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MiR1511 co-regulates with miR1511* to cleave the GmRPL4a gene in soybean

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Chinese Science Bulletin 2012年第28期57卷 3804-3810页

作者： luo zhongqin JIN LongGuo QIU LiJuan The National Key Faeility for Crop Gene Resources and Genetic Improvement(NFCRI) Institute of Crop ScienceChinese Academy of Agricultural SeiencesBeijing 100081China

MicroRNA1511(miR1511) is a small RNA with unknown function identified in several plants by deep *** this study,we showed that this small RNA is an authentic miRNA by analyzing the structure of the precursor stem-loop containing the newly identified miR1511 * *** confirmed this result by Northern blotting *** used 5'RACE to identify one of the target genes(GmRPL4a) cleaved by both miR1511 and miR1511 *.The site cleaved by miR1511 * was located in the first exon of GmRPL4a,and the site cleaved by miR1511 was located in the second *** expression level of miR1511/1511 * was higher in leaves than in roots and *** contrast,the lowest level of GmRPL4a expression was in the leaves and the highest in the *** results indicate that an miRNA can co-regulate with an miRNA * to cleave the same target gene in plants,and that the level of GmRPL4a mRNA is regulated by miR1511/1511 *.

关键词：靶基因切割 miRNA Northern 大豆 blot分析小RNA RACE

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低阶煤煤层气富集区预测方法研究与应用

煤田地质与勘探 2021年第6期49卷 251-257页

作者：罗忠琴刘鹏孟凡彬中国煤炭地质总局地球物理勘探研究院河北涿州072750

目前低阶煤煤层气勘探面临资源探明率低,单井产量低的问题,中、低阶煤煤层气勘探还处于摸索阶段。为提高低阶煤煤层气富集区预测精度,探索性应用"弹性模量参数反演技术"。应用韩城、晋城、保德、吉尔嘎朗图、沁水盆地、淮南、淮北等地5... 详细信息

目前低阶煤煤层气勘探面临资源探明率低,单井产量低的问题,中、低阶煤煤层气勘探还处于摸索阶段。为提高低阶煤煤层气富集区预测精度,探索性应用"弹性模量参数反演技术"。应用韩城、晋城、保德、吉尔嘎朗图、沁水盆地、淮南、淮北等地56口井652个煤样实验室测试数据,进行高、中、低阶煤含气量与弹性参量关系对比分析,结果表明:不同煤阶的煤层含气量与弹性参量之间相关关系具有一致性,且密度对低阶煤含气量的变化较中、高阶煤更为敏感,认为低阶煤煤层气储层具有利用地球物理方法进行识别的基础。在此基础上,选取吉尔嘎朗图凹陷三维地震数据,应用叠前弹性模量参数反演方法,预测其白垩系下统赛罕塔拉组Ⅳ煤组煤层气富集区面积总计101 km^(2),为下一步开发提供了优质建产区;经验证,预测结果与含气量测试结果吻合率为83.3%。叠前弹性模量参数反演可应用于低阶煤煤层气富集区预测,研究结果对低阶煤煤层气勘探开发具有一定的指导意义。

关键词：低煤阶煤层气富集区密度弹性模量参数反演

PNN概率神经网络反演技术预测煤层顶板岩性——以寺家庄煤矿中央盘区为例

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煤田地质与勘探 2018年第A1期46卷 50-55页

作者：刘最亮罗忠琴阳泉煤业(集团)有限责任公司山西阳泉045000 中国煤炭地质总局地球物理勘探研究院河北保定072750

为研究山西阳泉寺家庄煤矿构造软煤发育规律,采用PNN概率神经网络反演技术对研究区煤层顶板岩性进行反演,从而对构造软煤进行预测。以自然伽马曲线为约束条件,通过分析目标区顶板砂岩、泥岩密度与自然伽马的响应特征,对叠后三维地震数... 详细信息

