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Studies on Fragmentation of Peptide by Nano-electrospray Ionization Fourier Transform Ion Cyclotron Resonance Multi-stage Tandem Mass Spectrometry

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Chemical Research in Chinese Universities 2011年第5期27卷 773-776页

作者： SHI ying liu Ning SONG Feng-rui liu Zhi-qiang liu shu-ying Changchun Centre of Mass Spectrometry Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 P. R. China Central Laboratory the Second Hospital of Jilin University Changchun 130041 P. R. China Graduate School of the Chinese Academy of Sciences Beijing 100049 P. R. China

The sequence analysis of peptides was performed by nano-electrospray ionization Fourier transform ion cyclotron resonance tandem mass spectrometry（Nano-ESI-FT-ICR-MSn） and several peptides were chosen as examples. With the aid of the collision induced dissociation（CID）, FT-ICR provides not only precise mass/charge ratio, but also structure information of the selected peptides. The fragment ions were identified according to the observed molecular weights and peptide sequence was determined successfully. So Nano-ESI-FT-ICR-MSn is a useful tool for identification of the amino acid sequence of peptides with high confidence. Besides, a pathway for the dehydration of y ions without amino acids containing carboxylic acid under sustained off-resonance irradiation collision-induced dissociation（SORI-CID） condition was proposed.

关键词： Nano-electrospray ionization Fourier transform ion cyclotron resonance tandem mass spectrometry(Nano-ESI-FT-ICR-MS) Collision induced dissociation(CID) Peptide sequence

Qualitative and Semi-quantitative Analysis of Quaternary Alkaloids in Coptis-scute Herb Couple by Electrospray Mass Spectrometry

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Chemical Research in Chinese Universities 2008年第2期24卷 162-166页

作者： LI Wei SONG Feng-rui liu Zhi-qiang liu shu-ying Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun Center of Mass Spectrometry Changchun 130022 P. R. China Graduate School of the Chinese Academy of Sciences Beijing 100039 R R. China

A practical solution of qualitatively analyzing quaternary alkaloids in coptis-scute herb couple by electrospray ionization mass spectrometry（ES1-MS） was developed. Without the complicated pretreatment of sample, thc active ingredients including berberine, palmatine, coptisine, jatrorrhizine, epiberberine, and columbamine were identified and some relative content changing rules of alkaloids in coptis-scute couple were summarized in this article. The overall profiles of the complex extracts were obtained. After adding an internal standard（rutaecarpine）, semi-quantitative analysis was performed and the result indicates that the actual content of alkaloids was decreased by increasing the amount of scute. Based on the data obtained by high-performance capillary electrophoresis（HPCE）, the feasibility of semi-quantitative analysis by ES1-MS was further proved.

关键词： ESI-MS Coptis Scute Quaternary alkaloids

Intestinal Transport and Biotransformation of Resibufogenin and Cinobufagin in Chan Su via HPLC/APCI-MS^n

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Chemical Research in Chinese Universities 2011年第3期27卷 380-384页

作者： HAN Tian-jiao WANG Qing SONG Feng-rui liu Zhong-ying liu Zhi-qiang liu shu-ying Changchun Institute of Applied Chemistry Chinese Academy of SciencesChangchun Center of Mass SpectrometryChangchun 130022P.R.China Department of Endocrinology of China-Japan Union Hospital of Jilin University Changchun 130033P.R.China School of Pharmacy Jilin UniversityChangchun 130033P.R.China Graduate School of the Chinese Academy of Sciences Beijing 100049P.R.China

In vitro models of human colon carcinoma cell line(Caco-2 cell monolayer) and human intestinal bacteria were used to investigate the intestinal transport and biotransformation of resibufogenin and cinobufagin in Chan Su by HPLC/APCI-MSn. The experimental results of Caco-2 cell monolayer demonstrate that the apparent permeability coefficients(Papp) of resibufogenin and cinobufagin are higher than 10–6 cm/s, which indicates that both resibufogenin and cinobufagin have a good absorption in the small intestine. And the biotransformation result of human intestinal bacteria shows that resibufogenin has been transformed to 3-epiresibufogenin and cinobufagin has been transformed to 3-epicinobufagin, deacetylcinobufagin and 3-epideacetycinobufagin, respectively.

