检索结果-南通市图书馆

Downregulation of wild-type p53 protein by HER-2/neu mediated PI3K pathway activation in human breast cancer cells: its effect on cell proliferation and implication for therapy

在线全文

学校读者我要写书评

暂无评论

Cell Research 2004年第6期14卷 497-506页

作者： Li ZHENG, Jia Qiang REN, Hua LI, Zhao lu KONG, hong guang ZHU Department of Pathology, Shanghai Medical College, Fudan University, Shanghai, 200032, China. Institute of Irradiation Medicine, Fudan University, Shanghai, 200032, China Department of Pathology Shanghai Medical College Fudan University Shanghai China Institute of Irradiation Medicine Fudan University Shanghai China

Overexpression and activation of HER-2/neu (also known as c-erbB-2), a proto-oncogene, was found in about 30% of human breast cancers, promoting cancer growth and making cancer cells resistant to chemo- and radio-therapy. Wild-type p53 is crucial in regulating cell growth and apoptosis and is found to be mutated or deleted in 60-70% of human cancers. And some cancers with a wild-type p53 do not have normal p53 function, suggesting that it is implicated in a complex process regulated by many factors. In the present study, we showed that the overexpression of HER-2/neu could decrease the amount of wild-type p53 protein via activating PI3K pathway, as well as inducing MDM2 nuclear translocation in MCF7 human breast cancer cells. Blockage of PI3K pathway with its specific inhibitor LY294002 caused Gl-S phase arrest, decreased cell growth rate and increased chemo- and radio-therapeutic sensitivity in MCF7 cells expressing wild-type p53. However, it did not increase the sensitivity to adriamycin in MDA-MB-453

关键词： HER-2/neu PI3K breast cancer p53 MDM2

Adipose-derived Stem cells

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Adipose-derived Stem cells

Advanced Glycation End Products and Skin:A Review

中华医学会第十八次全国皮肤性病学术年会

作者： Bin lu,hong-guang lu The Affiliated Hospital of Guiyang Medical College

The adipose-derived stem cell(ASCs) is one of stem cells that isolated from *** some factors such as age,body mass index(BMI) and site selection have no impact on the numbers and viability ASCs,some people found ASCs in superficial abdominal fat to be the most resistant to apoptosis,and the abdomen is the optimum harvest *** for harvest of fat grafts affect not only viability of

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Advanced Glycation End Products and Skin:A Review

Edaravone attenuates ischemia-reperfusion injury by inhibiting oxidative stress in a canine lung transplantation model

中华医学会第十八次全国皮肤性病学术年会

作者： Bin lu,hong-guang lu The Affiliated Hospital of Guiyang Medical College

Advanced glycation end products(AGEs) are products of the final non-enzymatic glycation reaction,they produced by some *** are well known as they are associated with some diseases,such as diabetics and diabetics-related ***,AGEs are associated with *** accumulation has been described in dermal proteins such as fibronectin,collagen,and elastin in the skin,and AGEs

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Chinese Medical Journal 2008年第16期121卷 1583-1587页

作者： XU Jin-zhi SHEN Bao-zhong LI Ye ZHANG Tong XU Wan-hai LIU Xiao-wei lu hong-guang Department of Thoracic Surgery Fourth Affiliated Hospital of Harbin Medical University Harbin Heilongjiang 150001 China Department of Medical Imaging Fourth Affiliated Hospital of Harbin Medical University Harbin Heilongjiang 150001 China

