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Absorption Cross Section of Static Einstein-Maxwell Dilation Axion Black Hole for Scalar Particles

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Communications in Theoretical Physics 2007年第4期47卷 665-668页

作者： liu chang-qing JING Ji-Liang Institute of Physics and Department of Physics Hunan Normal University Changsha 410081 China

The absorption cross section of the static Einstein-Maxwell dilaton axion （EMDA） black hole for scalar particles is investigated. It is shown that the ratio of the absorption cross section of the EMDA black hole to that of the Schwarzschild black hole decreases as the absolute value of the dilaton increases, and it becomes zero as the dilaton tends to its extremal value. It is also shown that the absorption cross section decreases as both the v and the absolute value of the dilaton increase, and it decreases as the mass of the particle decreases.

关键词： absorption cross section black hole dilaton

P-V Criticality of an AdS Black Hole in f(R)Gravity

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Chinese Physics Letters 2013年第6期30卷 38-41页

作者： CHEN Song-Bai liu Xiao-Fang liu chang-qing Institute of Physics and Department of Physics Hunan Normal UniversityChangsha 410081 Key Laboratory of Low Dimensional Quantum Structures and Quantum Control Ministry of EducationHunan Normal UniversityChangsha 410081

We study the thermodynamics of a charged AdS black hole in the special f(R)correction with the constant Ricci scalar *** results show that the f(R)correction influences the Gibbs free energy and the phase transition of *** ratioρc occurring at the critical point increases monotonically with the derivative term f'(R0).We also disclose that the critical exponents are the same as those of the liquid-gas phase transition in the van der Waals model,which does not depend on the f(R)correction considered here.

关键词： correction (R) critical

Particle size distribution and removal by a chemical-biological flocculation process

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Journal of Environmental Sciences 2007年第5期19卷 559-563页

作者： ZHANG Zhi-bin ZHAO Jian-fu XIA Si-qing liu chang-qing KANG Xing-sheng State Key Laboratory of Pollution Control and Resource Reuse Research College of Environmental Science and Engineering Tongji University Shanghai 200092 China College of Municipal and Environmental Engineering Shandong Jianzhu University Jinan 250101China Shandong Academy of Environmental Sciences Jinan 250100 China

The particle characterization from the influent and effluent of a chemical-biological flocculation （CBF） process was studied with a laser diffraction device. Water samples from a chemically enhanced primary treatment （CEPT） process and a primary sediment tank process were also analyzed for comparison. The results showed that CBF process was not only effective for both the big size particles and small size particles removal, but also the best particle removal process in the three processes. The results also indicated that CBF process was superior to CEPT process in the heavy metals removal. The high and non-selective removal for heavy metals might be closely related to its strong ability to eliminate small particles. Samples from different locations in CBF reactors showed that small particles were easier to aggregate into big ones and those disrupted flocs could properly flocculate again along CBF reactor because of the biological flocculation.

关键词： particle removal chemical-biological flocculation (CBF) chemically enhanced primary treatment (CEPT)

Cloning and Functional Analysis of Lycopene ε-Cyclase (IbLCYe) Gene from Sweetpotato, Ipomoea batatas (L.) Lam.

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Journal of Integrative Agriculture 2013年第5期12卷 773-780页

作者： YU Ling ZHAI Hong CHEN Wei HE Shao-zhen liu qing-chang Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis and Utilization Ministry of Education/College of Agriculture and BiotechnologyChina Agricultural University

This paper reported firstly successful cloning of lycopene ε-cyclase （lbLCYe） gene from sweetpotato, lpomoea batatas （L.） Lam. Using rapid amplification of cDNA ends （RACE）, lbLCYe gene was cloned from sweetpotato cv. Nongdafu 14 with high carotenoid content. The 1 805 bp cDNA sequence oflbLCYe gene contained a 1236 bp open reading frame （ORF） encoding a 411 amino acids polypeptide with a molecular weight of 47 kDa and an isoelectric point （pI） of 6.95. IbLCYe protein contained one potential lycopene ε-cyclase domain and one potential FAD （flavinadenine dinucleotide）/NAD（P）（nicotinamide adenine dinucleotide phosphate）-binding domain, indicating that this protein shares the typical characteristics of LCYe proteins. The gDNA oflbLCYe gene was 4 029 bp and deduced to contain 5 introns and 6 exons. Real-time quantitative PCR analysis revealed that the expression level of IbLCYe gene was significantly higher in the storage roots of Nongdafu 14 than those in the leaves and stems. Transgenic tobacco （cv. Wisconsin 38） expressing [bLCYe gene accumulated significantly more ^-carotene compared to the untransformed control plants. These results showed that lbLCYe gene has an important function for the accumulation of carotenoids of sweetpotato.

关键词： IbLCYe gene cloning functional analysis carotenoid lpomoea batatas (L.) Lam.

