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微藻生物肥提高谷子对氮磷胁迫的耐受性

植物营养与肥料学报 2024年第6期30卷 1130-1141页

作者：李慧敏李炯珊张克燕张春辉季春丽王计平李润植山西农业大学农学院/分子农业与生物能源研究所/山西省特用作物遗传和代谢工程研究中心山西晋中030801

【目的】氮磷营养缺乏是作物生长发育的重要限制因素。微藻生物肥是由单细胞光合藻类生物质构成的一种新型肥料,其促进作物生长和改良土壤肥力的功效备受关注。本研究旨在解析微藻生物肥对谷子(Setaria italica L.)生长的促进作用,特别... 详细信息

【目的】氮磷营养缺乏是作物生长发育的重要限制因素。微藻生物肥是由单细胞光合藻类生物质构成的一种新型肥料,其促进作物生长和改良土壤肥力的功效备受关注。本研究旨在解析微藻生物肥对谷子(Setaria italica L.)生长的促进作用,特别是提高谷子对氮磷缺乏的耐受性的生物学功能,为微藻生物肥在谷子等作物的化肥减量和绿色可持续生产的应用提供理论支撑。【方法】供试谷子品种为‘晋谷21’。采用水培试验,设置正常氮磷(霍格兰标准营养液中氮和磷含量分别为210和71 mg/L)、无氮磷(营养液中氮和磷含量均为0 mg/L)和1/2氮磷(营养液中氮和磷含量分别为105和35.5 mg/L)3个氮磷水平。制备小球藻(Chlorella ***01)(DT)、埃氏小球藻(Chlorella emersonii)(AS)和蛋白核小球藻(Chlorella pyrenoidosa)(DB)3种微藻生物肥,分别与3个氮磷处理液等比混合,共构成9个处理,每个氮磷水平下设置1个不添加微藻微生物溶液处理为对照。在谷子幼苗生长至两叶一心时,将其移入处理液中,在温室条件下连续培养9天,期间每3天补充1次处理液。处理结束时,测定各处理谷子幼苗的生长指标、生物量、色素含量、光合活性和氮磷含量等生理生化参数,并通过qRT-PCR技术分析氮磷转运蛋白相关基因(SiNRT1.11、SiNRT2.1、SiPHT1.2和SiPHT1.1)的表达谱。【结果】与正常氮磷处理相比,无氮磷和1/2氮磷处理均显著降低了谷子幼苗生物量、光合色素(叶绿素a,b和类葫萝卜素)含量和光合作用。在3种剂量氮磷处理下,施用3种微藻生物肥处理均显著提高谷子幼苗鲜重和干重、光合色素含量(特别是类胡萝卜素)和光合作用参数[实际光化学效率Y(II),光化学淬灭系数qP和非光化学淬灭系数NPQ]。此外,微藻生物肥还显著增加了谷子幼苗全氮和全磷含量。微藻生物肥DT处理的促生效应最显著。qRT-PCR检测谷子幼苗氮磷转运相关基因表达显示,微藻生物肥DT处理显著上调谷子幼苗地上部硝酸盐转运蛋白基因SiNRT1.11和根系硝酸盐转运蛋白基因SiNRT2.1的表达,以及根系中磷酸盐转运蛋白基因SiPHT1.2和SiPHT1.1的表达。【结论】施用微藻生物肥能显著提高谷子幼苗地上部硝酸盐转运蛋白基因SiNRT1.11和根系硝酸盐转运蛋白基因SiNRT2.1,以及根系中磷酸盐转运蛋白基因SiPHT1.2和SiPHT1.1的表达,进而改善谷子幼苗生长和光合生理特性,提高谷子对氮磷胁迫的抗性。

关键词：谷子(Setaria italica L.) 微藻生物肥氮磷缺乏生理生化特性基因表达

大学生隐性自恋人格与网络失范行为的关系:自我意识与人际敏感的链式中介作用

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黑龙江高教研究 2024年第1期42卷 140-146页

作者：林启修马向真李慧敏南京工业大学江苏南京211816 东南大学江苏南京211189 南京审计大学江苏南京211815

随着网络时代的到来,大学生网络失范行为已成为社会关注的焦点。采用隐性自恋问卷、自我意识量表、人际关系敏感量表、网络道德行为问卷对822名大学生进行调查,探讨大学生隐性自恋人格对其网络失范行为的影响以及自我意识和人际敏感在... 详细信息

随着网络时代的到来,大学生网络失范行为已成为社会关注的焦点。采用隐性自恋问卷、自我意识量表、人际关系敏感量表、网络道德行为问卷对822名大学生进行调查,探讨大学生隐性自恋人格对其网络失范行为的影响以及自我意识和人际敏感在其中的链式中介作用。结果表明:一是隐性自恋人格、自我意识、人际敏感及网络失范行为两两之间均显著相关;二是隐性自恋人格对大学生网络失范行为的直接效应显著,也分别由自我意识、人际敏感的中介及二者的链式中介产生间接效应。具有隐性自恋人格的大学生可通过提高自我意识和降低人际敏感性来减少网络失范行为。

