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LNP-CpG ODN-adjuvanted varicella-zoster virus glycoprotein E induced comparable levels of immunity with Shingrix^(TM) in VZV-primed mice

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Virologica Sinica 2022年第5期37卷 731-739页

作者： ning luan Han Cao Yunfei Wang Kangyang Lin Cunbao Liu Institute of Medical Biology Chinese Academy of Medical Sciences and Peking Union Medical CollegeKunming 650118China

Latent varicella-zoster virus(VZV)may be reactivated to cause herpes zoster,which affects one in three people during their *** currently available subunit vaccine Shingrix^(TM) is superior to the attenuated vaccine Zostavax®in terms of both safety and efficacy,but the supply of its key adjuvant component QS21 is *** ionizable lipid nanoparticles(LNPs)that were recently approved by the FDA for COVID-19 mRNA vaccines as carriers,and oligodeoxynucleotides containing CpG motifs(CpG ODNs)approved by the FDA for a subunit hepatitis B vaccine as immunostimulators,we developed a LNP vaccine encapsulating VZV-glycoprotein E(gE)and CpG ODN,and compared its immunogenicity with Shingrix^(TM) in C57BL/6J *** results showed that the LNP vaccine induced comparable levels of gE-specific IgG antibodies to Shingrix^(TM) as determined by enzymelinked immunosorbent assay(ELISA).Most importantly,the LNP vaccine induced comparable levels of cellmediated immunity(CMI)that plays decisive roles in the efficacy of zoster vaccines to Shingrix^(TM) in a VZVprimed mouse model that was adopted for preclinical studies of Shingrix^(TM) .Number of IL-2 and IFN-γsecreting splenocytes and proportion of T helper 1(Th1)cytokine-expressing CD4^(+)T cells in LNP-CpG-adjuvanted VZV-gE vaccinated mice were similar to that of Shingrix^(TM) boosted *** of the components in this LNP vaccine can be artificially and economically synthesized in large quantities,indicating the potential of LNP-CpGadjuvanted VZV-gE as a more cost-effective zoster vaccine.

关键词： Varicella zoster virus(VZV) Subunit vaccine Adjuvant Lipid nanoparticle(LNP) CpG oligodeoxynucleotide(CpG ODN) AS01B Humoral immunity Cell-mediated immunity(CMI)

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Capacity of Tea Scavenging Singlet Oxygen Studied by Means of 1,3-Diphenylisobenzofuran as Fluorescence Probe

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Chemical Research in Chinese Universities 2009年第6期25卷 966-970页

作者： LU Qing-luan YUE ning-ning ZHANG Miao GONG Bin WANG Huai-you College of Chemistry Chemical Engineering and Materials Science Shandong Normal University Jinan 250014 P R. China

In recent years, the effects of reactive oxygen species（ROS） generated in the course of biological metabolism, such as superoxide（O2 ）, hydrogen peroxide（H202）, hydroxyl radical（HO） and singlet oxygen（102） on the human health have received more attention due to their vital roles in physiological functions. Normally, antioxidant molecules, superoxide dismutase and catalase in biological organism can scavenge excessive free radicals by a series of chemical reactions to keep the cells in a state of redox homeostasis. Once the homeostasis has been broken,

关键词： Singlet oxygen 1,3-Diphenylisobenzofuran Methylene blue Tea leaf Fluorescence quenching

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Preliminary investigation into feasibility of dissolved methane measurement using cavity ringdown spectroscopy technique

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Frontiers of physics 2016年第6期11卷 159-166页

作者： Zhen-Nan Wang Wang-Quan Ye Xiao-ning luan Fu-Jun Qi Kai Cheng Ronger Zheng Optics and Optoelectronies Laboratory Ocean University of China Qingdao 266100 China

For the exploration of gas hydrate resources by measuring the dissolved methane concentration in seawater, a continuous-wave cavity ringdown spectroscopy (CW-CRDS) experimental setup was constructed for trace methane detection. A current-modulation method, rather than a cavity-modulation method using an optical switch and a piezoelectric transducer, was employed to realize the cavity excitation and shutoff. Such a current-modulation method enabled the improvement of the experimental setup construction and stability, and the system size and stability are critical for a sensor to be deployed underwater. Ringdown data acquisition and processing were performed, followed by an evaluation of the experimental setup stability and sensitivity. The obtained results demonstrate that great errors are introduced when a large fitting window is selected if the analog-to-digital converter has an insufficient resolution. The ringdown spectrum of methane corresponding to the 2 nu(3) band R(4) branch was captured, and the methane concentration in lab air was determined to be 2.06 ppm. Further experiments for evaluating the quantitative ability of this CW-CRDS experimental setup are underway from which a high-sensitivity methane sensor that can be combined with a degassing system is expected.

