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Cellular Tolerance, Accumulation and Distribution of Cadmium in Leaves of Hyperaccumulator Picris divaricata

Pedosphere 2012年第4期22卷 497-507页

作者： HU Peng-Jie GAN Yuan-Yuan TANG Ye-tao ZHANG Quan-Fang JIANG Dan YAO Nan QIU Rong-liang School of Environmental Science and Engineering Sun Yat-sen UniversityGuangzhou 510275(China) Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology Guangzhou 510275(China) School of Life Sciences Sun Yat-sen UniversityGuangzhou 510275(China) Instrumental Analysis and Research Center Sun Yat-sen UniversityGuangzhou 510275(China)

Knowledge of cellular metal homeostasis will provide a better understanding of the mechanisms involved in metal tolerance and hyperaccumulation in metal-hyperaccumulating plants. Energy dispersive X-ray spectrometry （EDS） was used to determine the localization of cadmium （Cd） in leaves of the Zn/Cd hyperaccumulator Picris divaricata which had a shoot Cd concentration of 565 mg kg-1 after 2 weeks of growth in solution culture supplying 10μ tmol L^-1 CdCl2. The results indicated that Cd was distributed mainly in the trichomes, upper and lower epidermis and bundle sheath cells, with a relatively low level of Cd in mesophyll cells. Mesophyll protoplasts isolated from leaves remained viable after 24 h exposure to CdCl2 at a concentration up to 1 mmol L^-1, indicating their high tolerance to Cd. The intracellular Cd was visualized by staining with Leadmium Green dye, a cellular permeable Cd fluorescence probe. The results showed that the majority of protoplasts （〉 82%） did not accumulate Cd, with only a minority （〈 18%） showing Cd accumulation. In the Cd-accumulating protoplasts, Cd accumulation was depressed by the addition of Fe^2＋, Mn^2＋ and the metabolic inhibitor carbonyl cyanide m-chlorophenylhydrazone （CCCP）, but not by Ca^2＋ or Zn^2＋. Furthermore, the entire process of Cd uptake from external solution into the cytoplasm and subsequent sequestration into vacuoles was successfully recorded by confocal images. These results suggested that reduced cellular Cd accumulation and efficient Cd vacuolar sequestration in mesophyll cells might be responsible for cellular Cd tolerance and distribution in the leaves of P. divaricata.

关键词： confocal images fluorescence probe mesophyll protoplast vacuolar

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Mesothelin promotes cell proliferation in the remodeling of neonatal rat pancreas

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World Journal of Gastroenterology 2014年第22期20卷 6884-6896页

作者： Dan-Dan Yin liang-Hui You Qing-Xin Yuan Xiao-Di liang Ning Wang Lin-tao Wang Li Yuan Ke-Ming Wang Wei De Department of Central Laboratory the Second Affiliated Hospital of Southeast University 2th Department of Biochemistry and Molecular Biology Nanjing Medical University Department of Biochemistry and Molecular Biology Nanjing Medical University Department of Endocrinology First Affiliated Hospital of Nanjing Medical University Department of Oncology Second Affiliated Hospital of Nanjing Medical University

AIM: To investigate the effect of mesothelin in the remodeling of the endocrine pancreas in neonatal rats.

关键词： Mesothelin Pancreatic remodeling Cell proliferation Pancreatic development Small islets

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Characterization of acute-on-chronic liver diseases: A multicenter prospective cohort study

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World Journal of Hepatology 2024年第5期16卷 809-821页

