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Pyruvate kinase M in germ cells is essential for sperm motility and male fertility but not spermatogenesis

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Asian Journal of Andrology 2024年第2期26卷 212-219页

作者： Gao-Qing Qian xiao-Chen Wang xi Zhang Bin Shen Qiang liu State Key Laboratory of Reproductive Medicine and Offspring Health Nanjing Medical UniversityNanjing 211166China Shanghai Key Laboratory of Reproductive Medicine Department of HistoembryologyGenetics and Developmental BiologyShanghai Jiao Tong University School of MedicineShanghai 200025China State Key Laboratory of Molecular Biology Shanghai Key Laboratory of Molecular AndrologyShanghai Institute of Biochemistry and Cell BiologyCenter for Excellence in Molecular Cell ScienceChinese Academy of SciencesUniversity of Chinese Academy of SciencesShanghai 200031China

Male germ cells employ specific metabolic pathways throughout their developmental *** a previous study,we discovered heightened expression of pyruvate kinase M(PKM),a pivotal glycolytic enzyme,in spermatogonia and *** gain deeper insights into PKM's roles in spermatogenesis,sperm function,and male fertility,we engineered a conditional-knockout mouse model(Pkm-vkO mice)to selectively disrupt the Pkm gene within germ *** maintaining regular testicular histology and sperm morphology,the male Pkm-vko mice were infertility,characterized by significant impairments in sperm motility and adenosine triphosphate(ATP)*** addition,Pkm-null spermatozoa exhibited similar deficits in protein tyrosine phosphorylation linked to capacitation,as well as compromised performance in in vitro fertilization *** conclude,PKM's presence is not obligatory for the entirety of spermatogenesis in male germ cells;however,it emerges as a critical factor influencing sperm motility and overall male fertility.

关键词： glycolysis male fertility pyruvate kinase M sperm motility spermatogenesis

Mutation in the Agrobacterium his I gene enhances transient expression in pepper

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Horticultural Plant Journal 2024年第3期10卷 809-822页

作者： Dan liu Shengnan Zhao Jubin Wang xi Zhang Yingtian Deng Feng Li National Key Laboratory for Germplasm Innovation&Utilization of Horticultural Crops College of Horticulture and Forestry SciencesHuazhong Agricultural UniversityWuhanHubei 430070China

Agrobacterium-mediated plant transformation is widely used in plant genetic ***,its efficiency is limited by plant immunity against *** pepper(Capsicum annuum L.)is an important vegetable that is recalcitrant to Agrobacterium-mediated *** this work,Agrobacterium was found to induce a strong immune response in pepper,which might be the reason for T-DNA being difficult to express in *** Agrobacterium mutant screen was conducted and a point mutation in the hisI gene was identified due to a weak immune response and enhanced transient expression mediated by this Agrobacterium mutant in pepper *** genetic analysis revealed that histidine biosynthesis deficiency caused by mutations in many genes of this pathway led to reduced pepper cell death,presumably due to reduced bacterial ***,mutation analysis of threonine and tryptophan biosynthesis genes showed that the biosynthesis of different amino acids may play different roles in Agrobacterium growth and stimulating the pepper immune *** possible application of Agrobacterium amino acid biosynthesis mutations in plant biology was discussed.

关键词： Capsicum annuum L. Agrobacterium Transient transformation efficiency Mutation Amino acid Immune response

Observation and verification of surface electrical explosion driven by radial-distributed pulsed current in laboratory lightning strike test

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High Voltage 2024年第1期9卷 137-149页

作者： Ruoyu Han Yuchen Cao Yakun liu xi Chen Ting Li Chen Li Pengfei Li State Key Laboratory of Explosion Science and Technology Beijing Institute of TechnologyBeijingChina School of Mechatronical Engineering Beijing Institute of TechnologyBeijingChina Department of Electrical Engineering Shanghai Jiaotong UniversityShanghaiChina Beijing Institute of Astronautical System Engineering BeijingChina Aerospace Research Institute of Materials and Processing Technology BeijingChina

