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Investigation of the biological roles of autophagy in appressorium morphogenesis in Magnaporthe oryzae

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2008年第10期9卷 793-796页

作者： Xiao-hong liu Fu-cheng LIN State Key Laboratory for Rice Biology Biotechnology Institute Zhejiang University Hangzhou 310029 China

Magnaporthe oryzae has been used as a primary model organism for investigating fungus-plant interaction. Many researches focused on molecular mechanisms of appressorium formation to restrain this fungal pathogen. Autophagy is a very high conserved process in eukaryotic cells. Recently, autophagy has been considered as a key process in development and differentia-tion in M. oryzae. In this report, we present and discuss the current state of our knowledge on gene expression in appressorium formation and the progress in autophagy of rice blast fungi.

关键词： Magnaporthe oryzae Appressorium Gene expression Autophagy

Alleviation of ischemia/reperfusion injury in ob/ob mice by inhibiting UCP-2 expression in fatty liver

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World Journal of Gastroenterology 2008年第4期14卷 590-594页

作者： Chi-Dan Wan Chun-You Wang Tao liu Rui cheng hong-Bo Wang Department of General Surgery Union HospitalTongji Medical College Huazhong University of Science andTechnology Wuhan 430022 Hubei Province China

AIM： To investigate the protective effect of target suppression of uncoupling protein-2 （UCP-2） on ischemia/ reperfusion （I/R） injury in fatty liver in ob/ob mice. METHODS： Plasmids suppressing UCP-2 expression were constructed, and transfected into fatty liver cells cultured in vitro and the ob/ob mouse I/R injury model. Serum tumor necrosis factor （TNF）-α levels, UCP-2 mRNA expression, alanine aminotransferase （ALT） levels in ob/ob mice were tested, and the pathological changes in fatty liver were observed in experimental and control groups. RESULTS： In ob/ob mouse I/R models, serum TNF-α levels were significantly higher than in normal controls. After the plasmids were transfected into the cultured cells and animal models, expression of UCP-2 rnRNA was significantly reduced as compared with that in the control group （2^1.56± 0.15 vs 2^-0.45± 0.15, p 〈 0.05）. In ob/ob mouse models, in which expression of UCP-2 was suppressed, serum ALT levels were significantly lower than those of other groups, and pathological analysis revealed that injury of liver tissues was significantly alleviated. CONCLUSION： The target suppression of UCP-2 expression in fatty liver can alleviate the I/R injury in the ob/ob mice.

关键词： ob/ob mice Fatty liver Uncoupling protein-2 Ischemia/reperfusion injury

Capillary gas chromatographic determination of dimethachlon residues in fresh tobacco leaves and cut-tobacco

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2007年第4期8卷 272-276页

作者： liu hong-cheng LI Qi-wan TANG Li-bin Supervision and Testing Center for Farm Product Quality Ministry of Agriculture （Kunming） Yunnan Academy of Agriculture Science Kunming 650223 China Kunming Institute of Physics Kunming 650223 China

Simple procedures for extraction and chromatographic determination of dimethachlon residues in fresh tobacco leaves and cut-tobacco are *** determination was carried out by capillary gas chromatography(GC) with electron capture detection(ECD) and confirmed by *** mean recoveries and relative standard deviation(RSD) were 93.2%～112.9% and 3.5%～6.7%,respectively at levels ranging from 0.01 to 0.1 mg/*** limit of determination was 0.001 mg/*** samples in routine check were successfully analyzed using the proposed method.

