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Analysis of the diversity and function of the alleles of the rice blast resistance genes Piz-t, Pita and Pik in 24 rice cultivars

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Journal of Integrative Agriculture 2016年第7期15卷 1423-1431页

作者： WANG yan ZHAO Jia-ming ZHANG li-xia WANG Ping WANG Shi-wei WANG Hui WANG Xiao-xi liU Zhi-heng ZHENG Wen-jing Plant Protection College Shenyang Agricultural University Agricultural Crops Molecular Improving Laboratory Liaoning Academy of Agricultural Sciences Department of Plant Pathology China Agricultural University

Understanding the sequence diversity of rice blast resistance genes is important for breeding new resistant rice cultivars against the rice blast fungusMagnaporthe oryzae. In this study, we selected 24 rice cultivars with different genetic back-grounds to study the alelic diversity of rice blast resistance genesPiz-t, Pitaand Pik. For Piz-t, a total of 17 alelic types were found within the 24 cultivars. Blast inoculations showed that most of the mutations can affect the function of the resistance gene. For Pita, except for the difference at the 918th amino acid, a majority of the 21 mutations were detected among the cultivars. Inoculations with blast isolates carryingAvr-Pita revealed that cultivars with mutations in other sites except for the 918th amino acid did not affect the function of thePita gene. ForPik, a total of six alelic types were found within the 24 cultivars, but ifve of them lost the function of the resistance gene. In addition, we found thatPiz-t, Pita and Pik were expressed constitutively in the 24 rice cultivars and the expression level was not related to resistance. Our results have provided the sequence diversity information of the resistance genesPiz-t, Pita and Pik among the popular rice cultivars grown in the northeast region of China. Keywords：resistance gene, avirulence gene, aleles, function, genetic evolution zae（M. oryzae）, is one of the most destructive diseases in rice production worldwide. Over the years, comprehensive studies on rice blast resistance have been conducted （Silue et al. 1992）. The resistance in newly cultivated rice cultivars to M. oryzae can be lost quickly due to the high level of instability in the genome of the fungus （Bonmanet al. 1992）. Previous studies show that cultivars with durable and broad-spectrum resistance againstM. oryzae carry multiple major resistance （R） and minor resistance genes （liuet al. 2014）. An effective way to control rice blast disease is, therefore, to breed rice cultivars with multiple R

关键词： resistance gene avirulence gene aleles function genetic evolution

Porcine methionine sulfoxide reductase B3:molecular cloning,tissue-specific expression profiles,and polymorphisms associated with ear size in Sus scrofa

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Journal of Animal Science and Biotechnology 2016年第2期7卷 149-157页

作者： Yuebo Zhang jing liang Longchao Zhang ligang Wang Xin liu Hua yan Kebin Zhao Huibi Shi Tian Zhang Na li Lei Pu lixian Wang Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation Ministry of AgricultureInstitute of Animal ScienceChinese Academy of Agricultural SciencesBeijing 100193China Jilin Academy of Agricultural Sciences Changchun 130033China

Background： In Sus scrofa, methionine sulfoxide reductase B3（MSRB3） is a crucial candidate gene for ear size, and an important conformational trait of pig breeds. However, challenges in MSRB3 c DNA amplification have prevented further identification of MSRB3 allelic variants influencing pig ear ***： We cloned a full-length c DNA sequence of porcine MSRB3 by rapid-amplification of c DNA ends. The3,765-bp gene contained a 5＇-untranslated region（UTR）（190 bp）, a coding region（552 bp）, and a 3＇-UTR（3,016 bp） and shared 84 %, 84 %, 87 %, 86 %, and 70 % sequence identities with human, orangutan, mouse, chicken, and zebrafish,respectively. The gene encoded a 183-amino acid protein, which shared 88 %, 91 %, 89 %, 86 %, and 67 % identities with human, orangutan, mouse, chicken, and zebrafish, respectively. Tissue expression analysis using q RT-PCR revealed that MSRB3 was expressed in the heart, liver, lung, kidney, spleen, ear, muscle, fat, lymph, skeletal, and hypothalamic tissues. Three single nucleotide polymorphisms（SNPs） were identified in MSRB3： c.-735 C 〉 T in the 5＇ flanking region,c.2571 T 〉 C in the 3＇-UTR, and a synonymous mutation of c.484 T 〉 C in the coding region. The SNPs c.-735 C 〉 T and c.2571 T 〉 C were significantly associated with ear size in a Large White × Minzhu F2 population other than in Beijing Black pigs. Subsequently, at SNP c.-735 C 〉 T, the m RNA of MSRB3 was significantly higher expressed in ears of individuals with the TT genotype（Minzhu） than those with CC（Large White）.Conclusions： The porcine MSRB3 owned a 3,765-bp full-length c DNA sequence and was detected to express in ear tissue. Two SNPs of this gene were shown to be significantly associated with ear size in a Large White × Minzhu intercross population instead of Beijing Black pig population. What＇s more, the individuals with higher m RNA expression of MSRB3 have larger ear sizes. These results provide useful information for further functional analyses of MSRB3 influencing ear

