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Chinese Journal of Chemistry 2024年第1期42卷 61-66页

作者： Huan Wang Shuwen Guo Qiong Yuan meiqi li Yanli Tang Country Key Laboratory of Analytical Chemistry for life Science of Shaanxi Province Key Laboratory of Applied Surface and Colloid ChemistryMinistry of EducationSchool of Chemistry and Chemical EngineeringShaanxi Normal UniversityXi'anShaanxi 710119China

Recently,photodynamic therapy(PDT)has attracted wide attention due to its less susceptibility to drug resistance,broad-spectrum biocidal activity and biosafety in normal ***,the traditional photosensitizers(Ps)face the disadvantage of poor therapeutic efficacy due to the requirement of an aerobic environment to generate ^(1)O_(2) through Type Ⅱ ***,we designed and synthesized a novel cationic conjugated oligomer oligo(phenylene vinylene)(OPV)and studied its antibacterial photodynamic activity against both Gram-negative Escherichia coli(***)and Gram-positive bacteria methicillin-resistant Staphylococcus aureus(MRSA).Importantly,the OpV can rapidly produce reactive oxygen species(ROs)through double pathways,Type Ⅰ and Ⅱ mechanism under white light irradiation,and efficiently kill *** and MRSA at a nanomolar *** dual type photosensitizing capability makes OPV promising for enhanced PDT to treat pathogens and tumors in complex environments.

关键词： Conjugated oligomers Antibacterial photodynamic therapy Oligo(phenylene vinylene) Reactive oxygen species Typelandll pathways

Advanced Algorithm for Parameters Estimation of Negative Binomial Distribution with High Dimensional Sparse Group Structure

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Journal of Systems Science & Complexity 2024年第5期37卷 2173-2195页

作者： li meiqi JIN Baisuo School of Management University of Science and Technology of China

Negative binomial regression is a powerful technique for modeling count data, particularly when dealing with overdispersion. However, estimating the parameters for large-dimensional sparse models is challenging due to the complexity of optimizing the mean and dispersion parameter of the negative binomial distribution. To address this issue, the authors propose a novel approach that employs two iterations of the majorize-minimize(MM) algorithm, one for estimating the dispersion parameter and the other for estimating the mean parameters. These approaches improve the convergence speed and stability of the algorithm. The authors also use group penalty for variable selection, which enhances the accuracy and efficiency of the algorithm. The proposed method provides an explicit solution,simplifies the iteration process, and maintains good stability while ensuring algorithm ***, the authors apply the proposed algorithm to the zero-inflated model and demonstrate its promising predictive performance on specific data sets. The research has important implications for count data modeling and analysis in various fields, such as data mining, machine learning, and bioinformatics.

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Symmetry dependent activation and reactivity of peroxysulfates on FeS_(2)(001)surface

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Science Bulletin 2024年第2期69卷 154-158页

作者： Cancan ling meiqi li Hao li Xiufan liu Guangming Zhan Hongwei Sun Chuan liang Yaling li Bing Zhou Jincai Zhao lizhi Zhang School of Environmental Science and Engineering Shanghai Jiao Tong UniversityShanghai 200240China Key Laboratory of Pesticide&Chemical Biology of Ministry of Education Institute of Environmental&Applied ChemistryCentral China Normal UniversityWuhan 430079China

Heterogeneous peroxysulfates-based advanced oxidation processes(AOPs)have garnered significant attention for purifying organic wastewater since they offer many advantages like low cost,safe storage,facile activation and reactive species participation[1].Nonradical and radical pathways have been proposed to be involved in peroxysulfates activation.

关键词： activation radical reactivity

Regulating Signal Pathway Triggers Circular Reactive Oxygen Species Production to Augment Oxidative Stress with Enzyme-Activated Nanoparticles

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CCS Chemistry 2024年第3期6卷 693-708页

作者： Benkai Bao Qiong Yuan Qian Feng ling li meiqi li Yanli Tang Key Laboratory of Analytical Chemistry for life Science of Shaanxi Province Key Laboratory of Applied Surface and Colloid ChemistryMinistry of EducationSchool of Chemistry and Chemical EngineeringShaanxi Normal UniversityXi’anShaanxi Province 710119

Regulating antioxidative stress pathways to augment oxidative stress and enhance antitumor therapy is highly desirable but very ***,we initiated a multifunctional nanoparticle to regulate the Keap1-Nrf2 antioxidative stress pathway to promote cancer cell *** OPFV-SnMP@GE11 nanoparticles were assembled by enzyme-activated OPFV-TLQ,tin mesoporphyrin(SnMP),and ***-SnMP@GE11 accumulated at tumor sites through specific targeting with ***-TLQ was specifically reduced to a photosensitizer OPFVNH2 by endocellular NAD(P)H:quinone oxidoreductase 1(NQO1).Under irradiation,OPFV-NH2 greatly produced reactive oxygen species(ROS)through a type I mechanism,which activated the Keap1-Nrf2 signal pathway and enhanced the transcription of NQO1,resulting in a continuous and explosive generation of ***,SnMP inhibited the activity of heme oxygenase-1(HO-1),further depressing antioxidative *** strategy provides insight into the regulation of the signal pathway to amplify oxidative stress,paving the way to studying the molecular mechanisms of cellular activities to enhance cancer therapy.

