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Developing a risk assessment system for gas tunnel disasters in China

Journal of Mountain Science 2017年第9期14卷 1751-1762页

作者： KANG Xiao-bing LUO Sheng li qing-shan XU Mo li Qiang State Key Laboratory of Geohazard Prevention and Geoenvironment Protection Chengdu University of Technology College of Engineering Sichuan Normal University

Gas disasters always restrict the tunnel constructions in mountain area, which becomes a major geological barrier against the development of underground engineering. China is rich in coal resources and has a large amount of gas with a wide range of distribution. However, China experienced not only adverse effects on coal mining but also gas disasters in underground engineering construction, such as tunnels and chambers. With the increased number of tunnels passing through coal-bearing strata, the incidence of gas accidents is also rising. Therefore, the significance of preventing and mitigating gas disasters should be emphasized, and an effective risk assessment method for gas disasters should be established. On the basis of research on over 100 gas tunnels in China, a relatively ideal gas disaster risk assessment method and system for tunnels are established through the following measures. Firstly, geo-environmental conditions and gas situations were analyzed during construction. Secondly, qualitative analysis was combined with quantitative analysis. Finally, the influencing factors of gas disasters, including geological conditions, gas,and human factors, were investigated. The gas tunnel risk assessment system includes three levels:(1) the grading assessment of a gas tunnel during the planning stage,(2) the risk assessment of gas tunnel construction during the design and construction stages,(3) the gas tunnel outburst risk assessment during the coal uncovering stage. This system was applied to the dynamic assessment of gas disaster during the construction of the Zipingpu tunnel of Dujiangyan–Wenchuan Highway(in Sichuan, Southwest China). The assessment results were consistent with the actual excavation, which verified the rationality and feasibility of the system. The developed system was believed to be back-up and applied for risk assessment of gas disaster in the underground engineering construction.

关键词： Tunnel engineering Gas disasters Risk assessment Index system Zipingpu tunnel

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Purification and in vitro Activity of an Antimicrobial Peptide from Skin of Rana Temporaria Chensinensis,David

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Chemical Research in Chinese Universities 2007年第4期23卷 433-436页

作者： ZHOU Jie MENG qing-fan XU Xue-song TIAN Xiao-le JIANG Fu-jia li qing-shan TENG li-rong College of life Science Jilin University Changchun 130012 P. R. China Department of Laboratory China-Japan Union Hospital Jilin University Changchun 130033 P. R.China

In this study, an antimicrobial component（RTCI） was purified from the skin of Rana temporaria chensinensis, David. Antimicrobial activities of RTCI against clinical multi-drug resistant bacterial strains, including Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureaus, Klebsiella oxytoca, Klebsiella pneumoniae, Enterococcus fae-calis, and Proteus mirabilis, were measured in vitro by means of minimal inhibitory concentration and time-kill studies. The results indicate that RTCI could inhibit the growth of these bacteria at a proper concentration and suggest that RT-CI shows a better antimicrobial activity to Gram-negative bacterial strains than to Gram-positive bacterial strains.

关键词： Antimicrobial peptides Multi-drug resistant Amphibian skin

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Synthesis and activities of new 4-hydroxybenzoxazolone derivatives

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Chinese Chemical Letters 2010年第1期21卷 63-66页

作者： Tang, li Ban, Shu Rong Feng, Xiu E. lin, Wen Han li, qing shan shanxi Med Univ Sch Pharmaceut Sci Taiyuan 030001 Peoples R China Peking Univ State Key Lab Nat & Biomimet Drugs Beijing 100083 Peoples R China

A series of new 4-substituted benzoxazolone derivatives were synthesized according to a convenient method,their anti-inflammatory and analgesic activities in vivo were *** of them were new compounds,the structures of all the synthesized compounds were confirmed by ~1H NMR,^(13)C NMR,ESI-MS and *** of the compounds exhibited anti-inflammatory activity.