关键词： Association study Ear size Expression MSRB3 cloning Pig

Glucuronidation of Two Novel Metabolites of Benproperine with Trichloroacetimidate Donor

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Chemical Research in Chinese Universities 2006年第4期22卷 455-458页

作者： li yan HARA Osamu MAEBA Isamu ZHONG Da-fang School of Pharmacy School of Pharmaceutical Engineering Shenyang Pharmaceutical UniversityShenyang 110016 P. R. China Faculty of Pharmacy Meijo University Nagoya 460-8503 Japan

The glucuronide metabolites of benproperine were synthesized from mono-hydroxylate metabolites of benproperine that were treated with methyl （2,3,4-tri-O-acetyl-1-O-tfichloroacetimidoyl ） -α-D-glucopyranuronate with BF3 · Eh O as the promoter followed by basic hydrolyzation with Na2 CO3. The form of basic acceptors, the order of addition, and the promoter are all important variables in this glucuronidation. The salt form of the basic acceptor was found to be better than its free form for glucuronidation with a Lewis acid as the promoter. Two mono-hydroxylated benproperines were synthesized from 2-benzylphenol in three steps.

关键词： Glucuronidation Benproperine Metabolite Trichloroacetimidate

Microbial diversity in the sediments of the southern Mariana Trench

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Journal of Oceanology and limnology 2019年第3期37卷 1024-1029页

作者： li Yingjie CAO Wenrui WANG yan MA Qingjun Key Laboratory of Experimental Marine Biology Institute of OceanologyChinese Academy of SciencesQingdao 266071China Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdao 266000China Key Laboratory of Marine Geology and Environment Institute of OceanologyChinese Academy of SciencesQingdao 266071China University of Chinese Academy of Sciences Beijing 100049China Center for Ocean Mega-Science Chinese Academy of SciencesQingdao 266071China

Microbial diversity in the abyssal sediments beneath the seafloor of 30,94,and 151cm near the southern end of the Mariana Trench was analyzed in the Illumina HiSeq 2500 *** show that the microbial populations were dominated by bacteria but merely no archaea were identifi ed at the three *** the bacterial community,Proteobacteria and Firmicutes dominated the total taxon tags,followed by Bacteroidetes,Actinobacteria,Planctomycetes,Cyanobacteria,and Chloroflexi,which together account for over 99%of the total *** to that in the seawater in the trench,the operational taxonomic units(OTUs)belonging to Gammaproteobacteria from the sediment samples showed high ***,common bacterial OTUs in the water of the trench including Nitrospirae and Marinimicrobia were hardly found in the sediments from the southern Mariana Trench or the hadal ***,this study documented for the first time the compositions of microbial diversity in the trench sediments,revealed the difference in microbial diversity in water and sediment of the trench and will enrich the knowledge on the microbial diversity in the abyssal areas.

关键词： Mariana Trench microbial diversity high-throughput sequencing technology

A Theoretical Analysis of Ultraslow Optical Solitons via Exciton Spin Coherence in GaAs/AlGaAs Multiple Quantum Wells

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Chinese Physics Letters 2013年第2期30卷 210-213页

作者： yan Wei WANG Tao li Xiao-Ming Wuhan National Laboratory for Optoelectronics Huazhong University of Science and TechnologyWuhan 430074

We show the formation of ultraslow bright and dark optical solitons in aΛ-type three-level system with a GaAs/AlGaAs multiple quantum well(MQW)structure based on the exciton spin *** propagation of the pulse across the quantum wells is studied analytically and numerically with Maxwell-Schr-dinger *** research of ultraslow optical solitons of MQWs in the present work may provide important applications in optical devices and optical communication systems.

关键词： equations. quantum solitons

Preparation, Electrochemical Property and Application in Bulk-modified Electrode of Dawson-type Phospho-molybdate-doped Polypyrrole Composite Nanoparticles

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Chemical Research in Chinese Universities 2006年第5期22卷 556-559页

作者： WANG Xiu-li ZHAO Hai-yan WANG Yi-fei Faculty of Chemistry and Chemical Engineering Bohai University Jinzhou 121000 P. R. China

A kind of inorganic-organic hybrid semiconductor composite nanoparticles： Dawson-type phosphomolybdate- doped polypyrrole （P2Mo18-PPy） was designed and prepared using microemulsion oxidation-polymerization at room temperature and characterized by TEM and IR. The P2Mo18-PPy was used as a bulk-modifier to fabricate a chemically modified carbon paste electrode（CPE） by direct mixing, which represents the example of polyoxometalates（ POMs）- doped semiconductor polymer nanoparticles modified electrode. Both the advantage of POMs-doped polymer and the surface-renewal property of the CPE were fully utilized. The electrochemical behavior of the P2Mo18-PPY bulk-modified CPE（P2Mo18-PPy-CPE） was investigated with cyclic voltammetry. Three couples of reversible redox peaks were observed in the range from ＋ 800 to 0 mV, which corresponded to the reduction and oxidation through two-, four- and six-electron processes, respectively. The P2 Mo18-PPY-CPE showed a high electrocatalytic activity for the reduction of nitrite, which expanded the application of POMs-doped semiconductor polymer nanoparticles.

