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CEA and AFP expression in human hepatoma cells transfected with antisense IGF-I gene

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World Journal of Gastroenterology 1998年第1期4卷 35-37页

作者： ZHANG li li Shu-nong WANG Xiao-Ning Department of Immunology First Military Medical University Guangzhou 510515 Guangdong Province China Department of Pathophysiology Sun Yat-Sen Univ. of Med. Sciences Guangzhou 510089 Guangdong Province China Department of Pathophysiology Sun Yat-Sen Univ. of Med. Sciences China

IM To determine whether antisense insulinlike growth factorI(IGFI) gene can modulate CEA and AFP expression in human hepatoma cells (HepG2).METHODS Transfection of HepG2 cells was accomplished using lipofectin reagent. Northern blot analysis confirmed the antisense IGFI RNA of the transfected cells. CEA and AFP levels were measured using radioimmunoassay.RESULTS Human hepatoma cell lines (HepG2) were transfected with antisense IGFI gene. Northern blot analysis confirmed that antisense IGFI RNA was expressed in the transfected cells. The effect of antisense IGFI gene on CEA and AFP expression was demonstrated by the fact that the CEA and AFP levels in the supernatant of transfected cell culture were significantly lower as compared with the parent cells, 〔CEA 70μg/L±076μg/L and 329μg/L±180μg/L (Plignant potentiality of the transfected cells was partially suppressed. Antisense IGFI gene can modulate the expression of CEA and AFP in human hepatoma cell lines (HepG2).

关键词： insulinlike growth factor-I carcinoembryonic antigen αfetoprotein carcinoma,hepatocellular tumor cells,cultured radioimmunoassay

Synthesis and Crystal Structure of 2-Hydroxybenzoic Acid [1-(3,5-Dibromo-2-hydroxyphenyl)methylidene]hydrazide Methanol

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Chinese Journal of Structural Chemistry 2007年第5期26卷 547-550页

作者： WANG nong li Jing-Ping PU Yan-ling School of Chemical and Biological Engineering Lanzhou Jiaotong University Lanzhou 730070 China

A new Schiff base compound, 2-hydroxybenzoic acid [1-（3,5-dibromo-2-hydroxyphenyl） methylidene]hydrazide methanol （C14H10Br2N2O3·CH3OH）, has been synthesized by the condensation of equimolar 3,5-dibromosalicylaldehyde and 2-hydroxybenzoic acid hydrazide in a methanol solution. The compound was characterized by elemental analysis, IR spectra, and singlecrystal X-ray diffraction. The compound consists of a Schiff base moiety 2-hydroxybenzoic acid [1-（3,5-dibromo-2-hydroxyphenyl）methylidene]hydrazide and a lattice methanol molecule. The crystal belongs to the monoclinic system, space group P21/n with a = 7.183（1）, b = 15.673（2）, c = 15.001（2） A, β = 98.345（2）°, Z = 4, V = 1670.9（4） A^3, Dc = 1.773 g/cm^3, Mr = 446.10, λ（MoKα） = 0.71073 , μ = 4.872 mm^-1, F（000） = 880, R = 0.0458 and wR = 0.0963. A total of 3445 unique reflections were collected, of which 2236 with I 〉 2σ（Ⅰ） were observed. As expected, the molecule adopts a trans configuration about the C=N double bond. The two benzene rings are nearly coplanar （mean deviation from the combined plane is 0.061（4） ）, with the dihedral angle of 7.9（3）°. The preliminary biological tests show that the compound has moderate antibacterial activities.

关键词： Schiff base acylhydrazone crystal structure antibacterial activity

Sensitivity analysis of the De Nitrification and De Composition model for simulating regional carbon budget at the wetlandgrassland area on the Zoige Plateau,China

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Journal of Mountain Science 2016年第7期13卷 1200-1216页

作者： WANG Ji-yan li Ai-nong JIN Hua-an Institute of Mountain Hazards and Environment Chinese Academy of SciencesChengdu 610041China University of Chinese Academy of Sciences Beijing 100049China

Although mathematical models(e.g., De Nitrification and De Composition(DNDC) provide a powerful tool to study regional carbon budget, it is still difficult to obtain accurate simulation results because there exists large uncertainties in modeling regional carbon budget. Through the investigation on the sensitivity of model output parameters to the input parameters, sensitivity analysis(SA) has been proved to be able to identify the key sources of uncertainties and be helpful to reduce the model uncertainties. However, some input parameters with discrete values(e.g., land use type and soil type) and the regional effect of the sensitive parameters were rarely examined in SA. In this paper, taking the Zoige Plateau as a case area, we combined the one-factor-ata-time(OAT) with Extended Fourier Amplitude Sensitivity Test(EFAST) to conduct a SA of DNDC for simulating the regional carbon budget, including Gross Primary Productivity(GPP), Net Primary Productivity(NPP), Net Ecosystem Productivity(NEP), autotrophic respiration(Ra), soil microbial heterotrophic respiration(Rh) and ecosystem respiration(Re). The result showed that the combination of OAT and EFAST could test the contribution of the input parameters with discrete values to the output parameters. In DNDC model, land use type and soil type had a significant impact on the regional carbon budget of the Zoige Plateau, and daily temperature was also confirmed to be one of the most important parameters for carbon budget. For the other input parameters, with the change of land use type or soil type at regional scale, the sensitive parameters of carbon budget would vary accordingly. The SA results would provide scientific evidence to optimize DNDC model and they suggested that we should pay attention to the spatial/temporal effect of SA and try to use the appropriate data in simulation of the regional carbon budget.

