Genetic diversity and population structure of 288 potato(Solanum tuberosum L.) germplasms revealed by SSR and AFLP markers

作     者：WANG Jian HOU Lu WANG Ruo-yu HE Miao-miao LIU Qing-chang 

作者机构：Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis and UtilizationMinistry of EducationChina Agricultural University Institute of BiotechnologyQinghai Academy of Agriculture and Forestry Science Scientific Observing and Experimental Station of Crop Pest in XiningMinistry of Agriculture/Key Laboratory of Agricultural Integrated Pest ManagementQinghai Academy of Agriculture and Forestry Science Department of StatisticsUniversity of Connecticut 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2017年第16卷第11期

页      面：2434-2443页

核心收录：

学科分类：09[农学] 0901[农学-作物学] 

基　　金：supported by the earmarked fund for China Agriculture Research System(CARS-10) 

主　　题：potato germplasms genetic diversity population structure SSR AFLP 

摘      要：Potato （Solanum tuberosum L.） is an important staple food and economic crop in many countries. China has led world potato production in recent years. To understand the genetic diversity of potato germplasms and to enrich the current gene pool for potato improvement, we made a global collection consisted of 288 potato germplasms from eight countries and the International Potato Center （CIP）. Using SSR and AFLP techniques, we evaluated the genetic diversity and population structure of these 288 potato accessions. A total of 190 alleles on 20 SSR loci were detected and all of the SSR alleles were polymorphic among these potato germplasms with an average of 9.5 alleles per SSR locus ranging from 2 to 23. The effective number of alleles per locus （Ne＊）, Nei＇s genetic diversity （H＊）, and Shannon＇s information index （I＊） was from （0.1709＋0.3698） to （1.6166＋0.3414）, （0.076＋0.1388） to （0.3812＋0.1886）, and （0.1324＋0.1970） to （0.5347＋0.1440）, respec- tively, and the mean polymorphic information content （PIC） value was 0.7312. A total of 988 AFLP alleles were detected by 10 AFLP primer combinations with 983 polymorphic alleles, and 99.49% alleles was polymorphic with an average of 98.3 polymorphic alleles per primer combination ranging from 91 to 116. The values of Ne＊, H＊ and/＊ were from （1.5162＋0.311 ） to （1.6423＋0.3278）, （0.3114＋0.145） to （0.3675＋0.1121）, and （0.4761＋0.1792） to （0.547＋0.1322）, respectively, and the average PIC value was 0.9871. Bayesian analysis discriminated the accessions into seven subgroup and an admix group. The majority of accessions from CIP and China were assigned into SG1, SG5, SG6, SG7 and admix group. Accessions in SG3 were mainly from CIP and two small groups SG2 and SG4 were mainly from northeastern China. In general, the results obtained from Bayesian statistical analysis, cluster analysis and principal coordinate analysis consistently revealed the lack of geographical differentiation among country-wide collections, indicating germplasm introdu