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Cryopreservation of Queen Honeybee (Apis mellifera carnica) Born Worker Eggs by Vitrification

Agricultural Sciences in China 2010年第9期9卷 1353-1361页

作者： li zhi-yong XUE Yun-bo WANG zhi li xing-an Key Laboratory for Honeybee Genetics and Breeding Jilin Provincial Apiculture Institute Jilin 132108 P.R. China

Many species of insect egg can be targeted individually or （and） collectively for cryopreservation by vitrification. However, there has been no report on cryopreservation of honeybee eggs by vitrification. In an attempt to define a preliminary procedure of cryopreservation of honeybee eggs by vitrification, queen honeybee born worker eggs （worker eggs） were stored through vitrification in liquid nitrogen up to 1 h, and then post-vitrification survival of the vitrified worker eggs in vitro and their hatching rates during maturation in vivo were observed using microscopic and close visual inspections. The procedure of cryopreservation by vitrification included dechorionation with sodium hypochlorite and permeabilization with isopropyl alcohol; equilibration by addition of loading solution （i.e., 25% vitrification storage solution） and dehydration by gradual replacement of loading solution with vitrification storage solution; cooling in liquid nitrogen vapor right before droplet vitrification in liquid nitrogen; and recovery in liquid nitrogen vapor right after storage in liquid nitrogen, thawing at temperature of thawing medium （5% sucrose in TC 100-insect medium） and rehydration by gradual replacement of vitrification storage solution with rehydration solution （5% fetal bovine serum in TC 100-insect medium）. It was found that among the worker eggs experiencing cyropreservation by vitrification, 1.25% of them were successfully passed through the four life stages, viz., egg, larva, pupa, and adult. In summary, it can be inferred that although a majority of worker eggs were dead after cyropreservation by vitrification, a few of them were developed into larvae, pupae, and finally emerged as adults.

关键词： honeybee worker egg cryopreservation vitrification

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Late Pleistocene aeolian activity in Haitan Island, Southeast China: Insights from optically stimulated luminescence dating of coastal dunes on marine terraces

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Journal of Mountain Science 2018年第8期15卷 1777-1788页

作者： JIN Jian-hui li zhi-zhong CHENG Yan XU Xiao-lin li zhi-xing liU Xiao-ju Institute of Geography Fujian Normal University Key Laboratory for Humid Subtropical Eco-geographical Processes of the Ministry of Education Fujian Normal University College of Geographical Sciences Fujian Normal University

The 'Old Red Sand' is a type of semicemented medium-fine sandy sediment that is red(10R_4/8) or brown red(2.5YR_4/8) in colour and is found in late Quaternary deposits. The sediments have distinctive characteristics and are a critical archive for understanding climatic changes in the coastal areas of East Asia. The ages of the late Quaternary aeolian sand dunes from Haitan Island in the coastal area of South China are still in debate. In this study, three sets of marine terraces were identified in the northern region of Haitan Island. Aeolian dune sands are well preserved on the top of these terraces. Quartz Optically Stimulated Luminescence dating and the distribution of the formation ages demonstrated that the palaeo-dunes are deposits from the middle-late period of the Late Pleistocene(Q_3^(2-3)). The period may be divided into three stages, 100-90 ka, 70-60 ka, and 40-20 ka, in which the palaeo-dunes of the first two stages are more widespread and were formed separately during a low-sea level period of the Marine Isotope Stages 5 b and 4. Several depositional palaeo-flood event records were preserved during the last stage due to the increasing gradient of mountain gullies formed during the Last Glacial Maximum.

