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The comparison of biomarkers released by hydropyrolysis and Soxhlet extraction from source rocks of different maturities

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Chinese Science Bulletin 2013年第3期58卷 373-383页

作者： WU LiangLiang liao YuHong FANG YunXin GENG AnSong State Key Laboratory of Organic Geochemistry Guangzhou Institute of GeochemistryChinese Academy of Sciences Graduate University of Chinese Academy of Sciences

The Permian Dalong Formation(P2d) source rocks from the mature Guangyuan outcrop section and the overmature Wangcang outcrop section in Sichuan Basin were *** Soxhlet extraction and kerogen catalytic HyPy were conducted on the P2d source *** biomarkers obtained by both methods were compared to discuss their difference and the influence extent of thermal maturation on covalently bound *** results show that covalently bound biomarkers can hardly be correlated to the corresponding free biomarkers due to severe thermal alterations and/or interference of migrated hydrocarbons in both outcrop sections and thermal maturation have much lower influence on covalently bound biomarkers in kerogens than on free biomarkers in Soxhlet *** application of HyPy in study of biomarker geochemistry can reduce the thermal maturation effect on biomarkers(Ro≤2.4%) to a greater extent and the interference of migrated hydrocarbons between interbedded *** covalently bound biomarkers released by HyPy are useful in the study of biomarker geochemistry and oil-source correlation on high-overmature source rocks.

关键词：生物标志物加氢热解索氏提取烃源岩共价键结合地球化学特征热成熟度四川盆地

Establishment of an avian leukosis virus subgroup A-resistant cell line

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Journal of Integrative Agriculture 2017年第4期16卷 930-936页

作者： FENG Min DAI Man-man liao Ming CAO Wei-sheng College of Veterinary Medicine South China Agricultural University Guangzhou 510642 P.R.China National and Regional Joint Engineering Laboratory for Medicament of Zoonosis Prevention and Control Guangzhou 510642 P.R.China Key Laboratory of Veterinary Vaccine Innovation Ministry of Agriculture Guangzhou 510642 P.R.China Key Laboratory of Zoonosis Prevention and Control of Guangdong Province Guangzhou 510642 P.R. China

Rapid diagnostic methods for classifying avian leukosis subgroups in the field were needed for routine, large-scale screening. As a first step in method development, we inserted the avian leukosis virus subgroup A （ALV-A） env gene into plasmid pcDNA3.1/Zeo （＋） and used this construct to transfect DF-1 cells. Zeocin-resistant cells were obtained after 2 weeks of zeocin selection. Then, the cells were analyzed using PCR, immunofluorescence, and Western blot for expression of the envA-encoded envelope protein after 30 serial passages. The DF-1/A cell line was completely resistant to 104 TCIDso/0.1 mL （50% tissue culture infective dose）ALV-A and was partially resistant to 10~ TCIDs0/0.1 mL ALV-A viral particles. By comparing the DF-1/A and DF-1 cell lines, an ALV-A isolate was identified using a gag-specific ELISAfor capsid protein p27. Thus, we established a DF-1/A cell line that was resistant to ALV-A infection. This cell line will be useful as a diagnostic tool.

关键词： avian leukosis virus subgroup A (ALV-A) env DF-1/A cell line diagnostic tool

A new and efficient method for the synthesis of isoquinoline-3-carboxylate

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Chinese Chemical Letters 2008年第3期19卷 253-255页

作者： Xiang Wei liao Bao He Guan Zhan Zhu Liu Institute of Materia Medica Chinese Academy of Medical Sciences ＆ Peking Union Medical College Beijing 100050. PR China

An isoquinoline-3-carboxylate compound 3 was obtained with a moderate yield of 40% when N-acetyl-（3′-hydroxy-4′- methoxy-5′-methyl）phenylalanine methyl ester 1 was refluxed in HMTA/TFA. However, the anticipated product N-acetyl-（3′- hydroxy-4′-methoxy-5′-methyl-6′-formyl）phenylalanine methyl ester 2 could not be found. The possible mechanism was discussed in this article.

