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CTNNAL1 involves in maintaining the airway integrity of 16HBEC

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CTNNAL1 involves in maintaining the airway integrity of 16HB...

中国生理学会第24届全国会员代表大会暨生理学学术大会

作者：刘彩霞 XQ.Qin hj.liu C.liu ML.Tan Y.Xiang School of Basic Medicine Central South University

Introduction:Our previous study indicated anα-catenin-related protein,catenin alpha-like 1（CTNNAL1）was downregulated in asthma patients and animal ***1 was increased on wound edges of human bronchial epithelial cell（HBEC）,which indicating that it might involve in maintaining the airway integrity of ***:To explore effects of CTNNAL1 on the airway integrity of ***:Overexpression and silenced-plasmid were constructed and transfected into *** efficiency of recombinant plasmid was identified by q PCR and flow cytometry（FCM）.Cell cycle and the mean fluorescence intensity of Ed U were detected by *** matrix adhesion rate of HBEC on rat-tail collagen was detected by *** adhesion molecules（E-cadherin,Integrinβ1 and Integrinβ4）were observed by q ***:CTNNAL1 was increased（189.7%）and decreased（80%）in recombinant plasmid transfected ***1 increased（G2+G1）/G1（33.64%）and the mean fluorescence intensity of Ed U（43.35%）.CTNNAL1 promoted the rate of the matrix adhesion with HBEC（11.26%）and inhibited the decline extent under the stress of ozone from 13.03%to 4.40%.The expression of E-cadherin,Integrinβ1 and Integrinβ4 were increased（114.85%,64.91%and 77.77%）and the declining extent induced by ozone could be weakened from 20.08%,40.61%and 16.88%to 1.83%,15.62%and13.45%.Discussion:CTNNAL1 could promote the matrix adhesion of HBECs and increase the expression of structural adhesion ***1 could alleviate ozone-induced injury in the airway integrity of HBEC.

关键词： CTNNAL1 airway integrity human bronchial epithelial cell

Flexible Ferroelectric Memory Based on Oxide Heteroepitaxy

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Flexible Ferroelectric Memory Based on Oxide Heteroepitaxy

第十四届全国物理力学学术会议

作者：姜杰 HSIEH Y H DO T H liu Q L ZHU X J liu hj MA C H CHU Y H ZHOU Y C School of Materials Science and Engineering Xiangtan University Department of Materials Science and Engineering National Chiao Tung University Institute of Physics Academia Sinica Department of Materials Science and Engineering National Chung Hsing University Institute of Electronics Engineering National Tsing Hua University

The emerging technological demands for flexible electronic devices have compelled researchers to look beyond the current silicon-based electronics.A flexible version of traditional thin lead zirconium titanate(PbZrTiO,PZT)based ferroelectric random access memory(FeRAM)on silicon shows record performance in flexible ***,fabrication of devices

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A Tumor Vascular-Targeted Interlocking Trimodal Nanosystem That Induces and Exploits Hypoxia

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A Tumor Vascular-Targeted Interlocking Trimodal Nanosystem T...

首届东方药理论坛暨2018年全国心脑血管药理学术会议、上海市药理学会第十九届学术年会

作者： Luan X Guan YY liu hj Lu Q Zhao M Sun D Lovell JF Sun P Chen HZ Fang C Hongqiao International Institute of Medicine Shanghai Tongren Hospital and Department of Pharmacology and Chemical Biology Institute of Medical SciencesShanghai Jiao Tong University School of Medicine (SJTU-SM) Department of Pharmaceutical Sciences College of PharmacyUniversity of Michigan Department of Pharmacy Ruijin HospitalSJTU-SM Department of Pharmacy Shanghai University of Medicine and Health Sciences Department of Biomedical Engineering University at BuffaloState University of New York Department of General Surgery Shanghai Tongren HospitalSJTU-SM

