CTNNAL1 involves in maintaining the airway integrity of 16HBEC
作者单位：School of Basic MedicineCentral South University
会议名称：《中国生理学会第24届全国会员代表大会暨生理学学术大会》
会议日期：2014年
学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学]
基 金：supported by NSFC#81270065 Hunan Provincial Innovation Foundation for Postgraduate(2013zzts070)
关 键 词：CTNNAL1 airway integrity human bronchial epithelial cell
摘 要：Introduction:Our previous study indicated anα-catenin-related protein,catenin alpha-like 1（CTNNAL1）was downregulated in asthma patients and animal ***1 was increased on wound edges of human bronchial epithelial cell（HBEC）,which indicating that it might involve in maintaining the airway integrity of ***:To explore effects of CTNNAL1 on the airway integrity of ***:Overexpression and silenced-plasmid were constructed and transfected into *** efficiency of recombinant plasmid was identified by q PCR and flow cytometry（FCM）.Cell cycle and the mean fluorescence intensity of Ed U were detected by *** matrix adhesion rate of HBEC on rat-tail collagen was detected by *** adhesion molecules（E-cadherin,Integrinβ1 and Integrinβ4）were observed by q ***:CTNNAL1 was increased（189.7%）and decreased（80%）in recombinant plasmid transfected ***1 increased（G2+G1）/G1（33.64%）and the mean fluorescence intensity of Ed U（43.35%）.CTNNAL1 promoted the rate of the matrix adhesion with HBEC（11.26%）and inhibited the decline extent under the stress of ozone from 13.03%to 4.40%.The expression of E-cadherin,Integrinβ1 and Integrinβ4 were increased（114.85%,64.91%and 77.77%）and the declining extent induced by ozone could be weakened from 20.08%,40.61%and 16.88%to 1.83%,15.62%and13.45%.Discussion:CTNNAL1 could promote the matrix adhesion of HBECs and increase the expression of structural adhesion ***1 could alleviate ozone-induced injury in the airway integrity of HBEC.