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Development of a Novel PmpD-N ELISA for Chlamydia psittaci Infection

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Biomedical and Environmental Sciences 2016年第5期29卷 315-322页

作者： liu shan shan CHU Jun ZHANG Qiang SUN Wei ZHANG Tian Yuan HE Cheng Key Lab of Animal Epidemiology and Zoonosis of Ministry of Agriculture College of Veterinary MedicineChina Agricultural UniversityBeijing 100193China Tongren Polytechnic College Tongren 554300GuizhouChina National and Local Engineering Research Centre for Separation and Purification Ethnic Chinese Veterinary Herbs Tongren 554300GuizhouChina

Objective Chlamydia psittaci is an avian respiratory pathogen and zoonotic agent. The wide prevalence of C. psittaci poses a threat to the poultry industry and its employees. However, few commercial kits are available for detecting avian antibodies excluding the in-house ELISA kit. In this study, we developed a novel ELISA kit for detecting antibodies against C. psittaci based on the N-terminal fragment of polymorphic outer membrane protein D （PmpD-N） as the coating antigen. Methods The antigen concentrations, primary antibody, and cut-off value were determined and optimized. The ELISA, designated PmpD-N ELISA, was assessed for sensitivity, specificity, and concordance using sera samples from 48 experimentally infected and 168 uninfected SPF chickens. Results The sensitivity and specificity of PmpD-N ELISA were 97.9%, 100%, respectively, while the concordance was 98.1% as compared to that of MOMP-ELISA. No cross-reaction with positive sera for other avian pathogens was found. Using PmpD-N ELISA, 799/836 clinical samples were positive, including 93.0% and 98.1% positivity in layers and broilers, respectively. Conclusion These data indicate that indirect ELISA with PmpD-N as the antigen candidate is a promising approach for the surveillance of C. psittaci infection.

关键词： Chlamydia psittaci Indirect ELISA N-terminal fragment of polymorphic membrane protein D Seroprevalence

Induction of actin disruption and downregulation of P-glycoprotein expression by solamargine in multidrug-resistant K562/A02 cells

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Chinese Medical Journal 2011年第13期124卷 2038-2044页

作者： LI Xia ZHAO Ying JI Mei liu shan-shan CUI Min LOU Hong-xiang School of Pharmaceutical Sciences Shandong University JinanShandong 250012 China School of Ocean Shandong University Weihai Shandong 264209China

Background Solamargine （SM）, a steroidal glycoalkaloid isolated from the Chinese herb Solanum incanum, has been shown to inhibit the growth of some cancer cell lines and induce significant apoptosis. However, the effects of SM on multidrug-resistant （MDR） cells and the molecular mechanisms involved are poorly understood. The purpose of this study was to evaluate the anti-MDR effects of SM and the associated mechanisms in MDR K562/A02 cells. Methods The cytotoxicity of SM was measured by 3-（4,5-dimethylthiazol）-2,5-diphenyltetrazolium bromide （MTT） assay. The 14＇,6-diamidino-2-phenylindole （DAPi） nuclear staining and flow cytometry were used to detect SM-induced apoptosis. The mRNA expression of P-glycoprotein （P-gp） was investigated by real-time PCR （RT-PCR）. Western blotting was used to determine the expression of Bcl-2, Bax, and actin. The changes in the morphology of actin were examined with immunofluorescence staining. Results MTT results showed that SM effectively killed the MDR sublines K562/A02, KB/VCR, and H460/paclitaxel （Taxol）, and their parental cell lines K562, KB, and H460 to an equivalent or more sensitive degree. Based on the results by flow cytometry and immunostaining, the pro-apoptotic effects of SM were observed in MDR K562/A02 cells. Furthermore, the RT-PCR results showed that SM induced the downregulation of MDR1 mRNA. In addition, the expression of P-gp and actin was decreased in the SM-treated cells, as measured by western blotting and immunostaining. Conclusions These results demonstrate that SM effectively triggers apoptosis in MDR tumor cells, which is associated with actin disruption and downregulation of MDR1 expression. This compound may merit further investigation as a Potential therapeutic aaent that bvDasses the MDR mechanism for the treatment of MDR tumors.

