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Particle size distribution and removal by a chemical-biological flocculation process

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Journal of Environmental Sciences 2007年第5期19卷 559-563页

作者： ZHANG Zhi-bin ZHAO Jian-fu XIA Si-qing liu chang-qing KANG Xing-sheng State Key Laboratory of Pollution Control and Resource Reuse Research College of Environmental Science and Engineering Tongji University Shanghai 200092 China College of Municipal and Environmental Engineering Shandong Jianzhu University Jinan 250101China Shandong Academy of Environmental Sciences Jinan 250100 China

The particle characterization from the influent and effluent of a chemical-biological flocculation （CBF） process was studied with a laser diffraction device. Water samples from a chemically enhanced primary treatment （CEPT） process and a primary sediment tank process were also analyzed for comparison. The results showed that CBF process was not only effective for both the big size particles and small size particles removal, but also the best particle removal process in the three processes. The results also indicated that CBF process was superior to CEPT process in the heavy metals removal. The high and non-selective removal for heavy metals might be closely related to its strong ability to eliminate small particles. Samples from different locations in CBF reactors showed that small particles were easier to aggregate into big ones and those disrupted flocs could properly flocculate again along CBF reactor because of the biological flocculation.

关键词： particle removal chemical-biological flocculation (CBF) chemically enhanced primary treatment (CEPT)

Cloning and Functional Analysis of Lycopene ε-Cyclase (IbLCYe) Gene from Sweetpotato, Ipomoea batatas (L.) Lam.

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Journal of Integrative Agriculture 2013年第5期12卷 773-780页

作者： YU Ling ZHAI Hong CHEN Wei HE Shao-zhen liu qing-chang Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis and Utilization Ministry of Education/College of Agriculture and BiotechnologyChina Agricultural University

This paper reported firstly successful cloning of lycopene ε-cyclase （lbLCYe） gene from sweetpotato, lpomoea batatas （L.） Lam. Using rapid amplification of cDNA ends （RACE）, lbLCYe gene was cloned from sweetpotato cv. Nongdafu 14 with high carotenoid content. The 1 805 bp cDNA sequence oflbLCYe gene contained a 1236 bp open reading frame （ORF） encoding a 411 amino acids polypeptide with a molecular weight of 47 kDa and an isoelectric point （pI） of 6.95. IbLCYe protein contained one potential lycopene ε-cyclase domain and one potential FAD （flavinadenine dinucleotide）/NAD（P）（nicotinamide adenine dinucleotide phosphate）-binding domain, indicating that this protein shares the typical characteristics of LCYe proteins. The gDNA oflbLCYe gene was 4 029 bp and deduced to contain 5 introns and 6 exons. Real-time quantitative PCR analysis revealed that the expression level of IbLCYe gene was significantly higher in the storage roots of Nongdafu 14 than those in the leaves and stems. Transgenic tobacco （cv. Wisconsin 38） expressing [bLCYe gene accumulated significantly more ^-carotene compared to the untransformed control plants. These results showed that lbLCYe gene has an important function for the accumulation of carotenoids of sweetpotato.

关键词： IbLCYe gene cloning functional analysis carotenoid lpomoea batatas (L.) Lam.

Enhanced Stem Nematode Resistance of Transgenic Sweetpotato Plants Expressing Oryzacystatin-I Gene

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Agricultural Sciences in China 2011年第4期10卷 519-525页

作者： GAO Shang YU Bo ZHAI Hong HE Shao-zhen liu qing-chang Key Laboratory of Crop Genomics and Genetic Improvement Ministry of Agriculture/Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis and Utilization Ministry of Education~China Agricultural University Beijing 100193 P.R.China