关键词：隐性自恋人格网络失范行为自我意识人际敏感

玉米茎腐病致病菌全基因组分泌蛋白预测与分析

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玉米科学 2024年第4期32卷 110-118页

作者：曹言勇张立立李慧敏韩胜博侯梦薇段灿星施艳张新友河南省农业科学院粮食作物研究所/神农种业实验室郑州450002 中国农业科学院作物科学研究所北京100081 河南农业大学植物保护学院郑州450002

九州镰孢菌(Fusarium kyushuense)是一种重要的植物病原菌,可引起玉米茎腐病。为明确九州镰孢菌的分泌蛋白,运用SignalP 6.0、ProtComp 9.0、TMHMM 2.0、big-PI Predictor、TargetP 2.0等软件对九州镰孢菌全基因组12225条蛋白质序列进... 详细信息

九州镰孢菌(Fusarium kyushuense)是一种重要的植物病原菌,可引起玉米茎腐病。为明确九州镰孢菌的分泌蛋白,运用SignalP 6.0、ProtComp 9.0、TMHMM 2.0、big-PI Predictor、TargetP 2.0等软件对九州镰孢菌全基因组12225条蛋白质序列进行分泌蛋白的预测和筛选。结果表明,九州镰孢菌195全基因组蛋白中共有539条序列具有典型的分泌蛋白特征,占总序列数的4.41%。信号肽-3和-1位置的氨基酸相对保守,其切割位点属于A-X-A型。分泌蛋白功能注释分析结果表明,九州镰孢菌的分泌蛋白中共有264个效应蛋白,其功能主要涉及细胞壁组分降解。对碳水化合物活性酶类(CAZymes)进行预测发现,九州镰孢菌分泌蛋白组中共有168个碳水化合物活性酶,为进一步探究其生物学特性、致病机制和筛选新的镰孢菌致病效应蛋白奠定基础。

关键词：玉米九州镰孢菌生物信息学分泌蛋白

Understanding systemic risk induced by climate change

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Advances in Climate Change Research 2021年第3期12卷 384-394页

作者： li hui-min WANG Xue-Chun ZHAO Xiao-Fan QI Ye Beijing Climate Change Response Research and Education Center Beijing University of Civil Engineering and ArchitectureBeijing100044China School of Public Policy and Management Tsinghua UniversityBeijing100084China Division of Public Policy The Hong Kong University of Science and TechnologyHong KongChina Institute for Public Policy The Hong Kong University of Science and Technology(Guangzhou)GuangzhouChina

The systemic risk induced by climate change represents one of the most prominent threats facing humanity and has attracted increasing attention since the outbreak of the COVID-19 pandemic at the end of *** existing literature highlights the importance of systemic risk induced by climate change,but there are still deficiencies in understanding its dynamics and assessing the *** to bridge this gap,this study develops a theoretical framework and employs two cases to illustrate the concept,origin,occurrence,propagation,evolution,and assessment framework of systemic risk induced by climate *** key findings include:1)systemic risk induced by climate change derives from the rapid growth of greenhouse gas,emissions,increasingly complex connections among different socioeconomic systems,and continuous changes in exposure and vulnerability;2)systemic risk induced by climate change is a holistic risk generated by the interconnection,interaction,and dynamic evolution of different types of single risks,and its fundamental,defining feature is cascading *** extent of risk propagation and its duration depend on the characteristics of the various discrete risks that are connected to make up the systemic risk;3)impact domains,severity of impact,and probability of occurrences are three core indicators in systemic risk assessment,and the impact domains should include the economy,society,homeland security,human health,and living *** propose to deepen systemic risk research from three aspects:to develop theories to understand the mechanism of systemic risk;to conduct empirical research to assess future risks;and to develop countermeasures to mitigate the risk.

关键词： Systemic risk Climate change Disaster Risk assessment

Heat stability of winter wheat depends on cultivars, timing and protective methods

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Journal of Integrative Agriculture 2020年第8期19卷 1984-1997页

作者： li Qiang CHANG Xu-hong MENG Xiang-hai li Ding ZHAO ming-hui SUN Shu-luan li hui-min QIAO Wen-chen Dryland Farming Institute Hebei Academy of Agriculture and Forestry SciencesHengshui 053000P.R.China Key Lab of Crop Drought Tolerance Research of Hebei Province Hengshui 053000P.R.China Institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijing 100081P.R.China