The laboratory lightning test is essential for assessing the effectiveness of lightning strike protection(LSP).Particularly,direct lightning strike damage can be performed with pulsed current injection into the *** paper focuses on the dynamic process of arc plasma and shock wave behaviour in the vicinity of the‘strike’point.A rod-plate discharge load is built for testing aluminium and coated plate under 40-kA-level pulsed *** visualisation of the luminous discharge plasma and its flow field via high-speed photography(from different angles)is meticulously designed and implemented,synchronised with electro-physical *** results indicate some new mechanisms for lightning strike damage,apart from the impulse heat loading from the thermal *** transient current injection through the arc root concentrates on a thin skin layer(skin-depth effect),with the radial-attenuated current density,driving asynchronously electrical explosions on the plate *** inhomogeneous Joule heating of the plate leads to outwardly propagating phase transition and shock wave along the conductive *** addition,the electro-thermal instability is observed and regarded as the seed of irregular erosion *** information reveals two different plasma states of main discharge arc channel and adjacent surface electrical *** correspondence of the physical mechanism of electrical explosion and optical radiation is *** images for different regions depict erosion characteristics and summarise influencing factors,further confirming the mechanism *** research clarifies the role of skin-depth effect in transaction arc erosion for electrode,complements the electrical explosion theory with unevenly distributed current and helps optimise strategies of LSP.

关键词： electrical lightning radial

Rational Design of Ruddlesden-Popper Perovskite Ferrites as Air Electrode for Highly Active and Durable Reversible Protonic Ceramic Cells

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Nano-Micro Letters 2024年第9期16卷 308-324页

作者： Na Yu Idris Temitope Bello xi Chen Tong liu Zheng Li Yufei Song Meng Ni Department of Building and Real Estate Research Institute for Sustainable Urban Development(RISUD)and Research Institute for Smart Energy(RISE)The Hong Kong Polytechnic UniversityHung HomKowloonHong KongPeople’s Republic of China The Hong Kong Polytechnic University Shenzhen Research Institute Shenzhen 518057GuangdongPeople’s Republic of China Department of Mechanical and Aerospace Engineering The Hong Kong University of Science and TechnologyClear Water BayHong KongPeople’s Republic of China

Reversible protonic ceramic cells(RePCCs)hold promise for efficient energy storage,but their practicality is hindered by a lack of high-performance air electrode ***-Popper perovskite Sr_(3)Fe_(2)O_(7−δ)(SF)exhibits superior proton uptake and rapid ionic conduction,boosting ***,excessive proton uptake during RePCC operation degrades SF’s crystal structure,impacting *** study introduces a novel A/B-sites co-substitution strategy for modifying air electrodes,incorporating Sr-deficiency and Nb-substitution to create Sr_(2.8)Fe_(1.8)Nb_(0.2)O_(7−δ)(D-SFN).Nb stabilizes SF’s crystal,curbing excessive phase formation,and Sr-deficiency boosts oxygen vacancy concentration,optimizing oxygen *** D-SFN electrode demonstrates outstanding activity and durability,achieving a peak power density of 596 mW cm^(−2)in fuel cell mode and a current density of−1.19 A cm^(−2)in electrolysis mode at 1.3 V,650℃,with excellent cycling *** approach holds the potential for advancing robust and efficient air electrodes in RePCCs for renewable energy storage.