关键词： Synthesis 4-Hydroxy benzoxazolone derivatives Anti-inflammatory activity

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Synthesis and Anti-VSMC Vegetation Activities of New Aurone Derivatives

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Chemical Research in Chinese Universities 2012年第3期28卷 438-442页

作者： FAN Rui BAN Shu-rong FENG Xiu-e ZHAO Chen-xiao li qing-shan School of Pharmaceutical Science Shanxi Medical UniversityTaiyuan 030001P.R.China State Key Laboratory of Natural and Biomimetic Drugs Peking UniversityBeijing 100083P.R.China

A series of new aurone derivatives was prepared by means of a practical route and their anti-vascular smooth muscle cells（VSMC） vegetation activities were evaluated by the 3-（4,5-dimethylthlazol-2-yl）-2,5- diphenyltetrazolium bromide（MTT） method with tetrandrine as a positive contrast drug. The structures of the com- pounds were confirmed by 1H NMR, 13C NMR and electrospray ionization mass spectrometry（ESI-MS）. Several new compounds exhibited promising activity against VSMC proliferation and the preliminary structure-activity relation- ships（SAR） were discussed in order to investigate the essential structures required for their bioactivities.

关键词： Aurone derivative Anti-vascular smooth muscle cell(VSMC) vegetation activity Synthesis

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FEM Analysis of Rolling Pressure Along Strip Width in Cold Rolling Process

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Journal of Iron and Steel Research International 2007年第5期14卷 22-26页

作者： liU Xiang-hua SHI Xu li shan-qing XU Jian-yong WANG Guo-dong State Key Laboratory of Rolling and Automation Northeastern University Shenyang 110004 Liaoning China Research and Development Center Baoshan Iron and Steel Co Ltd Shanghai 201900 China Baosteel Branch Co Baoshan Iron and Steel Co Ltd Shanghai 201900 China

Using 3-D elastic-plastic FEM, the cold strip rolling process in a 4-high mill was simulated. The elastic deformation of rolls, the plastic deformation of the strip, and the pressure between the work roll and the backup roll were taken into account. The distribution of rolling pressure along the strip width was obtained. Based on the simulation results, the peak value of rolling pressure and the location of the peak were analyzed under different rolling conditions. The effects of the roll bending force and the strip width on the distribution of rolling pressure along the width direction were determined.

关键词： thin strip cold rolling elastic-plastic FEM pressure distribution

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Prokaryotic Expression and Polyclonal Antibody Preparation of PRL3

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Chemical Research in Chinese Universities 2007年第6期23卷 697-700页

作者： SHEN Xing-gui li Wan-nan WANG Jing JIANG Yi-qun li qing-shan FU Xue-qi Edmond H. Fischer Signal Transduction Laboratory College of Life Sciences Jilin University Changchun 130021 P. R. China

Phosphatase of regenerating liver 3 （ PRL3 ）, which belongs to the superfamily of protein tyrosine phosphatases （PTPs）, represents a group of low molecular weight PTPs that participate in tumorigenesis and metastasis processes. Presented here are the results of cloning, prokaryotic expression, purification, and polyclonal antibody preparation of PRL3. To obtain a specific polyclonal antibody against PRL3, the authors have prepared GST-PRL3 to immunize rabbits and purify an anti-PRL3 polyclonal antibody by negative selection affinity columns. Western blot analysis shows that the anti-PRL3 polyclonal antibody has a specific binding ability with PRL3 protein. The anti-PRL3 polyclonal antibody provides a good tool to further study the function of PRL3.

关键词： PRL3 Prokaryotic expression Polyclonal antibody

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PRLs Promote Spreading, Adhesion, and Proliferation of Human SW480 and SW620 Cells

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Chemical Research in Chinese Universities 2006年第1期22卷 68-72页

作者： li Zhao-fa XU Xue-song SHEN Xing-gui li qing-shan ZHAO Zhi-zhuang FU Xue-qi li Yu-lin li Yi-lei Edmond H. Fischer Signal Transduction Laboratory College of Life Sciences Jilin University Changchun 130021 P. R. China China-Japan Union Hospital Jilin University Changchun 130031 P. R. China The Key Laboratory of Pathobiology Ministry of Education Jilin University Changchun 130023 P. R. China

Recent studies suggest that PRL-3 is involved in the metastasis of colorectal cancer, but the mechanism concerning that has not been well defined. This article expresses PRL-1, PRL-2, and PRL-3 and the catalytically inactive mutant forms of those enzymes in SW620 and SW480 cells, two human cell lines derived from non-metastatic cancer and metastatic colorectal cancer, respectively. While the expression of the native forms of PRLs promotes the spreading, adhesion, and proliferation of these cells, the expression of their mutant forms inhibits the earlier-mentioned processes. These data thus provide a cellular mechanism for the role of PRL-3 in tumor metastasis and suggest that all the three PRLs have similar functions.

