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Journal of Ocean University of China 2018年第4期17卷 947-956页

作者： WU Xueping FENG Yanwei JIANG Hailin liu xiangquan PAN Ying Key Laboratory of Marine Ecological Restoration Shandong Marine Resource and Environment Research Institute College of Animal Science and Technology Guangxi University Key Laboratory of Protection and Utilization of Marine Resources Guangxi University of Nationalities

Sinonovacula constricta is one of the important economic aquaculture species in China. In this study, we constructed genetic linkage maps of S. constricta based on 300 microsatellite markers derived from RAD-seq using an F1 full-sib family. The female map contained 204 microsatellites assigned to 22 linkage groups, which covered 1529.5 cM with an average interval of 10.3 cM. The male consisted of 187 microsatellites in 19 linkage groups corresponding to the haploid chromosome number(n(28)19), which spanned 1429.3 cM with an average interval of 8.7 cM. The genome coverage was approximately 83.5% and 81.4%, respectively. An integrated map was constructed according to the common markers in parental linkage groups, which had a total length of 1683.8 cM with an average interval of 7.3 cM. The genome coverage of the integrated map was approximately 86.3%. The genetic linkage map would form the foundation for further studies on the quantitative trait loci(QTL), as well as accelerating the breeding process of this species.

关键词： linkage maps Sinonovacula constricta microsatellite marker RAD sequencing

Two-step growth of β-Ga_(2)O_(3) on c-plane sapphire using MOCVD for solar-blind photodetector

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Journal of Semiconductors 2024年第2期45卷 51-56页

作者： Peipei Ma Jun Zheng xiangquan liu Zhi liu Yuhua Zuo Buwen Cheng State Key Laboratory on Integrated Optoelectronics Institute of SemiconductorsChinese Academy of SciencesBeijing 100083China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing 100049China

In this work,a two-step metal organic chemical vapor deposition(MOCVD)method was applied for growingβ-Ga_(2)O_(3) film on c-plane *** buffer layer growth temperature(T_(B))was found at 700℃ and theβ-Ga_(2)O_(3) film with full width at half maximum(FWHM)of 0.66°was achieved.A metal−semiconductor−metal(MSM)solar-blind photodetector(PD)was fabricated based on theβ-Ga_(2)O_(3) *** responsivity of 1422 A/W@254 nm and photo-to-dark current ratio(PDCR)of 10^(6) at 10 V bias were *** detectivity of 2.5×10^(15) Jones proved that the photodetector has outstanding performance in detecting weak ***,the photodetector exhibited superior wavelength selectivity with rejection ratio(R_(250 nm)/R_(400 nm))of *** results indicate that the two-step method is a promising approach for preparation of high-qualityβ-Ga_(2)O_(3)films for high-performance solar-blind photodetectors.

关键词： MOCVD two-step growth β-Ga_(2)O_(3) solar-blind photodetector responsivity

Construction of a Normalized Full-Length cDNA Library of Cephalopod Amphioctopus fangsiao and Development of Microsatellite Markers

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Journal of Ocean University of China 2017年第5期16卷 897-904页

作者： FENG Yanwei liu Wenfen XU Xin YANG Jianmin WANG Weijun WEI Xiumei liu xiangquan SUN Guohua Key Laboratory of Marine Ecological Restoration Shan Dong Marine Resource and Environment Research Institute College of Fisheries and Life Science Shanghai Ocean University Key Laboratory of Marine Genetics and Breeding of Ministry of Education Ocean University of China