关键词： Chan Su Resibufogenin Cinobufagin Caco-2 cell monolayer Human intestinal bacteria

Studies on the Components of Crude and Processed Fructus Corni by ESI-MS^n

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Chemical Research in Chinese Universities 2008年第3期24卷 270-274页

作者： ZHOU Li-li WU Guo-guang liu Zhi-qiang liu shu-ying China University of Mining and Technology Xuzhou 221008 P R. China Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun Center of Mass Spectrometry Changchun 130022 P R. China

The components of crude and processed Fructus Corni were investigated by means of electrospray ionization-tandem mass spectrometry（ESI-MSn） technique in the negative ion mode. Compared with those of crude Fructus Corni, the chemical components of the processed Fructus Corni were changed both in quality and in quantity. From the ESI-MS spectra of the crude and processed Fructus Corni, six peaks were selected to establish the characte-ristic ESI-MS peaks. Several factors in the processing procedure were examined. The experimental results demonstrate that the chemical reactions that occurred in the processing procedure can be used for the elucidation of the processed mechanism of Fructus Corni, which is regularly affected by the processing conditions. The present article provides both the chemistry evidence for the understanding of the processing procedure of Fructus Corni and the specific methodology for the research of the processing procedure and quality identification of traditional Chinese medicine.

关键词： Fructus Corni ESI-MSn Processed

Differential Proteomic Analysis of Neonatal Cardiomyocytes in Response to β-Adrenergic Receptor Stimulation

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Chemical Research in Chinese Universities 2006年第3期22卷 319-323页

作者： LI Zi-jian liu Ning liu Zhi-qiang liu shu-ying HAN Qi-de ZHANG You-yi Institute of Vascular Medicine Peking University Third Hospital and Key Laboratory ofMolecular Cardiovascular Sciences Ministry of Education Beijing 100083 P. R. China Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 P. R. China

β-Adrenoceptors（β-ARs） play a critical role in regulating cardiac functions under both physiological and pathological conditions. To further explore the mechanisms through which β-ARs perform its actions, proteomic approaches were adopted to study the global protein patterns in cultured neonatal rat cardiomyocytes exposed to iseproterenol （ISO）. A modified method, ＂Mirror Images in One Gel＂, was used to improve the reproducibility and resolution power of two-dimensional electrophoresis. A 2-DE map with a good reproducibility was obtained in which 1281 ± 70 spots were detected and about 1191± 54 spots were matched, with an average matching rate of 92. 9%. Nine proteins with significant changes were identified by using peptide mass fingerprinting（PMF） data obtained vht MALDI-MS.

关键词： Proteome Two-dimensional electrophoresis(2-DE) MALDI-MS β-Adrenoceptor Cardiomyocyte

Analysis of Norditerpenoid Alkaloids Extracted from Aconitum sinomantanum Nakai by Electrospray Ionization Tandem Mass Spectrometry

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Chemical Research in Chinese Universities 2006年第3期22卷 343-346页

作者： XU Qing-xuan YUE Hao liu Zhi-qiang WANG Yong YAN Cun-yu liu shu-ying Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 P. R. China

Electrospray ionization mass spectrometry（ESI-MS） was applied simultaneously in determining norditerpenoid alkaloids from the roots of Aconitum sinomantanum Nakai （RAS） based on molecular mass information. The tandem mass spectra（ ESI-MS^n） provided the alkaloidal structural information, through which the existence of these alkaloids was further confirmed. Accordingly, six known norditerpenoid alkaloids were simultaneously determined on the basis of their ESI-MS^n spectra. Furthermore, based on the diagnostic fragmentation pathways of alkaloidal MS^n, a rapid method for direct detection and characterization of alkaloids from an ethanolic extract of RAS was described.

关键词： Electrospray ionization tandem mass spectrometry( ESI-MS^n) Norditerpenoid alkaloid Aconitum sinomantanum Nakai

Rapid Profiling of Alkaloids in Several Medicinal Herbs by Matrix-assisted Laser Desorption/Ionization Mass Spectrometry

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Chemical Research in Chinese Universities 2010年第1期26卷 11-16页

作者： LU Lin YUE Hao SONG Feng-rui TSAO Rong liu Zhi-qiang liu shu-ying Changchun Center of Mass Spectrometry Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 P. R. China Guelph Food Research Centre Agriculture and Agri-Food Canada 93 Stone Road West Guelph Ontario N1G5C9 Canada Graduate School Chinese Academy of Sciences Beijing 100049 P. R. China

A simple method was developed for rapid and direct profiling of alkaloids in medical herbs via matrix-assisted laser desorption/ionization time-of-flight mass spectrometry（MALDI-TOF MS）. The dry herbs were first ground to powder and passed through a stainless steel sieve, mixed with the matrix solution to form a homogeneous suspension, which was then directly applied to MALDI analysis. Several matrices were investigated and 2,5- dihydroxybenzoic acid（DHB） was chosen as the optimized one, and the particle with small size was found to favor the analysis. Using this method, the profiles of alkaloids in several medical herbs were readily obtained, and the toxicities of crude and processed Radix Aconiti Lateralis Preparata were compared via the relative intensities of the peaks of the corresponding toxic components shown in their MALDI spectra. This method therefore provides a rapid and reliable protocol for obtaining profiles of alkaloids in medical herbs by using MALDI-TOF MS.