关键词： PRL Metastasis Proliferation Expression SW480 cell SW620 cell

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Kinetics of Phosphatase of Regenerating liver-3(PRL-3) Inhibition by Small-molecular Inhibitors

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Chemical Research in Chinese Universities 2006年第2期22卷 221-224页

作者： SHEN Xing-gui SUN li-wen JIAO Ming li Zhao-fa ZHAO Zhi-zhuang li qing-shan FU Xue-qi HUANG Zhong-xiu Edmond H. Fischer Signal Transduction Laboratory College of Life Sciences Jilin University Changchun 130021 P. R. China Qianwei Campus Clinical Hospital of Jilin University Changchun 130021 P. R. China

Phosphatase of Regenerating liver-3 （PRL-3） is a newly identified colorectal cancer metastasis-related protein, which is a 22 kDa non-classical protein tyrosine phosphatase with a C-terminal prenylation motif. In this study, the inhibition kinetics of protein tyrosine phosphatases（PTPs） by a fluorescent substrate, 6,8-difluoro-4-methylumbelliferyl phosphate（DiFMUP） was evaluated. PRL-3 exhibits classical Michaelis-Menten kinetics with a Vmax value of 11.3 μmol · L^-l · min^-1 Analysis of PRL-3 by a Michaelis-Menten plot and a double-reciprocal plot indicated that the inhibitor magnolol can cause Km to increase, but does not alter the Vmax value, which suggests the competitive inhibition of PRL-3. At the same time, it was found that DiFMUP is a more sensitive substrate for PRL-3 than para-nitrophenyl phosphate（pNPP） that is more frequently used at present. Furthermore, the method of screening for PTPs by the use of DiFMUP was developed, which studied the acceptance of DiFMUP by other PTPs.

关键词： PRL-3 DiFMUP Kinetics Inhibitor

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Thermal effects on structure and photoluminescence properties of diamond-like carbon films prepared by pulsed laser deposition

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Optoelectronics Letters 2006年第3期2卷 182-185页

作者： CHEN Da li qing-shan WANG Jing-jing ZHENG Xue-gang College of Physics and Engineering Qufu Normal University Qufu 273165 China

Un-hydrogenated Diamond-like Carbon （DLC） films were prepared by pulsed laser deposition technique at different substrate temperature. The Raman spectra, the absorption and the photoluminescence spectra were measured. The dependence of structure and phetoluminescence properties on deposition temperature were studied in detail. The experimental results indicate that the sp^2 sites form small clus ters that consist of both olefinic chains and aromatic ring groups within the sp^3 matrix. With raising deposition temperature,the optical band gaps increase from 1.87 to 2.85 eV. The main band of photoluminescence centered at around 700nm shifts to short wavelength, and the intensity of this band increases. The photoluminescence can be attributed to carrier localization within an increasing sp^2 clusters. It was clarified that the DLC films are ordered with increasing deposition temperature.

关键词：碳薄膜光致发光金刚石脉冲激光沉积作用光谱

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Synthesis of New Flavanone Derivatives of Farrerol and Preliminary SAR Studies on Anti-VSMCs Vegetation Activity

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Chemical Research in Chinese Universities 2011年第2期27卷 237-240页

作者： SHI Lei FENG Xiu-e liN Wen-han FANG lian-hua DU Guan-hua li qing-shan School of Pharmaceutical Science Shanxi Medical University Taiyuan 030001 P R. China State Key Laboratory of Natural and Biomimetic Drugs Peking University Beijing 100083 P R. China Institute of Materia Medica Chinese Academy of Medical Sciences and Peking Union Medical College Beijing 100050 P R. China

A series of new flavanone derivatives of farrerol was designed and synthesized as a potent inhibitor of vascular smooth muscle cells（VSMCs） vegetation according to a convenient method. The structures of all the synthesized compounds were confirmed by 1H NMR, 13C NMR and EIHR-MS. The biological activities of these compounds against VSMCs in vitro were evaluated. The assay results indicate that two compounds, 5,7-dihydroxy-6,8-dimethyl- 2-（2-nitrophenyl）chroman-4-one（7f） and 2,3-dibromo-4,5-dihydroxydiphenylmethanone（7j） exhibited high activity against VSMCs in vitro with IC50 values of 9.9 and 6.7 μmol/L, respectively, and the preliminary structure-activity relationship（SAR） was described.

关键词： Synthesis Flavanone derivative Anti-VSMCs vegetation

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