Dissolvable temporary barrier:a novel paradigm for flexible hydrogel patterning in organ-on-a-chip models

作     者：Ding Wang Qinyu Li Chenyang Zhou Zhangjie Li Kangyi Lu Yijun Liu Lian Xuan Xiaolin Wang Ding Wang;Qinyu Li;Chenyang Zhou;Zhangjie Li;Kangyi Lu;Yijun Liu;Lian Xuan;Xiaolin Wang

作者机构：Department of Micro/Nano ElectronicsSchool of Electronic Information and Electrical EngineeringShanghai Jiao Tong UniversityShanghai 200240China Department of OphthalmologyLKS Faculty of MedicineThe University of Hong KongHong Kong 999077China Institute of Medical RoboticsShanghai Jiao Tong UniversityShanghai 200240China National Key Laboratory of Advanced Micro and Nano Manufacture TechnologyShanghai Jiao Tong UniversityShanghai 200240China National Center for Translational Medicine(Shanghai)SHU BranchShanghai UniversityShanghai 200444China 

出 版 物：《Bio-Design and Manufacturing》 (生物设计与制造（英文）)

年 卷 期：2024年第7卷第2期

页      面：153-166页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 08[工学] 0836[工学-生物工程] 

基　　金：supported by the National Natural Science Foundation of China(Nos.31972929 and 62231025) the Research Program of Shanghai Science and Technology Committee(Nos.21140901300 and 20DZ2220400) the Natural Science Foundation of Chongqing,China(No.CSTB2022NSCQ-MSX0767) the Interdisciplinary Program of Shanghai Jiao Tong University(Nos.YG2021ZD22 and YG2023LC04) the Foundation of National Center for Translational Medicine(Shanghai)SHU Branch(No.SUITM-2023008) the Cross-disciplinary Research Fund of Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine(No.JYJC202108) 

主　　题：Dissolvable temporary barrier Hydrogel patterning Microfluidics Organ-on-a-chip Vascularization 

摘      要：A combination of hydrogels and microfluidics allows the construction of biomimetic three-dimensional(3D)tissue models in vitro,which are also known as *** hydrogel patterningwith awell-controlled spatial distribution is typically achieved by embedding sophisticated microstructures to act as a ***,these physical barriers inevitably expose cells/tissues to a less physiologically relevant microenvironment than in vivo ***,we present a novel dissolvable temporary barrier(DTB)strategy that allows robust and flexible hydrogel patterning with great freedom of design and desirable flow stimuli for cellular *** key aspect of this approach is the patterning of a water-soluble rigid barrier as a guiding path for the hydrogel using stencil printing technology,followed by a barrier-free medium perfusion after the dissolution of the *** and multiple tissue compartments with different geometries can be established using either straight or curved DTB *** effectiveness of this strategy is further validated by generating a 3D vascular network through vasculogenesis and angiogenesis using a vascularized microtumor *** a new proof-of-concept in vasculature-on-a-chip,DTB enables seamless contact between the hydrogel and the culture medium in closed microdevices,which is an improved protocol for the fabrication ofmultiorgan ***,we expect it to serve as a promising paradigm for organ-on-a-chip devices for the development of tumor vascularization and drug evaluation in the future preclinical studies.