检索结果-南通市图书馆

Establishment of a selectable marker recycling system for iterative gene editing in Fusarium fujikuroi

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Synthetic and Systems Biotechnology 2024年第1期9卷 159-164页

作者： Tian-Qiong Shi cai-ling Yang Dong-Xun Li Yue-Tong Wang Zhi-Kui Nie Jiangxi New Reyphon Biochemical Co. LtdSalt&Chemical IndustryXinganJiangxi331399People’s Republic of China School of Food Science and Pharmaceutical Engineering Nanjing Normal University2 Xuelin RoadQixia DistrictNanjing210023People’s Republic of China

Gibberellic acid(GA3)is a vital plant growth hormone widely used in ***,GA3 production relies on liquid fermentation by the filamentous fungus Fusarium ***,the lack of an effective selection marker recycling system hampers the application of metabolic engineering technology in ***,as multiple-gene editing and positive-strain screening still rely on a limited number of *** this study,we developed a strategy using pyr4-blaster and CRISPR/Cas9 tools for recycling orotidine-5'-phosphate decarboxylase(Pyr4)selection *** demonstrated the effectiveness of this method for iterative gene integration and large gene-cluster *** also successfully improved GA3 titers by overexpressing geranylgeranyl pyrophosphate synthase and truncated 3-hydroxy-3-methyl glutaryl coenzyme A reductase,which rewired the GA3 biosynthesis *** results highlight the efficiency of our established system in recycling selection markers during iterative gene editing ***,the selection marker recycling system lays the foundation for further research on metabolic engineering for GA3 industrial production.

关键词： F.fujikuroi Gibberellic acid Plant growth hormone Selection marker recycling system CRISPR/Cas9 pyr4-blaster

Minocycline in the eradication of Helicobacter pylori infection: A systematic review and meta-analysis

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2024年第17期30卷 2354-2368页

作者： Kai Zhou cai-ling Li Hua Zhang Bao-Jun Suo Yu-Xin Zhang Xin-Lu Ren Yu-Xin Wang Chang-Min Mi ling-ling Ma Li-Ya Zhou Xue-Li Tian Zhi-Qiang Song Department of Gastroenterology Peking University Third HospitalBeijing 100191China

BACKGROUND Difficulty in obtaining tetracycline,increased adverse reactions,and relatively complicated medication methods have limited the clinical application of the classic bismuth quadruple ***,the search for new alternative drugs has become one of the research *** recent years,minocycline,as a semisynthetic tetracycline,has demonstrated good potential for eradicating Helicobacter pylori(***)infection,but the systematic evaluation of its role remains *** To explore the efficacy,safety,and compliance of minocycline in eradicating *** *** We comprehensively retrieved the electronic databases of PubMed,Embase,Web of Science,China National Knowledge Infrastructure,SinoMed,and Wanfang database as of October 30,2023,and finally included 22 research reports on *** eradication with minocycline-containing regimens as per the inclusion and exclusion *** eradication rates of *** were calculated using a fixed or a random effect model,and the heterogeneity and publication bias of the studies were *** The single-arm meta-analysis revealed that the minocycline-containing regimens achieved good overall *** eradication rates,reaching 82.3%[95%confidence interval(CI):79.7%-85.1%]in the intention-to-treat analysis and 90.0%(95%CI:87.7%-92.4%)in the per-protocol *** overall safety and compliance of the minocycline-containing regimens were good,demonstrating an overall incidence of adverse reactions of 36.5%(95%CI:31.5%-42.2%).Further by traditional meta-analysis,the results showed that the minocycline-containing regimens were not statistically different from other commonly used eradication regimens in eradication rate and incidence of adverse *** of the adverse reactions were mild to moderate and well-tolerated,and dizziness was relatively prominent in the minocycline-containing regimens(16%).CONCLUSION The minocycline-containing regimens demonstrated good efficacy,safety,and

关键词： Helicobacter pylori Minocycline Eradication Safety Resistance

Automatic detection of small bowel lesions with different bleeding risks based on deep learning models

