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Glutamicibacter nicotianae AT6:A new strain for the efficient biodegradation of tilmicosin

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Journal of Environmental Sciences 2024年第8期142卷 182-192页

作者： Huijuan Li hao Zhou liling Fan Long Meng Yanyun Zhao Lanmei Zhao Bo Wang College of Chemical and Biological Engineering Shandong University of Science and TechnologyQingdao 266590China College of Chemistry and Chemical Engineering Heze UniversityHeze 274015China College of Safety and Environmental Engineering Shandong University of Science and TechnologyQingdao 266590China

The degradation of tilmicosin(TLM),a semi-synthetic 16-membered macrolide antibiotic,has been receiving increasing ***,there are three tilmicosin degradation methods,and among them microbial degradation is considered the best due to its high efficiency,eco-friendliness,and low ***,we found a new strain,Glutamicibacter nicotianae ***6,capable of degrading high-concentration TLM at 100 mg/L with a 97%removal *** role of tryptone was as well investigated,and the results revealed that the loading of tryptone had a significant influence on TLM *** toxicity assessment indicated that strain AT6 could efficiently convert TLM into less-toxic *** on the identified intermediates,the degradation of TLM by AT6 processing through two distinct pathways was then proposed.

关键词： Tilmicosin Glutamicibacter nicotianae Biodegradation Degradation pathway Pharmaceutical wastewater

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铜复合金属材料抗菌特性试验研究

工业微生物 2024年第4期54卷 75-81页

作者：于劲松黄煦任方圆仲歆怡曹慧袁敏叶泰吴秀秀郝丽玲徐斐上海理工大学健康科学与工程学院上海200093 上海食品快速检测工程技术研究中心上海200093

文章将大肠埃希氏菌作为试验菌种,采用平板计数法评价铜及铜复合金属材料的抗菌性能。试验中,将适宜稀释度的菌悬液分别滴加在空白样本(聚乙烯片)和实验样本(白铜片、磷铜片和铍青铜片)上接触培养一段时间,洗脱后将洗脱液涂布于平板上... 详细信息

文章将大肠埃希氏菌作为试验菌种,采用平板计数法评价铜及铜复合金属材料的抗菌性能。试验中,将适宜稀释度的菌悬液分别滴加在空白样本(聚乙烯片)和实验样本(白铜片、磷铜片和铍青铜片)上接触培养一段时间,洗脱后将洗脱液涂布于平板上进行培养,并进行菌落计数,以研究铜合金材料的抗菌特性,探索铜合金抗菌材料在诸多领域的深入应用。结果表明,接触白铜、磷铜、铍青铜3种材料24 h,稀释105倍的菌液培养24 h后基本无菌落生长。试验结果显示,铜合金材料的平均抗菌率R逸99%,且材料中起主要抗菌作用的成分是铜。铜合金材料的抗菌率与纯铜材料的抗菌率相近,且结果均符合玉级抗细菌率标准,所以判断这三类铜合金材料均具有强抗菌效果。

关键词：铜复合材料抗菌性大肠埃希氏菌

Effects of Simulated Grazing Pattern and Nitrogen Supply on Plant Growth in a Semiarid Region of Northern China

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Acta Botanica Sinica 2004年第9期46卷 1032-1039页

作者： YUANZhi-You liling-hao HANXing-Guo JIANGFeng-He LINGuo-Hui ZhaoMing-Xu RENLi-Yun LaboratoryofQuantitativeVegetationEcology InstituteofBotanyTheChineseAcademyofSciencesBeijing100093China GrasslandManagementStationofDuolunCountyDuolun027300 NeiMongolChina AnimalHusbandryBureauofTaipusi Taipusi027000NeiMongolChina

Grazing in grassland ecosystems affects plant growth by removing biomass and depositing excretal nutrients. However, grazing is not uniformly distributed in space. The spatial pattern of defoliation and excretion deposition by herbivores across vegetation mosaics has been frequently discussed, but rarely spatially quantified. A 60-day field experiment in a native semiarid grassland community was conducted to examine the responses of plant growth to simulated grazing pattern and varying nitrogen *** were subjected to five defoliation treatments determined by circularly clipped patches of different size (0, 10, 20, 40, 80cm in radius), and four nitrogen supply levels in soils (0, 5, 10, 20g N/m^2). It was detected that defoliation had reduced primary productivity by 41.5% whereas fertilization had increased it by 57.8%. The negative effect of defoliation was greater in the smallest, fertilized patches. N addition had been found to have altered the effect of defoliation, as plants growing at higher nitrogen levels were more negatively affected by defoliation than plants with no supplementary application of nitrogen. These results indicated that the magnitude of defoliation response for an individual plant was modulated by not only defoliation itself, but also other factors, such as nutrient availability, The increase in the ratio of live to dead plant parts suggested that urine deposition delayed the senescence of plants. The results also showed that (1) the effect of defoliation on primary productivity was affected by the patch size, and (2) nitrogen addition (simulated urine deposition) could increase primary productivity and affect the response to defoliation more obviously in the smaller patches than in the larger ones.