关键词： solamargine multidrug-resistant apoptosis P-glycoprotein

黄连阿胶汤加减联合自制安神睡眠贴治疗失眠患者效果及对睡眠质量指数的影响

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现代生物医学进展 2024年第17期24卷 3237-3240+3364页

作者：蔡巍巍杜莹莹刘珊珊刘轶菲夏伟南京医科大学附属江宁医院中医科

目的：研究使用中药方剂黄连阿胶汤联合自制安神睡眠贴治疗失眠患者临床疗效及对其睡眠质量指数的影响。方法：选取2021年1月到2023年12月在我院接受治疗103失眠患者，随机数表法分为对照组（51例）和研究组（52例），对照组患者予以常... 详细信息

目的：研究使用中药方剂黄连阿胶汤联合自制安神睡眠贴治疗失眠患者临床疗效及对其睡眠质量指数的影响。方法：选取2021年1月到2023年12月在我院接受治疗103失眠患者，随机数表法分为对照组（51例）和研究组（52例），对照组患者予以常规西药治疗，研究组患者在常规西药治疗的基础上加用黄连阿胶汤联合自制安神睡眠贴进行治疗，比较两组患者临床治疗疗效、治疗前后睡眠质量指数（PSQI）和生活质量（QOL）总分，以及治疗后全睡眠时间和睡眠觉醒次数。结果：研究组治疗后临床疗效有效率高于对照组（P<0.05）。两组患者治疗后PSQI总分均显著下降和QOL总分均上升，并且研究组与对照组比较有相同趋势（P<0.05）。治疗后，研究组患者全睡眠时间显著高于对照组患者（P<0.05），而睡眠觉醒次数显著低于对照组患者（P<0.05）。结论：黄连阿胶汤联合自制安神睡眠贴对失眠患者具有很好的临床治疗疗效，并且有助于降低其睡眠指数和提高生活质量，值得进行临床推广。

关键词：黄连阿胶汤安神睡眠贴失眠睡眠质量指数生活质量

老年高血压伴衰弱患者认知功能障碍的危险因素及与不良预后的关系

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心血管康复医学杂志 2024年第4期33卷 391-394页

作者：赵璨刘姗姗李净兵王少颖王莉迪刘俊伏河北省第八人民医院心内科河北石家庄050000 河北省第八人民医院老年科河北石家庄050000 河北省第八人民医院神经内科河北石家庄050000

目的:探讨老年高血压伴衰弱患者认知功能障碍的危险因素及与不良预后的关系。方法:选择2019年7月至2021年3月河北省第八人民医院收治的老年高血压伴衰弱患者150例,根据是否存在认知功能障碍分为认知正常组(92例)和认知障碍组(58例),收... 详细信息

目的:探讨老年高血压伴衰弱患者认知功能障碍的危险因素及与不良预后的关系。方法:选择2019年7月至2021年3月河北省第八人民医院收治的老年高血压伴衰弱患者150例,根据是否存在认知功能障碍分为认知正常组(92例)和认知障碍组(58例),收集两组患者一般资料、预后情况。采用多因素Logistic回归分析老年高血压伴衰弱患者认知功能障碍的危险因素,采用Spearman相关性分析法分析认知功能障碍程度与预后的相关性。结果:认知障碍组患者年龄较认知正常组大,存在糖尿病、冠心病、脑卒中、低钠血症、多重用药、营养不良的比例显著高于认知正常组(P<0.05或<0.01)。多因素Logistic回归分析显示,年龄≥75岁、脑卒中、多重用药、营养不良是老年高血压伴衰弱患者发生认知功能障碍的独立危险因素(OR=2.804~6.434,P<0.05或<0.01)。认知障碍组预后不良事件发生率显著高于认知正常组(51.72%比11.96%,P<0.001)。Spearman相关性分析显示,老年高血压伴衰弱患者认知功能障碍与不良预后呈显著正相关(r=0.435,P<0.001)。结论:年龄、脑卒中、多重用药、营养不良是老年高血压伴衰弱患者认知功能障碍的独立危险因素,认知功能障碍与此类患者不良预后密切相关。