Vascular-targeted photodynamic therapy(VTP) is a recently approved strategy for treating solid ***,the exacerbated hypoxic stress makes tumor eradication challenging with such a single modality ***,a new graphene oxide(GO)-based nanosystem for rationally designed,interlocking trimodal cancer therapy that enables VTP using photosensitizer verteporfin(VP)(1) with codelivery of banoxantrone dihydrochloride(AQ4 N)(2),a hypoxia-activated prodrug(HAP),and HIF-1α siRNA(siHIF-1α)(3) is *** VTP-induced aggravated hypoxia is highly favorable for AQ4 N activation into AQ4(a topoisomerase II inhibitor) for ***,the hypoxia-induced HIF-la acts as a "hidden brake,"through downregulating CYP450(the dominant HAP-activating reductases),to substantially hinder AQ4 N ***-la is rationally adopted to suppress the HIF-1α expression upon hypoxia and further enhance AQ4 N *** trimodal nanosystem significantly delays the growth of PC-3 tumors in vivo compared to the control nanoparticles carrying VP,AQ4 N,or siHIF-la alone or their pairwise *** multimodal nanoparticle design presents,the first example exploiting VTP to actively induce hypoxia for enhanced HAP *** is also revealed that HAP activation is still insufficient under hypoxia due to the hidden downregulation of the HAP-activating reductases(CYP450),and this can be well overcome by GO nanoparticle-mediated siHIF-la intervention.

关键词： A Tumor Vascular-Targeted Interlocking Trimodal Nanosystem That Induces and Exploits Hypoxia VTP

肝细胞生长因子诱导前软骨干细胞向软骨细胞的分化

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中国组织工程研究与临床康复 2007年第27期11卷 5299-5302页

作者：饶耀剑曹向阳刘慧娟河南省洛阳正骨医院脊柱外科河南省洛阳市471000

目的:探讨肝细胞生长因子诱导前软骨干细胞向软骨细胞分化的机制。方法:实验于2005-12/2006-03在河南省正骨研究院完成。①实验材料:普通级新生兔10只,平均体质量3kg。②实验干预:采用免疫磁珠分离技术分离新生兔长骨干骺端前软骨干细... 详细信息

目的:探讨肝细胞生长因子诱导前软骨干细胞向软骨细胞分化的机制。方法:实验于2005-12/2006-03在河南省正骨研究院完成。①实验材料:普通级新生兔10只,平均体质量3kg。②实验干预:采用免疫磁珠分离技术分离新生兔长骨干骺端前软骨干细胞。利用肝细胞生长因子(浓度分别为0.5,1,2,4μg/L)作用前软骨干细胞;另以2μg/L肝细胞生长因子作用前软骨干细胞,对照用相同剂量的培养基,检测前软骨干细胞Ⅱ型胶原及Ⅱ型胶原mRNA的表达。③实验评估:采用光学显微镜观察前软骨干细胞形态及生长情况;四甲基偶氮唑盐比色法检测前软骨干细胞的增殖;免疫荧光检测Ⅱ型胶原表达;反转录-聚合酶链反应检测Ⅱ型胶原mRNA的表达。结果:①前软骨干细胞形态及生长情况:分离纯化的前软骨干细胞贴壁稳定,在光学显微镜下呈多角和梭形,生长旺盛,曲光度好。②前软骨干细胞增殖情况:细胞增殖率上升,在24,48,72h内呈时间浓度依赖。③Ⅱ型胶原表达:肝细胞生长因子作用第5天前软骨干细胞出现Ⅱ型胶原蛋白的表达。④Ⅱ型胶原mRNA的表达:在肝细胞生长因子刺激3d开始出现表达,并且5d,7d逐渐增加。结论:肝细胞生长因子作用前软骨干细胞后,出现软骨特征标志抗原Ⅱ型胶原的表达,细胞增殖活性上升,表明肝细胞生长因子能诱导前软骨干细胞向软骨细胞方向分化。其机制可能是前软骨干细胞也存在肝细胞生长因子受体。

关键词：肝细胞生长因子干细胞软骨/细胞学细胞分化组织构建

Synthesis of chitosan-based grafting magnetic flocculants for flocculation of kaolin suspensions

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Journal of Environmental Sciences 2024年第5期139卷 193-205页

作者： Chuang liu Xiaoyu Wang Sicong Du Wenyan Liang Beijing Key Lab for Source Control Technology of Water Pollution/Engineering Research Center for Water Pollution Source Control&Eco-remediation/College of Environmental Science and Engineering Beijing Forestry UniversityBeijing 100083China

