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Quantum Approach to Fast Protein-Folding Time

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Chinese Physics Letters 2019年第8期36卷 23-35页

作者： li-hua lu You-Quan li Zhejiang Province Key Laboratory of Quantum Technology&Device and Department of PhysicsZhejiang UniversityHangzhou 310027 Collaborative Innovation Center of Advanced Microstructure Nanjing UniversityNanjing 210008

In the traditional random-conformational-search model,various hypotheses with a series of meta-stable intermediate states were proposed to resolve the Levinthal paradox in protein-folding *** we introduce a quantum strategy to formulate protein folding as a quantum walk on a definite graph, which provides us a g’eneral framework without making *** it by the mean of first passage time,we find that the folding time via our quantum approach is much shorter than the one obtained *** random *** idea is expected to evoke more insights for future studies.

关键词： Quantum Fast Protein-Folding Time

Preparation of thermal barrier coatings by ultrasonic plasma spraying

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中国有色金属学会会刊：英文版 2004年第Z1期14卷 92-94页

作者： WEN Xiong-wei li lu-ming ZHANG hua-tang HAO Hong-wei lu Zhi-qing Department of Mechanical Engineering Tsinghua University Beijing 100084 China Department of Mechanical Engineering Tsinghua University Beijing 100084 China Department of Mechanical Engineering Tsinghua University Beijing 100084 China Department of Mechanical Engineering Tsinghua University Beijing 100084 China Department of Mechanical Engineering Tsinghua University Beijing 100084 China

Modulated plasma arc not only can heat the powder, but also can excite ultrasonic of different frequencies and different powers. The principles and characters of the plasma arc-excited ultrasonic were described, and the ultrasonic plasma spraying was compared with normal plasma spraying. Zirconia thermal barrier coatings (TBCs) were fabricated with two kinds of method. The TBCs were studied by the optical microscope observation, SEM observation and bonding strength experiment. The results show that suitable ultrasonic changes the performance and microstructure of TBCs in evidence. And the mechanism of ultrasonic influencing the TBCs was also discussed.

关键词： plasma spraying ultrasonic thermal barrier coatings

The water-saving potential of using micro-sprinkling irrigation for winter wheat production on the North China Plain

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Journal of Integrative Agriculture 2021年第6期20卷 1687-1700页

作者： ZHAI li-chao lu li-hua DONG Zhi-qiang ZHANG li-hua ZHANG Jing-ting JIA Xiu-ling ZHANG Zheng-bin Institute of Cereal and Oil Crops Hebei Academy of Agriculture and Forestry Sciences/Scientific Observing and Experimental Station of Crop Cultivation in the North ChinaMinistry of Agriculture and Rural AffairsShijiazhuang 050035P.R.China Center for Agricultural Resources Research Institute of Genetics and Development BiologyChinese Academy of SciencesShijiazhuang 050021P.R.China

The shortage of groundwater resources is a considerable challenge for winter wheat production on the North China ***-saving technologies and procedures are thus urgently *** determine the water-saving potential of using micro-sprinkling irrigation(MSI)for winter wheat production,field experiments were conducted from 2012 to *** to traditional flooding irrigation(TFI),micro-sprinkling thrice with 90 mm water(MSI1)and micro-sprinkling four times with 120 mm water(MSI2)increased the water use efficiency by 22.5 and 16.2%,respectively,while reducing evapotranspiration by 17.6 and 10.8%.Regardless of the rainfall pattern,MSI(i.e.,MSI1 or MSI2)either stabilized or significantly increased the grain yield,while reducing irrigation water volumes by 20–40%,compared to *** the same volumes of irrigation water,MSI(i.e.,MSI3,micro-sprinkling five times with 150 mm water)increased the grain yield and water use efficiency of winter wheat by 4.6 and 11.7%,respectively,compared to *** MSI could supply irrigation water more frequently in smaller amounts each time,it reduced soil layer compaction,and may have also resulted in a soil water deficit that promoted the spread of roots into the deep soil layer,which is beneficial to photosynthetic production in the critical *** conclusion,MSI1 or MSI2 either stabilized or significantly increased grain yield while reducing irrigation water volumes by 20–40%compared to TFI,and should provide water-saving technological support in winter wheat production for smallholders on the North China Plain.

