶原mRNA表达高于高脂运动组(P<0.05)和高脂静养组(P<0.01),高脂运动组Ⅱ型胶原mRNA表达高于高脂静养组(P<0.01);⑤高脂静养组基质金属蛋白酶13、白细胞介素1、低密度脂蛋白受体相关蛋白5、HOTAIR和赖氨酸特异性去甲基化酶1 mRNA表达高于对照组和高脂运动组(P<0.01);⑥结论:高脂饲养造成小鼠股骨髁骨质增加、膝关节软骨磨损,软骨细胞代谢紊乱,这一过程中HOTAIR的表达增加。8周中低强度跑台可减轻小鼠体质量、减缓膝关节软骨磨损、改善软骨细胞功能和下调HOTAIR表达。

关键词： HOTAITR 高脂膝骨关节炎中低强度运动 Micro CT LncRNAs

在线全文

学校读者我要写书评

暂无评论

石花菜醇提物成分分析

中国食品学报 2024年第2期24卷 269-280页

作者：田永奇罗海涛丁婷王奇升邓尚贵汪少芸福州大学生物科学与工程学院福州350108 浙江海洋大学食品与药学学院浙江舟山316022

目的:研究石花菜的成分,为其功能食品的研发提供基础数据。方法:采用薄层色谱、液-质谱联用方法分析石花菜醇提物的化学成分的丰富度。通过硅胶柱、高效液相等色谱方法对其进行分离纯化,并用气-质谱联用、核磁结合文献确证化合物结构;... 详细信息

目的:研究石花菜的成分,为其功能食品的研发提供基础数据。方法:采用薄层色谱、液-质谱联用方法分析石花菜醇提物的化学成分的丰富度。通过硅胶柱、高效液相等色谱方法对其进行分离纯化,并用气-质谱联用、核磁结合文献确证化合物结构;并对部分化合物抗氧化活性进行测定。结果:薄层色谱、液-质谱联用分析表明石花菜醇提物中含有丰富的次级代谢产物,且很可能是甾醇类成分。采用气-质谱联用方法从中鉴定到29个化合物(5个为甾醇类化合物),其中棕榈酸(46.76%)、棕榈酸乙酯(13.82%)、胆甾醇(7.20%)、胆甾-4,6-二烯(4.99%)为含量最高的4个化合物。经色谱分离、核磁鉴定出9个单体化合物,分别为胆甾醇(1)、1,2-二醇植烯(2)、帕格甾醇C(3)、6-羟基-胆甾-4-烯-3-酮(4)、胆甾-5-烯-7-酮(5)、胆甾-5-烯-3β,7α二醇(6)、胆甾-5-烯-3β,7β二醇(7)、胆甾-4-烯-6-酮(8)、3-羟乙基-2-甲基-马来酰亚胺(9)。其中,化合物7清除DPPH自由基和ABTS自由基能力最好,其IC_(50)值分别为180μg/mL和12.2μg/mL。结论:首次系统研究了石花菜醇提物的成分,化合物3、8、9为新天然产物。部分甾醇类化合物具有较好的抗氧化活性。

关键词：石花菜醇提物成分甾体抗氧化活性

Numerical simulation and experimental research on the wheel brush sampling process of an asteroid sampler

在线全文

学校读者我要写书评

暂无评论

机械工程前沿:英文版 2023年第2期18卷 205-226页

作者： haitao luo Qiming WEI Yuxin LI Junlin LI Wei ZHANG Weijia ZHOU State Key Laboratory of Robotics Shenyang Institute of AutomationChinese Academy of SciencesShenyang 110016China Institutes for Robotics and Intelligent Manufacturing Chinese Academy of SciencesShenyang 110169China School of Mechanical Engineering Shenyang Ligong UniversityShenyang 110159China