关键词： Dimethachlon residues Tobacco Gas chromatography (GC)

Application Research of “3+1” Mode for Birth Defects Monitoring

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Journal of Reproduction and Contraception 2008年第2期19卷 119-126页

作者： hong liu cheng-liang XIONG Institute for Family Planning Research Tongji Medical College Huazhong University of Science and Technology Wuhan 400030 China

Objective To explore the ＂3＋1＂ monitoring mode for birth defects and quality control measures based on the population, and to obtain the related information data for birth defects.Methods With the community population as the basis, adopting the unified monitoring scheme dominant by the leadership and administration of government, with districts （counties） as the monitoring sites, the ＂3＋1 ＂ monitoring mode for birth defects was based on a complete monitoring team with the combination of villages/residents＇ committees, townships （towns）, counties （districts） and the municipality. Demonstration research was carried out in the pilot districts/counties in Chongqing City.Results Birth defects population monitoring system based on population and family planning management and service network was established, and during 2005 and *** research was carried out for the monitoring methods among birth defects population in the pilot districts （counties）, obtaining the relevant information in regional birth defects, with a monitoring coverage of over 99%. Conclusion Fully utilizing the birth management functions of Population and Famlty Planning System and the advantages of service networks, long term, dynamic birth defects monitoring system based on community population was established, with the integration of birth defects monitoring and regular reproductive health services, obtaining overall birth defects occurrence information in details, providing scientific basis for the government to formulate scientific, practical, economic and effective birth defects intervention policy, so as to improve the quality of the population.

关键词： birth defects monitoring mode

Highly Efficient and Economical Baculovirus Expression System for Preparing Human Papillomavirus Type16 Virus-like Particle

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Acta Biochimica et Biophysica Sinica 2004年第8期36卷 548-552页

作者： Jin ZHENG, Jun MA, Xiao-Feng YANG, hong-Li liu, hong-Wei cheng, Lu-Sheng SI, and Yi-Li WANGThe Key Laboratory of Biomedical Information Engineering of Ministry of Education, Institute of Cancer Research,School of Life Science and Technology, Xi’an Jiaotong University, Xi’an 710061, China The Key Laboratory of Biomedical Information Engineering of Ministry of Education Institute of Cancer Research School of Life Science and TechnologyXi'an Jiaotong University Xi'an 710061 China

To improve the existing human papillomavirus type16 (HPV16) virus-like particle (VLP) preparation, a highly efficient, economical and timesaving system was established. Sf-9 cells were infected with recombinant baculovirus containing the target gene encoding HPV16L1 protein with 6×His tag, and harvested 72 h postinfection (p.i.) at 27 °C. The ProBondTM purification system was used for protein purification. The molecular weight of expressed HPV16L1 protein was 58 kD as revealed by SDS-PAGE, and confirmed by Western blot. The purity of denatured and native HPVL1 proteins that were prepared were 91.9% and 71.5%, respectively, which corresponded to a yield of 2.26 mg denatured protein and 1.84 mg native protein per 2×107 cells. The proteins were further analyzed by mouse erythrocyte hemagglutination assay and hemagglutination inhibition assay, and there effects on VLP formation were also visualized by transmission electron microscopy. Results showed that the native protein purified was biologically active as natural HPVL1 protein, inducing the murine erythrocyte agglutination and VLP formation. In addition, the purified recombinant HPV16L1 native protein with 6×His tag could self-assemble into virions in vitro. Hopefully, the present expression and purification system is promising to be convenient, timesaving and economical for preparation of HPV16 VLP vaccine.

关键词： human papillomavirus HPV16L1 protein purification virus-like particle (VLP)

Synchronous left atrial myxoma and adenosquamous lung carcinoma

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Chinese Medical Journal 2013年第15期126卷 2992-2993页

作者： liu hong-cheng CHEN Xiao-feng YU Yu-ming JIANG Ge-ning Tongji Univ Dept Thorac Surg Shanghai Pulm Hosp Sch Med Shanghai 200433 Peoples R China

Atrial myxomas account for 40%-50% of primary cardiac tumors and as such are the most common primary heart tumors. Because prolapsing myxomas cause the intermittent obstruction of the valvular outflow tract during diastole, the signs and symptoms of left atrial myxoma resemble mitral stenosis. Myxoma may lead to systemic thromboembolic events from tumor fragmentation. In addition, lung carcinoma is a common tumor with highly heterogeneous malignancies.