A series of novel chitosan-based magnetic flocculants FS@CTS-P(AM-DMC)was prepared by molecular structure *** characterization results showed that FS@CTS-P(AM-DMC)had a uniform size of about 21.46 nm,featuring a typical core-shell structure,and the average coating layer thickness of CTS-P(AM-DMC)was about 5.03 ***@CTS-P(AM-DMC)exhibited excellent flocculation performance for kaolin suspension,achieved 92.54% turbidity removal efficiency under dosage of 150 mg/L,pH 7.0,even at high turbidity(2000 NTU)with a removal efficiency of 96.96%.The flocculation mechanism was revealed to be dominated by charge neutralization under acidic and neutral conditions,while adsorption and bridging effects play an important role in alkaline *** properties of magnetic aggregates during flocculation,breakage,and regeneration were studied at different pH levels and *** the process of magnetophoretic,magnetic particles collide and adsorb with kaolin particles continuously due to magnetic and electrostatic attraction,transform into magnetic chain clusters,and then further form three-dimensional network magnetic aggregates that can capture free kaolin particles and other chain *** image velocimetry confirmed the formation of eddy current of magnetic flocs and experienced three stages:acceleration,stabilization,and deceleration.

关键词： Magnetic flocculation Chitosan Fe_(3)O_(4) Graft copolymerization PIV

Integrated Combination Treatment Using a “Smart” Chemotherapy and MicroRNA Delivery System Improves Outcomes in an Orthotopic Colorectal Cancer Model

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Integrated Combination Treatment Using a “Smart” Chemother...

首届东方药理论坛暨2018年全国心脑血管药理学术会议、上海市药理学会第十九届学术年会

作者： liu hj Luan X Feng HY Dong X Yang SC Chen ZJ Cai QY Lu Q Zhang Y Sun P Zhao M Chen HZ Lovell JF Fang C Hongqiao International Institute of Medicine Shanghai Tongren Hospital and Department of Pharmacology and Chemical Biology Institute of Medical SciencesShanghai Jiao Tong University School of Medicine (SJTU-SM) Department of General Surgery Shanghai Tongren HospitalSJTU-SM Department of Pharmacy Shanghai University of Medicine and Health Sciences Department of Biomedical Engineering University at BuffaloState University of New York

Mesoporous silica nanoparticles(MSN) can load and deliver potentially synergistic anticancer agents such as small molecule cytotoxics(like doxorubicin,DOX) and nucleic acids(like microRNA,miRNA).However,these cargos have different underlying chemical properties so overcoming respective intracellular delivery barriers is a key *** to deliver DOX from MSN frequently employ pH-driven mechanisms that are restricted to the acidic environment of ***,strategies to deliver miRNA make use of approaches that deliberately compromise lysosomal membrane integrity to enable cytosolic delivery of the *** reconcile these two needs(lysosomal delivery of DOX and intracellular delivery of miRNA),a new methodology by "weaving" polyethylenimine on the MSN surface through disulfide bonds to achieve superior delivery of chemotherapy(DOX) and miRNA therapy(using miRNA-145) is ***,an active targeting strategy based on a peptide ligand with affinity to glucose-regulated protein 78(GRP78),a cell surface protein overexpressed in colorectal carcinoma,is *** active targeting approach results in enhanced synergistic antitumor effect both in vitro and in vivo in an orthotopic murine model of colorectal *** together,this work demonstrates the capability and advantages of "smart" MSN delivery systems to deliver anticancer cargo appropriately to targeted cancer cells.

关键词： miRNA Integrated Combination Treatment Using a Smart Chemotherapy and MicroRNA Delivery System Improves Outcomes in an Orthotopic Colorectal Cancer Model

应用3.0T MRI评价缺血性脑血管病与鼻窦炎性病变的关系

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中国组织工程研究与临床康复 2007年第48期11卷 9722-9725页

作者：李晖刘怀军朱清风杨冀萍张强河北医科大学第二医院医学影像科河北省石家庄市050000

目的:应用3.0TMRI从影像学角度探讨缺血性脑血管病与鼻旁窦炎性病变的关系及鼻旁窦炎性病变的MRI表现。方法:①分组及检测:2006-10/2007-08河北医科大学第二医院收治经临床及影像学证实为缺血性脑血管病患者218例为病例组,健康体检者21... 详细信息