作者： Yuan-Yao Zhang Sen Luo Hai Li Shu-Ning Sun Xian-Bo Wang Xin Zheng Yan Huang Bei-Ling Li Yan-Hang Gao Zhi-Ping Qian Feng Liu Xiao-Bo Lu Jun-Ping Liu Hao-Tang Ren Yu-Bao Zheng Hua-Dong Yan Guo-Hong Deng liang Qiao Yan Zhang Wen-Yi Gu Xiao-Mei Xiang Yi Zhou Yi-Xin Hou Qun Zhang Yan Xiong Cong-Cong Zou Jun Chen Ze-Bing Huang Xiu-Hua Jiang Ting-Ting Qi Yuan-Yuan Chen Na Gao Chun-Yan Liu Wei Yuan Xue Mei Jing Li tao Li Rong-Jiong Zheng Xin-Yi Zhou Jun Zhao Zhong-Ji Meng Department of Infectious Disease Institute of Biomedical ResearchRegulatory Mechanism and Targeted Therapy for Liver Cancer Shiyan Key LaboratoryHubei provincial Clinical Research Center for Precise Diagnosis and Treatment of Liver CancerTaihe HospitalHubei University of MedicineShiyan 442000Hubei ProvinceChina Department of Gastroenterology Renji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200120China Department of Infectious Diseases Southwest HospitalThe Third Military Medical University(Army Medical University)Chongqing 400000China Center of Integrative Medicine Beijing Ditan HospitalCapital Medical UniversityBeijing 100020China Department of Infectious Diseases Institute of Infection and ImmunologyUnion HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430020Hubei ProvinceChina Department of Infectious Diseases Hunan Key Laboratory of Viral HepatitisXiangya HospitalCentral South UniversityChangsha 110051Hunan ProvinceChina Hepatology Unit Department of Infectious DiseasesNanfang HospitalSouthern Medical UniversityGuangzhou 510515Guangdong ProvinceChina Department of Hepatology The First Hospital of Jilin UniversityChangchun 130021Jilin ProvinceChina Department of Liver Intensive Care Unit Shanghai Public Health Clinical CentreFudan UniversityShanghai 200093China Tianjin Institute of Hepatology Nankai University Second People's HospitalTianjin 300192China Infectious Disease Center The First Affiliated Hospital of Xinjiang Medical UniversityUrumqi 830054Xinjiang Uygur Autonomous RegionChina Department of Infectious Diseases and Hepatology The Second Hospital of Shandong UniversityJinan 250000Shandong ProvinceChina State Key Laboratory for Diagnosis and Treatment of Infectious Diseases Collaborative Innovation Center for Diagnosis and Treatment of Infectious DiseaseThe First Affiliated HospitalZhejiang University School of MedicineHangzhou 310000Zhejiang ProvinceChina Department of Infectious Diseases The Thir

BACKGROUND Acute-on-chronic liver disease(AoCLD)accounts for the majority of patients hospitalized in the Department of Hepatology or Infectious *** To explore the characterization of AoCLD to provide theoretical guidance for the accurate diagnosis and prognosis of *** Patients with AoCLD from the Chinese Acute-on-Chronic Liver Failure(ACLF)study cohort were included in this *** clinical characteristics and outcomes,and the 90-d survival rate associated with each clinical type of AoCLD were analyzed,using the Kaplan-Meier method and the log-rank *** A total of 3375 patients with AoCLD were enrolled,including 1679(49.7%)patients with liver cirrhosis acute decompensation(LC-AD),850(25.2%)patients with ACLF,577(17.1%)patients with chronic hepatitis acute exacer-bation(CHAE),and 269(8.0%)patients with liver cirrhosis active phase(LC-A).The most common cause of chronic liver disease(CLD)was HBV infection(71.4%).The most common precipitants of AoCLD was bacterial infection(22.8%).The 90-d mortality rates of each clinical subtype of AoCLD were 43.4%(232/535)for type-C ACLF,36.0%(36/100)for type-B ACLF,27.0%(58/215)for type-A ACLF,9.0%(151/1679)for LC-AD,3.0%(8/269)for LC-A,and 1.2%(7/577)for *** HBV infection is the main cause of CLD,and bacterial infection is the main precipitant of *** most common clinical type of AoCLD is *** diagnosis and timely intervention are needed to reduce the mortality of patients with LC-AD or ACLF.