关键词： winter wheat grain yield water use efficiency micro-sprinkling irrigation traditional flooding irrigation water-saving potential

Data Mining Ontology Development for High User Usability

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Wuhan University Journal of Natural Sciences 2006年第1期11卷 51-56页

作者： li Yu-hua lu Zheng-ding SUN Xiao-lin WEN Kun-mei li Rui-xuan College of Computer Science and Technology Huazhong University of Science and Technology Wuhan 430074Hubei China

This paper mainly introduces the development and implementation of the user centered data mining service ontology on Universal Knowledge Grid （UKG）. UKG is an ontology-based grid architecture model to build large-scale distributed knowledge discovery system on the grid. The data mining ontology services are the main service offering by UKG. It can meet the user requirements of knowledge discovery in different domains and different hierarchies and make the system exoteric, extensible and high usable. A data min- ing solution for money laundering is introduced.

关键词： data mining ontology usability universal knowledge grid

Dynamic Accuracy Design Method of Ultra-precision Machine Tool

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Chinese Journal of Mechanical Engineering 2018年第1期31卷 167-175页

作者： Guo-Da Chen Ya-Zhou Sun Fei-Hu Zhang li-hua lu Wan-Qun Chen Nan Yu Center for Precision Engineering Harbin Institute of Technology Key Laboratory of E&M Ministry of Education & Zhejiang Province Zhejiang University of Technology Precision Engineering Institute Cranfield University

Ultra-precision machine tool is the most important physical tool to machining the workpiece with the frequency domain error requirement, in the design process of which the dynamic accuracy design(DAD) is indispensable and the related research is rarely available. In light of above reasons, a DAD method of ultra-precision machine tool is proposed in this paper, which is based on the frequency domain error *** basic procedure and enabling knowledge of the DAD method is introduced. The application case of DAD method in the ultra-precision flycutting machine tool for KDP crystal machining is described to show the procedure detailedly. In this case, the KDP workpiece surface has the requirements in four different spatial frequency bands, and the emphasis for this study is put on the middle-frequency band with the PSD specifications. The results of the application case basically show the feasibility of the proposed DAD method. The DAD method of ultra-precision machine tool can effectively minimize the technical risk and improve the machining reliability of the designed machine tool. This paper will play an important role in the design and manufacture of new ultra-precision machine tool.

关键词： Dynamic accuracy design Ultra-precision machine tool Frequency domain Error allocation

An Enzymatic Reaction Modulated Fluorescence-on Omethoate Biosensor Based on Fe_(3)O_(4)@GO and Copper Nanoparticles

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Journal of Analysis and Testing 2022年第1期6卷 58-66页

作者： Hui Zhang Wu-Kui Kang Ying Wang Xiu-Zhong Wang li-hua lu College of Chemistry and Pharmaceutical Sciences Qingdao Agricultural UniversityQingdao 266109China

The employing of organophosphorus pesticides(OPs),especially,the abusing of OPs,leads to residue accumulation,which causes immense effect to human health and ***,it is an urgent task to develop highly sensitive OPs’detection platforms.A novel enzyme modulated fluorescence-on sensor for sensitive detection of omethoate was successfully constructed,using AT-rich double-stranded DNA(dsDNA)templated copper nanoparticles(CuNPs)as the fluorescent signal and covalently combined magnetic Fe_(3)O_(4)and graphene oxide(GO)as the single-stranded DNA(ssDNA)*** assay just associates acetylcholinesterase(AChE),one strand of dsDNA,acetylcholinein(ATCh),Fe_(3)O_(4)@GO and ascorbic acid(AA)/Cu^(2+).T6-1 and T6-2 are two strands single-stranded DNA(ssDNA)with the AT-rich sequence and continuous T bases at their two ends,*** the aid of Hg^(^(2+)),they can hybridize into a blunt-ended dsDNA with one AT-rich end and one T-Hg^(^(2+))-T base pairs *** omethoate exists,it can inhibit AChE from transducing acetylcholinein(ATCh)into thiocholine(TCh).So,no TCh can snatch Hg^(^(2+))from T-Hg^(^(2+))-T base pairs in blunt-ended T6-1/T6-2.T6-1/T6-2 still keeps its integrity in blunt-ended dsDNA configuration,and hence the subsequently added Fe_(3)O_(4)@GO cannot absorb *** remained blunt-ended T6-1/T6-2 in supernatant can act as the template of CuNPs to produce strong *** developed detection offers a signal-on omethoate detection,which can sensitively detect omethoate in the linear range of 5-200 nmol/L with a detection limit of 2.48 nmol/*** importantly,it can detect omethoate in food and environmental samples,demonstrating high potential in real sample detection.

