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Genetic Diversity of Maize Populations Developed by Two Kinds of Recurrent Selection Methods Investigated with SSR Markers

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Agricultural Sciences in China 2008年第9期7卷 1037-1045页

作者： LI lu-jiang yang Ke-cheng PAN Guang-tang RONG Ting-zhao Maize Research Institute Sichuan Agricultural University~Key Laboratory of Crop Genetic Resources and Improvement Ministry of Education Ya＇ an 625014 P.R.China

Two cycles of biparental mass selection （MS） and one cycle of half-sib-S3 family combining selection （HS-S3） for yield were carried out in 2 synthetic maize populations P4C0 and P5C0 synchronously. The genetic diversity of 8 maize populations, including both the basic populations and their developed populations, were evaluated by 30 SSR primers. On the 30 SSR loci, a total of 184 alleles had been detected in these populations. At each locus, the number of alleles varied from 2 to 14, with an average of 6.13. The number and ratio of polymorphic loci in both the basic populations were higher than those of their developed populations, respectively. There was nearly no difference after MS but decreased after HS-S3 in both the basic populations in the mean gene heterozygosity. The mean genetic distance changed slightly after MS but decreased in a bigger degree after HS-S3 in both the basic populations. Analyses on the distribution of genetic distances showed that the ranges of the genetic distance were wider after MS and most of the genetic distances in populations developed by HS-S3 were smaller than those in both the basic populations. The number of genotypes increased after MS but decreased after HS-S3 in both the basic populations. The genetic diversity of intra-population was much more than genetic diversity of inter-population in both the basic populations. All these indexes demonstrated that the genetic diversity of populations after MS was similar to their basic populations, and the genetic diversity was maintained during MS, whereas the genetic diversity of populations decreased after HS-S3. This result indicated that heterogeneity between some of the individuals in the developed populations increased after MS, whereas the populations become more homozygotic after HS-S3.

关键词： maize recurrent selection genetic diversity SSR markers biparental mass selection (MS) half-sib-S3 familycombining selection (HS-S3)

Development of a new differential extensometer

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Geodesy and Geodynamics 2011年第1期2卷 77-80页

作者： lu Chongwu yang jiang Institute of Seismology CEA Wuhan 430071 China

This paper describes a new differential extensometer, which has a baseline rod in suspension with both ends free, and some preliminary test results. Compared with a traditional differential extensometer, which has one end of the baseline rod fixed to the ground, this instrument is less affected by commonly encountered interferences ,including environment vibration, momentary power failure, and power noise.

关键词： extensometer strain differential structure test

In vivo administration of Fms-like tyrosine kinase-3 ligand effectively stimulates lung dendritic cell expansion in mice

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Chinese Medical Journal 2012年第14期125卷 2562-2567页

作者： WANG Hong-wei lu jiang-yang WANG Lin TIAN Guang Department of Pathology First Affiliated Hospital of GeneralHospital of People's Liberation Army Beijing 100048 China Department of Stomatology General Hospital of People'sLiberation Army Beijing 100853 China

Background Dendritic ceils （DCs） are the most important professional antigen presenting cells that play a key role in initiating adaptive immune responses. The depletion and dysfunction of DCs contribute to the development of immunodeficiency or immunoparalysis in some lung diseases. In the present study, we investigated the effects of Fms-like tyrosine kinase-3 ligand （FIt3L） administration in vivo on lung DCs expansion to provide an experimental basis of FIt3L used as a potential therapeutic agent for the related lung disorders. Methods Balb/c mice were randomly divided into FIt3L group （n=10） and control group （n=10）. Each mouse in the FIt3L group received subcutaneous administration of FIt3L at a dose of 10 pg once daily for nine consecutive days. lung histology was observed, and CD11c and CD205 were immunologically labeled in lung tissue sections. Low-density lung cells were separated by density gradient centrifugation, and then subsets and MHC-II/I-Ad expression of DCs were analyzed by flow cytometry. Results In the FIt3L group the number and density of DC-like cells were markedly increased compared with the control group, mainly distributed in the alveolar septa. Immunological labeling in situ found that there were significantly higher numbers of CD11c＋ and CD205＋ DCs in lung mesenchymal tissue （P 〈0.05）, where they formed a denser reticular formation. Flow cytometry analysis demonstrated that the proportions of myeloid CD11c＋CD11b＋ DCs and plasmacytoid CD11c＋CD45R/B220＋ DCs in the low-density lung cells in the FIt3L group were significantly higher compared with the control group; showing 3.17- and 3.3-fold increase respectively （P 〈0.05）. The proportion of CD11c＋ DCs expressing MHC-II/I-Ad＋ was significantly increased, with a 2.7-fold increase as compared with the control group （P 〈0.05）. Conclusions FIt3L administration in vivo induces lung DCs expansion, favoring myeloid and plasmacytoid DC subsets, which are phenotypically more mature. FIt3L may be useful