关键词： left atrial myxoma lung carcinoma synchronous occurrence simultaneous resection

Tailoring Light–Matter Interactions in Overcoupled Resonator for Biomolecule Recognition and Detection

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Nano-Micro Letters 2025年第1期17卷 262-280页

作者： Dongxiao Li hong Zhou Zhihao Ren cheng Xu chengkuo Lee Department of Electrical and Computer Engineering National University of SingaporeSingapore 117583Singapore Center for Intelligent Sensors and MEMS(CISM) National University of SingaporeSingapore 117608Singapore

Plasmonic nanoantennas provide unique opportunities for precise control of light–matter coupling in surface-enhanced infrared absorption(SEIRA)spectroscopy,but most of the resonant systems realized so far suffer from the obstacles of low sensitivity,narrow bandwidth,and asymmetric Fano resonance ***,we demonstrated an overcoupled resonator with a high plasmon-molecule coupling coefficient(μ)(OC-Hμresonator)by precisely controlling the radiation loss channel,the resonator-oscillator coupling channel,and the frequency detuning *** observed a strong dependence of the sensing performance on the coupling state,and demonstrated that OC-Hμresonator has excellent sensing properties of ultra-sensitive(7.25%nm^(−1)),ultra-broadband(3–10μm),and immune asymmetric Fano *** characteristics represent a breakthrough in SEIRA technology and lay the foundation for specific recognition of biomolecules,trace detection,and protein secondary structure analysis using a single array(array size is 100×100μm^(2)).In addition,with the assistance of machine learning,mixture classification,concentration prediction and spectral reconstruction were achieved with the highest accuracy of 100%.Finally,we demonstrated the potential of OC-Hμresonator for SARS-CoV-2 *** findings will promote the wider application of SEIRA technology,while providing new ideas for other enhanced spectroscopy technologies,quantum photonics and studying light–matter interactions.

关键词： Plasmonic nanoantennas Light-matter interaction Surface-enhanced infrared absorption Overcoupled Biosensing

Three-dimensional fracture of UO2 ceramic pellets by phase field modeling

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 169-180页

作者： Wei Xiong Xuan Ye hongzhang cheng Xiaoming liu Institute of Nuclear and New Energy Technology Collaborative Innovation Center of Advanced Nuclear Energy Technology Key Laboratory of Advanced Reactor Engineering and Safety of Ministry of Education Tsinghua University State Key Laboratory of Nonlinear Mechanics Institute of Mechanics Chinese Academy of Sciences School of Engineering Science University of Chinese Academy of Sciences

Fracture is the primary failure type for the UO2ceramic pellets,which are operated under irradiation and thermal processes,and physically confined by the *** this work,by combining closely coupled physical processes of heat conduction,heat transfer,and mechanical deformation with the cohesive phase field framework,we proposed a high-precision modeling of quasistatic cracking of three-dimensional （3D） UO2ceramic fuel pellets under high temperature and *** simulation results agree well with the experimental results,indicating that the proposed method has the potential to capture 3D crack modes in the interested time ***,we studied the relation of the 3D fracture patterns of fuel pellets with the peak power *** was found that the power level plays a critical role in determining the competition between radial and circumferential cracks,as well as transverse penetrating cracks.

关键词： phase field method nuclear fuel pellet coupled thermo-mechanical model thermal fracture

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NICU婴儿培养箱集中管理策略

中国医疗器械杂志 2005年第5期29卷 371-373页

作者：刘宏程山西省儿童医院急救中心山西太原030013

婴儿培养箱的使用和管理存在诸多的问题,其中清洁消毒的问题尤为突出。通过对NlCU婴儿培养箱的集中管理,探索出一条针对临床行之有效的策略。临床工程的介入,使婴儿培养箱在维修、维护、保养、清洁、培训等方面更加合理。科学的管理可... 详细信息

婴儿培养箱的使用和管理存在诸多的问题,其中清洁消毒的问题尤为突出。通过对NlCU婴儿培养箱的集中管理,探索出一条针对临床行之有效的策略。临床工程的介入,使婴儿培养箱在维修、维护、保养、清洁、培训等方面更加合理。科学的管理可优质服务于患儿,取得真正满意的效果。

关键词：婴儿培养箱医学工程早产儿