关键词： Keap1-Nrf2 pathway enzyme-activated probe reactive oxygen species oxidative stress antitumor therapy

Ultra-high spatio-temporal resolution imaging with parallel acquisition-readout structured illumination microscopy(PAR-SIM)

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light(Science & Applications) 2024年第6期13卷 1140-1158页

作者： Xinzhu Xu Wenyi Wang liang Qiao Yunzhe Fu Xichuan Ge Kun Zhao Karl Zhanghao Meiling Guan Xin Chen meiqi li Dayong Jin Peng Xi Department of Biomedical Engineering College of Future TechnologyPeking UniversityBeijing100871China Wallace H.Coulter Dept.of Biomedical Engineering Georgia Institute of Technology and Emory UniversityAtlanta30332GAUSA National Biomedical Imaging Center Peking UniversityBeijing100871China Department of Biomedical Engineering College of EngineeringSouthern University of Science and TechnologyShenzhen518055GuangdongChina Airy Technologies Co. Ltd.Beijing100086China Eastern Institute for Advanced Study Eastern Institute of TechnologyNingboZhejiang315200China School of life Science Peking UniversityBeijing100871China Institute for Biomedical Materials and Devices(IBMD) Faculty of ScienceUniversity of Technology SydneySydneyNSW2007Australia

Structured illumination microscopy(SIM)has emerged as a promising super-resolution fluorescence imaging technique,offering diverse configurations and computational strategies to mitigate phototoxicity during real-time imaging of biological *** efforts to enhance system frame rates have concentrated on processing algorithms,like rolling reconstruction or reduced frame reconstruction,or on investments in costly sCMOS cameras with accelerated row readout *** this article,we introduce an approach to elevate SIM frame rates and region of interest(ROI)coverage at the hardware level,without necessitating an upsurge in camera expenses or intricate ***,parallel acquisition-readout SIM(PAR-SIM)achieves the highest imaging speed for fluorescence imaging at currently available detector *** using the full frame-width of the detector through synchronizing the pattern generation and image exposure-readout process,we have achieved a fundamentally stupendous information spatial-temporal flux of 132.9 MPixels·s^(−1),9.6-fold that of the latest techniques,with the lowest SNR of−2.11 dB and 100 nm ***-SIM demonstrates its proficiency in successfully reconstructing diverse cellular organelles in dual excitations,even under conditions of low signal due to ultra-short exposure ***,mitochondrial dynamic tubulation and ongoing membrane fusion processes have been captured in live COS-7 cell,recorded with PAR-SIM at an impressive 408 *** posit that this novel parallel exposure-readout mode not only augments SIM pattern modulation for superior frame rates but also holds the potential to benefit other complex imaging systems with a strategic controlling approach.

关键词： hardware illumination resolution

现代性的内生路径与地方性的询唤可能--从潮汕书写重审“新南方写作”现象

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上海文化 2024年第2期 39-47页

作者：李玫琦南京大学中国新文学研究中心

新世纪的潮汕书写可以反哺“新南方写作”的理论建构。这些作品展现了中国海洋文明的精神影响,显示了启蒙与审美两种现代性的内生可能,同时表明潮语写作并非呈现新南方美学的最优装置,而剖析语言活动肌理、汲取潮汕讲古神韵,更能激发方... 详细信息

新世纪的潮汕书写可以反哺“新南方写作”的理论建构。这些作品展现了中国海洋文明的精神影响,显示了启蒙与审美两种现代性的内生可能,同时表明潮语写作并非呈现新南方美学的最优装置,而剖析语言活动肌理、汲取潮汕讲古神韵,更能激发方言与国语间的艺术张力。对北人南迁的潮汕平原的“无根”状态之考察,则消解了“新南方写作”重构南北方文明序列的意图,揭示了全球化、城市化进程中的普遍精神困境与赛博朋克的乡村现实。这些潮汕书写以现代性与地方性为叙事之双翼,有效规避了“新南方学”理论赋魅的共时本质论风险。

关键词：潮汕书写新南方写作现代性地方性

弓形虫MIC1蛋白在杆状病毒系统中的表达及活性分析

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华中农业大学学报 2024年第1期43卷 210-218页

作者：李响张小涵李美琪陈冉冯颖李林桑晓宇杨娜沈阳农业大学动物科学与医学学院/重要家畜疫病研究教育部重点实验室沈阳110866 辽宁成大生物股份有限公司沈阳110179

为构建可溶性表达弓形虫MIC1蛋白质的重组杆状病毒株并分析重组蛋白质的活性,通过PCR扩增弓形虫mic1基因的编码序列并连接到pFastBac 1质粒中,将重组的转移质粒转化DH10Bac感受态细胞,通过蓝白斑筛选获得重组杆粒,转染Sf9细胞后连续培养... 详细信息