关键词： Fins-like tyrosine kinase-3 ligand lung, dendritic cell, MHC-II CD205

The impact of nanoporous SiN_x interlayer growth position on high-quality GaN epitaxial films

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Chinese Science Bulletin 2011年第25期56卷 2739-2743页

作者： MA ZiGuang XING ZhiGang WANG XiaoLi CHEN Yao XU PeiQiang CUI YanXiang WANG lu jiang yang JIA HaiQiang CHEN Hong Beijing National Laboratory of Condensed Matter Institute of Physics Chinese Academy of Sciences Beijing 100190 China

The impact of nanoporous SiN x interlayer growth position on high-quality GaN epitaxial film was elucidated from the behavior of dislocations. The best quality GaN film was achieved when a nanoporous SiN x interlayer was grown on a rough layer, with the high-resolution X-ray diffraction rocking curve full width at half maximum for ( 1102 ) reflection decreasing to 223 arcs, and the total dislocation density reduced to less than 1.0×10 8 cm 2 . GaN films were grown on sapphire substrates by metal organic chemical vapor deposition. The quality of these films was investigated with high-resolution X-ray diffraction, atomic force microscopy, and cross-sectional transmission electron microscopy. A preference for the formation of half-loops to reduce threading dislocations was observed when an SiN x interlayer was grown on a rough layer. A growth mechanism is proposed to explain this preference.

关键词：氮化硅外延片多孔层间氮化镓 GaN薄膜化学气相沉积法透射电子显微镜

Preparation and properties of AZO thin films on different substrates

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Progress in Natural Science:Materials International 2010年第1期20卷 44-48页

作者： Hong-lie SHEN,Hui ZHANG,Lin-feng lu,Feng jiang,Chao yang College of Materials Science and Engineering, Nanjing University of Aeronautics and Astronautics,Nanjing 210016,China College of Materials Science and Engineering Nanjing University of Aeronautics and Astronautics Nanjing 210016 China

Al doped ZnO(AZO)films were deposited on glass,polyethylene naphthalate(PEN)and polyethylene terephthalate(PET) at room temperature by radio frequency magnetron *** structural,surface morphology,electrical and optical properties of the AZO films were characterized by X-ray diffractometry(XRD),atomic force microscopy(AFM),Hall effect measurement and ultraviolet-visible spectrometer,*** films exhibit highly c-axis preferred orientation,and AZO film on glass exhibits compressive stress while that on plastic substrates presents tensile *** electrical property of the AZO film on glass is the best among the films on three kinds of *** value of the figure of merit for the AZO film on PEN is much better than that of the AZO film on PET and is close to that of AZO on glass in the wavelength range of 500-900 nm.

关键词： AZO magnetron sputtering electrical and optical property plastic substrate glass substrate

Ground water hydrochemical characteristics: seawater intruded area in eastern and southern coast of Laizhou Bay

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Journal of Geographical Sciences 2001年第3期11卷 350-355页

作者： ZHANG Zu-lu, jiang lu-guang, yang Li-yuan, QI Yong-hua (Department of Geography, Shandong Normal University, Jinan 250014, China) Shandong Normal University Department of Geography Jinan China (GRID:grid.410585.d)

Eastern and southern coastal zones of Laizhou Bay are the most representative seawater intruded areas in the world, with two intrusion sources of contemporary seawater and paleobrine. In order to reveal the complicated hydrochemical changing process and the mechanism of fresh groundwater being polluted by saltwater, we conducted long-term observation and hydrochemical analysis at four observing sections of typical salt-fresh water transitional zone. The study indicates that seawater and brine intrusion processes have different hydrochemical features, and that ion exchange and adsorption actions between water and aquifer produce great influence on the intrusion.