Amphioctopus fangsiao is one of the most economically important species and has been considered to be a candidate for aquaculture. In order to facilitate its fine-scale genetic analyses, we constructed a normalized full-length library successfully and developed a set of microsatellite markers in this study. The normalized full-length library had a storage capacity of 6.9×105 independent clones. The recombination efficiency was 95% and the average size of inserted fragments was longer than 1000 bp. A total of 3440 high quality ESTs were obtained, which were assembled into 1803 unigenes. Of these unigenes, 450(25%) were assigned into 33 Gene Ontology terms, 576(31.9%) into 153 Kyoto Encyclopedia of Genes and Genomes pathways, and 275(15.3%) into 22 Clusters of Orthologous Groups. Seventy-six polymorphic microsatellite markers were identified. The number of alleles per locus ranged from 4 to 17, and the observed and expected heterozygosities varied between 0.167 and 0.967 and between 0.326 and 0.944, respectively. Twelve loci were significantly deviated from Hardy-Weinberg equilibrium after Bonferroni correction and no linkage disequilibrium was found between different loci. This study provided not only a useful resource for the isolation of the functional genes, but also a set of informative microsatellites for the assessment of population structure and conservation genetics of A. fangsiao.

关键词： microsatellite ESTs polymorphic informative normalized length alleles isolation aquaculture linkage

Comparative transcriptome analysis of abalone Haliotis discus hannai with green and gray egg colors

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Journal of Oceanology and Limnology 2021年第1期39卷 350-361页

作者： Yanwei FENG Bo YUAN Xu JIANG xiangquan liu Zan LI Guohua SUN Xiaohui XU School of Agriculture Ludong UniversityYantai 264025China Shandong Provincial Key Laboratory of Restoration for Marine Ecology Shandong Marine Resource and Environment Research InstituteYantai 264006China National Demonstration Center for Experimental Fisheries Science Education Shanghai Ocean UniversityShanghai 201306China Shanghai Engineering Research Center of Aquaculture Shanghai Ocean UniversityShanghai 201306China Shandong Rice Research Institute Shandong Academy of Agricultural SciencesJinan 250100China

Haliotis discus hannai is an important marine economic species in *** egg color was found to be associated with economic traits,which provides a new idea for ***,the molecular mechanism of the egg-color formation has not been ***,the pigment composition and comparative transcriptome analyses of *** hannai with green and gray egg color were conducted using high-performance liquid chromatography(HPLC)and RNA-Seq *** show that individuals with green and gray eggs both possess the *** existed in gray-egged individuals,but not in green-egged *** transcriptome analysis,272310 unigenes were received from 461162 transcripts with a mean length of 985 bp and N50 of 1524 bp,respectively.A total of 185 unigenes were identifi ed as diff erentially expressed genes(DEGs).The DEGs involved in“fl avin-containing compound metabolic process”,“melanosome”,“glutathione metabolism”,and“cytochrome b6f complex”were likely related to the formation of the egg *** results provide foundational information for the functional analysis of egg-color related genes and are benefi cial to the selective breeding of *** hannai.

关键词： Haliotis discus hannai egg color high-performance liquid chromatography transcriptome diff erentially expressed genes

980 nm Near-Infrared Light-Emitting Diode Using All-Inorganic Perovskite Nanocrystals Doped with Ytterbium Ions

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Tsinghua Science and Technology 2024年第1期29卷 207-215页

作者： Zhenglan Ye Taoran liu Dan Chen Yazhou Yang Jiayi Li Yaqing Pang xiangquan liu Yuhua Zuo Jun Zheng Zhi liu Buwen Cheng State Key Laboratory on Integrated Optoelectronics Institute of SemiconductorsChinese Academy of SciencesBeijing 100083China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing 100049China Intelligent Network Research Institute Zhejiang LabHangzhou 311100China

