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白花草木樨MaERF058基因耐旱功能验证

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草业学报 2024年第8期33卷 159-169页

作者：魏娜敬文茂许尔文王荣新赵晶忠马雪娥张吉宇刘文献甘肃省祁连山水源涵养林研究院甘肃张掖734000 兰州大学草种创新与草地农业生态系统全国重点实验室甘肃兰州730030 甘肃祁连山森林生态系统国家定位观测研究站甘肃张掖734000

干旱是农业生产中最常见的逆境胁迫之一,对植物的生长发育、光合作用、气孔开闭、渗透功能和激素调节等方面都会产生不良影响。白花草木樨在我国北方干旱地区广泛种植,是具有耐旱、耐盐碱、耐贫瘠和粗蛋白含量高等特点的优质牧草。转录... 详细信息

干旱是农业生产中最常见的逆境胁迫之一,对植物的生长发育、光合作用、气孔开闭、渗透功能和激素调节等方面都会产生不良影响。白花草木樨在我国北方干旱地区广泛种植,是具有耐旱、耐盐碱、耐贫瘠和粗蛋白含量高等特点的优质牧草。转录因子(TF)作为调控基因表达的重要蛋白分子,可作用于其下游启动子区的特定顺式元件以激活或抑制基因的转录,进而调控植物生长发育及响应逆境。植物ERF转录因子家族基因参与植物对非生物胁迫的抗性,是作物抗逆性改良的理想候选基因。本研究对白花草木樨关键耐旱基因MaERF058进行烟草亚细胞定位分析,发现该基因表达蛋白定位于细胞核。干旱胁迫下,过表达MaERF058的拟南芥长势明显优于野生型。干旱条件下转MaERF058基因白花草木樨毛状根脯氨酸含量较对照显著(P<0.01)增加;丙二醛含量显著(P<0.01)降低;过氧化氢酶活性显著(P<0.05)增加;单胺氧化酶染色(NBT法)结果显示转基因毛状根的活性氧含量低于对照。以上结果表明白花草木樨MaERF058基因编码的转录因子对调控白花草木樨响应干旱胁迫具有积极作用。研究结果为解析白花草木樨耐旱分子机制提供了新思路,同时也能够为加快耐旱牧草分子育种提供优异基因资源。

关键词：白花草木樨 ERF转录因子干旱胁迫遗传转化

Undersea safety mining of the large gold deposit in Xinli District of Sanshandao Gold Mine

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International Journal of Minerals,Metallurgy and Materials 2012年第7期19卷 574-583页

作者： Zhi-xiang liu wen-gang Dang xian-qun He School of Resources and Safety Engineering Central South University Changsha 410083 China

The exploration of undersea resources becomes popular as land resources decrease. Researches were conducted with emphasis on the safety and efficiency of undersea mining of the large gold deposit in Xinli District of Sanshandao Gold Mine. A series of tests for the physical and mechanical characteristics of rock mass were carried out, and the three-dimensional geo-stress distribution was tested in the mining area. Further, a similar experimental simulation platform, which revealed the mechanism of water inrush and ascertained the reason- able thickness of the safety isolate layer, was established for the undersea mining. Meanwhile, the feasibility of cancelling the ore pillars and the safety conditions was checked by numerical simulation. The simulation results show that it is safe to exploit the ore body below the -85 m level （presently, the exploitation level is below -160 m in Xinli District）, and the ore pillars can be cancelled below the -560 m level. Fur- thermore, a novel backfill method was designed to reduce the rock strata disturbance and settlement, and the settlement of roof strata was monitored during the mining process. Engineering practice shows that the settlement of roof strata was small and that no disaster happened. This indicates that the undersea safety mining technology of the large gold deposit is achieved in Xinli District.

关键词： undersea mining gold deposits rock mechanics mine roof control computer simulation

Kiwifruit(Actinidia chinensis)R1R2R3-MYB transcription factor AcMYB3R enhances drought and salinity tolerance in Arabidopsis thaliana

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Journal of Integrative Agriculture 2019年第2期18卷 417-427页

作者： ZHANG Ya-bin TANG Wei WANG Li-huan HU Ya-wen liu xian-wen liu Yong-sheng Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education College of Life SciencesSichuan UniversityChengdu 610065P.R.China School of Food Science and Engineering Hefei University of TechnologyHefei 230009P.R.China

Kiwifruit is an important fruit crop that is highly sensitive to environmental stresses,such as drought,heat,cold,water logging and *** it is indispensable to identify stress-responsive candidate genes in kiwifruit cultivars for the stress resistance *** we report the isolation and characterization of a novel kiwifruit R1R2R3-MYB homolog(AcMYB3R)whose expression was induced by drought,salinity and cold *** vitro assays showed that AcMYB3R is a nuclear protein with transcriptional activation activity by binding to the cis-element of the kiwifruit orthologue of G2/M phase-specific gene *** Arabidopsis transgenic plants overexpressing AcMYB3R showed drastically enhanced tolerance to drought and salt *** expressions of stress-responsive genes such as RD29A,RD29B,COR15A and RD22 were prominently up-regulated by ectopic expression of Ac *** study provides a valuable piece of information for functional genomics studies of kiwifruit and molecular breeding in improving stress tolerance for crop production.