关键词： Matrix-assisted laser desorption/ionization Alkaloid Medical herb

BET Surface Area and Porous Structure of Fullerenes and Fullerene Soots

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Chemical Research in Chinese Universities 1999年第1期15卷 92-95页

作者： CHEN Jian-xin SHENG Guo-ying liu shu-ying liu Zi-yang liu Zhi-qiang FU Jia-mo ZHANG Xiao-mei Changchun Institute of Applied Chemistry a Chinese Academy of SciencesChangchun130022 State Key Laboratory of Organic Geochemistry Guangzhou Institute of Geochemistry Chinese Academy of ScienoesGuangzhou510640 Institute of Animal Saience Guangdong Acadeny of Agricultural Science510640 Environmental Monitoring Centeral Station off Jilin Province Changchun130011

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ＩｎｔｒｏｄｕｃｔｉｏｎＡｆａｓｃｉｎａｔｉｎｇｓｏｌｉｄｂｌａｃｋｃｏｎｄｅｎｓａｔｅ（ｈｅｒｅｃａｌｅｄｆｕｌｅｒｅｎｅｓｏｏｔ）ｉｓｇｅｎｅｒａｔｅｄｉｎｔｈｅｇａｓｐｈａｓｅａｎｄｄｅｐｏｓｉｔｅｄｄｕｒｉｎｇｔｈｅｄｉｓｃｈａｒｇｅｉｎａＫ...

关键词： Fullerenes Fullerene soots Surface area Pore size distribution Adsorbent

Candidate genes involved in the biosynthesis of lignan in Schisandra chinensis fruit based on transcriptome and metabolomes analysis

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Chinese Journal of Natural Medicines 2020年第9期18卷 684-695页

作者： CHEN Chun-Yu liu shu-ying YAN Yan YIN Le DI Peng liu Hui-Min liu Hong-Zhang College of life Science Jilin Agricultural UniversityChangchun 130118China College of Chinese Medicinal Materials Jilin Agricultural UniversityChangchun 130118China

Schisandra chinensis Turcz.(Baill.) is a plant species with fruits that have been well known in Far Eastern medicine for a long time. It has traditionally been used as a stimulating and fortifying agent in cases of physical exhaustion and to inhibit *** major bioactive compounds found in S. chinensis are lignans with a dibenzocyclooctadiene skeleton, but little is known about their biosynthesis in plants. S. chinensis is the ideal medicinal plant for studying the biosynthesis of lignans, especially the dibenzocyclooctadiene skeleton. Genomic information for this important herbal plant is unavailable. To better understand the lignan biosynthesis pathway, we generated transcriptome sequences from the fruit during ripening and performed de novo sequence assembly, yielding136 843 unique transcripts with N50 of 1778 bp. Putative functions could be assigned to 41 824 transcripts(51.57%) based on BLAST searches against annotation databases including GO(Gene ontology) and KEGG(Kyoto Encyclopedia of Genes and Genomes). Furthermore, 22 candidate cytochrome P450 genes and 15 candidate dirigent proteins genes that were most likely involved in the lignan biosynthesis pathway were discovered based on transcriptome sequencing of S. chinensis. The genomic data obtained from S. chinensis, especially the identification of putative genes involved in the lignan biosynthesis pathway, will facilitate our understanding of lignan biosynthesis at the molecular level. The lignan metabolite profiles were analyzed by metabolomes, the accumulation patterns of 30 metabolites involved in the lignan pathway were studied. Co-expression network of lignan contents and transcriptional changes showed355 strong correlations(correlation coefficient, R^2 > 0.9) between 21 compounds and 153 transcripts. Furthermore, the comprehensive analysis and characterization of the genes involved in lignan pathways and the metabolite profiles of lignans are expected to provide better insight regarding the dive

关键词： Schisandra chinensis Transcriptome Metabolome Biosynthetic pathways Lignan