关键词： Acute-on-chronic liver disease Acute-on-chronic liver failure Liver cirrhosis Clinical features Prognosis

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Deceased donor liver transplantation for Budd–Chiari syndrome:Long-segmental thrombosis of the inferior vena cava with extensive collateral circulation

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Hepatobiliary & Pancreatic Diseases International 2020年第3期19卷 288-290页

作者： Zi-Xi Liu Ji-Qiao Zhu Jun Ma Jian-tao Kou Xian-liang Li Qiang He Department of Hepatobiliary and Pancreaticosplenic Surgery Beijing Chaoyang HospitalCapital Medical UniversityNo.8 Gongtinan RoadChaoyang DistrictBeijing 100020China

Budd–Chiari syndrome(BCS)is an infrequent clinical disease resulting from obstruction of the hepatic venous outflow tract anywhere from small hepatic veins to the inferior vena cava(IVC)[1].Currently,step-wise treatment strategy has been proposed and widely adopted based on the patient's status,which contains anticoagulation,thrombolysis,percutaneous recanalization,transjugular intrahepatic portosystemic shunt(TIPS)and surgical shunt[2].

关键词： Chiari hepatic vena

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神经内分泌癌继发的膜性肾病

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肾脏病与透析肾移植杂志 2024年第4期33卷 393-399页

作者：蒋知凡邱丹丹梁丹丹刘正钊张涛江苏省人民医院(南京医科大学第一附属医院)老年肾科南京210029 东部战区总医院国家肾脏疾病临床医学研究中心

老年男性患者,因“反复双下肢水肿13年,再发半月”入院,伴随原发灶未明的低分化神经内分泌癌病史2年,两次肾活检皆提示膜性肾病,13年前肾活检组织抗磷脂酶A2受体(PLA2R)荧光染色阳性,Ⅰ型血小板域蛋白7A(THSD7A)染色阴性,此次肾活检组织... 详细信息

老年男性患者,因“反复双下肢水肿13年,再发半月”入院,伴随原发灶未明的低分化神经内分泌癌病史2年,两次肾活检皆提示膜性肾病,13年前肾活检组织抗磷脂酶A2受体(PLA2R)荧光染色阳性,Ⅰ型血小板域蛋白7A(THSD7A)染色阴性,此次肾活检组织PLA2R染色阴性,THSD7A染色阳性,转移性腹股沟淋巴结活检组织THSD7A染色阳性,结合临床、实验室检查及病理最终诊断为神经内分泌癌继发膜性肾病(THSD7A相关),该疾病系国内首次报道。

关键词：神经内分泌癌继发性膜性肾病 Ⅰ型血小板域蛋白7A

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The relationship between human papillomavirus and penile cancer over the past decade:a systematic review and meta-analysis

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Asian Journal of Andrology 2019年第4期21卷 375-380页

作者： Yong-Bo Yu Yong-Hua Wang Xue-Cheng Yang Yang Zhao Mei-Lan Wang Ye liang Hai-tao Niu Department of Urology Affiliated Hospital of Qingdao University Qingdao 266003 China The Nursing Department Qingdao University Qingdao 266021 China

Human papillomavirus (HPV) infection appears to play an important role in the development of penile cancer (PeCa), but their relationship remains unclear. Therefore, we performed a systematic review and meta-analysis to elucidate their relationship. We systematically searched Embase, PubMed, Cochrane Library, and Web of Science for case-control studies and cross-sectional studies using polymerase chain reaction (PCR) tech no logy on formali n?fixed paraffi n-embedded (FFPE) or pa raff i n-embedded (PE) PeCa tissues to detect HPV (published between January 1, 2007, and December 29, 2017;no language restrictions). Twenty-two studies were identified, and 1664 cases were available for analysis. The combined HPV infectious risk of PeCa is 51.0%(95% confidence interval [Cl]: 43.0%-60.0%). The three most common subtypes of HPV were HPV16 (28.5%), HPV18 (2.3%), and HPV6 (2.3%). The virus was relevantly associated with basaloid (85.5%, 95% Cl: 77.2%-93.8%) and warty (50.0%, 95% Cl: 35.2%-64.8%) carcinomas. The invasiveness of PeCa was not associated with HPV (X^2 = 0.181, df = 1, P< 0.671). HPV infection in PeCa tended to be moderately differentiated (54.4%, 95% Cl: 47.7%-61.1%). This study found that almost half of PeCa patients are associated with HPV. The most comm only associated geno type is HPV16, but several other geno types were also detected. In addition to types 6 and 11, other single low-risk HPV infections have been found to contribute to PeCa to a lesser degree. HPVpositive tumors tend to exhibit warty and/or basaloid features, corresponding to a moderate histological grade. The role of HPV in PeCa should be revisited to provide evidenee for the development of PeCa in the presenee of HPV infection.