关键词： Isoquinolinc-3-carboxylate Duff reaction N-Acetyl-phenylalanine methyl ester

Reconsideration of the sediment characteristics of the second member of the Triassic Xujiahe Formation in Sanhuiba,Huaying Mountain

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Mining Science and Technology 2010年第4期20卷 591-599页

作者： liao Taiping HU Jingjing ZHANG Furong LIU Lijing Chongqing University of Science and Technology Chongqing 401331 China Research Institute of Petroleum Exploration and Development PetroChina Beijing 100083 China Institute of Geology and Geophysics Chinese Academy of Sciences Beijing 100029 China

The second member of the Triassic Xujiahe Formation in the Sanhuiba area in Huaying Mountain is an important section of sedimentary delta facies in the Chongqing Tianfu Geological Survey and Training Base. In spite of some initial efforts on its lithology and lithofacies, the existing research is yet to be completed and suffers from contradictory conclusions and inadequate evidence. Starting from the sedimentary microfaeies types and cyclic features, we analyzed the vertical sequence of a sedimentary delta model and discovered that this section is a constructional delta deposit. Eight incomplete and asymmetric regressive cycles are marked off, given that frequent erosion surfaces appeared in the section. Based on the analysis of structural characteristics of every monocycle, we summarized the system of cycles, tracts and sequence characteristics of the overall section. In view of this systematic series of cycles, we discussed the features of the syndepositional tectonic movements in the area and conclude that, due to frequent erosion, migration and diversion of a sub-channel branch was brought about by oscillating movements of the crust. In a comparison with neighboring sections, we explored the cause of erosion and the relations between erosion and cycles. This study greatly strengthens existing research and theories, makes an important and comprehensive contribution to geological surveys and training in the area and provides essential suggestions for coal exploration, oil and gas evaluation and in general to exploration and development in the target stratum.

关键词： Huaying Mountain Sanhuiba Xujiahe Formation member 2 sandbodies sedimentary characteristics reconsideration

Detection of leaf structures in close-range hyperspectral images using morphological fusion

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Geo-Spatial Information Science 2017年第4期20卷 325-332页

作者： Gladys Villegas Wenzhi liao Ronald Criollo Wilfried Philips Daniel Ochoa Department of Telecommunications and Information Processing Ghent University-imec Ghent Belgium Facultad de Ingeniería en Eléctrica y Computación ESPOL Polytechnic University Escuela Superior Politécnica del Litoral Guayaquil Ecuador

Close-range hyperspectral images are a promising source of information in plant biology,in particular,for in vivo study of physiological *** this study,we investigate how data fusion can improve the detection of leaf elements by combining pixel reflectance and morphological *** detection of image regions associated to the leaf structures is the first step toward quantitative analysis on the physical effects that genetic manipulation,disease infections,and environmental conditions have in *** tested our fusion approach on Musa acuminata (banana) leaf images and compared its discriminant capability to similar techniques used in remote *** results demonstrate the efficiency of our fusion approach,with significant improvements over some conventional methods.

关键词： Hyperspectral fusion morphology plant biology

北部湾城市群经济协同发展研究——基于经济联系视角

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国土资源科技管理 2024年第4期41卷 85-97页

作者：占金刚杨晓彦廖贵雲北部湾大学经济管理学院广西钦州535011

文章以北部湾城市群的全部地级市、县域为研究对象,运用修正引力模型,测度2008—2020年北部湾城市群15个市、县之间的经济联系强度,并分析北部湾城市群的城市间联系强度、省域间经济联系强度、经济联系总强度。研究发现:(1)北部湾城市... 详细信息

文章以北部湾城市群的全部地级市、县域为研究对象,运用修正引力模型,测度2008—2020年北部湾城市群15个市、县之间的经济联系强度,并分析北部湾城市群的城市间联系强度、省域间经济联系强度、经济联系总强度。研究发现:(1)北部湾城市群各城市之间的经济联系强度值呈逐年上升的趋势,且较弱联系强度及以上的城市组合数大致都在增加,极弱联系强度的城市组合数则在减少,但北部湾城市群各城市间的经济联系强度仍处于相对弱的阶段。(2)北部湾城市群中同省域的城市经济联系相对紧密,省域之间的城市经济联系相对松散,且海南省的城市尤其是“三县”的内外部经济联系强度亟待提高。(3)北部湾城市群的经济联系强度总值呈近似线性增长趋势,但北部湾城市群各城市的经济联系发展不平衡,经济联系强度分层明显,且北部湾城市群发展的“一湾双轴、一核两极”的城市群框架并未形成,“一湾”经济发展活力不足,“双轴”已初具规模,“一核”有待加强建设,“两极”正逐渐增强集聚作用。由此,文章提出相对应的优化建议,以促进北部湾城市群的经济协同发展。