为研究山西阳泉寺家庄煤矿构造软煤发育规律,采用PNN概率神经网络反演技术对研究区煤层顶板岩性进行反演,从而对构造软煤进行预测。以自然伽马曲线为约束条件,通过分析目标区顶板砂岩、泥岩密度与自然伽马的响应特征,对叠后三维地震数据进行PNN反演。结果表明:15号煤层顶板砂岩对应低自然伽马值,且与构造软煤发育呈正相关性。通过研究煤层顶板砂岩分布规律,可间接预测构造软煤的空间发育特征。

关键词：顶板岩性预测伽马测井概率神经网络

系统性红斑狼疮患者外周血CD4^+T细胞TIGIT的表达和意义

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免疫学杂志 2017年第6期33卷 512-518页

作者：黄自坤罗忠勤李雪罗清南昌大学第一附属医院检验科 330006

目的探讨TIGIT在系统性红斑狼疮(SLE)患者外周血CD4^+T细胞上的表达及临床意义,以阐明其在SLE发生和发展中的作用。方法应用流式细胞仪检测50例SLE患者和27例健康对照者外周血CD4^+T细胞表面TIGIT表达水平,比较SLE组和健康对照组以及SL... 详细信息

目的探讨TIGIT在系统性红斑狼疮(SLE)患者外周血CD4^+T细胞上的表达及临床意义,以阐明其在SLE发生和发展中的作用。方法应用流式细胞仪检测50例SLE患者和27例健康对照者外周血CD4^+T细胞表面TIGIT表达水平,比较SLE组和健康对照组以及SLE稳定组和SLE活动组之间CD4^+T细胞表面TIGIT表达水平,并分析其与实验室检查数据及治疗的相关性。2组间比较采用t检验或者非参数检验,2个变量之间相关性采用Pearson相关分析。结果 SLE组CD4^+T细胞TIGIT表达水平显著高于健康对照组(P<0.000 1),SLE活动组CD4^+T细胞TIGIT表达水平显著高于SLE稳定组(P<0.05)。SLE患者中抗ds-DNA抗体、抗Sm抗体阳性组外周血CD4^+T细胞TIGIT表达水平均高于对应阴性组(P<0.05)。SLE患者CD4^+T细胞TIGIT表达水平与SLE疾病活动度指数(SLEDAI)、尿微量白蛋白、尿Ig G、尿转铁蛋白呈正相关(P<0.05)。结论 SLE患者外周血CD4^+T细胞TIGIT表达异常,与SLEDAI、自身抗体产生、肾损伤有明确的相关性。

关键词：系统性红斑狼疮 CD4^+T细胞 TIGIT

类风湿性关节炎外周血中性粒细胞PD-L1表达及意义

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免疫学杂志 2017年第7期33卷 586-590页

作者：黄自坤李雪罗忠勤罗清南昌大学第一附属医院检验科 330006 南昌大学医学院 330006

目的探讨程序性死亡配体1(PD-L1)在类风湿性关节炎(RA)患者外周血中性粒细胞上的表达及临床意义。方法应用流式细胞仪检测54例RA患者和52例健康对照者外周血中性粒细胞PD-L1表达水平,比较RA组和健康对照组之间以及RA活动组和RA稳定组PD-... 详细信息

目的探讨程序性死亡配体1(PD-L1)在类风湿性关节炎(RA)患者外周血中性粒细胞上的表达及临床意义。方法应用流式细胞仪检测54例RA患者和52例健康对照者外周血中性粒细胞PD-L1表达水平,比较RA组和健康对照组之间以及RA活动组和RA稳定组PD-L1^+中性粒细胞百分率差异,分析其与RA实验室检查指标的相关性。结果 RA组外周血PD-L1^+中性粒细胞百分率高于健康对照组,差异有统计学意义(P=0.000 1);RA活动组PD-L1^+中性粒细胞百分率高于RA稳定组,差异有统计学意义(P=0.045 8)。RA患者PD-L1^+中性粒细胞百分率与DAS28呈正相关(r^2=0.081,P=0.037 2)。RA患者PD-L1^+中性粒细胞百分率与炎症性指标CRP和ESR呈正相关(r^2=0.12,P=0.014 9;r^2=0.14,P=0.007 2)。RA患者RF阳性组外周血PD-L1^+中性粒细胞百分率高于对应的阴性组(P=0.006 9)。RA患者PD-L1^+中性粒细胞表达的ROS水平明显高于PD-L1^-中性粒细胞(P<0.000 1)。结论 RA患者外周血中性粒细胞表面PD-L1表达异常,与疾病的活动性以及抗体产生有关。