关键词：氮半干旱地区中国北方地区放牧牧场施肥植物生长

Effects of Temperature and Several Chemicals on Metabolic Changes During Dormancy Release in NJ72 Nectarine

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Agricultural Sciences in China 2003年第5期2卷 549-555页

作者： YUANZhi-you LIXian-li liling-hao HANXing-guo YANTian-li LaboratoryofQuantitativeVegetationEcology InstituteofBotanyTheChineseAcademyofSciencesBeijing100093 HorticulturalCollegeofShandongAgriculturalUniversity Tai‘an271018P.R.China

Poor, delayed and ununiform budbreak is a major problem for peaches in greenhouse. To clarify the mechanism of breaking bud dormancy in nectarines, the effect of temperature and three dormancy-breaking agents on metabolic changes during dormancy release in two-year old NJ72 nectarine (Prunus persica L. Batch) was investigated. The result showed temperature and chemicals affected the budbreak and the metabolism of NJ72 nectarine during dormancy. Endogeneous peroxide content in buds increased soon after low temperature treatment. Meanwhile, catalase activity was also shown to increase significantly at low temperature treatment, coincided with increase of the activity of peroxidase and superoxide dismutase. The rate of respiration in flower buds increased at low temperature during dormancy. The rate of the pentose phosphate pathway increased, while the rate of the Embden-Meyerhof pathway decreased and the rate of tricarboxlic acid cycle changed little. Glucose 6-phosphate dehydrogenase activity increased at low temperature during dormancy. At the same time we found an accumulation of peroxide after treatment with dormancy-breaking chemicals. In flower buds treated with dormancy-breaking agents, thiourea, KNO3 and NH4NO3, catalase activity was inhibited soon after treatment, whereas peroxidase activity increased, and the changes of superoxide dismutase remained little. In this study, it was found that the rates of respiration in flower buds increased by chemicals sprays during dormancy. The activity of glucose 6-phosphate dehydrogenase, the key enzyme in the pentose phosphate pathway (PPP), increased by spraying with dormancy-breaking agents, concomitantly with the activation of the pentose phosphate pathway.

关键词： Prunus persica L. Batch Dormancy Temperature Chemicals

Conceptual Analysis of Self-Management in Patients with Hypertension

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Journal of Clinical and Nursing Research 2024年第5期8卷 356-368页

作者： Li Yang Yutian hao liling Zhong Shilin Xu Saiqiong Zhong Yanying Lu Hui Liu The Fifth Affiliated Hospital Southern Medical UniversityGuangzhouGuangdong ProvinceChina Shenzhen Children’s Hospital ShenzhenGuangdong ProvinceChina The Guangzhou Red Cross Hospital GuangzhouGuangdong ProvinceChina The Second Affiliated Hospital of Shantou University Medical College ShantouGuangdong ProvinceChina

Objective:To clarify the concept of self-management in hypertensive patients by analyzing the definition,attributes,and measurement tools through a literature ***:An Internet-based search of the databases was conducted using Academic Search Complete,Medical Line,CINAHL,Health Source:Nursing/Scholarly Edition,and Google *** the search process,keywords or free text were combined by using Boolean operators,with the search terms“self of management”or“self-management,”“concept*analysis”or“concept*definition,”and Walker and Avant’s concept analysis method was ***:Analysis of relevant literature summarized the conceptual attributes of self-management in hypertensive patients as the active participation of patients in the treatment process;the presence of interaction provided by patients and health care providers;the use of certain health management tools;and the aim of maintaining and improving the health status and living capacity of hypertensive ***:The concept of self-management for hypertensive patients was clarified,which helps to promote the application of hypertensive self-management in clinical work and improve outcomes and quality of life for patients with hypertension.

关键词： Hypertension Self-management Concept analysis Walker and Avant’s concept analysis method

我国公共体育服务多元供给的协同创新模式及推进路径

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上海体育学院学报 2017年第6期41卷 48-52,59页

作者：郝利玲安徽师范大学体育学院安徽芜湖241000

我国公共体育服务供给中存在政府失灵、市场失灵和社会失灵问题。目前的多元合作供给仅是一种表象合作,并未形成真正合力。提出:以协同创新理论为指导,通过多元供给主体战略之间互相包容、推动,服务资源之间互相补充、利用,组织要素之... 详细信息

我国公共体育服务供给中存在政府失灵、市场失灵和社会失灵问题。目前的多元合作供给仅是一种表象合作,并未形成真正合力。提出:以协同创新理论为指导,通过多元供给主体战略之间互相包容、推动,服务资源之间互相补充、利用,组织要素之间互相信任、理解,服务环境之间的互惠互利、互相支持,促进我国公共体育服务的有效供给。

关键词：公共体育服务多元供给协同创新模式路径

Plateau Marsh Methane Oxidation as affected by Inorganic N

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Pedosphere 2004年第2期14卷 195-204页

