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基于RORγt/Foxp3免疫失衡探讨养阴清肺汤加味对支原体感染小鼠免疫微环境的影响

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时珍国医国药 2024年第6期35卷 1333-1336页

作者：吴振起张文涵杨馥语王慧婧武美彤李美琪辽宁中医药大学附属第二医院辽宁沈阳110034 辽宁中医药大学辽宁沈阳110847

目的基于RORγt/Foxp3免疫失衡机制,观察不同剂量养阴清肺汤加味对肺炎支原体(MP)感染小鼠免疫微环境的影响,探讨中药量-效关系对免疫微环境的动态影响。方法将36只BALB/c小鼠随机分为正常组、模型组、阿奇组、养阴清肺汤加味(YQDP)组(... 详细信息

目的基于RORγt/Foxp3免疫失衡机制,观察不同剂量养阴清肺汤加味对肺炎支原体(MP)感染小鼠免疫微环境的影响,探讨中药量-效关系对免疫微环境的动态影响。方法将36只BALB/c小鼠随机分为正常组、模型组、阿奇组、养阴清肺汤加味(YQDP)组(低剂量组、中剂量组及高剂量组),除正常组小鼠外,各组小鼠经麻醉后使用1010 CCU/ml的MP菌液滴鼻构建模型成功后予以药物治疗,于用药干预第7天取材。qPCR法检测肺组织中RORγt、Foxp3 mRNA含量,Western blot法、免疫组化法分析肺组织中RORγt、Foxp3的蛋白表达情况,HPLC法检测小鼠BALF中DPPC含量,EliSA法检测小鼠血清中TGF-β、IL-17A、IL-10及BALF中SP-A、SP-D含量。结果造模肺组织中RORγt表达增加、Foxp3表达降低,血清中TGF-β、IL-17A含量增高,血清与BALF中IL-10、SP-A、SP-D、DPPC含量下降(P<0.05)。治疗后,小鼠RORγt、TGF-β、IL-17A均降低,Foxp3、IL-10、SP-A、SP-D、DPPC均有不同程度的回升(P<0.05)。结论不同剂量的养阴清肺汤加味均在一定程度上可调控RORγt/Foxp3免疫平衡机制及肺表面活性物质SP-A、SP-D、DPPC动态差异表达,从而影响小鼠肺部免疫微环境的动态变化。

关键词：肺炎支原体肺炎养阴清肺汤加味免疫微环境 RORγt/Foxp3 肺表面活性物质

Effect of short neuropeptide F signaling on larval feeding in Mythimna separata

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Insect Science 2024年第2期31卷 417-434页

作者： mei-mei li qi Yang li-Hui Chen Yan-Ying li Jun-Xiang Wu Xiang-li Xu State Key Laboratory of Crop Stress Biology in Arid Areas College of Plant ProtectionNorthwest A&F UniversityYanglingShaanxi ProvinceChinaand School of Agricultural Sciences Jiangxi Agricultural UniversityNanchangChina

Mythimna separata is a notorious phytophagous pest which poses serious threats to cereal crops owing to the gluttony of the *** short neuropeptide F(sNPF)and its receptor sNPFR are involved in a diversity of physiological functions,especially in functions related to feeding in insects,it is a molecular target for pest ***,an sNPF and 2 sNPFRs were identified and cloned from *** analysis revealed that the sNPF and its receptors had a highly conserved RLRFamide C-terminus and 7 transmembrane domains,*** sNPF and its receptor genes were distributed across larval periods and tissues,but 2 receptors had distinct expression *** starvation-induced assay elucidated that sNPF and sNPFR expression levels were downregulated under food deprivation and recovered with subsequent *** interference knockdown of sNPF,sNPFR1,and sNPFR2 by injection of double-stranded RNA into larvae not only suppressed food consumption and increased body size and weight,but also led to decrease of glycogen and total lipid contents,and increase of trehalose compared with double-stranded green fluorescent protein ***,molecular docking was performed on the interaction mode between sNPFR protein and its ligand sNPF based on the 3-dimensional models constructed by AlphaFold;the results indicated that both receptors were presumably activated by the mature peptide *** results revealed that sNPF signaling played a considerably vital role in the feeding regulation of *** and represents a potential control target for this pest.