关键词： Aeolian activity Sediment dating Optically Stimulated Luminescence (OSL) Late Pleistocene Environmental evolution Haitan Island

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Chronology of coastal aeolian deposition and its paleo-environmental implications on the liuao Peninsula of South China

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Journal of Mountain Science 2019年第12期16卷 2754-2769页

作者： JIN Jian-hui li zhi-zhong liNG zhi-yong ZHENG Fei XU Xiao-lin CHENG Yan CAO Xiang-dong li zhi-xing ZHANG Wen-jing REN Yong-qing Key Laboratory for Humid Subtropical Eco-geographical Processes of the Ministry of Education Fujian Normal UniversityFuzhou 350007China Institute of Geography Fujian Normal UniversityFuzhou 350007China School of Geographical Sciences Fujian Normal UniversityFuzhou 350007China Qinghai Institute of Salt Lakes Chinese Academy of SciencesXi'ning 810008China Xinjiang Teacher's college(Xinjiang education Institute) Urumqi 830043China

Coastal dunes are a common geomorphic type in sandy coastal *** are a record of the coupled evolutionary processes of the wind and ocean *** coastal dune fields have developed on the east coast of Fujian China and now occur as widespread typical coastal aeolian sand landforms on the liuao Peninsula,Gulei Peninsula and Dongshan Island,but it is difficult to evaluate the dynamic geomorphologic process of sandy coast due to the lack of systematic and accurate chronological *** this study,we selected the Hutoushan(HTS)aeolian dune on the liuao Peninsula as the research *** dating and grain-size analysis were applied to sand samples from the aeolian sequence of a profile of the HTS *** results show that the ages of seven samples of this profile were in the range of 37.8–0.19 ka from 4.0 to 0.2 m *** correspond to the Marine Isotope Stage 3(MIS3),abrupt climatic change events of 4.2 and 1.1 ka and the little Ice Age(liA),*** samples displayed evidence of a longer-term climate trend in this *** period of formation of this coastal aeolian dune corresponds to a cold and arid climate associated with the East Asia Winter Monsoon(EAWM).Periods of dune fixation and rubification are evidence of a hot and humid *** and stabilization of the aeolian dune is an important characteristic of the coastal evolution in South China since the late Pleistocene.

关键词： Aeolian sand Optically Stimulated Luminescence Grain size Coastal dune Environmental evolution

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Fatty acid-binding protein gene is indispensable for molting process in Heortia vitessoides(Lepidoptera:Crambidae)

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Journal of Integrative Agriculture 2023年第2期22卷 495-504页

作者： YE Qing-ya li zhi-xing CHEN Qing-ling SUN Ming-xu YIN Ming-liang liN Tong College of Forestry and Landscape Architecture South China Agricultural UniversityGuangzhou 510642P.R.China

As intracellular fatty acid(FA) carriers,FA-binding proteins(FABPs) widely participate in the absorption,transport,and metabolism of *** is a key protein in insect lipid metabolism and plays an important role in various physiological activities of *** FABP gene(HvFABP) was cloned from the transcriptional library of Heortia vitessoides Moore(Lepidoptera:Crambidae),and its expression patterns were determined using reverse transcription quantitative PCR(RTqPCR).Stage-and tissue-specific expression profiles indicated that HvFABP highly expressed from prepupal to adult stages and in larval midgut and adult *** expression may be induced through starvation,mRNA expression was downregulated at 24 and 48 h and upregulated at 72 h after ***,20-hydroxyecdysone can induce the upregulation of its *** interference-mediated silencing of Hv FABP significantly inhibited HvFABP expression,resulting in delayed development,abnormal molting or lethal phenotypes,and a significantly reduced survival *** results indicate that HvFABP plays a key role in the molting of ***.

关键词： fatty acid-binding protein starvation 20-hydroxyecdysone RNA interference Heortia vitessoides Moore

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Effects of estrogen on collagen gel contraction by human retinal glial cells

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Chinese Medical Journal 2012年第22期125卷 4098-4103页

作者： QIU Qing-hua CHEN zhi-Yi YIN li-li ZHENG zhi WU xing-wei Shanghai Jiao Tong Univ Dept Ophthalmol Peoples Hosp 1 Shanghai 200080 Peoples R China Shanghai Heping Eye Hosp Shanghai 200437 Peoples R China