目的:应用3.0TMRI从影像学角度探讨缺血性脑血管病与鼻旁窦炎性病变的关系及鼻旁窦炎性病变的MRI表现。方法:①分组及检测:2006-10/2007-08河北医科大学第二医院收治经临床及影像学证实为缺血性脑血管病患者218例为病例组,健康体检者218例为对照组,应用美国GE公司Signa Excite HD 3.0T高场强MR扫描仪常规行MRI及MRA检查。②评估指标:MRI图像观察以轴位T2-FSE、轴位T1-FLAIR序列为主,辅以轴位T2-FLAIR、矢状位T1-FLAIR序列,观察并记录脑实质MRI表现、鼻旁窦有无炎性病变。MRA图像观察以MIP重组后图像为主,辅以MRA源图像,观察并记录颅内血管异常情况。主要观察指标包括额窦、筛窦、蝶窦、上颌窦受累情况,各窦病变类型(黏膜增厚、炎性积液、黏膜下囊肿)及相应的MRI表现。结果:①病例组所有患者前循环及后循环系统血管均存在不同程度的动脉粥样硬化改变,143例患者脑实质出现缺血性病变;对照组脑实质及脑血管均未见异常改变。②病例组鼻旁窦有病变者123人次(56.4%),上颌窦病变96例,筛窦病变58例,蝶窦病变30例,额窦病变15例;对照组鼻旁窦有病变者54人次(24.8%),上颌窦病变8例,筛窦病变34例,蝶窦病变2例,额窦病变4例。病例组鼻旁窦炎性病变明显较对照组多(χ2=45.281,P=0.000)。相关系数C=0.91。结论:缺血性脑血管病患者鼻旁窦炎性病变发病率高,缺血性脑血管病与鼻旁窦炎性病变间有一定的相关性。

关键词：缺血性脑血管病鼻旁窦炎症磁共振成像

Application of CRISPR/Cas12i.3 for targeted mutagenesis in broomcorn millet(Panicum miliaceum L.)

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Journal of Integrative Plant Biology 2024年第8期66卷 1544-1547页

作者： Yuhe Bai Shengnan liu Yan Bai Zhisong Xu Hainan Zhao Haiming Zhao Jinsheng Lai Ya liu Weibin Song State Key Laboratory of Maize Bio-breeding Key Laboratory of Genome Editing Research and ApplicationMinistry of Agriculture and Rural AffairsDepartment of Plant Genetics and BreedingNational Maize Improvement CenterCollege of Agronomy and BiotechnologyChina Agricultural UniversityBeijing 100193China National Agricultural Technology Extension&Service Center Beijing 100125China Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding Beijing Academy of Agriculture and Forestry SciencesBeijing 100097China

As the global human population continues to grow and is predicted to reach 10 billion by 2050,the demand for food volume and quality raises a tremendous challenge(OECD/FAO,2017).The worldwide arable land area is decre... 详细信息

关键词： arable continue raise

WTV2.0:A high-coverage plant volatilomics method with a comprehensive selective ion monitoring acquisition mode

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Molecular Plant 2024年第6期17卷 972-985页

作者： Honglun Yuan Yiding Jiangfang Zhenhuan liu Rongxiu Su Qiao Li Chuanying Fang Sishu Huang Xianqing liu Alisdair R.Fernie Jie Luo School of Breeding and Multiplication(Sanya Institute of Breeding and Multiplication)and College of Tropical Agriculture and Forestry Hainan UniversitySanya Hainan 572025China Yazhouwan National Laboratory(YNL) Sanya Hainan 572025China Department of Molecular Physiology Max-Planck-Institute of Molecular Plant PhysiologyAm Mühlenberg 1Potsdam-Golm 14476Germany

Volatilomics is essential for understanding the biological functions and fragrance contributions of plant ***,the annotation coverage achieved using current untargeted and widely targeted volatomics(WTV)methods has been limited by low sensitivity and/or low acquisition ***,we introduce WTV 2.0,which enabled the construction of a high-coverage library containing 2111 plant volatiles,and report the development of a comprehensive selective ion monitoring(cSIM)acquisition method,including the selection of characteristic qualitative ions with the minimal ion number for each compound and an optimized segmentation method,that can acquire the smallest but sufficient number of ions for most plant volatiles,as well as the automatic qualitative and semi-quantitative analysis of cSIM ***,the library and acquisition method we developed can be self-expanded by incorporating compounds not present in the library,utilizing the obtained cSIM *** showed that WTV 2.0 increases the median signal-to-noise ratio by 7.6-fold compared with the untargeted method,doubled the annotation coverage compared with the untargeted and WTV 1.0 methods in tomato fruit,and led to the discovery of menthofuran as a novel flavor compound in passion *** 2.0 is a Python library with a user-friendly interface and is applicable to profiling of volatiles and primary metabolites in any species.

关键词： volatilomics widely targeted methods GC-MS selective ion monitoring plant volatiles