Enhanced stem nematode resistance of transgenic sweetpotato （cv. Lizixiang） was achieved using Oryzacystatin-I （OCI） gene with Agrobacterium tumefaciens-mediated transformation. A. tumefaciens strain EHA105 harbors a binary vector pCAMBIA1301 with OCI gene, gusA gene and hptII gene. Selection culture was conducted using 25 mg L-1 hygromycin. A total of 1 715 plants were produced from the inoculated 1 450 cell aggregates of Lizixiang via somatic embryogenesis. GUS assay and PCR analysis of the putative transgenic plants randomly sampled showed that 90.54% of them were transgenic plants. Transgenic plants exhibited significantly enhanced resistance to stem nematodes compared to the untransformed control plants by the field evaluation with stem nematodes. Stable integration of the OCI gene into the genome of resistant transgenic plants was confirmed by Southern blot analysis, and the copy number of integrated OCI gene ranged from 1 to 4. Transgene overexpression in stem nematode-resistant plants was demonstrated by quantitative real-time PCR analysis. This study provides a way for improving stem nematode resistance in sweetpotato.

关键词： Agrobacterium tumefaciens Ipomoea batatas (L.) Lam. Oryzacystatin-I gene stem nematode resistance transgenic plant

Overexpression of SOS Genes Enhanced Salt Tolerance in Sweetpotato

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Journal of Integrative Agriculture 2012年第3期11卷 378-386页

作者： GAO Shang YUAN Li ZHAI Hong liu Cheng-long HE Shao-zhen liu qing-chang Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis and Utilization Ministry of Education/College of Agriculture and BiotechnologyChina

The production of transgenic sweetpotato （*** 18） plants exhibiting enhanced salt tolerance using salt overly sensitive （SOS） genes was achieved through Agrobacterium tumefaciens-mediated *** strain EHA105 harbors a binary vector pCAMBIA3301 with SOS genes （SOS1,SOS2 and SOS3） and bar *** culture was conducted using 0.3 mg L^-1 phosphinothricin （PPT）.A total of 40 plants were produced from the inoculated 170 cell aggregates via somatic *** analysis showed that 37 of the 40 regenerated plants were transgenic *** in vitro assay demonstrated that superoxide dismutase （SOD） and proline were significantly more accumulated and malonaldehyde （MDA） was significantly less accumulated in 21 transgenic plants than in control plants when they were exposed to 86 mmol L^-1 *** tolerance of these 21 plants was further evaluated with Hoagland solution containing 0,51,86,and 120 mmol L^-1 NaCl in the *** results indicated that 6 of them had significantly better growth and rooting ability than the remaining 15 transgenic plants and control *** of SOS genes in the 6 salt-tolerant transgenic plants was demonstrated by RT-PCR *** study provides an alternative approach for improving salt tolerance of sweetpotato.

关键词： Agrobacterium tumefaciens sweetpotato salt tolerance SOS transgenic plant

Identification of Differentially Expressed Genes in Sweetpotato Storage Roots Between Kokei No.14 and Its Mutant Nongdafu 14 Using PCR-Based cDNA Subtraction

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Journal of Integrative Agriculture 2013年第4期12卷 589-595页

作者： CHEN Wei ZHAI Hong YANG Yuan-jun HE Shao-zhen liu De-gao liu qing-chang Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis and Utilization Ministry of Education/China Agricultural University

The contents of earotenoids in the storage root of sweetpotato, Ipomoea batatas （L.） Lam. vary dramatically among different cultivars. However, so far little is known about the regulation of carotenoids synthesis in sweetpotato. In our laboratory, we identified a novel sweetpotato mutant, Nongdafu 14, which is a homogenous mutant derived from the wild type Kokei No. 14. The contents of carotenoids in the storage root of Nongdafu 14 were analyzed using high performance liquid chromatography （HPLC）, and it was found that the amount of carotenoids, [3-carotene, lutein and zeaxantion, three major types of earotenoids in sweetpotato storage roots, increased 2-26 folds in Nongdafu 14 compared to Kokei No. 14. Suppression subtractive hybridization （SSH） was used to identify genes that were differentially expressed in Nongdafu 14, and a differentially expressed eDNA library was constructed using the eDNA of Nongdafu 14 storage roots as tester and that of Kokei No. 14 storage roots as driver. Out of the 1 530 clones sequenced, we identified 292 nonredundant ESTs. GO and KEGG analyses of these differentially expressed ESTs indicated that diverse metabolism pathways were affected and candidate genes involved in regulation of carotenoids synthesis are suggested.