关键词： Dawson-type phosphomolybdate Polypyrrole Composite nanoparticles Bulk-modified Carbon paste electrode Electrocatalysis

Influence of morphology and hemodynamics on thrombosis in kawasaki disease patients

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Medicine in Novel Technology and Devices 2023年第2期18卷 91-101页

作者： Haoyao Cao Zhuxiang Xiong Zhan liu yan li Huaxia Pu jing liu liqing Peng Tinghui Zheng Department of Mechanics Sichuan UniversityChengdu610065China Sichuan University Yibin Park Yibin Institute of Industrial TechnologyYibin644000China Department of Radiology West China HospitalSichuan UniversityChengdu610041China Med-X Center for Informatics Sichuan UniversityChengdu610041China

Kawasaki Disease(KD)patients with co-occurrent coronary artery aneurysms(CAAs)are prone to *** study explores the thrombotic influence of morphology and hemodynamics in KD patients with ***-specific computed tomography angiogram images of 9 patients were used to rebuild coronary models(including 23 aneurysms;10 in thrombosed group,and 13 in non-thrombosed group)and perform computational simulations to obtain the hemodynamic *** thrombosed and non-thrombosed groups were compared in terms of six parameters,namely,the maximum diameter(D_(max)),aspect ratio(R_(l/d)),shape,time-averaged wall shear stress(TAWSS),oscillatory shear index(OSI),and relative residence time(RRT).The results showed that:(1)In thrombosed aneurysms,there were several vortex structures(eddy zone),of which the position changed continuously in a cardiac *** non-thrombosed aneurysms,the streamlines showed a large streaming zone.(2)Abnormal hemodynamic areas of aneurysms with thrombosis tended to appear in the same region(especially the proximal and near myocardial side of aneurysms).(3)In the non-thrombosed group,there was a correlation between the morphological and hemodynamic *** thrombosed aneurysms,the flow pulsatility has a significant impact on the flow *** thrombosed coronary aneurysms exhibited more risk factors and the co-location of hemodynamic abnormalities was consistent with the position of thrombosis.A score of risk factors could provide the thrombotic information of coronary aneurysms,which might be helpful for long-term clinical management of KD patients.

关键词： Coronary artery aneurysms Kawasaki disease Thrombotic risk stratification Morphology Hemodynamics

Enhanced alumina film adhesion of Hf/Y-doped iron-aluminum alloys during high-temperature oxidation:a new observation

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Rare Metals 2019年第9期38卷 877-884页

作者： Dong-Qing li li-Xian Zhou Jun Zhan jing-Chao Wang Jian Gu Jia-Jun Si Department of Transmission and Transformation Engineering Mechanics China Electric Power Research Institute

The cyclic oxidation behavior of Hf/Y-doped B2 FeAl intermetallics at 1373 K was *** an undoped FeAl alloy,premature spallation of the alumina film occurs due to the formation of numerous voids at the film/alloy interface and apparent shrinkage in the *** contrast to this,doping with either Hf or Y significantly improves the interfacial adhesion between the alumina film and the alloy substrate,particularly with *** observation in combination with Anger electron spectroscopic analysis suggests that in addition to prohibiting interfacial void formation and alleviating film shrinkage,the addition of Hf in the FeAl alloy could consolidate the film/alloy interface by directly participating in chemical bonding across the interface as a Hf *** causes the spallation of alumina film from the equiaxed grains/columnar grains interface rather than the bottom of the film.

关键词： Intermetallics Reactive elements Thermal cycling Alumina film Interfacial adhesion Segregation

Analysis of the spatiotemporal trends and influencing factors of Hyphantria cunea in China

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Journal of Forestry Research 2024年第6期35卷 294-305页

作者： Hongwei Zhou Chaoqun Gong Xiaodong li Yue Wang Yunbo yan College of Computer and Control Engineering Northeast Forestry UniversityHarbin 150040People’s Republic of China Key Laboratory of National Forestry and Grassland Administration On Forest and Grassland Pest Monitoring and Warning Center for Biological Disaster Prevention and ControlNational Forestry and Grassland AdministrationShenyang 110034People’s Republic of China

In recent years,the situation of the Hyphantria cunea(Drury)(Lepidoptera:Erebidae),infestation in China has been serious and has a tendency to continue to spread.A comprehensive analysis was carried out to examine the spa-tial distribution trends and influencing factors of *** analysis involved integrating administrative division and boundary data,distribution data of ***,and envi-ronmental variables for *** and gravity analysis techniques were employed for data processing and *** results show that *** exhibited high aggregation patterns in 2021 and 2022 concentrated mainly in eastern *** these years,the focal point of the infestation was in Shandong Province with a spread towards the *** such as high vegetation density in eastern China provided favorable situations for growth and development of *** China,the spatial distribution of the moth is primarily influenced by two critical factors:precipitation during the driest month and *** play a pivotal role in determining the spread of the *** on these results,suggestions are provided for a mul-tifaceted approach to prevention and control of *** infestation.

关键词： Hyphantria cunea Temporal trends GeoDetector spatial analysis Spatial heterogeneity