Background There are definite gender differences in patients with macular holes. Menopausal women over 50 years are most affected. We aimed to observe the effect of estrogen on collagen gel contraction by cultured human retinal glial cells. It is speculated that estrogen could strengthen the tensile stress of the macula by maintaining the correct morphology and contraction. Methods Estrogen was used to determine its effects on collagen gel contraction, and its function was measured using morphological changes in cells. Human retinal glial cells were cultured in collagen solution. The cells were then exposed to collagen gels and the degree of contraction of the gel was determined. Results Estrogen at differing concentrations had no effect on the growth of human retinal glial cells. However, after exposed to collagen gel block, less contraction was noted in the estrogen-treated group than in the control group. Conclusions Estrogen can inhibit collagen gel contraction by glial cells. These results suggest a mechanism for macular hole formation, which is observed in menopausal females.

关键词： estrogen human retinal glial cells three-dimensional culture

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军事潜力增长的无限期界模型和代际交叠模型

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系统工程理论与实践 2008年第11期28卷 171-176页

作者：李兴治张学杰炮兵学院南京分院南京211132

根据军事变量的特点和相互联系,建立军事潜力增长无限期界模型和代际交叠模型,推演最优解和平衡增长路径,线性近似解.提出和进行军事运筹学新的一类构模问题研究.尽管其中存在着一些不足,但对于传统的军事运筹学模型和国防经济学课题是... 详细信息

根据军事变量的特点和相互联系,建立军事潜力增长无限期界模型和代际交叠模型,推演最优解和平衡增长路径,线性近似解.提出和进行军事运筹学新的一类构模问题研究.尽管其中存在着一些不足,但对于传统的军事运筹学模型和国防经济学课题是有益的补充和拓展.

关键词：军事潜力增长无限期界模型代际交叠模型平衡增长路径

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A low redox potential and long life organic anode material for sodium-ion batteries

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Journal of Energy Chemistry 2025年第1期100卷 557-564页

作者： zhi li Yang Wei Kang Zhou Xin Huang xing Zhou Jie Xu Taoyi Kong Junwei Lucas Bao Xiaoli Dong Yonggang Wang Department of Chemistry and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials Institute of New Energy Collaborative Innovation Center of Chemistry for Energy Materials (iChEM) Fudan University Department of Chemistry Boston College School of Materials Science and Engineering Anhui University of Technology

Sodium-ion batteries （SIBs） with organic electrodes are an emerging research direction due to the sustainability of organic materials based on elements like C,H,O,and sodium ***,organic electrode materials for SIBs are mainly used as cathodes because of their relatively high redox potentials（>1 V）.Organic electrodes with low redox potential that can be used as anode are ***,a novel organic anode material （tetrasodium 1,4,5,8-naphthalenetetracarboxylate,Na4TDC） has been developed with low redox potential （lic *** three-sodium storage mechanism was demonstrated with various in-situ/ex-situ spectroscopy and theoretical calculations,showing a high capacity of 208 mAh/g and an average decay rate of merely 0.022%per ***,the Na4TDC-hard carbon composite can further acquire improved capacity and cycling stability for 1200 cycles even with a high mass loading of up to 20 mg *** pairing with a thick Na3V2（PO4）3cathode (20.6 mg cm-2),the as-fabricated full cell exhibited high operating voltage （2.8 V）,excellent rate performance and cycling stability with a high capacity retention of 88.7% after 200 cycles,well highlighting the Na4TDC anode material for SIBs.

关键词： Organic anode material Low redox potential Composite anode Sodium-ion batteries

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lipid-associated membrane proteins of Mycoplasma penetrans induce production of proinflammatory cytokines in human monocytic cells

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Journal of Microbiology and Immunology 2006年第4期4卷 294-299页

作者： YI MOU WU MIN JUN YU li zhi TAN XIAO xing YOU Institute of Pathobiology Medical College Nanhua University Hen6~yang P. R. China