关键词： Sensitivity analysis OAT EFAST DNDC model Carbon budget Zoige Plateau

Comparative effects of nitrogen application on growth and nitrogen use in a winter wheat/summer maize rotation system

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Journal of Integrative Agriculture 2017年第9期16卷 2062-2072页

作者： YIN Min-hua li Yuan-nong XU Yuan-bo Key Laboratory of Agricultural Soil and Water Engineering in Arid and Semiarid Areas Ministry of Education/College of Water Resources and Architectural EngineeringNorthwest A&F UniversityYangling 712100P.R.China

The application of fertilizer in agricultural production has become universally common for achieving high crop yields and economic benefits, but it has potential impacts on food safety, energy crisis and environmental pollution. Optimal management of fertilization is thus necessary for maintaining sustainable agriculture. Two-year（2013–2015） field experiment was conducted, in Yangling（108°24′E, 34°20′N, and 521 m a.s.l.）, Shaanxi Province, China, to explore the effects of different nitrogen（N） applications on biomass accumulation, crop N uptake, nitrate N（NO_3~–-N） distribution, yield, and N use with a winter wheat/summer maize rotation system. The N applications consisted of conventional urea（U）（at 80（U80）, 160（U160）, and 240（U240） kg N ha^（–1）; 40% applied as a basal fertilizer and 60% top-dressed at jointing stage） and controlled-release urea（CRU）（at 60（C60）, 120（C120）, 180（C180）, and 240（C240） kg N ha~（^（–1））; all applied as a basal fertilizer） with no N application as a control（CK）. The continuous release of N from CRU matched well with the N demands of crop throughout entire growing stages. Soil NO_3~–-N content varied less and peaked shallower in CRU than that in urea treatments. The differences, however, were smaller in winter wheat than that in summer maize seasons. The average yield of summer maize was the highest in C120 in CRU treatments and in U160 in urea treatments, and apparent N use efficiency（NUE） and N agronomic efficiency（NAE） were higher in C120 than in U160 by averages of 22.67 and 41.91%, respectively. The average yield of winter wheat was the highest in C180 in CRU treatments and in U240 in urea treatments with C180 increasing NUE and NAE by averages of 14.89 and 35.62% over U240, respectively. The annual yields under the two N fertilizers were the highest in C120 and U160. The results suggested that CRU as a basal fertilizer once could be a promising alternative of urea as split application in semiarid areas.

关键词： controlled-release urea nitrogen availability soil fertility nitrogen use efficiency soil productivity

A bibliometric analysis of gene editing research

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High Technology Letters 2020年第1期26卷 118-124页

作者： Yuan Fang Sun Jingsong Zheng Jia li nong Institute of Scientific and Technical Information of China Beijing 100038P.R.China

Gene editing, as a breakthrough in life science and medicine, makes significant scientific impact. The entire development process of gene editing through bibliometric analysis is studied to explore the distribution features and research focus with visual tool. The number of annual publications distribution, journal distribution and country distribution are analyzed in the basic distribution analysis. The keywords and co-citation are analyzed with CiteSpace to explore the research hotspots. The number of publications related to gene editing is increasing year by year. USA is the main publishing countries for gene editing research. CRISPR/Cas system is a hot topic in the field of genetic editing in last few years. Gene editing is opening up a world of possibilities for the treatment of genetic diseases.

关键词： gene editing bibliometric analysis CiteSpace publication

De-stressed mining of multi-seams:Surrounding rock control during the mining of a roadway in the overlying protected seam

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Mining Science and Technology 2011年第2期21卷 159-164页

作者： Wang Cheng Zhang nong li Guichen Zhang Nianchao School of Mines State Key Laboratory of Coal Resources and Safe Mining China University of Mining ＆ Technology Xuzhou 221008 China

Surrounding rock control in the overlying protective coal seam is a challenging topic for de-stressed mining of multi-seamed *** research findings on roadway control were used in the design of a physical model of a complex textured roof having a varying *** model was used to study roadway instability and collapse caused by dynamic *** results show that when the thickness of the roof exceeds the bolted depth the roadway security is least and the roof has the greatest possibility for *** simulations were also carried out to study stress redistribution before and after roadway excavation during underlying protective seam *** evolution of roadway displacement and fracture,as affected by support methods,has been well studied.A series of support principles and technologies for mining affected roadways has been proposed after demonstration of successful practical application in the Huainan *** principles and technologies are of extended value to deep coal mining support in China.