关键词： Reversible protonic ceramic cells Air electrode Ruddlesden-Popper perovskite Hydration Oxygen reduction reaction

Facile synthesis of W-Mo bimetallic oxides with high adsorption properties from secondary resources

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Tungsten 2024年第4期6卷 806-820页

作者： Jia-Li Cheng Yang-Si liu xiao-Li xi Zuo-Ren Nie Collaborative Innovation Center of Capital Resource-Recycling Material Technology College of Materials Science and Engineering Beijing University of Technology National Engineering Laboratory for Industrial Big-Data Application Technology Beijing University of Technology Key Laboratory of Advanced Functional Materials Ministry of Education Beijing University of Technology

A simple solvothermal method was used to obtain W-Mo bimetallic oxides from W-Mo alloy scrap,and pure metal powders were also used as the raw materials to simulate *** products had a sea urchin-like structure with abundant oxygen vacancies and the products prepared at low temperatures forms a sosoloid resembling orthorhombic *** WMo bimetallic oxide prepared at the reaction temperature of 120 ℃ exhibited excellent selective adsorption performance for methylene blue（MB）,which the adsorption rate of MB reached 99% in 12 min and the adsorption rate reached 90% after6 adsorption *** the W-Mo molar ratio is 1:3,the maximum adsorption capacity of sample for MB can reach1148 mg·*** adsorption process followed the Langmuir and pseudo-second-order models,which is surface-controlled monolayer *** experimental results show the feasibility of preparing W-Mo bimetal oxide products from pure materials and *** process is simple and effective,which offered a potential approach for secondary resource recycling and reusing.

关键词： Oxygen vacancy Selective adsorption Solvothermal W–Mo alloy scrap W–Mo bimetallic oxide

Low-energy-consumption temperature swing system for CO_(2) capture by combining passive radiative cooling and solar heating

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Green Energy & Environment 2024年第3期9卷 507-515页

作者： Ying-xi Dang Peng Tan Bin Hu Chen Gu xiao-Qin liu Lin-Bing Sun State Key Laboratory of Materials-Oriented Chemical Engineering Jiangsu National Synergetic Innovation Center for Advanced Materials(SICAM)College of Chemical EngineeringNanjing Tech UniversityNanjing211816China

Temperature-swing adsorption(TSA)is an effective technique for CO_(2) capture,but the temperature swing procedure is ***,we report a low-energy-consumption system by combining passive radiative cooling and solar heating for the uptake of CO_(2) on commercial activated carbons(CACs).During adsorption,the adsorbents are coated with a layer of hierarchically porous poly(vinylidene fluoride-co-hexafluoropropene)[P(VdF-HFP)HP],which cools the adsorbents to a low temperature under sunlight through radiative *** desorption,CACs with broad absorption of the solar spectrum are exposed to light irradiation for *** heating and cooling processes are completely driven by solar *** tests under mimicked sunlight using the CACs show that the performance of this system is comparable to that of the traditional ***,under real sunlight irradiation,the adsorption capacity of the CACs can be well maintained after multiple *** present work may inspire the development of new temperature swing procedures with little energy consumption.

关键词： CO_(2)capture Solar heating Passive radiative cooling Temperature swing adsorption

A post-modification strategy to precisely construct dual-atom sites for oxygen reduction electrocatalysis

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Journal of Energy Chemistry 2024年第3期90卷 511-517,I0012页

作者： Juan Wang xinyan liu Chang-xin Zhao Yun-Wei Song Jia-Ning liu xi-Yao Li Chen-xi Bi xin Wan Jianglan Shui Hong-Jie Peng Bo-Quan Li Jia-Qi Huang School of Materials Science and Engineering Beijing Institute of TechnologyBeijing 100081China Advanced Research Institute of Multidisciplinary Science Beijing Institute of TechnologyBeijing 100081China Beijing Key Laboratory of Green Chemical Reaction Engineering and Technology Department of Chemical EngineeringTsinghua UniversityBeijing 100084China Institute of Fundamental and Frontier Sciences University of Electronic Science and Technology of ChinaChengdu 611731SichuanChina School of Materials Science and Engineering Beihang UniversityBeijing 100191China