关键词：北部湾城市群协同发展经济联系修正引力模型

基于创造性思维方法的景观设计在线教学研究与实践

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华中建筑 2024年第7期42卷 148-151页

作者：廖启鹏闫玥霖王哲李汶钠中国地质大学(武汉)公共管理学院中国地质大学(武汉)艺术与传媒学院重庆大学建筑与城市规划学院

设计类在线教育由粗放式向精细化方向转变,创造性思维方式培养失位的问题日益突显。将传统的单向传输式的方法转变为增强师生交互协作性,锻造“创造性”设计能力的在线教学方法是必然趋势。基于上述分析,以在线教学为实证,提出从思维培... 详细信息

设计类在线教育由粗放式向精细化方向转变,创造性思维方式培养失位的问题日益突显。将传统的单向传输式的方法转变为增强师生交互协作性,锻造“创造性”设计能力的在线教学方法是必然趋势。基于上述分析,以在线教学为实证,提出从思维培养、语义转换到创作设计的教学方法。表明在线模式或混合模式是设计教育的发展趋势;创造性思维模式的培养是设计教育的内核;新技术的发展能够破解当前在线教育的困境,促进设计类在线教育的繁荣发展。

关键词：创造性在线教学语义转译

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后角形式对钻头性能的影响

现代机械 2024年第4期 27-30页

作者：廖湘辉尹麒麟汪万勇三峡大学机械与动力学院湖北宜昌443000 宜昌永鑫精工科技股份有限公司湖北宜都443000

后角形式对钻头的切削力、应力、温度等有着重要的影响。对钻头整个的切削过程建立三维模型,并进行热力耦合仿真,分析了不同后角形式的钻头在干切状态下的Mises应力、切削力及温度的变化状况,找出两者之间的优缺点。建立内冷状态下钻头... 详细信息

后角形式对钻头的切削力、应力、温度等有着重要的影响。对钻头整个的切削过程建立三维模型,并进行热力耦合仿真,分析了不同后角形式的钻头在干切状态下的Mises应力、切削力及温度的变化状况,找出两者之间的优缺点。建立内冷状态下钻头和冷却液流入流出三维模型,并进行热流耦合仿真,分析了不同后角形式的钻头冷却液流动及温度变化情况,找出两者之间的差距。为钻头的设计思路和应用场景提供参考。

关键词：钻头有限元仿真后角热力学流体力学

QUANTITATIVE MUELLER MATRIX POLARIMETRY TECHNIQUES FOR BIOLOGICAL TISSUES

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Journal of Innovative Optical Health Sciences 2012年第3期5卷 34-40页

作者： HONGHUI HE NAN ZENG DONGZHI LI RAN liao HUI MA Shenzhen Key Laboratory for Minimal Invasive Medical Technologies Shenzhen 518055 P.R.China Laboratory of Optical Imaging and Sensing Graduate School at Shenzhen Tsinghua UniversityShenzhen 518055P.R.China Department of Physics Tsinghua University Beijing 100084P.R.China

We propose and conduct both the rotating linear polarization imaging(RLPI)and Mueller matrix transformation(MMT)measurements of different biological tissue samples,and testify the capability of the Mueller matrix polarimetry for the anisotropic scattering *** independent parameters extracted from the RLPI and MMT techniques are compared and *** tissue experimental results show that the parameters are closely related to the structural characteristics of the turbid scattering media,including the sizes of the scatterers,the angular distribution and order of alignment of *** results and conclusions in this paper may provide a potential method for the detection of precancerous and early stage cancerous ***,such studies represent the Mueller matrix transformation procedure which results in a set of parameters linking up the Mueller matrix elements to the structural and optical properties of the media.

关键词： Polarization scattering Mueller matrix biological tissues