All-inorganic perovskite(CsPbX3)nanocrystals(NCs)have recently been widely investigated as versatile solution-processable light-emitting *** to its wide-bandgap nature,the all-inorganic perovskite NC Light-Emitting Diode(LED)is limited to the visible region(400-700 nm).A particularly difficult challenge lies in the practical application of perovskite NCs in the infrared-spectrum *** this work,a 980 nm NIR all-inorganic perovskite NC LED is demonstrated,which is based on an efficient energy transfer from wide-bandgap materials(CsPbCl3 NCs)to ytterbium ions(Yb3+)as an NIR emitter doped in perovskite *** optimized CsPbCl3 NC with 15 mol%Yb3+doping concentration has the strongest 980 nm photoluminescence(PL)peak,with a PL quantum yield of 63%.An inverted perovskite NC LED is fabricated with the structure of ITO/PEDOT:PSS/poly-TPD/CsPbCl3:15 mol%Yb3+NCs/TPBi/LiF/*** LED has an External Quantum Efficiency(EQE)of 0.2%,a Full Width at Half Maximum(FWHM)of 47 nm,and a maximum luminescence of 182 cd/m?.The introduction of Yb3+doping in perovskite NCs makes it possible to expand its working wavelength to near-infrared band for next-generation light sources and shows potential applications for optoelectronic integration.

关键词： perovskite nanocrystals rare-earth doping Light-Emitting Diode(LED) near-infrared optical interconnection

AFLP analysis revealed differences in genetic diversity of four natural populations of Manila clam (Ruditapes philippinarum) in China

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Acta Oceanologica Sinica 2007年第1期26卷 150-158页

作者： liu xiangquan BAO Zhenmin HU Jingjie WANG Shi ZHAN Aibin liu Hui FANG Jianguang WANG Rucai Laboratory of Marine Genetics and Breeding （ MGB） Ocean University of China Qingdao 266003 China Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Science Qingdao 266071 China

The amplified fragment length polymorphism （AFLP） technology was used to analyze the genetic diversities in four natural populations of Manila clam （ Ruditapes philippinarum）, distributed in four sea areas of China, i.e. , the Bohai Sea, the Huanghai Sea, the East China Sea and the South China Sea. Two hundred and sixty-four AFLP loci were analysed in 195 individuals and revealed high levels of genetic diversity. The percentage of polymorphic loci ranged from 92.13% to 96.06% and the Shannon＇ s information index was from 0.256 8 to 0. 275 6. By analyzing molecular variance （ AMOVA）, it was found that there were high levels of genetic differentiation between populations of Qingdao and the other three sea areas. Cluster analysis by Nei＇ s pairwise distances grouped specimens by geographical origin, except the population of Qingdao. A conclusion can be drawn that there are high genetic diversities in the four natural populations of Manila clam in China and some distinct differences existed among and between the four populations. The results also indicated that human cultivation activities will have great influence on the genetic structure of the population of Qingdao.

关键词： Ruditapes philippinarum genetic diversity geographical population AFLP

75 GHz germanium waveguide photodetector with 64 Gbps data rates utilizing an inductive-gain-peaking technique

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Journal of Semiconductors 2023年第1期44卷 79-84页

作者： Xiuli Li Yupeng Zhu Zhi liu Linzhi Peng xiangquan liu Chaoqun Niu Jun Zheng Yuhua Zuo Buwen Cheng State Key Laboratory on Integrated Optoelectronics Institute of SemiconductorsChinese Academy of SciencesBeijing 100083China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing 100049China

High-performance germanium(Ge)waveguide photodetectors are designed and fabricated utilizing the inductivegain-peaking *** the appropriate integrated inductors,the 3-dB bandwidth of photodetectors is significantly improved owing to the inductive-gain-peaking effect without any compromises to the dark current and optical *** 3-dB bandwidth up to 75 GHz is realized and clear open eye diagrams at 64 Gbps are *** this work,the relationship between the frequency response and large signal transmission characteristics on the integrated inductors of Ge waveguide photodetectors is investigated,which indicates the high-speed performance of photodetectors using the inductive-gainpeaking technique.

