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Effects of Protein on the Detection of Chlorobenzenes in Fish and Their Elimination by Modified QuEChERS Method

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Chemical Research in Chinese Universities 2013年第6期29卷 1063-1067页

作者： DENG Qiao-qiao li Gui-zhi liu pei-pei ZHANG Xing-mei liu yong-ming College of Chemistry and Chemical Engineering Yantai University Yantai 264005 P.R.China

Molecular fluorescence spectrometry, resonance Rayleigh scattering and gas chromatography-mass spectroscopy（GC-MS） were used to study the effect of pure fish serum albumin（FSA） as the model protein on the extraction of three chlorobenzenes（CBs： 1,3-DCB, 1,2-DCB and 1,2,4-TCB; DCB=dichlorobenzene; TCB=trichlorobenzene） in fish samples. The results show that there was a strong binding effect between CB and FSA. In an aqueous solution of 90%（volume fraction） acetone, a slow but full protein denaturation might take place, which would cause the unfolding of protein and the releasing of CBs. Based on these results, a QuEChERS（quick, easy, cheap, effective, rugged and safe） method was modified by replacing the traditional acetonitrile with acetone aqueous solution in the present work. This modified QuEChERS method was applied in the determination of CBs in fish samples. The spiked recoveries and the limits of detection were 80.4%--118.3% and 2.4--7.3 ng/g, respectively. This paper proposes a new strategy by slowing down the protein denaturation and releasing bound organic compounds to enhance the extraction efficiency of CBs in fish samples.

关键词： Chlorobenzene Modified QuEChERS(quick,easy,cheap,effective,rugged and safe) method Gas chromatography-mass spectroscopy Fluorescence spectrometry Resonance Rayleigh scattering

Single Ti atoms coupled with Ti-O clusters enable low temperature hydrogen cycling by sodium alanate

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Rare Metals 2024年第6期43卷 2671-2681页

作者： Zhuang-He Ren Xin Zhang wen-xuan Zhang Zhen-Guo Huang li-Mei Yang Ya-Xiong Yang Zheng-Long li Juan li wen-Ping Sun ming-Xia Gao Hong-Ge Pan yong-Feng liu State Key Laboratory of Silicon and Advanced Semiconductor Materials Zhejiang UniversityHangzhou 310027China School of Materials Science and Engineering Zhejiang UniversityHangzhou 310058China School of Civil&Environmental Engineering.University of Technology Sydney Sydney2007 NSWAustralia Institute of Science and Technology for New Energy Xi'an Technological UniversityXi'an 710021China College of Materials Science and Engineering Zhejiang University of TechnologyHangzhou 310014China

Ti-based catalysts are known to improve the hydrogen storage performance of NaAlH4by facilitating the dissociation/recombination of H-H and Al-H *** catalytic activity of metallic Ti species strongly depends on its particle size and *** clusters and even single atoms are therefore highly desirable,but their controllable fabrication has been highly *** rein,we demonstrate a novel facile sonochemical synthesis of a Ti-O clusters featuring single Ti atom catalyst at room *** reducing TiCl_(4)by MgBu_(2)with ultrasound instead of heating as driving force,numerous single Ti atoms coupled with Ti-O clusters with Ti loading on graphene(Ti_(1)/Ti-O@G)up to 22.6 wt%have been successfully *** prepared Ti_(1)/Ti-O@G contributes high reactivity and superior catalytic activity,therefore enabling full dehydrogenation of NaAlH_(4)at 80℃in thermogravimetric mode and re-hydrogenation at 30℃and 10 MPa with 4.9 wt% H_(2).This fact indicates for the first time that single Ti atom catalyst with high loading is highly effective in catalyzing hydrogen cycling of NaAlH4at remarkably reduced temperatures.

关键词： Hydrogen storage Nanocatalysis Transition metals Single Ti atoms NaAlH_(4)

Tailoring electron transfer with Ce integration in ultrathin Co(OH)_(2) nanosheets by fast microwave for oxygen evolution reaction

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Journal of Energy Chemistry 2021年第8期30卷 299-305,I0007页

作者： Ya-Nan Zhou Ruo-Yao Fan Shu-Yue Dou Bin Dong Yu Ma wen-li Yu Meng-xuan li Yu-Lu Zhou Chen-Guang liu yong-ming Chai State Key Laboratory of Heavy Oil Processing China University of Petroleum(East China)Qingdao 266580China College of Science China University of Petroleum(East China)Qingdao 266580China