作者： WANGZhi-Ping DUANYi YANGJu-Rong liling-hao HANXing-Guo LaboratoryofQuantitativeVegetationEcology InstituteofBotanyChineseAcademyofSciencesBeijing100093（China）／E-mail：wangzp5@*** StateKeyLaboratoryofGasGeochemistry LanzhouInstituteofGeology.ChineseAcademyofSciencesLanzhou730000（China） CollegeofResourceandEnvironment BEijingNormailUniversityBeijing100875（China）

In a series of laboratory incubations using soils of two contrasting sites from a temperate marsh on the QinghaiTibet Plateau,potential methane (CH4) oxidation rates were measured to study the effects of inorganic N inputs on CH4 oxidation. For a drained site,subsurface peat (5-15 cm) at an initial 20 μL CH4 L-1 showed a significantly different (P＜0.05) CH4 oxidation rate compared to other soil depths,with a maximal rate of 20.9 ng CH4 gDW (dry weight)-1h-1; the underlying mineral soil layers (15-30 and 30-50 cm) also had a strong CH4 oxidation capacity at about an initial 2000 μL CH4 L-1. With a waterlogged site,the CH4 oxidation rate in an aerobic incubation was significantly greater (P＜0.05) in the surface soil layer (0-5cm) compared to the 15-30 and 30-50 cm depths. There was generally no or a very weak effect from addition of NO3- on CH4 oxidation. In marked contrast,NH4+ salts,such as (NH4)2SO4,NH4C1 and NH4NO3,exhibited strong inhibitions,which varied as a function of the added salts and the initial CH4 level. Increasing NH4+ usually resulted in greater inhibition and increasing initial CH4 concentrations resulted in less. NH4+ inhibition on CH4 oxidation in natural high-altitude,low-latitude wetlands could be as important as has been reported for agricultural and forest soils. The NH4+ effects on the CH4 oxidation rate need to be further investigated in a wide range of natural wetland soil types.

关键词：沼气湿地氧化无机物氮

工程教育认证背景下“食品化学”教学改革探索

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食品工业 2022年第11期43卷 184-186页

作者：郝丽玲叶泰徐斐上海理工大学健康科学与工程学院上海200093 上海食品快速检测工程中心上海200093

“食品化学”是食品类专业核心课程之一,着重学生发现问题、分析问题及解决问题能力的培养。在工程教育认证背景下,以教师讲授为主的传统“食品化学”教学存在学生学习积极性不佳、学生解决复杂工程问题能力未被有效培养的问题,无法达... 详细信息

“食品化学”是食品类专业核心课程之一,着重学生发现问题、分析问题及解决问题能力的培养。在工程教育认证背景下,以教师讲授为主的传统“食品化学”教学存在学生学习积极性不佳、学生解决复杂工程问题能力未被有效培养的问题,无法达到食品科学与工程专业人才培养目标,无法满足食品行业人才需求。从3个方面(树立典型教学案例,调动学习积极性,增强专业认同感和行业归属感;强化食品化学学科的逻辑线条梳理,培养学生分析和解决问题的能力;丰富教学形式,完善考核方式,提升学生综合素养)对“食品化学”教学体系进行教学改革研究探索。以有力支撑培养“具有国际化视野及终身学习能力的卓越工程技术型食品行业人才”的育人目标,满足新时代背景下食品行业的人才需求。

关键词：工程教育认证食品化学教学改革

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基于环形电极的开放式MREIT三维重建

仪器仪表学报 2012年第10期33卷 2263-2269页

作者：李刚陈瑞娟郝丽玲林凌天津大学精密测试技术及仪器国家重点实验室天津300072

针对主磁场为竖直方向的开放式磁共振系统,提出一种环形电极模式应用于三维磁共振电阻抗成像(magnetic resonance electrical impedance tomography,MREIT)。首先通过对开放式磁共振系统中不同电流注入模式的分析,提出了基于环形电极的... 详细信息

针对主磁场为竖直方向的开放式磁共振系统,提出一种环形电极模式应用于三维磁共振电阻抗成像(magnetic resonance electrical impedance tomography,MREIT)。首先通过对开放式磁共振系统中不同电流注入模式的分析,提出了基于环形电极的电流注入模式;然后研究了基于广义最小余量方法的单层灵敏度矩阵算法,将其用于三维电导率图像重建;最后用模拟数据验证了所提出的电流模式和所发展的算法。基于三维圆柱模型和真实小腿模型的仿真实验表明:环形电极模式适用于开放式磁共振系统的MREIT磁场测量,仅注入一支电流,并利用单一磁通密度分量即可实现重构,且电导率重建图像具有较高的定位准确性和抗噪性能;基于真实小腿模型的仿真实验初步验证了该方法用于复杂人体电导率检测的可行性。环形电极模式的提出对于开放式MREIT的广泛使用具有潜在的应用价值。

关键词：开放式磁共振成像磁共振电阻抗成像环形电极三维重建