Dual-atom catalysts(DACs) afford promising potential for oxygen reduction electrocatalysis due to their high atomic efficiency and high intrinsic ***,precise construction of dual-atom sites remains a *** this work,a post-modification strategy is proposed to precisely fabricate DACs for oxygen reduction ***,a secondary metal precursor is introduced to the primary single-atom sites to introduce direct metal-metal interaction,which ensures the formation of desired atom pair structure during the subsequent pyrolysis process and allows for successful construction of *** as-prepared FeCo-NC DAC exhibits superior oxygen reduction electrocatalytic activity with a half-wave potential of 0,91 V *** hydrogen ***-air batteries equipped with the FeCo-NC DAC demonstrate higher peak power density than those with the Pt/C *** importantly,this post-modification strategy is demonstrated universal to achieve a variety of dual-atom *** work presents an effective synthesis methodology for precise construction of catalytic materials and propels their applications in energy-related devices.

关键词： Dual-atom catalysts Electrocatalysis Oxygen reduction reaction Post-modification Zinc–air batteries

Single-Cell Mapping of Brain Myeloid Cell Subsets Reveals Key Transcriptomic Changes Favoring Neuroplasticity after Ischemic Stroke

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Neuroscience Bulletin 2024年第1期40卷 65-78页

作者： Fangxi liu xi Cheng Chuansheng Zhao xiaoqian Zhang Chang liu Shanshan Zhong Zhouyang liu xinyu Lin Wei Qiu xiuchun Zhang Department of Neurology.The First Affiliated Hospital of China Medical University Shenyang 110001China Department of Neurology The Third Affiliated Hospital of Sun Yat-sen UniversityGuangzhou 510630China Stroke Center The First Affiliated Hospital of China Medical UniversityShenyang 110001China

Interactions between brain-resident and periph-eral infiltrated immune cells are thought to contribute to neuroplasticity after cerebral ***,con-ventional bulk sequencing makes it challenging to depict this complex immune *** single-cell RNA sequencing,we mapped compositional and transcriptional features of peri-infarct immune *** were the predominant cell type in the peri-infarct region,displaying a more diverse activation pattern than the typical pro-and anti-inflammatory state,with axon tract-associated micro-glia(ATMs)being associated with neuronal *** inference suggested that infiltrated monocyte-derived macrophages(MDMs)exhibited a gradual fate trajectory transition to activated ***-cellular crosstalk between MDMs and microglia orchestrated anti-inflammatory and repair-promoting microglia phenotypes and promoted post-stroke neurogenesis,with SOX2 and related Akt/CREB signaling as the underlying *** description of the brain's immune landscape and its relationship with neurogenesis provides new insight into promoting neural repair by regulating neuroinflammatory responses.

关键词： Ischemic stroke Monocyte-derived macrophage Microglia Neurogenesis Single-cell sequencing

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柴油机排气微粒旋流净化技术的初步研究

内燃机学报 2000年第2期18卷 141-144页

作者：宁智刘双喜资新运何永生史绍熙北方交通大学机电学院北京100044 天津大学清华大学

根据柴油机排气微粒净化的要求 ,研制了一台柴油机排气微粒轴流直流式旋流净化器 ,并对旋流净化器的净化特性进行了初步的理论分析和试验研究。研究结果表明 ,排气微粒旋流净化技术对净化柴油机排气微粒是有效的 ;柴油机运行工况及旋流... 详细信息

根据柴油机排气微粒净化的要求 ,研制了一台柴油机排气微粒轴流直流式旋流净化器 ,并对旋流净化器的净化特性进行了初步的理论分析和试验研究。研究结果表明 ,排气微粒旋流净化技术对净化柴油机排气微粒是有效的 ;柴油机运行工况及旋流子结构参数等对排气微粒净化效率有一定影响 ;排气微粒的凝聚是微粒旋流分离净化的前提。通过对净化器结构的优化设计以及采取必要的微粒凝聚措施 ,相信会显著地提高柴油机排气微粒旋流净化器的净化效率。

关键词：柴油机微粒旋流分离器旋流净化排气净化器