The intrinsic activity of Co(OH)_(2) for oxygen evolution reaction(OER)may be elaborately improved through the suitable valence *** modification at electronic level is proved to be an efficient strategy owing to the flexible transformation of Ce^(3+)/Ce4+.Herein,Ce0.21@Co(OH)_(2) with the optimized Ce doping have been fabricated to tailor the fast electron transfer for the enhanced activity and stability for ***,the obtained core-shell structure composed of vertical loose Co(OH)_(2) sheets not only exposes a large number of active sites,but also provides channels for Ce ***,the high pressure microwave with instantaneous heating can fast introduce Ce into Co(OH)_(2),obtaining Cex@Co(OH)_(2) with well dispersion and close *** intimated interaction between Ce and Co species may provide the"d-f electronic ladders"for accelerating electron transfer of the catalytic ***,Ce promotes the formation of Co-superoxide intermediate and/or the release of oxygen,which is considered to be the rate-determining step for *** electrochemical measurements confirmed the low overpotential of 300 m V at 10 m A cm^(-2) and great stability of Ce0.21@Co(OH)_(2) for *** work demonstrates a meaningful approach to realize the tuned electronic structure through metal doping.

关键词： Ultrathin Co(OH)_(2) Ce doping Electron regulation High pressure microwave Oxygen evolution reaction

A new cytotoxic caged polyprenylated xanthone from the resin of Garcinia hanburyi

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Chinese Chemical Letters 2008年第10期19卷 1221-1223页

作者： li li Wang Zhan lin li yong Peng Xu Xiao Qiu liu Yue Hu pei yong Kui Jing Hui ming Hua School of Traditional Chinese Materia Medica Shenyang Pharmaceutical University Shenyang 110016 China School of life Science and Biopharmaceutics Shenyang Pharmaceutical University Shenyang 110016 China Department of Medicine Mount Sinai School of Medicine NY 10029-6547 USA

A new caged polyprenylated xanthone, gambogic aldehyde （1）, was isolated from the resin of Garcinia hanburyi. Its structure was elucidated on the basis of spectral data including 1D and 2D NMR data. In addition, the... 详细信息

关键词： Garcinia hanburyi Caged polyprenylated xanthone Antiproliferafive ability

W55a Encodes a Novel Protein Kinase That Is Involved in Multiple Stress Responses

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Journal of Integrative Plant Biology 2009年第1期51卷 58-66页

作者： Zhao-Shi Xu li liu Zhi-yong Ni pei liu ming Chen lian-Cheng li Yao-Feng Chen You-Zhi Ma National Key Facility of Crop Gene Resources and Genetic Improvement (NFCRI) Key Laboratory of Crop Genetics and Breeding Ministry of Agriculture Institute of Crop Science the Chinese Academy of Agricultural Sciences (CAAS) Beijing 100081 China Agronomy College Northwest Sci-Tech University of Agriculture and Forestry Yangling 712100 China

Protein kinases play crucial roles in response to external environment stress signals. A putative protein kinase, W55a, belonging to SNFl-related protein kinase 2 （SnRK2） subfamily, was isolated from a cDNA library of drought-treated wheat seedlings. The entire length of W55a was obtained using rapid amplification of 5＇ cDNA ends （5＇-RACE） and reverse transcription-polymerase chain reaction（RT-PCR）. It contains a 1 029-bp open reading frame （ORF） encoding 342 amino acids. The deduced amino acid sequence of W55a had eleven conserved catalytic subdomains and one Ser/Thr protein kinase active-site that characterize Ser/Thr protein kinases. Phylogenetic analysis showed that W55a was 90.38% homologous with rice SAPK1, a member of the SnRK2 family. Using nullisomic-tetrasomic and ditelocentric lines of Chinese Spring, W55a was located on chromosome 2BS. Expression pattern analysis revealed that W55a was upregulated by drought and salt, exogenous abscisic acid, salicylic acid, ethylene and methyl jasmonate, but was not responsive to cold stress. In addition, W55a transcripts were abundant in leaves, but not in roots or stems, under environmental stresses. Transgenic Arabidopsis plants overexpressing W55a exhibited higher tolerance to drought. Based on these findings, W55a encodes a novel dehydration-responsive protein kinase that is involved in multiple stress signal transductions.

关键词： chromosomal location induction kinetics SnRK2 stress response transgenic Arabidopsis wheat.