关键词： gas hydrate methane continuous-wave cavity ringdown spectroscopy current-modulation method

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In vivo antimalarial activity of synthetic hepcidin against Plasmodium berghei in mice

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Chinese Journal of Natural Medicines 2017年第3期15卷 161-167页

作者： FANG Ya-Qun SHEN Chuan-Bin luan ning YAO Hui-Min LONG Chen-Bo LAI Ren YAN Xiu-Wen College of Life Sciences Nanjing Agricultural UniversityNanjing 210095China Key Laboratory of Animal Models and Human Disease Mechanisms Kunming Institute of ZoologyChinese Academy of SciencesKunming 650223China University of Chinese Academy of Sciences Beijing 100009China

The present study was designed to investigate the antimalarial activity of synthetic hepcidin and its effect on cytokine secretion in mice infected with Plasmodium *** mice were infected with *** intravenously and treated with hepcidin according to 4-day suppression test and Rane's *** serum levels of interleukins(IL-1β,IL-2,IL-6,IL-10,IL-12p70,and IL-17A),tumor necrosis factor-α(TNF-α),and interferon-γ(IFN-γ) in the experimental mice were determined using a cytometric bead array(CBA)*** survival rate of the infected mice was also ***,the serum iron,alanine transaminase(ALT),aspartate transaminase(AST),and total bilirubin(BIL) were detected to evaluate liver *** exerted direct anti-malarial function in vivo and increased survival rate in a dose-dependent *** addition,the secretion of T helper cell type 1(Th1),Th2,and Th17 cytokines,TNF-α,and IFN-γ were inhibited by *** conclusion,our results demonstrated that synthetic hepcidin exerts in vivo antimalarial activity and possesses anti-inflammatory function,which provides a basis for future design of new derivatives with ideal anti-malarial activity.

关键词： Hepcidin Malaria Cytokine Inflammation Iron

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Antimicrobial peptide LL-37 forms complex with bacterial DNA to facilitate blood translocation of bacterial DNA and aggravate ulcerative colitis

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Science Bulletin 2018年第20期63卷 1364-1375页

作者： Zilei Duan Yaqun Fang Yang Sun ning luan Xue Chen Mengrou Chen Yajun Han Yizhu Yin James Mwangi Junkun Niu Kunhua Wang Yinglei Miao Zhiye Zhang Ren Lai Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences/Key Laboratory of Bioactive Peptides of Yunnan Province Kunming Institute .of ZoologyKunming 650223China Life Sciences College of Nanjing Agricultural University Nanjing 210095China Department of Gastroenterology The First Affiliated Hospital of Kunming Medical UniversityKunming 650032China Department of General Surgery The First Affiliated Hospital of Kunming Medical UniversityKunming 650032China Kunming College of Life Science University of Chinese Academy of SciencesKunming 650204China Sino-African Joint Research Center Kunming Institute of ZoologyChinese Academy of SciencesKunming 650223China

Bacterial DNA(bacDNA) is frequently found in serum of patient with ulcerative colitis(UC) and Crohn's disease, even blood bacterial culture is negative. How bacDNA evades immune elimination and is translocated into blood remain unclear. Here, we showed that bacDNA avoids elimination and disables bacteriakilling function of antimicrobial peptide LL-37(Cramp in mice) by forming complex with LL-37, which is inducible after culture with bacteria or bacterial products. Elevated LL-37-bacDNA complex was found in plasma and lesions of patients with UC. LL-37-bacDNA promoted inflammation by inducing Th1, Th2 and Th17 differentiation and activating toll-like receptor-9(TLR9). The complex also increased paracellular permeability, which possibly combines its inflammatory effects to promote local damage and bacDNA translocation into blood. Cramp-bacDNA aggravated mouse colitis severity while interference with the complex ameliorated the disease. The study identifies that inflammatogenic bacDNA utilizes LL-37 as a vehicle for blood translocation and to evade immune elimination. Additionally, bacteria may make a milieu by releasing bacDNA to utilize and resist host antimicrobial peptides as a ‘trojan horse'.

关键词： Ulcerative colitis Bacterial DNA LL-37 Immune elimination Chronic inflammation

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A Novel Trypsin Inhibitor-Like Cysteine-Rich Peptide from the Frog Lepidobatrachus laevis Containing ProteinaseInhibiting Activity

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Natural Products and Bioprospecting 2015年第4期5卷 209-214页

作者： Yu-Wei Wang Ji-Min Tan Can-Wei Du ning luan Xiu-Wen Yan Ren Lai Qiu-Min Lu Life Sciences College of Nanjing Agricultural University Nanjing 210095JiangsuChina Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences&Yunnan Province Kunming Institute of ZoologyKunming 650223YunnanChina

Various bio-active substances in amphibian skins play important roles in survival of the *** protease inhibitor peptides have been identified from amphibian skins,which are supposed to negatively modulate the activity of proteases to avoid premature degradation or release of skin peptides,or to inhibit extracellular proteases produced by invading ***,there is no information on the proteinase inhibitors from the frog Lepidobatrachus laevis which is unique in South *** this work,a cDNA encoding a novel trypsin inhibitor-like(TIL)cysteine-rich peptide was identified from the skin cDNA library of *** 240-bp coding region encodes an 80-amino acid residue precursor protein containing 10 *** sequence comparison and signal peptide prediction,the precursor was predicted to release a 55-amino acid mature peptide with amino acid sequence,*** mature peptide was named ***-TIL shares significant domain similarity with the peptides from the TIL supper *** and trypsin-inhibitory abilities of recombinant LL-TIL were *** LL-TIL showed no antimicrobial activity,while it had trypsin-inhibiting activity with aKi of 16.5178 *** results suggested there was TIL peptide with proteinase-inhibiting activity in the skin of frog *** the best of our knowledge,this is the first report of TIL peptide from frog skin.