The aim of this study is to explore potential pathogenicity of Mycoplasma penetrans, and to investigate whether M. penetrans lipid-associated membrane proteins （LAMPs） could induce human monocytic cell line （THP- 1 ） to produce some proinflammatory cytokines in vitro, including interleukin- 1β （ IL- 1β）, tumor necrosis factor alpha （TNF-α）, and IL-8. THP-1 was stimulated with different concentrations of *** LAMPs and at different time to analyze the production of human IL-1β, TNF-α and IL-8. The protein levels of human IL-1β, TNF-α and IL-8 were measured by enzyme-linked immunoadsorbent assay （EliSA） and the mRNA levels of these proinflammatory cytokines were detected by reverse transcriptase-PCR （RT-PCR）. It was demonstrated in the present study that the production of IL-1β, TNF-α and IL-8 increased in dose- and time-dependent manner after stimulation with M. penetrans LAMPs in THP-1 cells. M. penetrans LAMPs also induced the expression of IL-1β, TNF-α and IL-8 mRNA. The production of IL-1β, TNF-α and IL-8 and the expression of mRNA were down-regulated by pyrrolidine dithiocarbamate （PDTC）. This study demonstrated that M. penetrans LAMPs can induce the production of proinflammatory cytokines in human monocytic cells in vitro, thus suggesting that it may be an important etiological factor.

关键词： Mycoplasma penetrans Membrane proteins Proinflammatory cytokines

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高效耐海水型厌氧氨氧化污泥的驯化

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中国环境科学 2015年第3期35卷 748-756页

作者：李智行张蕾陈晓波李航李旦大连海洋大学海洋科技与环境学院辽宁大连116023 大连市市政设计研究院有限责任公司辽宁大连116021

针对部分含海水废水生物脱氮效能较低的问题,研究了梯度盐度海水对淡水厌氧氨氧化污泥的驯化过程.考察了不同海水盐度对厌氧氨氧化反应动力学、厌氧氨氧化菌细胞形态和反应器中菌群变化的影响.结果表明,梯度盐度废水可以成功驯化淡水厌... 详细信息

针对部分含海水废水生物脱氮效能较低的问题,研究了梯度盐度海水对淡水厌氧氨氧化污泥的驯化过程.考察了不同海水盐度对厌氧氨氧化反应动力学、厌氧氨氧化菌细胞形态和反应器中菌群变化的影响.结果表明,梯度盐度废水可以成功驯化淡水厌氧氨氧化污泥,通过145d的驯化,其总氮去除速率为2.80kg N/(m3·d).在海水盐度由0提高至10‰、20‰和30‰的过程中厌氧氨氧化反应速率经历了升高、降低、再升高的过程,其中,海水盐度20‰在淡水厌氧氨氧化污泥的驯化过程中是一个临界点.驯化后,厌氧氨氧化菌细胞结构更加不规则,并在细胞壁上出现了类菌毛状结构,经16S r DNA PCR扩增测序鉴定该优势厌氧氨氧化菌为"Candidatus Kuenenia Stuttgartiensis".驯化前后反应器中细菌菌群也发生改变.

关键词：厌氧氨氧化海水驯化脱氮

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努力培育股份制商业银行的核心竞争力

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金融理论与实践 2005年第7期 27-29页

作者：李兴智华中科技大学管理学院湖北武汉430074

培育股份制商业银行核心竞争力的策略:一是高层策略;二是集中策略;三是优势策略。培育股份制商业银行核心竞争力的途径:一是以壮大整体实力,构造现代企业制度,树立良好的企业形象为基础,培育股份制商业银行的核心竞争力;二是以规范管理... 详细信息

培育股份制商业银行核心竞争力的策略:一是高层策略;二是集中策略;三是优势策略。培育股份制商业银行核心竞争力的途径:一是以壮大整体实力,构造现代企业制度,树立良好的企业形象为基础,培育股份制商业银行的核心竞争力;二是以规范管理为主题,强化内控制约,实行依法治行,提高风险防范能力和管理水平;三是以人力资源开发与合理配置为重点,构建科学的用人机制;四是以市场为导向,不断进行业务创新,打造独具魅力的特色品牌。

关键词：商业银行核心竞争力策略途径

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