The Role of Renewable Protocatechol Acid in Epoxy Coating Modification: Significantly Improved Antibacterial and Adhesive Properties

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Chinese Journal of Polymer Science 2024年第1期42卷 63-72,I0008页

作者： ming-xuan Chen Jin-Yue Dai li-Yue Zhang Shuai-Peng Wang Jing-Kai liu yong-Gang Wu Xin-Wu Ba Xiao-Qing liu School of Chemistry and Materials Science Hebei UniversityBaoding 071000China Ningbo Institute of Materials Technology and Engineering Chinese Academy of SciencesNingbo 315201China Key Laboratory of Marine Materials and Related Technologies Chinese Academy of SciencesNingbo 315201China

It is of great significance to design epoxy coatings with superior antibacterial properties and high adhesive properties, as well as excellent processing, superior durability, and high transparency. However, it is still a challenge because of the common complex design and synthesis. Herein, the bio-based monomer protocatechuic acid(PCA) was used as raw material, the catechol structure with high bonding and antibacterial properties was introduced into the flexible alkane segment of ethylene glycol diglycidyl ether(EGDE) through an efficient, and green method, and it was cured with isophorone diamine(IPDA) to prepare corresponding thermosets. The cured resins exhibited excellent allaround qualities, particularly in bonding and antibacterial. When 30% PCA was added to pure epoxy resin, the adhesion between substrate and coating increased from 4.40 MPa to 13.60 MPa and the antibacterial rate of coating against E. coli and S. aureus could approach 100%. All of this is due to the fact that the catechol structure present in PCA has the ability to interact with various substrates and alter the permeability of bacterial cell membranes. The architecture of this method offers a fresh approach to dealing with the issues of challenging raw material selection and complex synthesis techniques.

关键词： Epoxy coatings Thermosets Catechol structure Antibacterial High adhesion

Stent fracture after transjugular intrahepatic portosystemic shunt placement using the bare metal stent/stent-graft combination technique

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World Journal of Gastrointestinal Surgery 2023年第10期15卷 2133-2141页

作者： Qi-Jia liu Xiao-Feng Cao Yun pei xuan li Guo-Xiang Dong Chang-ming Wang Department of Interventional Radiology and Vascular Surgery Peking University Third HospitalBeijing 100191China Department of Interventional Therapy Yangquan First People’s HospitalYangquan 045000Shanxi ProvinceChina

BACKGROUND A transjugular intrahepatic portosystemic shunt(TIPS)is widely placed to treat portal *** the Viatorr®stent(*** and Associates,Flagstaff,AZ,United States)is not available in all hospitals in China,the bare metal stent(BMS)/stent-graft combination technique is still popular for TIPS *** fracture is a complication after TIPS placement using this technique,with limited available literature focusing on *** To assess the incidence of stent fracture after TIPS placement using the BMS/stent-graft combination technique and to identify the risk factors for stent *** proposed technique modifications to improve the clinical results of TIPS placement with the BMS/stent-graft combination *** We retrospectively analyzed the computed tomography(CT)data of all patients with portal hypertension who underwent the TIPS procedure between June 2011 and December 2021 in a single *** implanted with the BMS/stent graft and had follow-up imaging data available were *** identified patients with stent fracture and analyzed their *** logistic regression was applied to identify the potential predictors of stent *** Of the 68 included patients,stent fracture occurred in seven(10.3%)*** on CT images,the stent fractures were categorized into three *** study consisted of four(57.1%)type I fractures,one(14.3%)type II fracture,one(14.3%)type IIIa fracture,and one(14.3%)type IIIb *** adjusting for covariates,multivariable logistic regression revealed that the risk factors for stent fracture were the implantation of a greater number of stents[adjusted odds ratio(aOR)=22.2,95%confidence interval(CI):1.2-415.4,P=0.038]and a larger proximal sagittal stent bending angle(aOR=1.1,95%CI:1.0-1.3,P=0.020).CONCLUSION Stent fracture occurred in approximately 10%of patients with portal hypertension who underwent TIPS with the BMS/stent-graft combination tec

关键词： Portal hypertension Transjugular intrahepatic portosystemic shunt Stent fracture Bare metal stent/stent-graft combination Risk factor Fracture types

Isosteroidal Alkaloids from Fritillaria verticillata Willd. and Their NMR Spectroscopic Characteristics

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Chinese Journal of Chemistry 2022年第16期40卷 1945-1956页

