Edaravone attenuates ischemia-reperfusion injury by inhibiting oxidative stress in a canine lung transplantation model

作     者：XU Jin-zhi SHEN Bao-zhong LI Ye ZHANG Tong XU Wan-hai LIU Xiao-wei LU Hong-guang 

作者机构：Department of Thoracic Surgery Fourth Affiliated Hospital of Harbin Medical University Harbin Heilongjiang 150001 China Department of Medical Imaging Fourth Affiliated Hospital of Harbin Medical University Harbin Heilongjiang 150001 China 

出 版 物：《Chinese Medical Journal》 (中华医学杂志（英文版）)

年 卷 期：2008年第121卷第16期

页      面：1583-1587页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 100602[医学-中西医结合临床] 10[医学] 

主　　题：edaravone ischemia-reperfusion injury lung transplantation oxidative stress reactive oxygen species 

摘      要：Background Previous reports have confirmed that edaravone has protective effects against ischemia-reperfusion （IR） injury of many organs. In this study, we investigated the effect of edaravone on preventing IR injury of the lung in a canine lung transplantation model. Methods Twelve weight-matched pairs of random-bred dogs were randomized into two groups. Within each pair, one dog served as donor and the other as recipient. In the study group, prostaglandin EI（PGE1）（8 μg/kg） was injected into the donor pulmonary artery （PA） before occlusion and the donor lungs were flushed with 1.0 L of LPD solution containing edaravone （10 mg/kg） and stored in the same LPD solution at a temperature of 1℃for 8 hours. The left single lung transplantation was then performed and recipients received intravenous injection with edaravone （10 mg/kg） at the onset of reperfusion. In the control group, edaravone was substituted by the same volume of sterile saline solution. Another six dogs were obtained as normal control group in which left lungs were dissected after thoracotomy without an IR injury. One hour after reperfusion, or after dissection of the left lung, the right lung was excluded from perfusion and ventilation after which, cardiopulmonary parameters were measured. Wet/dry ratios, malondialdehyde （MDA） and myeloperoxidase （MPO） levels were assessed and histological analysis of lung tissue performed at the same time. Results All animals survived until the end of the experiment. The study group showed significantly decreased wet/dry ratios （treated： （74.1±4.2）% vs control： （86.8±5.2）%, P 〈0.01）, MDA levels （treated： 0.50±0.08 vs control： 0.88±0.15, P 〈0.01） and MPO activity （treated： 0.23±0.05 vs control： 0.43±0.07, P 〈0.01） compared to the control group two hours after occlusion of the right side. In the control group, pulmonary vascular resistance （PVR） was increased markedly and arterial oxygen partial pressure deteriorated significantly after exclusion of the right side compare