关键词：高血压认知障碍危险因素预后

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医疗器械包装运输测试标准应用对比分析

标准科学 2024年第2期 70-74,80页

作者：刘姗姗冯智劼李学洋张闯李涛天津海关工业产品安全技术中心天津海关化矿金属材料检测中心

本文以医疗器械包装运输性能测试应用为主旨,系统分析国内外医疗器械包装运输标准异同。文章比对分析国际运输安全协会、美国标准、我国国家标准以及医药行业标准的主要适用范围、测试项目差异,并以质量为50kg的医疗器械包装件为例,分... 详细信息

本文以医疗器械包装运输性能测试应用为主旨,系统分析国内外医疗器械包装运输标准异同。文章比对分析国际运输安全协会、美国标准、我国国家标准以及医药行业标准的主要适用范围、测试项目差异,并以质量为50kg的医疗器械包装件为例,分析运输测试温湿度条件、低气压、振动、冲击、堆码试验参数等效性与差异性。分别对有源、无菌和涉及中美双报的医疗器械包装运输测试提出应重点关注的项目,为开展医疗器械全生命周期监管提供技术支持。

关键词：医疗器械包装运输稳定性标准对比分析

Facet effects on bimetallic ZnSn hydroxide microcrystals for selective electrochemical CO2 reduction

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Green Energy & Environment 2024年第8期9卷 1314-1320页

作者： liu Han Cheng-wei Wang shan-shan Luo Ying-tang Zhou Bing Li Ming liu School of Chemistry and Chemical Engineering Harbin Institute of Technology National Engineering Research Center for Marine Aquaculture Marine Science and Technology CollegeZhejiang Ocean University

Employing crystal facets to regulate the catalytic properties in electrocatalytic carbon dioxide reduction reaction（eCO2RR） has been well demonstrated on electrocatalysts containing single metals but rarely explored for bimetallic systems. Here, we synthesize Zn Sn（OH）6（ZSO）microcrystals（MCs） with distinct facets and investigate the facet effects in eCO2RR. Electrochemical studies and in situ Fourier Transform Infrared Spectroscopy（in situ-FTIR） reveal that ZSO MCs produce mainly C1 products of HCOOH and CO. The {111} facet of the ZSO MCS exhibits higher selectivity and faradaic efficiency（FE） than that of the {100} facet over a wide range of potentials（-0.9 V ～-1.3 V versus RHE）. Density Functional Theory（DFT） calculations elucidate that the {111} facet is favorable to the adsorption/activation of CO2molecules,the formation of intermediate in the rate-determining step, and the desorption of C1 products of CO and HCOOH molecules.

关键词： ZnSn(OH) 6 Crystal facets Electrochemical carbon dioxide reduction C1 product Density functional theory

An artificial intelligence diabetes management architecture based on 5G

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Digital Communications and Networks 2024年第1期10卷 75-82页

作者： Ruochen Huang Wei Feng shan Lu Tao shan Changwei Zhang Yun liu Department of Geriatrics The First Affiliated Hospital of Nanjing Medical UniversityNanjing210096China Department of Information The First Afliated Hospital of Nanjing Medical UniversityNanjing210096China Department of Medical Informatics School of Biomedical Engineering and InformaticsNanjing Medical UniversityNanjing211166China Department of Outpatient The First Affiliated Hospital of Nanjing Medical UniversityNanjing210096China Jiangsu Key Laboratory of Wireless Communications Nanjing University of Posts and TelecommunicationsNanjing210003China

