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Delivering DNA into Plant Cell by Gene Carriers of ZnS Nanoparticles

Delivering DNA into Plant Cell by Gene Carriers of ZnS Nanoparticles

作     者:FU Yu-qin LI Lu-hua WANG Pi-wu QU Jing FU Yong-ping WANG Hui SUN Jing-ran LÜ Chang-li 

作者机构:College of Life ScienceJilin Agricultural UniversityChangchun 130018P.R.China Faculty of AgronomyJilin Agricultural UniversityChangchun 130018P.R.China Faculty of ChemistryNortheast Normal UniversityChangchun 130024P.R.China 

出 版 物:《Chemical Research in Chinese Universities》 (高等学校化学研究(英文版))

年 卷 期:2012年第28卷第4期

页      面:672-676页

核心收录:

学科分类:080903[工学-微电子学与固体电子学] 07[理学] 0809[工学-电子科学与技术(可授工学、理学学位)] 08[工学] 09[农学] 071007[理学-遗传学] 090102[农学-作物遗传育种] 0710[理学-生物学] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程(可授工学、理学学位)] 080502[工学-材料学] 0901[农学-作物学] 0836[工学-生物工程] 

基  金:Supported by the National Natural Science Foundation of China(No.21074019) the China Postdoctoral Science Foundation Funded Project(No.200904501024) the Natural Science Foundation of Jilin Province,China(No.20101539) the Jilin Province Science and Technology Development Project,China(No.20090155) 

主  题:ZnS nanoparticle Gene carrier Transformation Tobacco Stable expression 

摘      要:The development of nanotechnology provides a new method for genetic ***,the nanoparticles as gene carriers have been mainly used in the mammalian cells so *** observed that ZnS nanoparticles modified with positively charged poly-L-lysine(PLL) successfully delivered GUS-encoding plasmid DNA into tobacco cells by means of ultrasound-assisted *** chain reaction(PCR) detection,Southern blot analysis and GUS histochemical staining were carried out for the regenerated *** stable genetic modified plants mediated by ZnS nanoparticles can be *** article demonstrates the great potential of nanoparticles as gene carrier in plant transformation and proves a novel approach for plant genetic decoration.

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