关键词： feeding regulation Mythimna separata pest control target RNA interference short neuropeptide F sNPF receptors

基于NF-κB通路探究重楼皂苷Ⅶ的抗炎作用机制

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湖北医药学院学报 2024年第1期43卷 7-12,17页

作者：晋兰吴琼梁金美琪林迪斯郗雪艳郭阳湖北医药学院基础医学院湖北十堰442000 湖北医药学院附属国药东风总医院病理科湖北十堰442000

目的:探讨重楼皂苷Ⅶ(PSⅦ)的抗炎作用及机制。方法:利用MACS法从C57BL/6小鼠脾脏中分离初始T淋巴细胞。PSⅦ处理后,通过流式细胞术、RT-qPCR、Western blot检测PSⅦ对IL-6表达水平的影响;利用流式细胞术、Cell TraceTM Violet试剂盒检... 详细信息

目的:探讨重楼皂苷Ⅶ(PSⅦ)的抗炎作用及机制。方法:利用MACS法从C57BL/6小鼠脾脏中分离初始T淋巴细胞。PSⅦ处理后,通过流式细胞术、RT-qPCR、Western blot检测PSⅦ对IL-6表达水平的影响;利用流式细胞术、Cell TraceTM Violet试剂盒检测PSⅦ对CD4+T细胞活化及增殖分化的影响;利用Western blot检测PSⅦ对IκBα、STAT3及其磷酸化蛋白表达的影响;RNA-seq检测PSⅦ处理后的差异基因。结果:PSⅦ显著抑制IL-6的表达。PSⅦ对CD4+T细胞的活化及增殖无明显影响,但对其分化有一定影响。PSⅦ抑制p-IκBα、p-STAT3的蛋白表达水平,但对IκBα、STAT3无影响。RNA-seq分析的差异基因有70个(27个上调、43个下调),从中筛选出6个并利用RT-qPCR得到验证。结论:PSⅦ可通过STAT3和NF-κB信号通路抑制IL-6的表达,从而缓解炎症。

关键词：重楼皂苷Ⅶ NF-κB信号通路白细胞介素-6 炎性反应

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豆类蛋白聚集机制及调控措施研究进展

中国调味品 2024年第1期49卷 209-213页

作者：李美麒张学敏王金辰刁静静王长远徐旖欣曹荣安黑龙江八一农垦大学食品学院北大荒农产品加工现代产业学院黑龙江大庆163319 黑龙江八一农垦大学国家杂粮工程技术中心黑龙江大庆163319

目前在食品加工、化学工业生产以及生物医药等领域普遍存在蛋白质聚集的现象。近年来,豆类蛋白生产规模不断扩大,其营养价值及经济效价优势显著。基于此,越来越多的学者对豆类蛋白聚集、解聚行为进行了深入探讨。但豆类蛋白在食品加工... 详细信息

目前在食品加工、化学工业生产以及生物医药等领域普遍存在蛋白质聚集的现象。近年来,豆类蛋白生产规模不断扩大,其营养价值及经济效价优势显著。基于此,越来越多的学者对豆类蛋白聚集、解聚行为进行了深入探讨。但豆类蛋白在食品加工过程中会产生一些聚集行为,这是不可避免的现象。随着加工食品的手段越来越多样化,豆类蛋白的聚集行为对食品品质、口感、风味的影响也逐渐引起人们的重视。文章介绍了豆类蛋白聚集行为的产生机理,系统详尽地综述了调控豆类蛋白聚集行为的措施及对豆类蛋白聚集行为进行表征的方法。相信豆类蛋白在未来可以成为更好的蛋白质补充方式,有更加广泛的发展和应用空间,并希望为研究豆类蛋白聚集行为提供更多的理论支持。

关键词：豆类蛋白聚集行为调控措施研究进展

Rapid Hydrothermal Synthesis of Submillimeter Ultralong Flexible Hydroxyapatite Fiber Using Different pH Regulators

