Analysis of Genetic Diversity in Natural Populations of Psathyrostachys huashanica Keng Using Microsatellite (SSR) Markers

作     者：LIU Wen-xian LIU Wei-hua WU Jun GAO Ai-nong LI Li-hui 

作者机构：National Key Facility for Crop Gene Resources and Genetic Improvement （NFCRI） Institute of Crop Germplasm Resources Chinese Academy of Agricultural Sciences Beijing 100081 P.R.China Institute of Botany Chinese Academy of Sciences Beijing 100093 P.R.China Graduate School Chinese Academy of Sciences Beijing 100049 P.R.China College of Agronomy Northwest A＆F University Yangling 712100 P.R.China 

出 版 物：《Agricultural Sciences in China》 (中国农业科学（英文版）)

年 卷 期：2010年第9卷第4期

页      面：463-471页

核心收录：

学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学] 09[农学] 0901[农学-作物学] 0902[农学-园艺学] 090202[农学-蔬菜学] 

基　　金：support wasprovided by the Ministry of Science and Technology of China (2004DIB3J090) 

主　　题：Psathyrostachys huashanica Keng SSR genetic diversity conservation implication 

摘      要：Psathyrostachys huashanica Keng is endemic to China and only distributed in Huashan Mountain in Shaanxi Province, China. In this study, 15 P. huashanica populations consisting of 450 individuals sampled across their main distribution were investigated by using the simple sequence repeats （SSRs） markers. A total of 184 alleles were detected on 24 SSR loci, and the number of alleles on each locus ranged from 2 to15, with an average of 7.667. The total gene diversity （HT= 0.683） and the coefficient of population differentiation （GST = 0.125） showed that P. huashanica had a relatively high level of genetic variation, and the genetic variation was mainly distributed within the populations. The gene flow among the populations of P. huashanica （Nm = 1.750） was much less than that of the common anemophytes （Nm = 5.24）. Correlation analysis demonstrated that the number of alleles as well as genetic diversity of the five populations of Huangpu valley decreased along with the increase of altitudes, but the correlation was not significant. Implications of these results for future P. huashanica collection, evaluation and conservation were discussed.