Background Previous reports have confirmed that edaravone has protective effects against ischemia-reperfusion （IR） injury of many organs. In this study, we investigated the effect of edaravone on preventing IR injury of the lung in a canine lung transplantation model. Methods Twelve weight-matched pairs of random-bred dogs were randomized into two groups. Within each pair, one dog served as donor and the other as recipient. In the study group, prostaglandin EI（PGE1）（8 μg/kg） was injected into the donor pulmonary artery （PA） before occlusion and the donor lungs were flushed with 1.0 L of LPD solution containing edaravone （10 mg/kg） and stored in the same LPD solution at a temperature of 1℃for 8 hours. The left single lung transplantation was then performed and recipients received intravenous injection with edaravone （10 mg/kg） at the onset of reperfusion. In the control group, edaravone was substituted by the same volume of sterile saline solution. Another six dogs were obtained as normal control group in which left lungs were dissected after thoracotomy without an IR injury. One hour after reperfusion, or after dissection of the left lung, the right lung was excluded from perfusion and ventilation after which, cardiopulmonary parameters were measured. Wet/dry ratios, malondialdehyde （MDA） and myeloperoxidase （MPO） levels were assessed and histological analysis of lung tissue performed at the same time. Results All animals survived until the end of the experiment. The study group showed significantly decreased wet/dry ratios （treated：（74.1±4.2）% vs control：（86.8±5.2）%, P 〈0.01）, MDA levels （treated： 0.50±0.08 vs control： 0.88±0.15, P 〈0.01） and MPO activity （treated： 0.23±0.05 vs control： 0.43±0.07, P 〈0.01） compared to the control group two hours after occlusion of the right side. In the control group, pulmonary vascular resistance （PVR） was increased markedly and arterial oxygen partial pressure deteriorated significantly after exclusion of the right side compare

关键词： edaravone ischemia-reperfusion injury lung transplantation oxidative stress reactive oxygen species

在线全文

学校读者我要写书评

暂无评论

基于广州环城游憩带的清远旅游发展探究

特区经济 2011年第4期 172-173页

作者：陆红光清远职业技术学院广东清远511500

随着城镇居民闲暇时间的增多,城市周边区县成为市民假日旅游的重要目的地;环城游憩带作为一种新兴的旅游地域,以其独有的地域特征和良好的生态环境而成为城市居民休闲度假的首选之地。文章重点阐述广州环城游憩带对清远旅游发展的影响,... 详细信息

随着城镇居民闲暇时间的增多,城市周边区县成为市民假日旅游的重要目的地;环城游憩带作为一种新兴的旅游地域,以其独有的地域特征和良好的生态环境而成为城市居民休闲度假的首选之地。文章重点阐述广州环城游憩带对清远旅游发展的影响,并以此为基础,对清远旅游发展提出探讨,以探索清远旅游的可持续发展之路。

关键词：广州环城游憩带清远旅游

用遗传算法解决基于分条技术的磁盘负载均衡问题

在线全文

学校读者我要写书评

暂无评论

计算机学报 2006年第11期29卷 1995-2002页

作者：倪云竹吕光宏黄彦辉四川大学计算机学院成都610064

如何提高存储子系统的I/O性能一直以来都是计算机领域的一个研究热点,而目前提高存储子系统的I/O性能的一个最大障碍就是负载不均衡.文章通过研究磁盘负载均衡问题,提出了一种采用遗传算法来实现基于分条技术的磁盘动态负载均衡算法.该... 详细信息

如何提高存储子系统的I/O性能一直以来都是计算机领域的一个研究热点,而目前提高存储子系统的I/O性能的一个最大障碍就是负载不均衡.文章通过研究磁盘负载均衡问题,提出了一种采用遗传算法来实现基于分条技术的磁盘动态负载均衡算法.该方法包括基于分条技术的文件划分算法和为实现负载均衡的文件分配算法.根据遗传算法对种群进行编码、选择、杂交操作和变异操作,从而产生出满足负载均衡条件的映射方案.最后通过实验仿真证明该算法正确有效.