关键词： germanium photodetectors inductive-gain-peaking optical interconnection

Construction of a Full-Length cDNA Library of Solen grandis Dunker and Identification of Defense- and Immune-Related Genes

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Journal of Ocean University of China 2014年第1期13卷 169-173页

作者： SUN Guohua liu xiangquan REN Lihua YANG Jianmin WEI Xiumei YANG Jialong Shandong Provincial Key Laboratory of Restoration for Marine Ecology Shandong Marine Fisheries Research Institute Yantai Institute of Coastal Zone Research Chinese Academy of Sciences

The basic genetic characteristics, important functional genes, and entire transcriptome of Solen grandis Dunker were investigated by constructing a full-length cDNA library with the ‘switching mechanism at the 5'-end of the RNA transcript'(SMART) technique. Total RNA was isolated from the immune-relevant tissues, gills and hemocytes, using the Trizol reagent, and cDNA fragments were digested with Sfi I before being ligated to the pBluescript II SK* vector. The cDNA library had a titer of 1048 cfu μL-1 and a storage capacity of 1.05×106 cfu. Approximately 98% of the clones in the library were recombinants, and the fragment lengths of insert cDNA ranged from 0.8 kb to 3.0 kb. A total of 2038 expressed sequence tags were successfully sequenced and clustered into 965 unigenes. BLASTN analysis showed that 240 sequences were highly similar to the known genes(E-value 80%), accounting for 25% of the total unigenes. According to the Gene Ontology, these unigenes were related to several biological processes, including cell structure, signal transport, protein synthesis, transcription, energy metabolism, and immunity. Fifteen of the identified sequences were related to defense and immunity. The full-length cDNA sequence of HSC70 was obtained. The cDNA library of S. grandis provided a useful resource for future researches of functional genomics related to stress tolerance, immunity, and other physiological activities.

关键词： Solen grandis cDNA library EST sequence defense and immune functional gene

Horizontal GeSn/Ge multi-quantum-well ridge waveguide LEDs on silicon substrates

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Photonics Research 2020年第6期8卷 899-903页

作者： LINZHI PENG Xu LI ZHI liu xiangquan liu JUN ZHENG CHUNLAI XUE YUHUA ZUO BUWEN CHENG State Key Laboratory on Integrated Optoelectronics Institute of SemiconductorsChinese Academy of SciencesBeijing 100083China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeiing 100049China Beijing Academy of Quantum Information Sciences Bejing 100193China

A horizontal p-i-n ridge waveguide emitter on a slion(100)substrate with a Gen,g1Sno.c9/Ge multi quantum-well(MQW)active layer was fabricated by molecular beam *** device structure was designed to reduce light absorption of metal electrodes and improve injection ***(EL)at a wavelength of 2160 nm was observed at room *** calculations indicate that the emission peak corresponds well to the direct bandgap transition(nr-mHr).The light output power was about 2.0μW with an injection current density of 200 kA/cm^*** results show that the horizontal GeSn/Ge MQW ridge waveguide emiters have great pros-pects for group-IV light sources.

关键词： waveguide temperature. GeSn

High-speed GeSn resonance cavity enhanced photodetectors for a 50 Gbps Si-based 2 μm band communication system

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Photonics Research 2024年第4期12卷 767-773页

作者： JINLAI CUI JUN ZHENG YUPENG ZHU xiangquan liu YIYANG WU QINXING HUANG YAZHOU YANG ZHIPENG liu ZHI liu YUHUA ZUO BUWEN CHENG Key Laboratory of Optoelectronic Materials and Devices Institute of SemiconductorsChinese Academy of SciencesBeijing 100083China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of SciencesBeijing 100049China

Expanding the optical communication band is one of the most effective methods of overcoming the nonlinear Shannon capacity limit of single *** this study,GeSn resonance cavity enhanced(RCE)photodetectors(PDs)with an active layer Sn component of 9%–10.8%were designed and fabricated on an SOI *** GeSn RCE PDs present a responsivity of 0.49 A/W at 2μm and a 3-dB bandwidth of approximately 40 GHz at 2μ***,Si-based 2μm band optical communication with a transmission rate of 50 Gbps was demonstrated by using a GeSn RCE *** work demonstrates the considerable potential of the Si-based 2μm band photonics in future high-speed and high-capacity optical communication.

关键词： resonance system approximate