关键词：类风湿性关节炎中性粒细胞程序性死亡配体1

类风湿关节炎患者外周血CD4＋T和CD8＋T细胞TIGIT的表达和意义

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中华微生物学和免疫学杂志 2017年第5期37卷 325-332页

作者：黄自坤李雪邓桢罗忠勤鞠北华罗清南昌大学第一附属医院检验科 330006

目的探讨含T细胞免疫球蛋白和ITIM结构域蛋白（T cell immunoreceptor with Ig and immunoreceptor tyrosine-based inhibitory domains, TIGIT）在类风湿关节炎（RA）患者外周血CD4＋T和CD8＋T细胞上的表达及临床意义,以阐明其在RA发生和发展中的作用.方法应用流式细胞仪检测81例RA患者和33例健康对照者外周血各白细胞表面TIGIT表达水平,比较RA组和健康对照组之间各白细胞表面TIGIT表达水平,并分析CD4＋T、CD8＋T细胞TIGIT表达与实验室检查数据的相关性.两组间比较采用t检验或者非参数检验,两变量之间相关性采用Pearson或Spearman相关分析.结果（1） RA组CD3＋T细胞TIGIT表达百分率高于对照组（P〈0.01）,RA组CD3＋T细胞TIGIT表达平均荧光强度（MFI）高于对照组（P〈0.01）,而在B细胞、单核细胞和中性粒细胞上表达的TIGIT无差异.（2）RA组CD4＋T和CD8＋T细胞TIGIT表达百分率显著高于健康对照组（P〈0.01）,RA组CD4＋T和CD8＋T细胞TIGIT表达MFI显著高于健康对照组（P〈0.01）.（3） RA患者外周血CD4＋T细胞TIGIT表达百分率与红细胞沉降率（ESR）呈正相关（rs=0.355, P〈0.01）;RA患者外周血CD8＋T细胞TIGIT表达百分率与ESR呈正相关（rs=0.277, P=0.013）.（4）RA患者外周血CD4＋T细胞TIGIT表达百分率与类风湿因子（RF）呈正相关（rs=0.265, P=0.017）;RA患者外周血CD4＋T细胞TIGIT表达MFI与RF呈正相关（rs=0.226, P=0.043）;RA患者外周血CD8＋T细胞TIGIT表达百分率与RF呈正相关（rs=0.366, P〈0.01）;抗CCP抗体阳性RA患者外周血CD4＋T细胞TIGIT表达百分率与抗环瓜氨酸肽（CCP）抗体呈正相关（rs=0.324, P=0.012）.（5） RA患者外周血CD4＋T细胞TIGIT表达百分率与RA疾病活动性评分（DAS28）呈正相关（r=0.232, P=0.038）;RA患者外周血CD4＋T细胞TIGIT表达MFI与DAS28呈正相关（r=0.343, P〈0.01）.结论 RA患者外周血T细胞TIGIT表达异常,与炎症水平、自身抗体产生和疾病活动性有明确的相关性.

