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Prediction of cell-cell communication patierns of dorsal root ganglion cells:single-cell RNA sequencing data analysis

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Neural Regeneration Research 2024年第6期19卷 1367-1374页

作者： Yanna lian Cheng Wu li liu Xiangyao li National Health Commission and Chinese Academy of Medical Sciences Key Laboratory of Medical Neurobiology Ministry of Education Frontier Science Center for Brain Research and Brain-Machine IntegrationSchool of Brain Science and Brain MedicineZhejiang UniversityHangzhouZhejiang ProvinceChina International Institutes of Medicine the Fourth Affiliated Hospital of Zhejiang University School of MedicineYiwuZhejiang ProvinceChina Zhejiang University-University of Edinburgh Institute Zhejiang University School of MedicineZhejiang UniversityHainingZhejiang ProvinceChina Biomedical Sciences College of Medicine and Veterinary MedicineThe University of EdinburghEdinburghUK Core Facilities of the School of Medicine Zhejiang UniversityHangzhouZhejiang ProvinceChina

Dorsal root ganglion neurons transmit peripheral somatic information to the central nervous system,and dorsal root ganglion neuron excitability affects pain *** root ganglion stimulation is a new approach for managing pain *** of the cell-cell communication among dorsal root ganglion cells may help in the development of new pain and itch management ***,we used the single-cell RNA-sequencing(scRNA-seq)database to investigate intercellular communication networks among dorsal root ganglion *** collected scRNA-seq data from six samples from three studies,yielding data on a total of 17,766 *** on genetic profiles,we identified satellite glial cells,Schwann cells,neurons,vascular endothelial cells,immune cells,fibroblasts,and vascular smooth muscle *** analysis revealed that eight types of dorsal root ganglion neurons mediated proprioceptive,itch,touch,mechanical,heat,and cold ***,we predicted several distinct forms of intercellular communication among dorsal root ganglion cells,including cell-cell contact,secreted signals,extracellular matrix,and neurotransmitter-mediated *** data mining predicted that Mrgpra3-positive neurons robustly express the genes encoding the adenosine Adora2b(A2B)receptor and glial cell line-derived neurotrophic factor family receptor alpha 1(GFRα-1).Our immunohistochemistry results confirmed the coexpression of the A2B receptor and GFRα-*** injection of the A2B receptor antagonist PSB-603 effectively prevented histamine-induced scratching behaviour in a dose-dependent *** results demonstrate the involvement of the A2B receptor in the modulation of itch ***,our findings provide insight into dorsal root ganglion cell-cell communication patterns and *** results should contribute to the development of new strategies for the regulation of dorsal root ganglion excitability.

关键词： cell-cell communications CellChat dorsal root ganglion scRNA-Seq database

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Morphogenesis and Molecular Phylogeny of a Marine Urostylid Ciliate Apokeronopsis wrighti(Protista, Ciliophora, Hypotrichia)

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Journal of Ocean University of China 2024年第4期23卷 1067-1075页

作者： lian Chunyu li Ping ZHANG Tengteng SHAO Chen Department of Zoology College of Life SciencesShaanxi Normal UniversityXi’an 710119China Powerchina Northwest Engineering Corporation limited Xi’an 710065China

Hypotrichs are one of the highly differentiated ciliated lineages which play important roles in ecological, environmental,evolutionary and basic biological studies. In the present study, we investigated the living characteristics, infraciliature, nuclear apparatus, ontogenesis and phylogenetic position of a marine hypotrichous ciliate, Apokeronopsis wrighti Long et al., 2008, which was isolated from coastal waters in Shenzhen, China. The new isolate resembles the type population in terms of morphological characteristics, morphometrics, and SSU rRNA gene sequence that is with a 99.7% similarity. Ontogenesis of A. wrighti is characterized by oral primordium for the proter as well as marginal and dorsal kineties anlagen in both filial products formed de novo, and the cirral row arranged along the paroral and endoral arises from several anterior frontoventral-transverse cirral streaks. Phylogenetic analyses based on SSU and concatenated gene data suggest that five species of Apokeronopsis form a monophyletic clade, and the genus Apokeronopsis is closely related to Thigmokeronopsis and Metaurostylopsis.

