Molecular detection of the powdery mildew resistance genes in winter wheats DH51302 and Shimai 26

作     者：QU Yun-feng WU Pei-pei HU Jing-huang CHEN Yong-xing SHI Zhan-liang QIU Dan LI Ya-hui ZHANG Hong-jun ZHOU Yang YANG Li LIU Hong-wei ZHU Tong-quan LIU Zhi-yong ZHANGYan-ming LI Hong-jie QU Yun-feng;WU Pei-pei;HU Jing-huang;CHEN Yong-xing;SHI Zhan-liang;QIU Dan;LI Ya-hui;ZHANG Hong-jun;ZHOU Yang;YANG Li;LIU Hong-wei;ZHU Tong-quan;LIU Zhi-yong;ZHANGYan-ming;LI Hong-jie

作者机构：Key Laboratory of Molecular Cytogenetic and Genetic Breeding of Heilongjiang Province/College of Life Science and TechnologyHarbin Normal UniversityHarbin 150025P.R.China National Engineering Laboratory for Crop Molecular Breeding/Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081P.R.China Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101P.R.China Shijiazhuang Academy of Agricultural and Forestry SciencesShijiazhuang 050041P.R.China Wheat Research InstituteZhumadian Academy of Agricultural SciencesZhumadian 463000P.R.China 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2020年第19卷第4期

页      面：931-940页

核心收录：

学科分类：09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 090402[农学-农业昆虫与害虫防治] 

基　　金：financial support provided by the National Key Research and Development Program of China(2017YFD0100600) the National Natural Science Foundation of China(31471491 and 31871621) the Chinese Academy of Agricultural Sciences(CAAS)Innovation Team the International Cooperation Project in the Innovative Engineering of CAAS(CAAS-XTCX2018-020-2) 

主　　题：Triticum aestivum Blumeria graminis f.sp.tritici Pm52 molecular mapping 

摘      要：Resistance to powdery mildew is an important trait of interest in many wheat breeding programs. The information on genes conferring resistance to powdery mildew in wheat cultivars is useful in parental selection. Winter wheat breeding line DH51302 derived from Liangxing 99 and cultivar Shimai 26 derived from Jimai 22 showed identical infection patterns against 13 isolates of Blumeria graminis f. sp. tritici(Bgt) that causes wheat powdery mildew. DH51302 and Shimai 26 were crossed to a powdery mildew susceptible cultivar Zhongzuo 9504 and the F2:3 families were used in molecular localization of the resistance genes. Fourteen polymorphic markers, which were linked to Pm52 from Liangxing 99, were used to establish the genetic linkage maps for the resistance genes Pm DH51302 and Pm SM26 in DH51302 and Shimai 26, respectively. These genes were placed in the same genetic interval where Pm52 resides. Analysis of gene-linked molecular markers indicated that Pm DH51302 and Pm SM26 differed from other powdery mildew resistance genes on chromosome arm 2 BL, such as Pm6, Pm33, Pm51, Ml Zec1, Ml AB10, and Pm64. Based on the results of reaction patterns to different Bgt isolates and molecular marker localization, together with the pedigree information, DH51302 and Shimai 26 carried the same gene, Pm52, which confers their resistance to powdery mildew.