关键词： seawater intrusion eastern and southern coastal areas of Laizhou Bay hydrochemical characteristics of groundwater

Massive hemoptysis and hemothorax： a rare but fatal complication of intralobar sequestration

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Chinese Medical Journal 2012年第14期125卷 2638-2640页

作者： WANG Hong-wei lu jiang-yang SUN Jun-zhong, XIAO Yan WEN Bo Department of Patlaology First Affiliated Hospital of General Hospital of People's Liberation Army Beijing 100048 China Department of OncologyFirst Affiliated Hospital of General Hospital of People's Liberation Army Beijing 100048 China Respiratory Center First Affiliated Hospital of General Hospitalof People's Liberation Army Beijing 100048 China Department ofRadiology First Affiliated Hospital of General Hospitalof People's Liberation Army Beijing 100048 China

Intralobar sequestration （ILS） is an uncommon abnormality that accounts for 75% of all pulmonary sequestrations. Over the years there have been several reports of various presenting signs of which hemoptysis was commonly described, however, massive hemoptysis and hemothorax is extremely rare in literature. We present a case of a 45-year-old man who died of fatal complication from an ILS. This case report shows an uncommon presentation of ILS with massive hemoptysis and hemothorax resulting in a dramatic course of disease and a fatal outcome, and for this reason in the absence of trauma or other causes for massive hemoptysis, hemothorax, or lung hematoma, this possibility should be kept in mind so as to avoid misdiagnosis, and resection of the sequestered tissue should be considered in all patients.

关键词： hemoptysis hemothorax bronchopulmonary sequestration, autopsy

Synthesis of Conformationally LockedIsonucleosides: 2'-Deoxy-2'-nucleobase-5'-deoxy-1', 4' : 3' , 6'-dianhydro-D-mannitol

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Chemical Research in Chinese Universities 2001年第2期17卷 178-185页

作者： TIAN Xiao-bing, ZHANG Ling, MIN Ji-mei and ZHANG Li-he (School of Pharmaceutical Sciences, Beijing Medical University, Beijing 100083, P. R. China) lu yang, jiang Reng-wang and ZHENG Qi-tai (Institute of Materia Medica, Peking Union Medical College &. Ch Beijing Med Univ Sch Pharmaceut Sci Beijing 100083 Peoples R China Peking Union Med Coll Inst Mat Med Beijing 100050 Peoples R China Chinese Acad Med Sci Beijing 100050 Peoples R China

Novel bicyclic isonucleosides, 2′-deoxy-2′ -nucleobase-5′-deoxy-1′ , 4′ : 3′ , 6′ -dianhydro- D-mannitol 10a-10c, were synthesized from D-glucose. The computer-assisted molecular simu- lation indicated that the... 详细信息

关键词： Bicyclic isonucleoside Locked conformation Synthesis

单/双侧选择性脑灌注在主动脉弓替换术中的脑保护效果观察

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解放军医学杂志 2015年第6期40卷 484-487页

作者：盛炜陆江阳军事医学科学院放射与辐射医学研究北京100039 解放军总医院第一附属医院病理科北京100037

目的比较单侧顺行选择性脑灌注(USCP)和双侧顺行选择性脑灌注(BSCP)在DeBakeyⅠ型主动脉夹层行主动脉弓替换术中的脑保护效果。方法回顾性分析2013年12月—2014年12月在解放军总医院心血管外科行主动脉弓替换术的24例DeBakeyⅠ型主动脉... 详细信息

目的比较单侧顺行选择性脑灌注(USCP)和双侧顺行选择性脑灌注(BSCP)在DeBakeyⅠ型主动脉夹层行主动脉弓替换术中的脑保护效果。方法回顾性分析2013年12月—2014年12月在解放军总医院心血管外科行主动脉弓替换术的24例DeBakeyⅠ型主动脉夹层患者的病例资料,根据脑灌注方式不同,分为USCP组(n=16)和BSCP组(n=8),术中深低温停循环时分别采用单泵单管(USCP组)或单泵双管(BSCP组)灌注进行脑保护。两组均于术前和术后行头颅CT检查。术中常规连续监测脑氧饱和度,观察并记录两组患者术后发生短暂神经系统功能不全(TND)的情况。结果两组均无术后早期死亡,患者均痊愈出院。术后USCP组有3例出现TND,BSCP组1例出现TND;术前及术后两组CT检查均无明显异常。体外循环中各阶段两组脑氧饱和度差异均无统计学意义(P>0.05)。结论在DeBakeyⅠ型主动脉夹层全弓替换术中使用单泵单管行单侧选择性脑灌注操作简便、切实可行,能取得良好的脑保护效果,临床预后满意。

关键词：体外循环动脉瘤夹层停循环深低温诱导脑灌注