关键词： 18S rRNA gene Apokeronopsis ciliated protists multi-gene ontogenesis systematics Urostylida

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Influence of Incomplete Soil Plugs on Bearing Capacities of Bucket Foundations in Clay

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China Ocean Engineering 2024年第1期38卷 144-155页

作者： li Hui-shan liU Run YANG Xu lian Ji-jian State Key Laboratory of Hydraulic Engineering Intelligent Construction and Operation Tianjin UniversityTianjin 300072China School of Civil Engineering Tianjin UniversityTianjin 300072China Institute of Marine Energy and Intelligent Construction Tianjin University of TechnologyTianjin 300384China

Due to the uneven seabed and heaving of soil during pumping,incomplete soil plugs may occur during the installation of bucket foundations,and the impacts on the bearing capacities of bucket foundations need to be *** this paper,the contact ratio(the ratio of the top diameter of the soil plug to the diameter of the bucket)and the soil plug ratio(the ratio of the soil heave height to the skirt height)are defined to describe the shape and size of the incomplete soil ***,finite element models are established to investigate the bearing capacities of bucket foundations with incomplete soil plugs and the influences of the contact ratios,and the soil plug ratios on the bearing capacities are *** results show that the vertical bearing capacity of bucket foundations in homogeneous soil continuously improves with the increase of the contact ***,in normally consolidated soil,the vertical bearing capacity barely changes when the contact ratio is smaller than 0.75,while the bearing capacity suddenly increases when the contact ratio increases to 1 due to the change of failure *** contact ratio hardly affects the horizontal bearing capacity of bucket ***,the moment bearing capacity improves with the increase of the contact ratio for small aspect ratios,but hardly varies with increasing contact ratio for aspect ratios larger than ***,the reduction coefficient method is proposed based on this analysis to calculate the bearing capacities of bucket foundations considering the influence of incomplete soil *** comparison results show that the proposed reduction coefficient method can be used to evaluate the influences of incomplete soil plug on the bearing capacities of bucket foundations.

关键词： bucket foundation incomplete soil plug uniaxial bearing capacity contact ratio soil plug ratio

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Parity-dependent skin effects and topological properties in the multilayer nonreciprocal Su−Schrieffer−Heeger structures

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Frontiers of physics 2024年第3期19卷 115-133页

作者： Jia-Rui li Cui Jiang Han Su Di Qi lian-lian Zhang Wei-Jiang Gong College of Sciences Northeastern UniversityShenyang 110819China Basic Department Shenyang Institute of EngineeringShenyang 110136China

We concentrate on the skin effects and topological properties in the multilayer non-Hermitian Su−Schrieffer−Heeger (SSH) structure, by taking into account the nonreciprocal couplings between the different sublattices in the unit cells. Following the detailed demonstration of the theoretical method, we find that in this system, the skin effects and topological phase transitions induced by nonreciprocal couplings display the apparent parity effect, following the increase of the layer number of this SSH structure. On the one hand, the skin effect is determined by the parity of the layer number of this SSH system, as well as the parity of the band index of the bulk states. On the other hand, for the topological edge modes, such an interesting parity effect can also be observed clearly. Next, when the parameter disorders are taken into account, the zero-energy edge modes in the odd-layer structures tend to be more robust, whereas the other edge modes are easy to be destroyed. In view of these results, it can be ascertained that the findings in this work promote to understand the influences of nonreciprocal couplings on the skin effects and topological properties in the multilayer SSH lattices.

关键词： multilayer SSH lattice nonreciprocal couplings band structure skin effect

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A new 4-Ti-added polyoxometalate

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Tungsten 2024年第2期6卷 465-471页

作者： Hai-Lou li Chen lian Guo-Yu Yang MOE Key Laboratory of Cluster Science School of Chemistry and Chemical EngineeringBeijing Institute of TechnologyBeijing 102488China

A new inorganic–organic hybrid 4-Ti-added polyoxometalate:[H_(2)N(CH_(3))_(2)]_(2)Na_(3)H_(5)[{Ti(C_(2)O_(4))(H_(2)O)}_(2){Ti(C_(2)O_(4))}_(2)(B-β-SbW_(9)O_(33))_(2)]·22H_(2)O(1)has been successfully made and further characterized by infrared spectroscopy,thermogravimetric analysis,powder X-ray diff raction and single-crystal X-ray diff *** polyoxoanion of 1 exhibits a Krebs-type structure that is constructed from two[B-β-SbW_(9)O_(33)]^(9)−fragments fused together by a belt of four additional Ti atoms decorated by oxalate ***,1 as a heterogeneous catalyst exhibits high activity for Knoevenagel condensation under mild reaction conditions.