关键词： Multi-seams De-stressed mining Mining influenced roadway Control technology

Consensus on glycemic management for patients with coronary heart disease and type 2 diabetes

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Journal of Geriatric Cardiology 2024年第7期21卷 689-702页

作者： li-nong JI Yun-Dai CHEN Jing liU Xiang-Hai ZHOU Cardiovascular Subcommittee of Chinese International Exchange and Promotion Association for Medical and Healthcare Peking University People's Hospital Chinese PLA General Hospital

The prevalence of patients with coronary heart disease(CHD)and diabetes mellitus is notably high,posing sig-nificant residual cardiovascular risks even after routine interventions such as antihypertensive,lipid-lowering,and antithrombot-ic *** studies have demonstrated that certain glucose-lowering medications confer cardiovascular benefits for pa-tients with type 2 ***,a survey indicates that cardiologists may not be fully acquainted with the optimal screen-ing timing,indicators,and diagnostic criteria for type 2 diabetes,and there is insufficient awareness and a low rate of prescrip-tion of novel glucose-lowering medications with proven cardiovascular efficacy,such as glucagon-like peptide-1 receptor agon-ists(GLP-1 RAs)and sodium-glucose co-transporter-2 inhibitors(SGLT-2i).In this context,based on domestic and international guidelines or consensus and the latest evidence-based evidence,this consensus aims to standardize the glycemic management for patients with acute coronary syndrome,chronic coronary syndrome,and perioperative management for percutaneous coronary *** highlights the key points of screening and diagnosis of type 2 diabetes,and the comprehensive management of cardiovascular risk in patients with *** consensus elaborates on the principles and algorithms of glycemic management for CHD patients,without involving acute complications of diabetes,clarifies the clinical practice of glucose-lowering medications with cardiovascular benefits,and promotes the standardized use of these medications in cardiovascular and other related spe-cialty ***,it addresses the glucose-lowering treatment to comprehensively reduce cardiovascular risks.

关键词： coronary patients routine

Response of Lakes to Climate Change in Xainza Basin Tibetan Plateau Using Multi-Mission Satellite Data from 1976 to 2008

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Journal of Mountain Science 2015年第3期12卷 604-613页

作者： YI Gui-hua DENG Wei li Ai-nong ZHANG Ting-bin Institute of Mountain Hazards and Environment Chinese Academy of Science College of Administrative Science Chengdu University of Technology College of Earth Sciences Chengdu University of Technology Key Laboratory of Geoscience Spatial Information Technology Ministry of Land and Resources of the P.R. China

Changes in the lake areas of Xainza basin in the past 33 years （1976 to 2008） were studied using Landsat data from Multispectral Scanners （1973- 1977）, Thematic Mapper （1989-1992, 2007-2009）, and Enhanced Thematic Mapper Plus （1999-2002）. The results indicated that lakes in the study area evidently expanded from 1976 to 2008, with total expansion of 1512.64km2. The mean annual air temperature presented an upward trend with certain fluctuations from 1966 to 2008. The air temperature rise rates in the cold season （o.31~C/loa） were higher than those in the hot season （0.24℃/1oa）, in the Xainza station example. Precipitation exhibited evident seasonal differences. Mean annual precipitation in hot season is 281.48 mm and cold season is 32.66 mm from 1966 to 2008 in study area. Precipitation in the hot season was the major contributor to the increase in annual precipitation. Grey relational analysis （GRA） was used to study the response of lake areas to climatic factors. The mean air temperature and precipitation were selected as comoared series, and the lake areas were regarded as the reference series. The grey relational grade （GRG） between compared series and reference series were calculated through GRA. The results indicated that changes in lake areas were mainly affected by climatic factors in the hot season. Lakes in this region were classified into three grades, namely, Grades I, II, and III according to the recharge source and elevation. The GRGs of each series varied for different grade lakes： the area of Grade III lakes were the most relevant to the hot season factors, the GRGs of precipitation and air temperature were 0.7570 and 0.6606; followed by the Grade II lakes; Grade I lakes were more sensitive to the air temperature.

关键词： Lake Climate change Remote sensing Grey relational analysis Xainza basin TibetanPlateau

THE METHOD OF THE RECIPROCAL THEOREM OF FORCED VIBRATION FOR THE ELASTIC THIN RECTANGULAR PLATES(Ⅲ)--CANTILEVER RECTANGULAR PLATES

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Applied Mathematics and Mechanics(English Edition) 1991年第7期12卷 663-680页

作者： Fu Bao-lian li nong Yanshan University Qinhuagdao Luoyang Technology Institute Luoyang

In this paper,applying the method of the reciprocal theorem,we give the stationary solutions of the forced vibration of cantilever rectangular plates under uniformly distributed harmonic load and concentrated harmonic load acting at any point of the plates,the figures and tables of number value of bending moment and the deflection amplitudes as well.

关键词： the method of reciprocal theorem forced vibration cantilever rectangular plate the amplitude of bending moment the amplitude of deflection