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Acta Metallurgica Sinica(English Letters) 2016年第7期29卷 609-613页

作者： mei-li qi Gui-yong Xiao Yu-peng Lu Key Laboratory for liquid-Solid Structural Evolution and Processing of Materials Ministry of EducationShandong University School of Materials Science and Engineering Shandong University

Hydroxyapatite fiber （HAF） was fabricated via a one-step hydrothermal route by using urea （CA）, acetamide （AA） and propanamide （PA） as pH regulators, respectively, and the reaction time was only 0.5 h. The HAF synthesized by CA as the pH regulator was agglomerated with irregular morphology, while using AA or PA as the pH regulator, the HAF was composed of well-dispersed fibers with lengths larger than 200 pm. Two different morphologies form mainly due to the different hydrolysis rates of the pH regulators. Most of the fibers synthesized by the three pH regulators showed curving appearance, implying their outstanding flexibility. The technique in this paper allows the rapid formation of flexible HAF and may have wider applications in the restorations and reinforcement composites.

关键词： Hydroxyapatite Fiber Hydrothermal PH regulators

Transcriptome Analysis of Heterosis in Survival in the Hybrid Progenies of‘Haida No.1’and Orange-Shelled lines of the Pacific Oyster Crassostrea gigas

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Journal of Ocean University of China 2024年第1期23卷 199-208页

作者： YANG Hang li qi Key Laboratory of Mariculture Ministry of EducationOcean University of ChinaQingdao 266003China

Heterosis has been exploited to enhance the yield and adaptability in various shellfish species;however,the molecular basis of it remains *** Pacific oyster Crassostrea gigas is one of the most economically important aquaculture species,and its productive traits can be improved by ***,an intraspecific cross between orange shell(O,10th generation)and‘Haida No.1’(H,13th generation)of *** was performed to assess the heterosis of survival *** rates of hybrid family(OH)and inbred families(HH and OO)were compared at larval stage,and eyed-pediveliger larvae of three families were subjected to transcriptome *** analysis results of best-parent heterosis and mid-parent heterosis showed that the hybrid family exhi-bited a high heterosis in survival relative to the parental *** OH-M(OH ***)and OH-P(OH ***)had 425 and 512 dif-ferentially expressed genes(DEGs),*** enrichment analysis of these DEGs revealed that the significantly enrich-ed genes function in virion binding,C-type lectin receptor signaling pathway,cellular defense response and other immune-related pro-cesses,which involves perlucin-like protein,CD209 antigen-like protein,ZNFX1,caspase-3 and acan *** differentially ex-pressed genes in OH-M and OH-P,together with the immune-related processes mentioned above may play an important role in the larval survival of *** addition,three genes(CYP450,fucolectin and perlucin-like)are associated with the orange shell and low survival of maternal oyster *** findings provide support for the application of hybrid with superior survival and will facilitate the understanding of heterosis formation in the Pacific oyster.

关键词： Crassostrea gigas survival rate heterosis transcriptome

Development of component stiffness equations for thread-fixed one-side bolt connections to an enclosed rectangular hollow section column under tension

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Frontiers of Structural and Civil Engineering 2024年第4期18卷 568-586页

作者： Fu-Wei WU Yuan-qi li Department of Structural Engineering Tongji UniversityShanghai 200092China State Key Laboratory of Disaster Reduction in Civil Engineering Tongji UniversityShanghai 200092China

The derivation and validation of analytical equations for predicting the tensile initial stiffness of threadfixed one-side bolts(TOBs),connected to enclosed rectangular hollow section(RHS)columns,is presented in this *** unknown stiffness components are considered:the TOBs connection and the enclosed RHS ***,the trapezoidal thread of TOB,as an equivalent cantilevered beam subjected to uniformly distributed loads,is analyzed to determine the associated *** on the findings,the thread-shank serial-parallel stiffness model of TOB connection is *** analysis of the tensile stiffness of the enclosed RHS face due to two bolt forces,the four sidewalls are treated as rotation constraints,thus reducing the problem to a two-dimensional plate *** to the load superposition method,the deflection of the face plate is resolved into three components under various boundary and load *** to the plate deflection theory of Timoshenko,the analytical solutions for the three deflections are derived in terms of the variables of bolt spacing,RHS thickness,height to width ratio,***,the validity of the above stiffness equations is verified by a series of finite element(FE)models of T-stub *** proposed component stiffness equations are an effective supplement to the component-based method.