关键词： Polyoxometalate Ti-added POM Krebs-type structure Knoevenagel condensation

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Suppression of current-induced membrane discharge of bipolar membranes by regulating ion crossover transport

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Journal of Energy Chemistry 2024年第9期96卷 387-395页

作者： Tingting Yu Haolan Tao Jingkun li Cheng lian Honglai liu State Key Laboratory of Chemical Engineering Shanghai Engineering Research Center of Hierarchical NanomaterialsSchool of Chemical EngineeringEast China University of Science and TechnologyShanghai 200237China School of Chemistry and Molecular Engineering East China University of Science and TechnologyShanghai 200237China

Bipolar membranes(BPMs)exhibit the unique capability to regulate the operating environment of electrochemical system through the water dissociation-combination ***,the industrial utilization of BPMs is limited by instability and serious energy *** current-induced membrane discharge(CIMD)at high-current conditions has a negative influence on the performance of anion-exchange membranes,but the underlying ion transport mechanisms in the BPMs remain ***,the CIMD-coupled Poisson-Nernst-Planck(PNP)equations are used to explore the ion transport mechanisms in the BPMs for both reverse bias and forward bias at neutral and acid-base *** is demonstrated that the CIMD effect in the reverse-bias mode can be suppressed by enhancing the diffusive transport of salt counter-ions(Na^(+)and Cl^(−))into the BPMs,and that in the forward-bias mode with acid-base electrolytes can be suppressed by matching the transport rate of water counter-ions(H_(3)O^(+)and OH^(−)).Suppressing the CIMD can promote the water dissociation in the reverse-bias mode,as well as overcome the plateau of limiting current density and reduce the interfacial blockage of salt co-ions(Cl^(−))in the anion-exchange layer in the forward-bias mode with acid-base *** work highlights the importance of regulating ion crossover transport on improving the performance of BPMs.

关键词： Bipolar membranes Current-induced membrane discharge Salt ion crossover Diffusion-migration-reaction process

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Molecularly imprinted sensor based on carboxylated carbon nanotubes/Keggin-type polyoxometalates nanocomposite for the detection of furazolidone

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Tungsten 2024年第2期6卷 394-409页

作者： Yu-Lan Huang Bing Zhang Yu-lian li li Wang Le Dong Jian li College of Ocean Food and Biological Engineering Jimei UniversityXiamen 361021China College of Oceanology and Food Science Quanzhou Normal UniversityQuanzhou 362000China

Due to its properties of mutagenic,teratogenic,and carcinogenic,the detection of furazolidone(FZD)in aquaculture is of great importance for food safety and human *** this study,molecularly imprinted fi lms modifi ed with carboxylated multi-walled carbon nanotube-phosphomolybdic acid composite were used to fabricate an electrochemical sensor for the determination of *** nanocomposites were characterized using infrared spectroscopy,scanning electron microscopy,energy-dispersive X-ray spectroscopy,and X-ray diff *** electrochemical characteristics of the modifi ed electrodes were examined using electrochemical impedance spectroscopy,cyclic voltammetry,and diff erential pulse *** sensor exhibited exceptional catalytic *** calibration curves were acquired in the concentration range of 6 nmol·L^(−1)to 0.6μmol·L^(−1),with a limit of detection of 3.38 nmol·L^(−1).Additionally,the sensor proved successful in recognizing FZD in shrimp samples with satisfactory recoveries and *** method provides a strategy to construct a molecularly imprinted electrochemical sensing platform using nanomaterials,which has great promise in the field of food safety.

关键词： Phosphomolybdate Furazolidone Polyoxometalates Carboxylated multi-walled carbon nanotube Molecularly imprinted polymers Electrochemical sensor

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Acid-rock reaction kinetics in a two-scale model based on reaction order correction

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Petroleum Science 2024年第3期21卷 2089-2101页

作者： Xue-Song li Ning Qi Ze-Hui Zhang lian liu Xia-Qing li Xu-Hang Su School of Petroleum Engineering China University of Petroleum(East China)Qingdao266580ShandongChina Key Lab of Unconventional Oil&Gas Development Ministry of EducationChina University of Petroleum(East China)Qingdao26650ShandongChina SINOPEC Northwest Oilfield Branch Company Urumqi830011XinjiangChina Petroleum Engineering Technology Research Institute SINOPEC Shengli Oilfield CompanyDongying257000ShandongChina

The reaction order plays a crucial role in evaluating the response rate of ***,the conventional two-scale model typically assumes that the reaction order is constant as one,which can lead to significant deviations from *** address this issue,this study proposes a novel multi-order dynamic model for acid-rock reaction by combining rotating disk experimental data with theoretical *** numerical simulations,this model allows for the investigation of the impact of acidification conditions on different orders of reaction,thereby providing valuable insights for on-site *** analysis reveals that higher response orders require higher optimal acid liquid flow rates,and lower optimal H+diffusion coefficients,and demonstrate no significant correlation with acid ***,it is recommended to increase the displacement and use high-viscosity acid for reservoirs with high calcite content,while reducing the displacement and using low-viscosity acid for reservoirs with high dolomite content.