关键词：存储磁盘阵列磁盘映射负载均衡分条技术遗传算法

在线全文

学校读者我要写书评

暂无评论

面部糖皮质激素依赖性皮炎

临床皮肤科杂志 2006年第10期35卷 682-684页

作者：陆洪光贵阳医学院附属医院皮肤科贵州贵阳550004

面部糖皮质激素(以下简称激素)依赖性皮炎已成为皮肤科的常见病,长期滥用或误用激素类制剂导致皮肤对激素产生依赖,是其发病的原因,该病的皮肤损害及临床转归与患者使用激素时间的长短、应用的频率、应用激素效力的强弱及个体反应等因... 详细信息

面部糖皮质激素(以下简称激素)依赖性皮炎已成为皮肤科的常见病,长期滥用或误用激素类制剂导致皮肤对激素产生依赖,是其发病的原因,该病的皮肤损害及临床转归与患者使用激素时间的长短、应用的频率、应用激素效力的强弱及个体反应等因素有关。

关键词：皮炎糖皮质激素依赖性面部糖皮质激素

在线全文

学校读者我要写书评

暂无评论

半导体量子点形成临界尺寸的定量预测

物理 2006年第6期35卷 447-450页

作者：吕广宏刘锋美国犹他大学材料科学与工程系

半导体量子点是一类具有显著量子效应的零维量子结构,自组的模型系统,表现为Stranski-Krastanov型生长.其特征为,当超过3—4个Ge单原子层(浸润层)时,则由二维层状生长转变为三维岛状生长.Ge/Si量子点是初期形成的与衬底共格无位错的三维... 详细信息

半导体量子点是一类具有显著量子效应的零维量子结构,自组的模型系统,表现为Stranski-Krastanov型生长.其特征为,当超过3—4个Ge单原子层(浸润层)时,则由二维层状生长转变为三维岛状生长.Ge/Si量子点是初期形成的与衬底共格无位错的三维岛,岛表面由{105}晶面组成.文章作者利用第一性原理计算和介观理论模拟相结合的连续式多尺度(sequential multi-scale)方法,第一次对纯Ge和GeSi合金量子点在Si(001)表面的成核临界尺寸进行了定量的理论预测,同时研究了岛边缘的应力不连续对量子点稳定性的影响,实现了对Ge/Si量子点的形成和稳定性定量的理论研究.

关键词： Ge/Si量子点临界尺寸第一性原理连续体计算模型

MV3和HaCaT细胞接种去表皮真皮组织上构建人工皮肤黑素瘤模型(英文)

在线全文

学校读者我要写书评

暂无评论

中国组织工程研究 2013年第37期17卷 6620-6627页

作者：叶藤卢彬陆洪光贵阳医学院附属医院皮肤科贵州省贵阳市550004

背景:课题组曾成功的用MV3黑素瘤细胞和人角质形成细胞体外重建黑素瘤三维模型。目的:利用MV3黑素瘤细胞和HaCaT细胞结合去表皮的真皮体外重建黑素瘤模型。方法:MV3黑素瘤细胞和HaCaT细胞按照不同比例混合接种于人去表皮的真皮组织上,... 详细信息

背景:课题组曾成功的用MV3黑素瘤细胞和人角质形成细胞体外重建黑素瘤三维模型。目的:利用MV3黑素瘤细胞和HaCaT细胞结合去表皮的真皮体外重建黑素瘤模型。方法:MV3黑素瘤细胞和HaCaT细胞按照不同比例混合接种于人去表皮的真皮组织上,采用液下培养和空气-液面培养相结合技术进行培养,体外构建组织工程皮肤模型。对所构建的皮肤黑素瘤模型进行常规切片免疫组化观察。结果与结论:苏木精-伊红染色显示MV3黑素瘤细胞在去表皮真皮表面成层分布或形成瘤灶,HaCaT细胞和瘤细胞混合生长,形成典型的表皮样结构。部分瘤细胞浸润到去表皮真皮浅层或内部,成瘤灶分布。CK10、CK-pan和S-100免疫组化染色显示阳性。随着MV3∶HaCaT细胞比例的增高,CK10,CK-pan由表层逐渐下移,由层状分布变为团块状分布,S-100蛋白染色则分层逐渐明显,部分区域成瘤状分布。结果可见利用MV3黑素瘤细胞和HaCaT细胞结合去表皮真皮体外可以构建皮肤黑素瘤模型。

关键词：皮肤黑素瘤皮肤模型人工皮肤 T细胞接种真皮组织表皮角质形成细胞种子细胞