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2024年第2期30卷 170-183页

作者： Rui-Ya Zhang Peng-Peng Qiang ling-Jun cai Tao Li Yan Qin Yu Zhang Yi-Qing Zhao Jun-Ping Wang Department of Gastroenterology The Fifth Clinical Medical College of Shanxi Medical UniversityTaiyuan 030012Shanxi ProvinceChina School of Computer and Information Technology Shanxi UniversityTaiyuan 030006Shanxi ProvinceChina School of Life Sciences and Technology Mudanjiang Normal UniversityMudanjiang 157011Heilongjiang ProvinceChina

BACKGROUND Deep learning provides an efficient automatic image recognition method for small bowel(SB)capsule endoscopy(CE)that can assist physicians in ***,the existing deep learning models present some unresolved *** To propose a novel and effective classification and detection model to automatically identify various SB lesions and their bleeding risks,and label the lesions accurately so as to enhance the diagnostic efficiency of physicians and the ability to identify high-risk bleeding *** The proposed model represents a two-stage method that combined image classification with object ***,we utilized the improved ResNet-50 classification model to classify endoscopic images into SB lesion images,normal SB mucosa images,and invalid ***,the improved YOLO-V5 detection model was utilized to detect the type of lesion and its risk of bleeding,and the location of the lesion was *** constructed training and testing sets and compared model-assisted reading with physician *** The accuracy of the model constructed in this study reached 98.96%,which was higher than the accuracy of other systems using only a single *** sensitivity,specificity,and accuracy of the model-assisted reading detection of all images were 99.17%,99.92%,and 99.86%,which were significantly higher than those of the endoscopists’*** image processing time of the model was 48 ms/image,and the image processing time of the physicians was 0.40±0.24 s/image(P<0.001).CONCLUSION The deep learning model of image classification combined with object detection exhibits a satisfactory diagnostic effect on a variety of SB lesions and their bleeding risks in CE images,which enhances the diagnostic efficiency of physicians and improves the ability of physicians to identify high-risk bleeding groups.

关键词： Artificial intelligence Deep learning Capsule endoscopy Image classification Object detection Bleeding risk

制造工艺对飞机铝锂合金结构细节疲劳性能影响研究

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

机械科学与技术 2024年第3期43卷 533-539页

作者：蔡玲上海飞机设计研究院上海201210

研究制造工艺(铣圆转速、孔边倒角、干涉量等)对飞机铝锂合金结构细节疲劳性能DFR的影响。结果表明:在一定铣圆转速范围内,存在最优铣圆转速;孔边倒角可以有效减轻应力集中现象,提高疲劳性能;干涉配合铆接在孔壁引入残余压应力,对孔连... 详细信息

研究制造工艺(铣圆转速、孔边倒角、干涉量等)对飞机铝锂合金结构细节疲劳性能DFR的影响。结果表明:在一定铣圆转速范围内,存在最优铣圆转速;孔边倒角可以有效减轻应力集中现象,提高疲劳性能;干涉配合铆接在孔壁引入残余压应力,对孔连接具有一定强化作用,通过增加干涉量可使连接达到与整体材料相当的疲劳寿命,不再是铆接件中最薄弱“环节”。

关键词：铝锂合金细节疲劳额定值铣切速度孔边倒角

维普期刊数据库

Association of preschool children behavior and emotional problems with the parenting behavior of both parents

在线全文

学校读者我要写书评

暂无评论

World Journal of Clinical Cases 2024年第6期12卷 1084-1093页

作者： Su-Mei Wang Shuang-Qin Yan Fang-Fang Xie Zhi-ling cai Guo-Peng Gao Ting-Ting Weng Fang-Biao Tao Department of Child Health Ma’anshan Maternal and Child Health CenterMa’anshan 243011Anhui ProvinceChina Department of Maternal Child and Adolescent HealthSchool of Public HealthAnhui Medical UniversityHefei 230032Anhui ProvinceChina