关键词： initial stiffness component based method thread-fixed one-side bolt rectangular hollow sections analytical equation

Phase-field lattice-Boltzmann study on fully coupled thermal-solute-convection dendrite growth of Al-Cu alloy

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China Foundry 2024年第2期21卷 125-136页

作者： Yin-qi qiu Meng-wu Wu Xun-peng qin Shou-mei Xiong Hubei Key Laboratory of Advanced Technology for Automotive Components Wuhan University of TechnologyWuhan 430070China Hubei Longzhong Laboratory Xiangyang 441000HubeiChina School of Materials Science and Engineering Tsinghua UniversityBeijing 100084China

Dendrite growth is a complex liquid-solid phase transition process involving multiple physical factors.A phase-field lattice-Boltzmann method was developed to simulate the two-and three-dimension dendrite growth of Al-Cu *** effect of fully coupled thermal-solute-convection interaction on the dendrite growth was investigated by incorporating a parallel-adaptive mesh refinement algorithm into the numerical *** accurately reproducing the latent heat release,solute diffusion and convective transport behaviors at the liquidsolid interface,the interaction mechanism among thermal-solute-convection transport as well as their coupling effects on the dendrite growth dynamics were *** simulation results show that the release of latent heat slows down the dendrite growth rate,and both natural and forced convection disrupt the symmetrical growth of *** combination makes the growth of dendrites more complex,capturing important physical aspects such as recalescence,dendrite tip splitting,dendrite tilting,dendrite remelting,and solute plume in the simulation *** on the robustness and powerful ability of the numerical model,the formation mechanisms of these physical aspects were revealed.

关键词： simulation phase field dendrite growth thermal-solute-convection interaction

Comprehensive Evaluation Method for Traffic Flow Data Quality Based on Grey Correlation Analysis and Particle Swarm Optimization

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Journal of Systems Science and Systems Engineering 2024年第1期33卷 106-128页

作者： Wei Ba Baojun Chen qi li School of Automation and Electrical Engineering Dalian Jiaotong UniversityDalian116028China Faculty of Electronic Information and Electrical Engineering Dalian University of TechnologyDalian116023China

Nowadays,data are more and more used for intelligent modeling and prediction,and the comprehensive evaluation of data quality is getting more and more attention as a necessary means to measure whether the data are usable or ***,the comprehensive evaluation method of data quality mostly contains the subjective factors of the evaluator,so how to comprehensively and objectively evaluate the data has become a bottleneck that needs to be solved in the research of comprehensive evaluation *** order to evaluate the data more comprehensively,objectively and differentially,a novel comprehensive evaluation method based on particle swarm optimization(PSO)and grey correlation analysis(GCA)is presented in this *** first,an improved GCA evaluation model based on the technique for order preference by similarity to an ideal solution(TOPSIS)is ***,an objective function model of maximum difference of the comprehensive evaluation values is built,and the PSO algorithm is used to optimize the weights of the improved GCA evaluation model based on the objective function ***,the performance of the proposed method is investigated through parameter analysis.A performance comparison of traffic flow data is carried out,and the simulation results show that the maximum average difference between the evaluation results and its mean value(MDR)of the proposed comprehensive evaluation method is 33.24%higher than that of TOPSIS-GCA,and 6.86%higher than that of *** proposed method has better differentiation than other methods,which means that it objectively and comprehensively evaluates the data from both the relevance and differentiation of the data,and the results more effectively reflect the differences in data quality,which will provide more effective data support for intelligent modeling,prediction and other applications.

关键词： Data quality comprehensive evaluation particle swarm optimization grey correlation analysis traffic flow data