关键词： kiwifruit R1R2R3-MYB drought abiotic stress

Chinese Medicine in Treatment of A Patient with Acute Extensive Anterior Myocardial Infarction Complicated by Shock after Percutaneous Coronary Intervention

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Chinese Journal of Integrative Medicine 2019年第5期25卷 366-369页

作者： LI Shi-bao SHENG Jin ZHOU Qi XING wen-long liu Hong-xu liu wen-xian Department of Cardiovascular Beijing Hospital of Traditional Chinese MedicineCapital Medical UniversityBeijing(100010)China Department of Cardiac Care Unit Beijing Anzhen HospitalCapital Medical UniversityBeijing(100029)China

Acute myocardial infarction(AMI)is a clinical syndrome of myocardial ischemia and necrosis caused by acute blocking of the coronary *** complicated by cardiogenic shock(CS),it has an extremely high mortality.(1,2)Early reperfusion therapy combined with left ventricular assist device(LVAD)can raise the survival rate to 58.8%–60.9%.(3)We report a case of successful treatment with integrated Chinese and Western medicine on acute extensive anterior myocardial infarction complicated by refractory CS.

关键词： Chinese Medicine Infarction Shock

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专科医师规范化培训教学方式改革及效果评价

医院管理论坛 2024年第4期41卷 74-76页

作者：张宁陈立颖刘文娴首都医科大学附属北京安贞医院

目的分析心血管内科专培学员对案例教学联合团队教学方式的评价,评估教学方式改革的效果。方法将心血管内科专培学员随机分为教改组(案例教学联合团队教学)和对照组(传统讲授式教学),每组各17人。教改组采用案例教学联合团队教学,对照... 详细信息

目的分析心血管内科专培学员对案例教学联合团队教学方式的评价,评估教学方式改革的效果。方法将心血管内科专培学员随机分为教改组(案例教学联合团队教学)和对照组(传统讲授式教学),每组各17人。教改组采用案例教学联合团队教学,对照组采用传统讲授式教学。教改组学员接受问卷调查。两组学员均进行了理论知识和病例分析考试。结果专培学员认为案例教学联合团队教学更具优势、理论知识和病例分析考试得分也更高(p<0.05)。结论案例教学联合团队教学更具优势,可应用于心血管专培学员的教学。

关键词：专科医师规范化培训案例教学团队教学心血管专培学员

Analysis of Genetic Diversity in Natural Populations of Psathyrostachys huashanica Keng Using Microsatellite (SSR) Markers

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Agricultural Sciences in China 2010年第4期9卷 463-471页

作者： liu wen-xian liu Wei-hua WU Jun GAO Ai-nong LI Li-hui National Key Facility for Crop Gene Resources and Genetic Improvement （NFCRI） Institute of Crop Germplasm Resources Chinese Academy of Agricultural Sciences Beijing 100081 P.R.China Institute of Botany Chinese Academy of Sciences Beijing 100093 P.R.China Graduate School Chinese Academy of Sciences Beijing 100049 P.R.China College of Agronomy Northwest A＆F University Yangling 712100 P.R.China

Psathyrostachys huashanica Keng is endemic to China and only distributed in Huashan Mountain in Shaanxi Province, China. In this study, 15 P. huashanica populations consisting of 450 individuals sampled across their main distribution were investigated by using the simple sequence repeats （SSRs） markers. A total of 184 alleles were detected on 24 SSR loci, and the number of alleles on each locus ranged from 2 to15, with an average of 7.667. The total gene diversity （HT= 0.683） and the coefficient of population differentiation （GST = 0.125） showed that P. huashanica had a relatively high level of genetic variation, and the genetic variation was mainly distributed within the populations. The gene flow among the populations of P. huashanica （Nm = 1.750） was much less than that of the common anemophytes （Nm = 5.24）. Correlation analysis demonstrated that the number of alleles as well as genetic diversity of the five populations of Huangpu valley decreased along with the increase of altitudes, but the correlation was not significant. Implications of these results for future P. huashanica collection, evaluation and conservation were discussed.