To examine the environmental characteristics of the microgravity force and the weathered layer on an asteroid surface,a symmetric wheel brush asteroid sampler is proposed for the collection of particles on the asteroid *** study the influence of the wheel brush rotation speed on the sampling efficiency and the driving torque required for the wheel brush,the contact dynamics model between particles and sampling wheel brushes is established and a simulation and experimental verification of the sampling process are *** parameter calibration of the sampled particles is studied first,and the calibrated particle parameters are used in the numerical simulation of the sampling *** sampling results and the particle stream curves are obtained for the working conditions of different rotation speeds,and the effects of different parameter settings on the sampling efficiency are *** addition,a set of rotating symmetrical sampling wheel brush devices is built for the ground test,and the dynamic torque sensor is used to test the torque change of the wheel brush during the sampling *** relationship between the speed of the wheel brush and the driving torque of the wheel brush motor is determined by comparing the simulation results with the test *** indicate that when the rotating speed of the wheel brush is faster,the sampling efficiency is higher,and the driving torque required for the sampling wheel brush is ***,a numerical simulation analysis of the sampling process of the wheel brush sampler in a microgravity environment is conducted to determine the optimal speed condition,and the brushing test of the wheel brush sampler in the microgravity environment is verified with the drop tower *** research proposes the structural optimization design and motor selection of a wheel brush asteroid sampler,which provides important reference value and engineering significance.

关键词： asteroid sampling wheel brush sampler discrete element method parameter calibration experimental research

Large-scale study of long non-coding RNA functions based on structure and expression features

在线全文

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2013年第10期56卷 953-959页

作者： ZHAO Yi WANG Jian CHEN XiaoWei luo haitao ZHAO YunJie XIAO Yi CHEN RenSheng Bioinformatics Research Group Advanced Computing Research LaboratoryInstitute of Computing TechnologyChinese Academy ofSciences Department of Physics Huazhong University of Science and Technology Laboratory of Bioinformatics and Non-coding RNA Institute of BiophysicsChinese Academy of Sciences

Mammals and other complex organisms can transcribe an abundance of long non-coding RNAs(lncRNAs)that fulfill a wide variety of regulatory roles in many biological *** roles,including as scaffolds and as guides for protein-coding genes,mainly depend on the structure and expression level of *** this review,we focus on the current methods for analyzing lncRNA structure and expression,which is basic but necessary information for in-depth,large-scale analysis of lncRNA functions.

关键词： non-coding RNA structure expression function

Genome-wide identification of cancer-related polyadenylated and non-polyadenylated RNAs in human breast and lung cell lines

在线全文

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2013年第5期56卷 503-512,1-4页

作者： ZHAO GuoGuang JIAO Fei LIAO Qi luo haitao LI Hui SUN Liang BU DeChao YU KunTao ZHAO Yi CHEN RunSheng Bioinformatic Research Group Key Laboratory of Intelligent Information ProcessingAdvanced Computing Research LaboratoryInstitute of Computing TechnologyChinese Academy of Sciences University of Chinese Academy of Sciences Department of Biochemistry and Molecular Biology Binzhou Medical College Department of Preventive Medicine School of MedicineNingbo University College of Computer Science and Technology Jilin University Bioinformatics Laboratory and National Laboratory of Biomacromolecules Institute of BiophysicsChinese Academy of SciencesBeijing 100101China

Eukaryotic mRNAs consist of two forms of transcripts:poly(A)+ and poly(A),based on the presence or absence of poly(A) tails at the 3 ***(A)+ mRNAs are mainly protein coding mRNAs,whereas the functions of poly(A) mRNA are largely *** studies have shown that a significant proportion of gene transcripts are poly(A) or bimorphic(containing both poly(A)+ and poly(A) transcripts).We compared the expression levels of poly(A) and poly(A)+ RNA mRNAs in normal and cancer cell *** also investigated the potential functions of these RNA transcripts using an integrative workflow to explore poly(A)+ and poly(A) transcriptome sequences between a normal human mammary gland cell line(HMEC) and a breast cancer cell line(MCF-7),as well as between a normal human lung cell line(NHLF) and a lung cancer cell line(A549).The data showed that normal and cancer cell lines differentially express these two forms of *** ontology(GO) annotation analyses hinted at the functions of these two groups of transcripts and grouped the differentially expressed genes according to the form of their *** data showed that cell cycle-,apoptosis-,and cell death-related functions corresponded to most of the differentially expressed genes in these two forms of transcripts,which were also associated with the ***,translational elongation and translation functions were also found for the poly(A) protein-coding genes in cancer cell *** demonstrate that poly(A) transcripts play an important role in cancer development.