Enhanced Stem Nematode Resistance of Transgenic Sweetpotato Plants Expressing Oryzacystatin-I Gene

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Agricultural Sciences in China 2011年第4期10卷 519-525页

作者： GAO Shang YU Bo ZHAI Hong HE Shao-zhen liu qing-chang Key Laboratory of Crop Genomics and Genetic Improvement Ministry of Agriculture/Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis and Utilization Ministry of Education~China Agricultural University Beijing 100193 P.R.China

Enhanced stem nematode resistance of transgenic sweetpotato （cv. Lizixiang） was achieved using Oryzacystatin-I （OCI） gene with Agrobacterium tumefaciens-mediated transformation. A. tumefaciens strain EHA105 harbors a binary vector pCAMBIA1301 with OCI gene, gusA gene and hptII gene. Selection culture was conducted using 25 mg L-1 hygromycin. A total of 1 715 plants were produced from the inoculated 1 450 cell aggregates of Lizixiang via somatic embryogenesis. GUS assay and PCR analysis of the putative transgenic plants randomly sampled showed that 90.54% of them were transgenic plants. Transgenic plants exhibited significantly enhanced resistance to stem nematodes compared to the untransformed control plants by the field evaluation with stem nematodes. Stable integration of the OCI gene into the genome of resistant transgenic plants was confirmed by Southern blot analysis, and the copy number of integrated OCI gene ranged from 1 to 4. Transgene overexpression in stem nematode-resistant plants was demonstrated by quantitative real-time PCR analysis. This study provides a way for improving stem nematode resistance in sweetpotato.

关键词： Agrobacterium tumefaciens Ipomoea batatas (L.) Lam. Oryzacystatin-I gene stem nematode resistance transgenic plant

Overexpression of SOS Genes Enhanced Salt Tolerance in Sweetpotato

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Journal of Integrative Agriculture 2012年第3期11卷 378-386页

作者： GAO Shang YUAN Li ZHAI Hong liu Cheng-long HE Shao-zhen liu qing-chang Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis and Utilization Ministry of Education/College of Agriculture and BiotechnologyChina

The production of transgenic sweetpotato （*** 18） plants exhibiting enhanced salt tolerance using salt overly sensitive （SOS） genes was achieved through Agrobacterium tumefaciens-mediated *** strain EHA105 harbors a binary vector pCAMBIA3301 with SOS genes （SOS1,SOS2 and SOS3） and bar *** culture was conducted using 0.3 mg L^-1 phosphinothricin （PPT）.A total of 40 plants were produced from the inoculated 170 cell aggregates via somatic *** analysis showed that 37 of the 40 regenerated plants were transgenic *** in vitro assay demonstrated that superoxide dismutase （SOD） and proline were significantly more accumulated and malonaldehyde （MDA） was significantly less accumulated in 21 transgenic plants than in control plants when they were exposed to 86 mmol L^-1 *** tolerance of these 21 plants was further evaluated with Hoagland solution containing 0,51,86,and 120 mmol L^-1 NaCl in the *** results indicated that 6 of them had significantly better growth and rooting ability than the remaining 15 transgenic plants and control *** of SOS genes in the 6 salt-tolerant transgenic plants was demonstrated by RT-PCR *** study provides an alternative approach for improving salt tolerance of sweetpotato.

关键词： Agrobacterium tumefaciens sweetpotato salt tolerance SOS transgenic plant

Identification of Differentially Expressed Genes in Sweetpotato Storage Roots Between Kokei No.14 and Its Mutant Nongdafu 14 Using PCR-Based cDNA Subtraction

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Journal of Integrative Agriculture 2013年第4期12卷 589-595页

作者： CHEN Wei ZHAI Hong YANG Yuan-jun HE Shao-zhen liu De-gao liu qing-chang Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis and Utilization Ministry of Education/China Agricultural University

The contents of earotenoids in the storage root of sweetpotato, Ipomoea batatas （L.） Lam. vary dramatically among different cultivars. However, so far little is known about the regulation of carotenoids synthesis in sweetpotato. In our laboratory, we identified a novel sweetpotato mutant, Nongdafu 14, which is a homogenous mutant derived from the wild type Kokei No. 14. The contents of carotenoids in the storage root of Nongdafu 14 were analyzed using high performance liquid chromatography （HPLC）, and it was found that the amount of carotenoids, [3-carotene, lutein and zeaxantion, three major types of earotenoids in sweetpotato storage roots, increased 2-26 folds in Nongdafu 14 compared to Kokei No. 14. Suppression subtractive hybridization （SSH） was used to identify genes that were differentially expressed in Nongdafu 14, and a differentially expressed eDNA library was constructed using the eDNA of Nongdafu 14 storage roots as tester and that of Kokei No. 14 storage roots as driver. Out of the 1 530 clones sequenced, we identified 292 nonredundant ESTs. GO and KEGG analyses of these differentially expressed ESTs indicated that diverse metabolism pathways were affected and candidate genes involved in regulation of carotenoids synthesis are suggested.