BACKGROUND Parental behaviors are key in shaping children’s psychological and behavioral development,crucial for early identification and prevention of mental health issues,reducing psychological trauma in *** To investigate the relationship between parenting behaviors and behavioral and emotional issues in preschool *** From October 2017 to May 2018,7 kindergartens in Ma’anshan City were selected to conduct a parent self-filled questionnaire-Health Development Survey of Preschool ***’s Strength and Difficulties Questionnaire(Parent Version)was applied to measures the children’s behavioral and emotional *** behavior was evaluated using the Parental Behavior *** logistic regression model was used to analyze the association between the detection rate of preschool children’s behavior and emotional problems and their parenting *** High level of parental support/participation was negatively correlated with conduct problems,abnormal hyperactivity,abnormal total difficulty scores and abnormal prosocial behavior *** level of maternal support/participation was negatively correlated with abnormal emotional symptoms and abnormal peer interaction in *** level of parental hostility/coercion was positively correlated with abnormal emotional symptoms,abnormal conduct problems,abnormal hyperactivity,abnormal peer interaction,and abnormal total difficulty scores in children(all P0.05),after calculating ratio of odds ratio *** Our study found that parenting behaviors are associated with behavioral and emotional issues in preschool ***,the more supportive or involved the parents are,the fewer behavioral and emotional problems the children experience;conversely,the more hostile or contro

关键词： Children Preschool age Parenting Behavioral Parenting problems

Microfluidic bubble-generator enables digital light processing 3D printing of porous structures

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Aggregate

Aggregate 2024年第1期5卷 280-291页

作者： Philipp Weber ling cai Francisco Javier Aguilar Rojas Carlos Ezio Garciamendez-Mijares Maria Celeste Tirelli Francesco Nalin Jakub Jaroszewicz Wojciech Swi eszkowski Marco Costantini Yu Shrike Zhang Division of Engineering in Medicine Department of MedicineBrigham and Women’s HospitalHarvard Medical SchoolCambridgeMassachusettsUSA Institute of Physical Chemistry—Polish Academy of Sciences KasprzakaPoland Biomaterials Group Materials Design DivisionFaculty of Materials Science and EngineeringWarsaw University of TechnologyWoloskaPoland

Three-dimensional(3D)printing is an emerging technique that has shown promising success in engineering human tissues in recent *** development of vatphotopolymerization printing modalities has significantly enhanced the complexity level for 3D printing of various functional structures and ***,the development of microfluidic chip systems is an emerging research sector with promising medical *** work demonstrates the coupling of a digital light processing(DLP)printing procedure with a microfluidic chip system to produce size-tunable,3D-printable porosities with narrow pore size distributions within a gelatin methacryloyl(GelMA)hydrogel *** is found that the generation of size-tunable gas bubbles trapped within an aqueous GelMA hydrogel-precursor can be controlled with high ***,the porosities are printed in two-dimensional(2D)as well as in 3D using the DLP *** addition,the cytocompatibility of the printed porous scaffolds is investigated using fibroblasts,where high cell viabilities as well as cell proliferation,spreading,and migration are *** is anticipated that the strategy is widely applicable in a range of application areas such as tissue engineering and regenerative medicine,among others.

关键词： 3D printing biofabrication bubble digital light processing microfluidics porous

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

考虑差异化需求的电动汽车充电调度策略

控制与决策 2024年第3期39卷 795-803页

作者：蔡凌郭戈施冷安东东北大学秦皇岛分校控制工程学院河北秦皇岛066004

电动汽车行驶里程短、充电时间长是影响驾驶体验的关键.通过对电网、充电设备进行大规模升级的方法减少充电时间,成本昂贵,因此充分利用现有路网、电网资源,制定智能充电调度策略成为提高驾驶体验的重要手段.考虑到驾驶者对充电时间敏... 详细信息

电动汽车行驶里程短、充电时间长是影响驾驶体验的关键.通过对电网、充电设备进行大规模升级的方法减少充电时间,成本昂贵,因此充分利用现有路网、电网资源,制定智能充电调度策略成为提高驾驶体验的重要手段.考虑到驾驶者对充电时间敏感度的异质性,提出具有差异化的调度策略以满足不同优先级驾驶者的需求.首先,为均衡不同优先级驾驶者的利益,提出一种基于动态截断机制的两优先级队列模型;其次,定义充电站的准入原则,保证高优先级驾驶者对预留桩的使用权及对空闲桩的优先抢占权;然后,提出基于截断机制的双层优化模型CCPQ(charging with cut-off priority queue),在顶层高优先级车辆与充电桩最优匹配的基础上,设计底层低优先级车辆的分配策略优化模型,将最小化低优先级驾驶者的总等待时间构建为凸优化问题;最后,通过仿真验证策略的有效性及优越性.