关键词： Psathyrostachys huashanica Keng SSR genetic diversity conservation implication

Computational Generation and the Simulation of the Light Scattering of Self-Affine Fractal Random Surface

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Chinese Physics Letters 2001年第2期18卷 217-219页

作者： liu wen-xian TENG Shu-Yun ZHANG Ning-Yu liu De-Li CHENG Chuan-Fu Department of Physics Shandong Normal UniversityJi’nan 250014

We propose a method for the generation of self-affine fractal random surfaces,in which we use Fourier transform and its inversion in the *** light scattering of surfaces of this kind is simulated at different incident angles of *** variation of the full width at half maximum(FWHM)of the intensity profile versus the perpendicular component k_(⊥)of the wave-vector shows clearly the characteristics of the surfaces *** simulation demonstrates how the value of FWHM at k^(2)_(⊥)w^(2)≤1 region and the slope of ln wp-ln k_(⊥)curve at k^(2)_(⊥)w^(2)≥1 region are used,respectively,to extract the lateral correlation lengthξand the roughness exponentα.

关键词： scattering Fractal illumination

Recent advances in vacancy engineering for reliable lithiumsulfur batteries

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Rare Metals 2024年第2期43卷 455-477页

作者： wen-Dong liu Xiao Tang Jun-An Feng Chao-Yue Zhang Hao liu Chuan Shi Xiao-xian Zhao Jian-Jun Song College of Physics School of Chemistry and Chemical EngineeringQingdao UniversityQingdao266071China Faculty of Science Centre for Clean Energy TechnologySchool of Mathematical and Physical SciencesUniversity of Technology SydneySydneyNSW2007Australia Department of Chemistry College of ScienceHebei Agricultural UniversityBaoding071001China

The high-energy-density and low-cost features endow lithium-sulfur batteries with broad application ***,many drawbacks,especially the detrimental shuttle effect,have hindered the further development of *** response,a lot of new structures have been applied to suppress the shuttle effect and promote the development of ***,vacancy engineering has gained the attention of researchers due to its unique electronic *** review aims to analyze the application of vacancy engineering in ***,the electrochemistry of LSBs has been systematically discussed and the existing challenges as well as improvement tactics of LSBs have also been ***,the preparation methods and characterization technologies of various vacancies are summarized,including oxygen vacancies,sulfur vacancies,selenium vacancies,other anion vacancies,cation vacancies,*** latest applications of vacancy engineering in LSBs are also summarized in this ***,some prospects and insights for further investigation and practical application of vacancy engineering in LSBs are put forward.

关键词： Lithium-sulfur batteries(LSBs) Defects Vacancy engineering Electronic structure Shuttle effect

Hyperspectral image super resolution using deep internal and self-supervised learning

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CAAI Transactions on Intelligence Technology 2024年第1期9卷 128-141页

作者： Zhe liu xian-Hua Han Graduate School of Science and Technology for Innovation Yamaguchi UniversityYamaguchiJapan Graduate School of Artificial Intelligence and Science Rikkyo UniversityTokyoJapan

By automatically learning the priors embedded in images with powerful modelling ca-pabilities,deep learning-based algorithms have recently made considerable progress in reconstructing the high-resolution hyperspectral(HR-HS)*** previously collected large-amount of external data,these methods are intuitively realised under the full supervision of the ground-truth ***,the database construction in merging the low-resolution(LR)HS(LR-HS)and HR multispectral(MS)or RGB image research paradigm,commonly named as HSI SR,requires collecting corresponding training triplets:HR-MS(RGB),LR-HS and HR-HS image simultaneously,and often faces dif-ficulties in *** learned models with the training datasets collected simultaneously under controlled conditions may significantly degrade the HSI super-resolved perfor-mance to the real images captured under diverse *** handle the above-mentioned limitations,the authors propose to leverage the deep internal and self-supervised learning to solve the HSI SR *** authors advocate that it is possible to train a specific CNN model at test time,called as deep internal learning(DIL),by on-line preparing the training triplet samples from the observed LR-HS/HR-MS(or RGB)images and the down-sampled LR-HS ***,the number of the training triplets extracted solely from the transformed data of the observation itself is extremely few particularly for the HSI SR tasks with large spatial upscale factors,which would result in limited reconstruction *** solve this problem,the authors further exploit deep self-supervised learning(DSL)by considering the observations as the unlabelled training ***,the degradation modules inside the network were elaborated to realise the spatial and spectral down-sampling procedures for transforming the generated HR-HS estimation to the high-resolution RGB/LR-HS approximation,and then the reconstruction errors of the observations were formulated for mea

关键词： computer vision deep learning deep neural networks hyperspectral image enhancement