关键词： lpomoea batatas (L.) Lam. carotenoids suppression subtractive hybridization differentially expressed ESTs

Genetic diversity and population structure of 288 potato(Solanum tuberosum L.) germplasms revealed by SSR and AFLP markers

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Journal of Integrative Agriculture 2017年第11期16卷 2434-2443页

作者： WANG Jian HOU Lu WANG Ruo-yu HE Miao-miao liu qing-chang Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis and Utilization Ministry of EducationChina Agricultural University Institute of Biotechnology Qinghai Academy of Agriculture and Forestry Science Scientific Observing and Experimental Station of Crop Pest in Xining Ministry of Agriculture/Key Laboratory of Agricultural Integrated Pest ManagementQinghai Academy of Agriculture and Forestry Science Department of Statistics University of Connecticut

Potato （Solanum tuberosum L.） is an important staple food and economic crop in many countries. China has led world potato production in recent years. To understand the genetic diversity of potato germplasms and to enrich the current gene pool for potato improvement, we made a global collection consisted of 288 potato germplasms from eight countries and the International Potato Center （CIP）. Using SSR and AFLP techniques, we evaluated the genetic diversity and population structure of these 288 potato accessions. A total of 190 alleles on 20 SSR loci were detected and all of the SSR alleles were polymorphic among these potato germplasms with an average of 9.5 alleles per SSR locus ranging from 2 to 23. The effective number of alleles per locus （Ne＊）, Nei＇s genetic diversity （H＊）, and Shannon＇s information index （I＊） was from （0.1709＋0.3698） to （1.6166＋0.3414）, （0.076＋0.1388） to （0.3812＋0.1886）, and （0.1324＋0.1970） to （0.5347＋0.1440）, respec- tively, and the mean polymorphic information content （PIC） value was 0.7312. A total of 988 AFLP alleles were detected by 10 AFLP primer combinations with 983 polymorphic alleles, and 99.49% alleles was polymorphic with an average of 98.3 polymorphic alleles per primer combination ranging from 91 to 116. The values of Ne＊, H＊ and/＊ were from （1.5162＋0.311 ） to （1.6423＋0.3278）, （0.3114＋0.145） to （0.3675＋0.1121）, and （0.4761＋0.1792） to （0.547＋0.1322）, respectively, and the average PIC value was 0.9871. Bayesian analysis discriminated the accessions into seven subgroup and an admix group. The majority of accessions from CIP and China were assigned into SG1, SG5, SG6, SG7 and admix group. Accessions in SG3 were mainly from CIP and two small groups SG2 and SG4 were mainly from northeastern China. In general, the results obtained from Bayesian statistical analysis, cluster analysis and principal coordinate analysis consistently revealed the lack of geographical differentiation among country-wide collections, indicating germplasm introdu

关键词： potato germplasms genetic diversity population structure SSR AFLP

A geranylgeranyl pyrophosphate synthase gene, Ib GGPS, increases carotenoid contents in transgenic sweetpotato

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Journal of Integrative Agriculture 2022年第9期21卷 2538-2546页

作者： LI Rui-jie ZHAI Hong HE Shao-zhen ZHANG Huan ZHAO Ning liu qing-chang Key Laboratory of Sweetpotato Biology and Biotechnology Ministry of Agriculture and Rural Affairs/Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis and UtilizationMinistry of Education/College of Agronomy&BiotechnologyChina Agricultural UniversityBeijing 100193P.R.China

Geranylgeranyl pyrophosphate synthase(GGPS) plays an important role in the biosynthesis of carotenoids. In a previous study, the IbGGPS gene was isolated from a sweetpotato, Ipomoea batatas(L.) Lam., line Nongdafu 14 with high carotenoid contents, but its role and underlying mechanisms in carotenoid biosynthesis in sweetpotato were not investigated. In the present study, the IbGGPS gene was introduced into a sweetpotato cv. Lizixiang and the contents of β-carotene, β-cryptoxanthin, zeaxanthin and lutein were significantly increased in the storage roots of the IbGGPSoverexpressing sweetpotato plants. Further analysis showed that IbGGPS gene overexpression systematically upregulated the genes involved in the glycolytic, 2-C-methyl-D-erythritol-4-phosphate(MEP) and carotenoid pathways,which increased the carotenoid contents in the transgenic plants. These results indicate that the IbGGPS gene has the potential for use in improving the carotenoid contents in sweetpotato and other plants.

关键词： sweetpotato Ipomoea batatas(L.)Lam. IbGGPS overexpression carotenoid