关键词： polyadenylation non-polyadenylation function annotation high throughput sequencing cancer bioinformatics

同方期刊数据库

Systematic study of human long intergenic non-coding RNAs and their impact on cancer

在线全文

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2013年第4期56卷 324-334页

作者： SUN Liang luo haitao LIAO Qi BU DeChao ZHAO GuoGuang LIU ChangNing LIU YuanNing ZHAO Yi College of Computer Science and Technology Jilin University Bioinformatics Research Group Advanced Computing Research Laboratory Institute of Computing TechnologyChinese Academy of Sciences Department of Preventive Medicine School of Medicine Ningbo University

The functional impact of several long intergenic non-coding RNAs (lincRNAs) has been characterized in previous studies. However, it is difficult to identify lincRNAs on a large-scale and to ascertain their functions or predict their structures in laboratory experiments because of the diversity, lack of knowledge and specificity of expression of lincRNAs. Furthermore, although there are a few well-characterized examples of lincRNAs associated with cancers, these are just the tip of the iceberg owing to the complexity of cancer. Here, by combining RNA-Seq data from several kinds of human cell lines with chromatin-state maps and human expressed sequence tags, we successfully identified more than 3000 human lincRNAs, most of which were new ones. Subsequently, we predicted the functions of 105 lincRNAs based on a coding-non-coding gene co-expression network. Finally, we propose a genetic mediator and key regulator model to unveil the subtle relationships between lincRNAs and lung cancer. Twelve lincRNAs may be principal players in lung tumorigenesis. The present study combines large-scale identification and functional prediction of human lincRNAs, and is a pioneering work in characterizing cancer-associated lincRNAs by bioinformatics.

关键词： lincRNA identification functional annotation cancer

Single-cell Long Non-coding RNA Landscape of T Cells in Human Cancer Immunity

在线全文

学校读者我要写书评

暂无评论

Genomics, Proteomics & Bioinformatics 2021年第3期19卷 377-393页

作者： haitao luo Dechao Bu Lijuan Shao Yang Li Liang Sun Ce Wang Jing Wang Wei Yang Xiaofei Yang Jun Dong Yi Zhao Furong Li Translational Medicine Collaborative Innovation Center The Second Clinical Medical College(Shenzhen People’s Hospital)Jinan UniversityShenzhen 518020China Shenzhen Key Laboratory of Stem Cell Research and Clinical Transformation Shenzhen 518020China Integrated Chinese and Western Medicine Postdoctoral Research Station Jinan UniversityGuangzhou 510632China Bioinformatics Research Group Key Laboratory of Intelligent Information ProcessingAdvanced Computing Research CenterInstitute of Computing TechnologyChinese Academy of SciencesBeijing 100190China Department of Gastrointestinal Surgery The Second Clinical Medical College(Shenzhen People’s Hospital)Jinan UniversityShenzhen 518020China

The development of new biomarkers or therapeutic targets for cancer immunotherapies requires deep understanding of *** date,the complete landscape and systematic characterization of long noncoding RNAs(lncRNAs)in T cells in cancer immunity are ***,by systematically analyzing full-length single-cell RNA sequencing(scRNA-seq)data of more than 20,000 libraries of T cells across three cancer types,we provided the first comprehensive catalog and the functional repertoires of lncRNAs in human T ***,we developed a custom pipeline for de novo transcriptome assembly and obtained a novel lncRNA catalog containing 9433 *** increased the number of current human lncRNA catalog by 16%and nearly doubled the number of lncRNAs expressed in T *** found that a portion of expressed genes in single T cells were lncRNAs which had been overlooked by the majority of previous *** on metacell maps constructed by the MetaCell algorithm that partitions scRNA-seq datasets into disjointed and homogenous groups of cells(metacells),154 signature lncRNA genes were *** were associated with effector,exhausted,and regulatory T cell ***,84 of them were functionally annotated based on the co-expression networks,indicating that lncRNAs might broadly participate in the regulation of T cell *** findings provide a new point of view and resource for investigating the mechanisms of T cell regulation in cancer immunity as well as for novel cancer-immune biomarker development and cancer immunotherapies.