关键词：电动汽车充电策略区分服务预约截断优先级队列凸优化

维普期刊数据库

Pulmonary abscess caused by Streptococcus pseudopneumoniae in a child:A case report and review of literature

在线全文

学校读者我要写书评

暂无评论

World Journal of Radiology 2024年第8期16卷 362-370页

作者： Ran Ma Yan-Mei Wang Hua Guan Li Zhang Wei Zhang ling-cai Chen Department of Pediatrics Shihezi UniversityShihezi 832000Xinjiang Uygur Autonomous RegionChina Department of Pediatrics The First Affiliated Hospital of Shihezi UniversityShihezi 832000Xinjiang Uygur Autonomous RegionChina Department of Pediatrics Corps Fourth Division HospitalYining 844500Xinjiang Uygur Autonomous RegionChina Department of Pediatrics Corps First Division HospitalAksu 842008Xinjiang Uygur Autonomous RegionChina

BACKGROUND Lung abscess found on chest X-ray and computed tomography examinations is rare in infants and young *** pathogens can cause lung abscesses,with the most common pathogens being anaerobes,Streptococci and Staphylococcus *** pseudopneumoniae(***)is a member of the Streptococcaceae family,and is mainly isolated from respiratory tract *** are currently no cases of lung abscess caused by *** in the *** SUMMARY A 2-year-old boy was admitted to hospital due to persistent cough and *** computed tomography examination suggested the formation of a lung *** diagnosis was not confirmed by testing for serum respiratory pathogens(6 items),respiratory pathogen nucleic acid(27 items),and laboratory ***,metagenomic next-generation sequencing of bronchoalveolar lavage fluid revealed the presence of ***,confirming its role in causing the lung *** receiving antibiotic treatment,reexamination with lung computed tomography showed that the abscess was resorbed and the patient’s outcome was *** This is the first report of a lung abscess in a child caused by *** *** next-generation sequencing of bronchoalveolar lavage fluid is helpful in achieving rapid and accurate pathogen identification.

关键词： Streptococcus pseudopneumoniae Lung abscess Children Bronchoalveolar lavage fluid Metagenomic nextgeneration sequencing Case report

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

职业院校校本数据治理的转型逻辑与实践范式

教育与职业 2024年第2期1050卷 43-51页

作者：黄茂勇蔡凌广东技术师范大学教育科学学院广东广州510665

职业院校数据治理是职业教育治理体系现代化的核心要素。职业院校校本数据治理范式的提出,符合国家职业教育治理体系从宏观建构转向微观规范的政策诉求。职业院校校本数据治理遵循从碎片转向全局的价值逻辑、从孤岛转向共生的过程逻辑... 详细信息

职业院校数据治理是职业教育治理体系现代化的核心要素。职业院校校本数据治理范式的提出,符合国家职业教育治理体系从宏观建构转向微观规范的政策诉求。职业院校校本数据治理遵循从碎片转向全局的价值逻辑、从孤岛转向共生的过程逻辑、由浅表转向深度的技术逻辑及从适应转向预测的功能逻辑等四重转型逻辑。在实践场域,职业院校校本数据治理存在数据生态失衡与数据架构断点、数据规格泛化与信息离散、数据治理政策缺位与流程迟滞、数据治理偏利取向与效用失准等现实问题。推动职业院校校本数据治理实践范式建构,可从基于国家“智慧大脑”架构的共生共治价值体系建构、基于数据生命周期的数据全链条管理过程机制建构、基于多源异构数据的分层治理技术载体建制、基于师生同频成长支持体系动力设计的功能系统调试四个维度展开。

关键词：职业院校数据治理整体性治理治理体系现代化

维普期刊数据库