关键词： lpomoea batatas (L.) Lam. carotenoids suppression subtractive hybridization differentially expressed ESTs

Genetic diversity and population structure of 288 potato(Solanum tuberosum L.) germplasms revealed by SSR and AFLP markers

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Journal of Integrative Agriculture 2017年第11期16卷 2434-2443页

作者： WANG Jian HOU Lu WANG Ruo-yu HE Miao-miao liu qing-chang Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis and Utilization Ministry of EducationChina Agricultural University Institute of Biotechnology Qinghai Academy of Agriculture and Forestry Science Scientific Observing and Experimental Station of Crop Pest in Xining Ministry of Agriculture/Key Laboratory of Agricultural Integrated Pest ManagementQinghai Academy of Agriculture and Forestry Science Department of Statistics University of Connecticut

Potato （Solanum tuberosum L.） is an important staple food and economic crop in many countries. China has led world potato production in recent years. To understand the genetic diversity of potato germplasms and to enrich the current gene pool for potato improvement, we made a global collection consisted of 288 potato germplasms from eight countries and the International Potato Center （CIP）. Using SSR and AFLP techniques, we evaluated the genetic diversity and population structure of these 288 potato accessions. A total of 190 alleles on 20 SSR loci were detected and all of the SSR alleles were polymorphic among these potato germplasms with an average of 9.5 alleles per SSR locus ranging from 2 to 23. The effective number of alleles per locus （Ne＊）, Nei＇s genetic diversity （H＊）, and Shannon＇s information index （I＊） was from （0.1709＋0.3698） to （1.6166＋0.3414）, （0.076＋0.1388） to （0.3812＋0.1886）, and （0.1324＋0.1970） to （0.5347＋0.1440）, respec- tively, and the mean polymorphic information content （PIC） value was 0.7312. A total of 988 AFLP alleles were detected by 10 AFLP primer combinations with 983 polymorphic alleles, and 99.49% alleles was polymorphic with an average of 98.3 polymorphic alleles per primer combination ranging from 91 to 116. The values of Ne＊, H＊ and/＊ were from （1.5162＋0.311 ） to （1.6423＋0.3278）, （0.3114＋0.145） to （0.3675＋0.1121）, and （0.4761＋0.1792） to （0.547＋0.1322）, respectively, and the average PIC value was 0.9871. Bayesian analysis discriminated the accessions into seven subgroup and an admix group. The majority of accessions from CIP and China were assigned into SG1, SG5, SG6, SG7 and admix group. Accessions in SG3 were mainly from CIP and two small groups SG2 and SG4 were mainly from northeastern China. In general, the results obtained from Bayesian statistical analysis, cluster analysis and principal coordinate analysis consistently revealed the lack of geographical differentiation among country-wide collections, indicating germplasm introdu

关键词： potato germplasms genetic diversity population structure SSR AFLP

A geranylgeranyl pyrophosphate synthase gene, Ib GGPS, increases carotenoid contents in transgenic sweetpotato

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Journal of Integrative Agriculture 2022年第9期21卷 2538-2546页

作者： LI Rui-jie ZHAI Hong HE Shao-zhen ZHANG Huan ZHAO Ning liu qing-chang Key Laboratory of Sweetpotato Biology and Biotechnology Ministry of Agriculture and Rural Affairs/Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis and UtilizationMinistry of Education/College of Agronomy&BiotechnologyChina Agricultural UniversityBeijing 100193P.R.China

Geranylgeranyl pyrophosphate synthase(GGPS) plays an important role in the biosynthesis of carotenoids. In a previous study, the IbGGPS gene was isolated from a sweetpotato, Ipomoea batatas(L.) Lam., line Nongdafu 14 with high carotenoid contents, but its role and underlying mechanisms in carotenoid biosynthesis in sweetpotato were not investigated. In the present study, the IbGGPS gene was introduced into a sweetpotato cv. Lizixiang and the contents of β-carotene, β-cryptoxanthin, zeaxanthin and lutein were significantly increased in the storage roots of the IbGGPSoverexpressing sweetpotato plants. Further analysis showed that IbGGPS gene overexpression systematically upregulated the genes involved in the glycolytic, 2-C-methyl-D-erythritol-4-phosphate(MEP) and carotenoid pathways,which increased the carotenoid contents in the transgenic plants. These results indicate that the IbGGPS gene has the potential for use in improving the carotenoid contents in sweetpotato and other plants.

