Alleviation of ischemia/reperfusion injury in ob/ob mice by inhibiting UCP-2 expression in fatty liver
Alleviation of ischemia/reperfusion injury in ob/ob mice by inhibiting UCP-2 expression in fatty liver作者机构：Department of General Surgery Union HospitalTongji Medical College Huazhong University of Science andTechnology Wuhan 430022 Hubei Province China
出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)
年 卷 期：2008年第14卷第4期
页 面：590-594页
核心收录：
学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学]
基 金：Grant from the National Natural Science Foundation of China No. 30571764
主 题：ob/ob mice Fatty liver Uncoupling protein-2 Ischemia/reperfusion injury
摘 要：AIM： To investigate the protective effect of target suppression of uncoupling protein-2 （UCP-2） on ischemia/ reperfusion （I/R） injury in fatty liver in ob/ob mice. METHODS： Plasmids suppressing UCP-2 expression were constructed, and transfected into fatty liver cells cultured in vitro and the ob/ob mouse I/R injury model. Serum tumor necrosis factor （TNF）-α levels, UCP-2 mRNA expression, alanine aminotransferase （ALT） levels in ob/ob mice were tested, and the pathological changes in fatty liver were observed in experimental and control groups. RESULTS： In ob/ob mouse I/R models, serum TNF-α levels were significantly higher than in normal controls. After the plasmids were transfected into the cultured cells and animal models, expression of UCP-2 rnRNA was significantly reduced as compared with that in the control group （2^1.56± 0.15 vs 2^-0.45± 0.15, p 〈 0.05）. In ob/ob mouse models, in which expression of UCP-2 was suppressed, serum ALT levels were significantly lower than those of other groups, and pathological analysis revealed that injury of liver tissues was significantly alleviated. CONCLUSION： The target suppression of UCP-2 expression in fatty liver can alleviate the I/R injury in the ob/ob mice.
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