关键词：类风湿关节炎 CD4+T细胞 CD8+T细胞

Optimal regulation of flexible loads in rural residential buildings considering mobile batteries:A case study in Shaanxi Province

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Building Simulation 2024年第7期17卷 1065-1083页

作者： Xi luo WenceShi School of Building Services Science and Engineering Xian University of Architecture and Technology(XAUAT)Xi'an 710055China State Key Laboratory of Green Building Xi'an 710055China

While the grid-connected capacity of rural household photovoltaics is increasing rapidly,achieving dynamic supply-demand matching despite fluctuations in solar energy is *** the rapid development of rural electrification,battery-powered technologies,such as electric vehicles and electric agricultural machinery,are becoming increasingly popular in rural *** this context,utilizing idle mobile batteries to assist in energy storage for rural residential buildings offers a new way to solve the problem of dynamic supply-demand *** this study,a field survey was conducted on several typical fruit-growing villages in the Central Shaanxi Plain in Shaanxi Province of *** rural households were selected to calculate the electricity loads of the residential buildings,with due consideration to the intervention of mobile *** the premise of installing 3 kW household photovoltaic systems in rural households,an economical efficiency-oriented model was built for the optimal regulation of flexible *** results were compared in the context of two patterns of electricity consumption,i.e.,unidirectional charging of mobile batteries from buildings and bidirectional charging and discharging between mobile batteries and *** bidirectional pattern significantly increased the photovoltaic consumption of typical rural households on various typical ***,during both scenarios of not implementing time-of-use and implementing time-of-use,the typical day of the winter slack farming season exhibited the best photovoltaic consumption effect among all types of typical ***,the bidirectional pattern also result in a significant increase in the annual electricity sales revenues for typical rural households.

关键词： demand-side response flexible load mobile battery household photovoltaics

Energy consumption simulations of rural residential buildings considering differences in energy use behavior among family members

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Building Simulation 2024年第8期17卷 1335-1358页

作者： Xi luo Lina Du School of Building Services Science and Engineering Xi'an University of Architecture and Technology(XAUAT)Xi'an 710055China State Key Laboratory of Green Building Xi'an 710055China

The“average occupant”methodology is widely used in energy consumption simulations of residential buildings;however,it fails to consider the differences in energy use behavior among family *** on a field survey on the Central Shaanxi Plain,to identify the energy use behavior patterns of typical families,a stochastic energy use behavior model considering differences in energy use behavior among family members was proposed,to improve the accuracy of energy consumption simulations of residential *** results indicated that the surveyed rural families could be classified into the following four types depending on specific energy use behavior patterns:families of one elderly couple,families of one middle-aged couple,families of one elderly couple and one child,and families of one couple and one ***,on typical summer days,the results of daily building energy consumption simulation obtained by the“average occupant”methodology were 25.39%and 28%lower than the simulation results obtained by the model proposed in this study for families of one elderly couple and families of one middle-aged couple,and 13.05%and 23.05%higher for families of one elderly couple and one child,and families of one couple and one *** typical winter days,for the four types of families,the results of daily building energy consumption simulation obtained by the“average occupant”methodology were 21.69%,10.84%,1.21%,and 8.39%lower than the simulation results obtained by the model proposed in this study,respectively.

关键词： typical energy use behavior pattern differences in occupant behavior building energy consumption simulation stochastic energy use behavior simulation

Switching Behavior of Cascode GaN Under Influence of Gate Driver

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CSEE Journal of Power and Energy Systems 2024年第4期10卷 1816-1833页

作者： Bin luo Guangzhao luo Sihai Li School of Automation Northwestern Polytechnical UniversityXi’an 710072China

With high-frequency,low power dissipation and high-efficiency characteristics,Gallium nitride(GaN)power devices are of significant benefit in designing high-speed motor drives,as they improve performance and reduce ***,due to the cascode structure,coupling with the parasitics in gate driver and power circuits,power converters based on cascode GaN are prone to overshoot and oscillate on switching waveforms,which may lead to serious EMC problems,or even device *** complicated structure of cascode GaN device makes the gate driver design comparatively *** analytical model of the switching process considering gate driver parameters is proposed in this *** influence of gate driver parameters on switching behavior is investigated from the perspective of switching speed,waveform overshoot,and power ***-offs among overshoot,switching speed,and power loss are discussed;guidelines to design gate driver parameters are given.

关键词： Gallium nitride(GaN) gate driver oscillation and overshoot switching characteristics