关键词： Trypsin inhibitor Cysteine-rich peptide Amphibian Skin

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税收优惠、营商环境与企业创新绩效

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税收经济研究 2024年第3期29卷 68-76页

作者：匡萍栾宁聊城大学商学院(质量学院)

文章基于2012—2022年A股上市公司数据,实证检验税收优惠对企业创新绩效的影响效应、路径以及营商环境在创新转化过程中的调节作用。结果表明,税收优惠能够显著提高企业创新绩效,研发投入在其中起到部分中介作用。此外,总体营商环境的... 详细信息

文章基于2012—2022年A股上市公司数据,实证检验税收优惠对企业创新绩效的影响效应、路径以及营商环境在创新转化过程中的调节作用。结果表明,税收优惠能够显著提高企业创新绩效,研发投入在其中起到部分中介作用。此外,总体营商环境的优化能够正向调节研发投入和创新绩效之间的关系。异质性分析表明,对于国有企业、融资约束程度低企业、成熟期企业,税收优惠的创新激励效应更加明显。为加快形成新质生产力、激发企业创新活力,应继续完善税收优惠政策,同时优化营商环境,保障政策实施成效。

关键词：税收优惠创新绩效营商环境研发投入

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非完整系统在Poisson白噪声下的广义F-P方程

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辽宁师范大学学报（自然科学版） 2006年第2期29卷 172-175页

作者：栾江宁尚玫内蒙古医学院公共教育学院内蒙古呼和浩特010059 北京理工大学理学院北京100081

讨论非完整系统响应的概率密度函数,首先给出非完整系统在Poisson白噪声下的概率密度函数所满足的广义Fokker-Planck方程,然后引入扰动法,以激励为高斯白噪声下的F-P-K方程的精确的平稳解为基本解,利用扰动方法来获得激励为Poisson白噪... 详细信息

讨论非完整系统响应的概率密度函数,首先给出非完整系统在Poisson白噪声下的概率密度函数所满足的广义Fokker-Planck方程,然后引入扰动法,以激励为高斯白噪声下的F-P-K方程的精确的平稳解为基本解,利用扰动方法来获得激励为Poisson白噪声时的各次修正项.

关键词：非完整系统概率密度函数 Gauss白噪声 Poisson白噪声

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浅谈机动车维修企业技术负责人应该具备的素质

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交通企业管理 2011年第2期26卷 20-21页

作者：栾宁吉林省交通运输厅物资供应站

在机动车维修企业中，技术负责人扮演着重要的角色，其在维修工艺的组织、技术标准规范的实施和质量的控制等方面发挥着至关重要的作用。GB／T21338-2008规定，技术负责人应具备两个基本条件：一是具有机动车维修或相关专业大专以上学... 详细信息

在机动车维修企业中，技术负责人扮演着重要的角色，其在维修工艺的组织、技术标准规范的实施和质量的控制等方面发挥着至关重要的作用。GB／T21338-2008规定，技术负责人应具备两个基本条件：一是具有机动车维修或相关专业大专以上学历，或具有机动车维修或相关专业的中级以上专业技术职称；二是具有在机动车维修企业5年以上的工作实践。具体归纳起来，技术负责人应具备9个方面素质。

关键词：技术负责人机动车维修维修企业素质专业技术职称标准规范 GB

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鞍山地区造林密度对林木生长的影响研究

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林业科技通讯 2019年第12期 44-45页

作者：栾宁岫岩满族自治县不动产登记中心

本文以抚顺地区30 a油松(Pinus tabuliformis)人工林为研究对象,测定了不同栽植密度对林木胸径、冠幅、树高、枝下高以及生物量等相关林木生长参数的影响,研究结果表明:胸径和冠幅随着林分密度的增加而逐渐减少;树高和高径比随着林分密... 详细信息

本文以抚顺地区30 a油松(Pinus tabuliformis)人工林为研究对象,测定了不同栽植密度对林木胸径、冠幅、树高、枝下高以及生物量等相关林木生长参数的影响,研究结果表明:胸径和冠幅随着林分密度的增加而逐渐减少;树高和高径比随着林分密度增大而呈现先降低后升高的趋势;枝下高随林分密度的增大而递增;枝下高与树高比则随密度增加而先增加后趋稳定;单株总生物量随林分密度的增大而呈现"先减小后增加再减少"的趋势。

关键词：油松 Pinus tabuliformis 密度林木生长生物量

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