关键词： human papillomavirus penile cancer systematic review and meta-analysis

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水下控制模块应急解锁工具设计与分析

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机电设备 2024年第1期41卷 30-35页

作者：杜振伟闫嘉钰王文聪熊涛李伟胡亮重庆前卫科技集团有限公司重庆401121

水下控制模块(SCM)是水下生产设备的核心单元,随着作业水深增加,必须采用专用工具才能实现可靠安装。对重庆前卫生产的QSCMⅡ代水下控制模块的锁紧及解锁过程进行分析,设计一个专用于其应急解锁的工具,从而实现其水下应急解锁。在此基础... 详细信息

水下控制模块(SCM)是水下生产设备的核心单元,随着作业水深增加,必须采用专用工具才能实现可靠安装。对重庆前卫生产的QSCMⅡ代水下控制模块的锁紧及解锁过程进行分析,设计一个专用于其应急解锁的工具,从而实现其水下应急解锁。在此基础上,构建应急解锁工具驱动杆的有限元分析模型,并对驱动杆和连接螺纹的强度进行分析。研究结果表明:驱动杆最大应力位于最细轴颈处,最大应力为91.736 MPa,远小于材料屈服强度517.000 MPa;螺纹连接最大接触压力位于末端螺纹处,为24.176 MPa,小于螺纹材料的许用挤压应力。SCM应急解锁工具进行调试,满足设计要求,为以后水下控制模块及相关产品的设计优化提供思路。

关键词：水下控制模块(SCM) 应急解锁工具设计有限元分析

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AMPK activation by peri-sciatic nerve administration of ozone attenuates CCl-induced neuropathic pain in rats

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Journal of Molecular Cell Biology 2017年第2期9卷 132-143页

作者： Lijuan Lu Cailong Pan Lu Chen liang Hu Chaoyu Wang Yuan Han Yanjing Yang Zhixiang Cheng Wen-tao Liu Department of Pain Affiliated Drum Tower Hospital of Medical School of Nanjing University Nanjing 210008 China Jiangsu Key Laboratory of Neurodegeneration Department of Pharmacology Nanjing Medical University Nanjing 210029 China State Key Laboratory of Natural Medicines China Pharmaceutical University Nanjing 210009 China Jiangsu Key Laboratory of Oral Diseases Nanjing Medical University Nanjing 210029 China Department of Pain Management & Cancer Biotherapy Center The Second Affiliated Hospital of Nanjing Medicat University Nanjing 210029 China

Neuropathic pain is a debilitating clinical condition with few efficacious treatments, warranting development of novel therapeu- tics. Ozone is widely used as an alternative therapy for many different pain conditions, with exact mechanisms still elusive. In this study, we found that a single peri-sciatic nerve injection of ozone decreased mechanical aUodynia and thermal hyperalgesia, and normalized the phosphorylation of protein kinase C y, N-methyI-D-aspartate receptor, and extracellular signal-regulated kinase in a chronic constriction injury （CCI） model in rat sciatic nerve. Meanwhile, ozone significantly suppressed CCI-induced activation of spinal microgUa. More importantly, the anti-nociceptive effect of ozone depended on the activation of 5＇-adenosine monophosphate （AMP）-activated protein kinase （AMPK）, which was proved by the fact that the phosphorylated AMPK level increased during the ozone therapy and AMPK antagonist abolished the effect of ozone in vivo and in vitro. In addition, direct injection of AMPK agonist could replicate the anti-nociceptive effect of ozone in CCI rats. In conclusion, our observations indicate that peri-sciatic nerve injection of ozone activates AMPK to attenuate CCI-induced neuropathic pain.