关键词： sweetpotato Ipomoea batatas(L.)Lam. IbGGPS overexpression carotenoid

Molecular Cloning and Functional Characterization of a Salt Tolerance-Associated Gene IbNFU1 from Sweetpotato

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Journal of Integrative Agriculture 2013年第1期12卷 27-35页

作者： WANG Lian-jun HE Shao-zhen ZHAI Hong liu De-gao WANG Yan-nan liu qing-chang Key Laboratory of Crop Genomics and Genetic Improvement Ministry of Agriculture/Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis and UtilizationMinistry of Education/China Agricultural University

Iron-sulfur cluster biosynthesis involving the nitrogen fixation（Nif） proteins has been proposed as a general mechanism acting in various ***-like protein may play an important role in protecting plants against abiotic and biotic *** on the EST sequence selected from salt-stressed suppression subtractive hybridization（SSH） cDNA library constructed with a salt-tolerant mutant LM79,a NFU gene,termed IbNFU1,was cloned from sweetpotato（Ipomoea batatas（L.） Lam.） via rapid amplification of cDNA ends（RACE）.The cDNA sequence of 1 117 bp contained an 846 bp open reading frame encoding a 281 amino acids polypeptide with a molecular weight of 30.5 kDa and an isoelectric point（pI） of ***1 gene contained a conserved Cys-X-X-Cys motif in C-terminal of the iron-sulfur cluster *** deduced amino acid sequence had 66.08 to 71.99% sequence identity to NFU genes reported in Arabidopsis thaliana,Eucalyptus grandis and Vitis ***-time quantitative PCR analysis revealed that the expression level of IbNFU1 gene was significantly higher in the roots of the mutant LM79 compared to the wild-type *** tobacco（*** 38） plants expressing IbNFU1 gene exhibited significantly higher salt tolerance compared to the untransformed control *** is proposed that IbNFU1 gene has an important function for salt tolerance of plants.

关键词： cloning IbNFU1 gene Ipomoea batatas(L.) Lam. salt tolerance

Analysis of drought tolerance and genetic and epigenetic variations in a somatic hybrid between Ipomoea batatas (L.) Lam. and I. triloba L.

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Journal of Integrative Agriculture 2017年第1期16卷 36-46页

作者： JIA Li-cong ZHAI Hong HE Shao-zhen YANG Yu-feng liu qing-chang Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis and Utilization Ministry of Education/China Agricultural University Beijing 100193 P.R. China

The somatic hybrid KT1 was previously obtained from protoplast fusion between sweetpotato （Ipomoea batatas （L.） Lam.） cv. Kokei No. 14 and its wild relative I. triloba L. However, its genetic and epigenetic variations have not been investigated. This study showed that KT1 exhibited significantly higher drought tolerance compared to the cultivated parent Kokei No. 14. The content of proline and activities of superoxide dismutase （SOD） and photosynthesis were significantly increased, while malonaldehyde （MDA） content was significantly decreased compared to Kokei No. 14 under drought stress. KT1 also showed higher expression level of well-known drought stress-responsive genes compared to Kokei No. 14 under drought stress. Amplified fragment length polymorphism （AFLP） and methylation-sensitive amplified polymorphism （MSAP） analyses indicated that KT1 had AFLP and MSAP band patterns consisting of both parent specific bands and changed bands. Fur- ther analysis demonstrated that in KT1. the proportions of Kokei No. 14 specific genome components and methylation sites were much greater than those of I. triloba. KT1 had the same chloroplast and mitochondrial genomes as Kokei No. 14. These results will aid in developing the useful genes ofI. triloba and understanding the evolution and phylogeny of the cultivated sweetpotato.

关键词： drought tolerance epigenetic variation genetic variation Ipomoea batatas (L.) Lain. I. triloba L. somatic hybrid