作者： Nan Ma Ya-Jing Wang Gang Chen Chu-Ren Meng Chun-lin Guo yong-Fu Lu Si-Yang Dai Si-xuan liu yong-Fu li Chang li Yue-Hu pei Department of Medicinal Chemistry and Natural Medicine Chemistry College of PharmacyHarbin Medical UniversityHarbinHeilongjiang150081 China Shenyang Pharmaceutical University ShenyangLiaoning 110016China

Chemical investigation of Fritillaria verticillata *** to the isolation of eight novel isosteroidal alkaloids(1—4,6,9—11)and four known alkaloids(5,7—8,12),including three unprecedented cis-fused D/E(13α,17α)cevanine alkaloids(1—3),one rarely cis-fused E/F(22α)cevanine alkaloid(6),and one uncommon 5β-jervine-type isosteroidal alkaloid featuring a cis-fused A/B ring moiety(11).In order to establish the structures of 1,the calculated NMR with DP4+evaluation was applied from the plausible structure *** characteristic proton signals for distinguishing D/E conjunction of cevanine alkaloids were *** addition,some proton and carbon signalsαto nitrogen in 6 and 7 are unobservable due to the 14N nuclear quadrupolar relaxation,based on the NMR experiments in different solvents,calculated NMR method and X-ray technology,their structures were *** NMR characteristics of cevanine alkaloids to distinguish orientation of long-pair on nitrogen atom withβ-hydroxyl at C-20 were also *** anti-inflammatory effects of compounds 1 and 4 were evaluated in LPS-activated RAW 264.7 *** 4 decreased LPS-induced releases of IL-1βand IL-17αin RAW264.7 cells in a dose-dependent *** mechanistic study revealed that 4 suppressed the phosphorylation of IκBαand p65 subunit to regulate the NF-κB signaling pathway.

关键词： Alkaloids Fritllaria Structure elucidation Spectroscopic characteristics Inflammatory

Molecular detection of the powdery mildew resistance genes in winter wheats DH51302 and Shimai 26

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Journal of Integrative Agriculture 2020年第4期19卷 931-940页

作者： QU Yun-feng WU pei-pei HU Jing-huang CHEN yong-xing SHI Zhan-liang QIU Dan li Ya-hui ZHANG Hong-jun ZHOU Yang YANG li liu Hong-wei ZHU Tong-quan liu Zhi-yong ZHANGYan-ming li Hong-jie Key Laboratory of Molecular Cytogenetic and Genetic Breeding of Heilongjiang Province/College of life Science and Technology Harbin Normal UniversityHarbin 150025P.R.China National Engineering Laboratory for Crop Molecular Breeding/Institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijing 100081P.R.China Institute of Genetics and Developmental Biology Chinese Academy of SciencesBeijing 100101P.R.China Shijiazhuang Academy of Agricultural and Forestry Sciences Shijiazhuang 050041P.R.China Wheat Research Institute Zhumadian Academy of Agricultural SciencesZhumadian 463000P.R.China

Resistance to powdery mildew is an important trait of interest in many wheat breeding programs. The information on genes conferring resistance to powdery mildew in wheat cultivars is useful in parental selection. Winter wheat breeding line DH51302 derived from liangxing 99 and cultivar Shimai 26 derived from Jimai 22 showed identical infection patterns against 13 isolates of Blumeria graminis f. sp. tritici(Bgt) that causes wheat powdery mildew. DH51302 and Shimai 26 were crossed to a powdery mildew susceptible cultivar Zhongzuo 9504 and the F2:3 families were used in molecular localization of the resistance genes. Fourteen polymorphic markers, which were linked to Pm52 from liangxing 99, were used to establish the genetic linkage maps for the resistance genes Pm DH51302 and Pm SM26 in DH51302 and Shimai 26, respectively. These genes were placed in the same genetic interval where Pm52 resides. Analysis of gene-linked molecular markers indicated that Pm DH51302 and Pm SM26 differed from other powdery mildew resistance genes on chromosome arm 2 BL, such as Pm6, Pm33, Pm51, Ml Zec1, Ml AB10, and Pm64. Based on the results of reaction patterns to different Bgt isolates and molecular marker localization, together with the pedigree information, DH51302 and Shimai 26 carried the same gene, Pm52, which confers their resistance to powdery mildew.

关键词： Triticum aestivum Blumeria graminis f.sp.tritici Pm52 molecular mapping