关键词： AMPK ozone therapy neuropathic pain

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Neuroprotective effects of a ketogenic diet in combination with exogenous ketone salts following acute spinal cord injury

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Neural Regeneration Research 2020年第10期15卷 1912-1919页

作者： Bo-tao Tan Hui Jiang Aaron JMoulson Xiao-liang Wu Wen-Chun Wang Jie Liu Ward TPlunet Wolfram Tetzlaff Department of Rehabilitation Medicine The Second Affiliated Hospital of Chongqing Medical UniversityChongqingChina International Collaboration on Repair Discoveries(ICORD) Blusson Spinal Cord CenterVancouverBritish ColumbiaCanada Department of Zoology University of British ColumbiaVancouverBritish ColumbiaCanada Department of Spinal Surgery Nanfang HospitalSouthern Medical UniversityGuangzhouGuangdong ProvinceChina Department of Rehabilitation Medicine The General Hospital of Western Theater Command PLAChengduSichuan ProvinceChina

We have previously shown that induction of ketosis by ketogenic diet(KD)conveyed neuroprotection following spinal cord injury in rodent models,however,clinical translation may be limited by the slow raise of ketone levels when applying KD in the acute post-injury *** we investigated the use of exogenous ketone supplementation(ketone sodium,KS)combined with ketogenic diet as a means rapidly inducing a metabolic state of ketosis following spinal cord injury in adult *** uninjured rats,ketone levels increased more rapidly than those in rats with KD alone and peaked at higher levels than we previously demonstrated for the KD in models of spinal cord ***,ketone levels in KD+KS treated rats with SCI did not exceed the previously observed levels in rats treated with KD *** still demonstrated neuroprotective effects of KD+KS treatment that extend our previous neuroprotective observations with KD *** results showed increased neuronal and axonal sparing in the dorsal corticospinal ***,better performance of forelimb motor abilities were observed on the Montoya staircase(for testing food pellets reaching)at 4 and 6 weeks post-injury and rearing in a cylinder(for testing forelimb usage)at 6 and 8 weeks *** together,the findings of this study add to the growing body of work demonstrating the potential benefits of inducing ketosis following *** salt combined with a ketogenic diet gavage in rats with acute spinal cord injury can rapidly increase ketone body levels in the blood and promote motor function *** study was approved by the Animal Care Committee of the University of British Columbia(protocol No.A14-350)on August 31,2015.

关键词： axon behavior corticospinal tract ketogenic diet ketone supplementation myelin neuroprotection spinal cord injury

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喷涂沉积纳米纤维素包装膜的研究进展

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应用化工 2024年第4期53卷 877-882页

作者：许妍妞李名洋吴训潇刘涛梁容榕曾亚萍何焕杰陆登俊广西大学轻工与食品工程学院广西南宁530004 广西大学甘蔗与制糖产业学院广西南宁530004

根据纳米纤维素包装膜常规制备方法存在的连续性差、生产时间长、膜可操控性差等问题,从机械性能、阻氧性能、防潮性能方面综述喷涂沉积法制备纳米纤维素包装膜机理及包装应用优势。喷涂沉积法制备纳米纤维素包装膜的研究为快速有效地... 详细信息

根据纳米纤维素包装膜常规制备方法存在的连续性差、生产时间长、膜可操控性差等问题,从机械性能、阻氧性能、防潮性能方面综述喷涂沉积法制备纳米纤维素包装膜机理及包装应用优势。喷涂沉积法制备纳米纤维素包装膜的研究为快速有效地制备纳米纤维素包装膜,保证生产程序的连续性,更好地实现在食品、生物医学、军事等方面的包装领域应用,为新型包装材料提供理论基础并进行展望。

关键词：纤维素喷涂沉积法纳米纤维素膜食品应用包装生物医用包装军事应用包装

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