关键词： Fe_(3)O_(4)@GO AT-rich DNA Copper nanoparticles Omethoate Fluorescence

Investigation on Azoospermia Factor (AZF) Microdeletion and Sex-determining Region Y (SRY) of the Y Chromosome in Male Infertility

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Journal of Reproduction and Contraception 2013年第2期24卷 88-94页

作者： li-hua lu Fei-xue HU Song GE Laboratory of Genetics Deptment of Laboratory Medicine Peking University Shenzhen Hospital

Objective To determine the incidence of azoospermia faetor （AZF） microdeletions of Y chromosome in male infertility and to investigate the mechanism of sex-determining region Y （SRY） in sex differentiation. Methods The mierodeletion of AZF was detected by multiplex polymerase chain reaction （PCR） using Y-chromosome specific sequence tagged sites （STSs）, and SRY was analyzed by PCR and sequencing. Results There were 100 cases with AZF microdeletion and the ratio of AZF microdeletion was 6.8% over all 1 474 cases. The ratios of AZF microdeletion of azoospermia group and severe oligozoospermia group were 9.0% and 7.1%, respectively, which was significantly different from oligozoospermia group （P〈 0. 05）. There were 67 cases with 5 STSs mierodeletion of sY152, sY239, sY243, sY254 and sY255. There were 20 cases with long fragment deletion more than 10 STSs, and the patterns of AZF microdeletion in other 13 cases were rare. In all 9 patients with disorders of sex differentiation, there were 6patients with SRY-absent and AZF-absent. There was no mutation of SRY gene by sequencing in other 3 patients with SRY-positive. Conclusion Deletions in AZF region of Y chromosome are specific with diagnoses with spermatogenesis disorder. Deletions of sY152, sY239, sY243, sY254 and sY255 occur the most frequently. SRY was an important candidate gene of testis-determining factor （TDF） gene.

关键词： sex-determining region Y (SRY) azoospermia factor (AZF) sex differentiation spermatogenesis

Delivering DNA into Plant Cell by Gene Carriers of ZnS Nanoparticles

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Chemical Research in Chinese Universities 2012年第4期28卷 672-676页

作者： FU Yu-qin li lu-hua WANG Pi-wu QU Jing FU Yong-ping WANG Hui SUN Jing-ran LÜ Chang-li College of life Science Jilin Agricultural UniversityChangchun 130018P.R.China Faculty of Agronomy Jilin Agricultural UniversityChangchun 130018P.R.China Faculty of Chemistry Northeast Normal UniversityChangchun 130024P.R.China

The development of nanotechnology provides a new method for genetic ***,the nanoparticles as gene carriers have been mainly used in the mammalian cells so *** observed that ZnS nanoparticles modified with positively charged poly-L-lysine（PLL） successfully delivered GUS-encoding plasmid DNA into tobacco cells by means of ultrasound-assisted *** chain reaction（PCR） detection,Southern blot analysis and GUS histochemical staining were carried out for the regenerated *** stable genetic modified plants mediated by ZnS nanoparticles can be *** article demonstrates the great potential of nanoparticles as gene carrier in plant transformation and proves a novel approach for plant genetic decoration.

关键词： ZnS nanoparticle Gene carrier Transformation Tobacco Stable expression

Global Positioning Scheme via Quantum Teleportation

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Chinese Physics Letters 2023年第11期40卷 18-24页

作者：李有泉吕丽花朱启航 Chern Institute of Mathematics Nankai UniversityTianjin 300071China School of Physics Zhejiang UniversityHangzhou 310058China Collaborative Innovation Center of Advanced Microstructure Nanjing UniversityNanjing 210093China

Quantum teleportation scheme is undoubtedly an inspiring theoretical discovery as an amazing application of quantum physics,which was experimentally realized several years *** the purpose of quantum communication via this scheme,an entangled ancillary pair shared by Alice and Bob is the essential ingredient,and a quantum memory in Bob’s system is necessary for him to keep the quantum state until the classical message from Alice ***,the quantum memory remains a challenge in both technology and *** we show that quantum teleportation provides fresh perspectives in terms of an alternative scheme for global positioning *** to fixed locations of Bob and Charlie,Alice can determine her relative position by comparing quantum states before and after teleporting around via Bob and Charlie *** may open up a new scene in the stage of the application of quantum physics without quantum memories.

关键词： quantum scheme rational