关键词： Long non-coding RNA Transcriptome assembly Metacell Immune regulation Functional annotation

Influences of chemical reactions on polysulfide reduction reaction process on promotor surface in Li-S batteries

在线全文

学校读者我要写书评

暂无评论

Nano Research 2024年第4期17卷 2712-2718页

作者： Yufeng luo Zhenhan Fang Zixin Hong Shaorong Duan haitao Liu Hengcai Wu Qunqing Li Yuegang Zhang Shoushan Fan Wenhui Duan Jiaping Wang Tsinghua-Foxconn Nanotechnology Research Center Tsinghua UniversityBeijing 100084China Department of Physics Tsinghua UniversityBeijing 100084China Laboratory of Computational Physics Institute of Applied Physics and Computational MathematicsBeijing 100088China Frontier Science Center for Quantum Information Tsinghua UniversityBeijing 100084China

Polar promotors have been proven effective in catalyzing the polysulfide(PS)reduction reaction(PSRR)process in lithium-sulfur(Li-S)***,the promotor surface tends to be poisoned due to the accumulation of insoluble discharging products of lithium disulfide(Li_(2)S_(2))and lithium sulfide(Li_(2)S)during Li-S battery ***,we investigate the detailed PSRR mechanism on the surface of manganese sulfides(MnS)as a representative promoter by performing in-situ Raman mapping *** catalytic ability of MnS enables thorough electrochemical reduction of PSs to Li_(2)S_(2) and Li_(2)S on the MnS *** generated Li_(2)S_(2) and Li_(2)S then adsorb the dissolved PSs via chemical reactions among sulfur species during the subsequent PSRR *** phenomenon mitigates promotor poisoning and continuously improves the reversible ***,the assembled Li-S cell demonstrates excellent electrochemical performance after introducing a conductive interlayer containing a thin piece of carbon nanotube film and MnS promotors.

关键词： promotors polysulfides chemical reactions catalytic ability in-situ Raman measurements

Dendritic nanomedicine enhances chemoimmunotherapy by disturbing metabolism of cancer-associated fibroblasts for deep penetration and activating function of immune cells

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Acta Pharmaceutica Sinica B 2024年第8期14卷 3680-3696页

作者： Yunkun Li Xiaoding Shen haitao Ding Yuxin Zhang Dayi Pan Liping Su Yahui Wu Zaixiang Fang Jie Zhoua Qiyong Gong Kui luo Department of Radiology Huaxi MR Research Center(HMRRC)Frontiers Science Center for Disease-Related Molecular NetworkState Key Laboratory of BiotherapyWest China HospitalSichuan UniversityChengdu 610041China Functional and Molecular Imaging Key Laboratory of Sichuan Province and Research Unit of PsychoradiologyChinese Academy of Medical SciencesChengdu 610041China Department of Radiology West China Xiamen Hospital of Sichuan UniversityXiamen 361021China

Inefficient drug penetration hurdled by the stroma in the tumor tissue leads to a diminished therapeutic effect for drugs and a reduced infiltration level of immune ***,we constructed a PEGylated dendritic epirubicin(Epi)prodrug(Epi-P4D)to regulate the metabolism of cancer-associated fibroblasts(CAFs),thus enhancing Epi penetration into both multicellular tumor spheroids(MTSs)and tumor tissues in mouse colon cancer(CT26),mouse breast cancer(4T1)and human breast cancer(MDA-MB-231)*** cytotoxicity against CT26 MTSs and remarkable antitumor efficacy of Epi-P4D were ascribed to reduced fibronectin,α-SMA,and collagen ***,thinning of the tumor tissue stroma and efficient eradication of tumor cells promoted the immunogenic cell death effect for dendritic cell(DC)maturation and subsequent immune activation,including elevating the CD4^(+)T cell population,reducing CD4^(+)and CD8^(+)T cell hyperactivation and exhaustion,and amplifying the natural killer(NK)cell proportion and effectively activating *** a result,this dendritic nanomedicine thinned the stroma of tumor tissues to enhance drug penetration and facilitate immune cell infiltration for elevated antitumor efficacy.

关键词： Dendritic prodrug Enhanced penetration Metabolic intervention Cancer-associated fibroblasts Immunogenic cell death Chemotherapy Immunotherapy Immune microenvironment reconstitution