关键词： Reaction order Two-scale model Wormhole propagation Carbonate rocks Numerical simulation

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Dissolvable temporary barrier:a novel paradigm for flexible hydrogel patterning in organ-on-a-chip models

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Bio-Design and Manufacturing 2024年第2期7卷 153-166页

作者： Ding Wang Qinyu li Chenyang Zhou Zhangjie li Kangyi Lu Yijun liu lian Xuan Xiaolin Wang Department of Micro/Nano Electronics School of Electronic Information and Electrical EngineeringShanghai Jiao Tong UniversityShanghai 200240China Department of Ophthalmology LKS Faculty of MedicineThe University of Hong KongHong Kong 999077China Institute of Medical Robotics Shanghai Jiao Tong UniversityShanghai 200240China National Key Laboratory of Advanced Micro and Nano Manufacture Technology Shanghai Jiao Tong UniversityShanghai 200240China National Center for Translational Medicine(Shanghai)SHU Branch Shanghai UniversityShanghai 200444China

A combination of hydrogels and microfluidics allows the construction of biomimetic three-dimensional(3D)tissue models in vitro,which are also known as *** hydrogel patterningwith awell-controlled spatial distribution is typically achieved by embedding sophisticated microstructures to act as a ***,these physical barriers inevitably expose cells/tissues to a less physiologically relevant microenvironment than in vivo ***,we present a novel dissolvable temporary barrier(DTB)strategy that allows robust and flexible hydrogel patterning with great freedom of design and desirable flow stimuli for cellular *** key aspect of this approach is the patterning of a water-soluble rigid barrier as a guiding path for the hydrogel using stencil printing technology,followed by a barrier-free medium perfusion after the dissolution of the *** and multiple tissue compartments with different geometries can be established using either straight or curved DTB *** effectiveness of this strategy is further validated by generating a 3D vascular network through vasculogenesis and angiogenesis using a vascularized microtumor *** a new proof-of-concept in vasculature-on-a-chip,DTB enables seamless contact between the hydrogel and the culture medium in closed microdevices,which is an improved protocol for the fabrication ofmultiorgan ***,we expect it to serve as a promising paradigm for organ-on-a-chip devices for the development of tumor vascularization and drug evaluation in the future preclinical studies.

关键词： Dissolvable temporary barrier Hydrogel patterning Microfluidics Organ-on-a-chip Vascularization

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Cycloastragenol induces apoptosis and protective autophagy through AMPK/ULK1/mTOR axis in human non-small cell lung cancer cell lines

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Journal of Integrative Medicine 2024年第4期22卷 503-514页

作者： li-hua Zhu Yu-pei liang lian Yang Feng Zhu li-jun Jia He-gen li Department of Oncology Longhua HospitalShanghai University of Traditional Chinese MedicineShanghai 200032China Cancer Institute of Traditional Chinese Medicine Longhua HospitalShanghai University of Traditional Chinese MedicineShanghai 200032China Department of Laboratory Medicine Huadong HospitalFudan UniversityShanghai 200237China

Objective:Studies have demonstrated that cycloastragenol induces antitumor effects in prostate,colorectal and gastric cancers;however,its efficacy for inhibiting the proliferation of lung cancer cells is largely *** study explores the efficacy of cycloastragenol for inhibiting non-small cell lung cancer(NSCLC)and elucidates the underlying molecular ***:The effects of cycloastragenol on lung cancer cell proliferation were assessed using an adenosine triphosphate monitoring system based on firefly luciferase and clonogenic formation ***-induced apoptosis in lung cancer cells was evaluated using dual staining flow cytometry with an annexin V-fluorescein isothiocyanate/propidium iodide *** elucidate the role of cycloastragenol in the induction of apoptosis,apoptosis-related proteins were examined using Western *** and Western blotting were used to determine whether cycloastragenol could induce autophagy in lung cancer *** techniques,including small interfering RNA technology,were used to investigate the underlying *** effects against lung cancer and biosafety of cycloastragenol were evaluated using a mouse subcutaneous tumor ***:Cycloastragenol triggered both autophagy and ***,cycloastragenol promoted apoptosis by facilitating the accumulation of phorbol-12-myristate-13-acetate-induced protein 1(NOXA),a critical apoptosis-related ***,cycloastragenol induced a protective autophagy response through modulation of the adenosine 5'-monophosphate-activated protein kinase(AMPK)/unc-51-like autophagy-activating kinase(ULK1)/mammalian target of rapamycin(mTOR)***:Our study sheds new light on the antitumor efficacy and mechanism of action of cycloastragenol in *** insight provides a scientific basis for exploring combination therapies that use cycloastragenol and inhibiting the AMPK/ULK1/mTOR pathway as a promising approach to

关键词： Non-small cell lung cancer Cycloastragenol Autophagy Apoptosis

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