为构建可溶性表达弓形虫MIC1蛋白质的重组杆状病毒株并分析重组蛋白质的活性,通过PCR扩增弓形虫mic1基因的编码序列并连接到pFastBac 1质粒中,将重组的转移质粒转化DH10Bac感受态细胞,通过蓝白斑筛选获得重组杆粒,转染Sf9细胞后连续培养3代获得重组杆状病毒株。结果显示,Sf9细胞在感染后的第3天出现典型的细胞病变;间接免疫荧光和Western blot试验结果表明MIC1重组蛋白质在感染的Sf9细胞中成功可溶性表达;纯化的MIC1重组蛋白质不仅具有结合唾液酸乳糖的能力,同时能够刺激Balb/c小鼠产生较高水平的特异性抗体(>1∶25600)。以上结果表明,通过杆状病毒表达系统可获得具有生物学活性的弓形虫MIC1重组蛋白质。

关键词：弓形虫 MIC1蛋白杆状病毒系统活性分析

农业强国战略背景下中国大豆供给安全:现实基础、潜力研判与策略选择

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农业经济问题 2024年第7期 48-58页

作者：李辉尚李美琪黄晓慧胡冰川中国农业科学院农业信息研究所北京100081 中国社会科学院农村发展研究所北京100732

大豆是重要的战略性农产品之一,在农业强国战略中有着突出重要地位。但我国大豆对外依存度和进口来源集中度过高,在全球地缘政治等重大事件冲击下,贸易链、供应链不确定性风险集聚、脆弱性凸显;立足国内,确保大豆供给安全,实现风险可控... 详细信息

大豆是重要的战略性农产品之一,在农业强国战略中有着突出重要地位。但我国大豆对外依存度和进口来源集中度过高,在全球地缘政治等重大事件冲击下,贸易链、供应链不确定性风险集聚、脆弱性凸显;立足国内,确保大豆供给安全,实现风险可控,是我国新时期全面保障粮食安全的战略需要,也是实现农业强国建设目标的内在要求。当前,我国大豆有效供给面临技术“卡脖子”问题突出、核心产区加速北移、比较效益相对较低和供需结构性矛盾凸显等“四元”现实困境;多情形测算结果显示,立足国内提升大豆产能的潜力巨大,需多措并举、综合施策,持续增强我国大豆供给安全保障能力。

关键词：农业强国大豆供给安全潜力研判策略路径

果蔬逆境胁迫应答中AP2/ERF转录因子的激素调控研究进展

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食品科学 2024年第12期45卷 315-323页

作者：李皎琪李美琪王玉婷刘秀玲张新华李富军李晓安山东理工大学农业工程与食品科学学院山东淄博255049

APETALA2/乙烯响应因子(APETALA2/ethylene-responsive factor,AP2/ERF)转录因子家族是植物中最大的转录因子家族之一,其亚家族成员通过与下游互作基因启动子区域的GCC box、DRE/CRT等顺式作用元件特异性结合,激活或抑制相关基因的表达... 详细信息

APETALA2/乙烯响应因子(APETALA2/ethylene-responsive factor,AP2/ERF)转录因子家族是植物中最大的转录因子家族之一,其亚家族成员通过与下游互作基因启动子区域的GCC box、DRE/CRT等顺式作用元件特异性结合,激活或抑制相关基因的表达,参与果蔬的生长发育、成熟衰老、胁迫应答等生物学过程的调控。近年来越来越多的研究表明,AP2/ERF还可通过调控靶基因诱导乙烯、茉莉酸、水杨酸等多种植物激素反应或作为反应因子响应植物激素信号,参与植物激素介导的果蔬品质形成、生物胁迫及非生物胁迫应答相关的代谢通路,调节果蔬的品质及抗性。本文对AP2/ERF转录因子的结构特征及其在果蔬中的分类和分布进行了概述,并综述了果蔬逆境胁迫应答中AP2/ERF与主要激素信号相互作用以调控果蔬抗逆性的最新研究进展,以期从AP2/ERF转录因子激素调控的角度,为增强果蔬对逆境胁迫的抗性提供理论依据。

关键词： AP2/ERF转录因子植物激素果蔬生物胁迫非生物胁迫

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浅谈信息化建设在高校教务管理系统的应用

教育教学研究前沿

教育教学研究前沿 2024年第1期2卷 91-94页

作者：李美琪张婷西安工商学院陕西西安710200

随着信息技术的发展和普及,信息化建设已成为各行各业的重要发展方向。高校作为培养人才和推动社会进步的重要机构,其教务管理系统的信息化建设显得尤为重要。在高校教务管理系统中应用信息化建设,能够提高教务管理的效率和服务质量,实... 详细信息

随着信息技术的发展和普及,信息化建设已成为各行各业的重要发展方向。高校作为培养人才和推动社会进步的重要机构,其教务管理系统的信息化建设显得尤为重要。在高校教务管理系统中应用信息化建设,能够提高教务管理的效率和服务质量,实现教学资源的优化配置,促进教学改革和教学质量的提升。因而研究信息化建设在高校教务管理系统的应用有重要意义。对此,本文首先阐述信息化建设在高校教务管理系统的价值意蕴,进而探究信息化建设在高校教务管理系统的应用,以期推动高校教育教学工作的信息化发展。

关键词：信息化建设高校教务管理系统