Along with the development of 5G network and Internet of Things technologies,there has been an explosion in personalized healthcare *** the 5G and Artificial Intelligence(Al)is introduced into diabetes management architecture,it can increase the efficiency of existing systems and complications of diabetes can be handled more effectively by taking advantage of *** this article,we propose a 5G-based Artificial Intelligence Diabetes Management architecture(AIDM),which can help physicians and patients to manage both acute complications and chronic *** AIDM contains five layers:the sensing layer,the transmission layer,the storage layer,the computing layer,and the application *** build a test bed for the transmission and application ***,we apply a delay-aware RA optimization based on a double-queue model to improve access efficiency in smart hospital wards in the transmission *** application layer,we build a prediction model using a deep forest *** on real-world data show that our AIDM can enhance the efficiency of diabetes management and improve the screening rate of diabetes as well.

关键词： Diabetes 5G Artificial intelligence Deep forest Smart hospital ward

GB9706.1-2020医用电气设备基本安全和基本性能的通用要求检测仪器设备分析

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中国医疗器械信息 2024年第3期30卷 22-24,168页

作者：李学洋张婧张寅豹刘姗姗李晶于智睿天津海关工业产品安全技术中心天津300457

针对GB9706.1-2020医用电气设备基本安全和基本性能的通用要求,按照标准条款按顺序归纳出具体测试项目,确定检测参数所需仪器设备,对照标准要求细化仪器设备技术参数及环境实施要求。有效解决将抽象的标准条款与具体检测测试及所用仪器... 详细信息

针对GB9706.1-2020医用电气设备基本安全和基本性能的通用要求,按照标准条款按顺序归纳出具体测试项目,确定检测参数所需仪器设备,对照标准要求细化仪器设备技术参数及环境实施要求。有效解决将抽象的标准条款与具体检测测试及所用仪器设备相互结合转化的问题,为开展GB9706.1检测提供技术支持。

关键词：医用电气设备安全通用要求仪器设备

不同剂型棕榈酸帕利哌酮治疗精神分裂症有效性及安全性分析

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临床药物治疗杂志 2024年第2期22卷 26-30页

作者：贾菲臧彦楠鲍爽牛梦溪刘珊珊庄红艳郑楠首都医科大学附属北京安定医院国家精神心理疾病临床医学研究中心精神疾病诊断与治疗北京市重点实验室北京100088 人脑保护高精尖创新中心首都医科大学北京100069

目的比较1个月注射1次的棕榈酸帕利哌酮注射液(PP1M)与3个月注射1次的棕榈酸帕利哌酮注射液(PP3M)在有效性和安全性方面的差异。方法检索中国知网、万方数据知识服务平台、PubMed、Embase、the cochrane Library等数据库中关于PP1M与P... 详细信息

目的比较1个月注射1次的棕榈酸帕利哌酮注射液(PP1M)与3个月注射1次的棕榈酸帕利哌酮注射液(PP3M)在有效性和安全性方面的差异。方法检索中国知网、万方数据知识服务平台、PubMed、Embase、the cochrane Library等数据库中关于PP1M与PP3M有效性及安全性比较研究的文献,检索时间为数据库建库至2023年11月。结果共纳入10篇文献,研究对象均为成人精神分裂症患者,研究周期7~24个月。结果显示,PP1M和PP3M对于精神分裂症患者的症状改善程度相似,PP3M比PP1M更能降低因精神疾病住院率和疾病复发风险,使用PP3M发生锥体外系综合征和体质量增加的风险低于PP1M,其他安全性指标在二者之间没有明显区别。结论 PP1M和PP3M的疗效与安全性相当,PP3M用药依从性更好。

关键词：棕榈酸帕利哌酮长效注射液有效性安全性精神分裂症