Heat stress negatively affects wheat production in many regions of the *** present,sensitivity to heat stress remains one of the least understood aspects of wheat genetics and breeding,and measures for preventing heat stress are *** this study,we used three cultivars of winter wheat(GY2018,SL02-1 and SY20)to evaluate the effect of heat stress at different days after anthesis(DAA)on yield and *** stability of the cultivars were analyzed and evaluated for the effects of two kinds of regulators on wheat under heat stress *** treatment at 7 DAA led to the most substantial reduction in yield while GY2018 had the best heat stability with respect to yield,and demonstrated the most positive effects on several quality traits including protein content,sedimentation volume and glutenin and gliadin *** treatment at 14 DAA had the least reduction in yield,while SY20 had the best heat stability with respect to yield and heat treatment had minimal effects on *** treatment at 21 DAA had only a limited effect on yield,while SL02-1 had the best heat stability with respect to yield,but it showed the most negative effects on *** time at 14 DAA and protein content at 21 DAA can be used as indicators for detecting the stability of quality under heat *** the three studied cultivars,SY20 was the most sensitive to heat stress with the stable time decreasing from 26.4 to 9.1 min,a higher sedimentation volume at 7 DAA,and a lowerγ-gliadin content which increased 2.4-fold under high-temperature *** addition of various regulators had different effects:potassium dihydrogen phosphate(KDP)was more protective of yield with heat stress at 7 DAA,while Duntianbao(DTB)had better effects on quality with heat stress at 21 DAA.

关键词： wheat high temperature heat stability yield quality prevention

IFN-γ release assay： a diagnostic assistance tool of tuberculin skin test in pediatric tuberculosis in China

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Chinese Medical Journal 2010年第20期123卷 2786-2791页

作者： SUN lin YAN hui-min HU Ying-hui JIAO Wei-wei GU Yi XIAO Jing li hui-min JIAO An-xia GUO Ya-jie SHEN A-dong Beijing Pediatric Institute Beijing Children's Hospital Affiliated to Capital Medical UniversityBeijing 100045China Department of Internal Medicine Beijing Children's Hospital Affiliated to Capital Medical UniversityBeijing 100045China Department of Respiratory Medicine Beijing Children's Hospital Affiliated to Capital Medical UniversityBeijing 100045China Department of Respiratory Medicine Beijing Children's Hospital Affiliated to Capital Medical UniversityBeijing 100045China) JIAO An-xia(Beijing Pediatric InstituteBeijing Children's Hospital Affiliated to Capital Medical UniversityBeijing 100045China

Background Prompt diagnosis of Mycobacterium tuberculosis （MTB） infection is an essential step in tuberculosis control and elimination. However, it is often difficult to accurately diagnose pediatric tuberculosis （TB）. The tuberculin test （TST） may have a low specificity because of cross-reactivity with antigens present in Mycobacterium bovis bacillus Calmette-Guerin （BCG） and other mycobacteria, especially in China with a predominantly BCG-vaccinated ***-secreted antigenic target 6-kDa protein （ESAT-6） and culture filtrate protein 10 （CFP-10）, stand out as suitable antigens that induce an interferon-gamma （IFN-γ） secreting, T-cell-mediated immune response to infection. While,considered the higher costs and complexity of the IFN-γ release assay （TSPOT）, we aimed to evaluate the TSPOT and TST test in the clinical diagnosis of pediatric tuberculosis and to establish a diagnostic process suitable for *** The sensitivity and specificity of the assay were evaluated in total seventy four children with active tuberculosis and fifty one nontuberculous children with other disease, and then the results were compared with *** regression models were used to identify variables that were associated with positive results for each assay. The independent variables included sex, age, birth place, vaccination history, close contract with an active TB *** The sensitivity of TSPOT was higher than TST in active TB children with or without BCG vaccination, as well as in children with culture-confirmed TB. But the difference was not significant statistically. Combining results of the TSPOT and TST improved the sensitivity to 94.6%. Agreement of the TST and TSPOT was low （77.0%, k=0.203） in active TB patients. The difference in specificity between TSPOT and TST test was statistically significant （94.1% vs.70.6%, P=0.006）. Specificity of the two tests in patients without prior BCG vaccination history was similar （80.0% vs.60.0%）. The concordance be

关键词： interferon-γ early-secreted antigenic target 6 diagnosis tuberculosis children

基于模糊层次分析法(Fuzzy-AHP)的船舶柴油机整机海上运输安全环境风险研究

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中国水运 2024年第8期 27-30页

作者：李惠敏刘解放胡雪春湖北交通职业技术学院湖北武汉430079

船舶柴油机整机海上运输系统在实际运作中较为复杂,此文以模糊层次分析法为基础,通过问卷调查方式收集业内专家的数据,对船舶柴油机整机海上运输安全环境风险进一步做出了较为细致的研究,识别出的环境影响因子权重可为船舶柴油机整机海... 详细信息

船舶柴油机整机海上运输系统在实际运作中较为复杂,此文以模糊层次分析法为基础,通过问卷调查方式收集业内专家的数据,对船舶柴油机整机海上运输安全环境风险进一步做出了较为细致的研究,识别出的环境影响因子权重可为船舶柴油机整机海上运输安全提供重要的理论参考。

关键词：模糊层次分析法整机运输风险研究

Evaluation of a new method for quantification of heat tolerance in different wheat cultivars

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Journal of Integrative Agriculture 2018年第4期17卷 786-795页

作者： li Qiang WANG Zheng-rui li Ding WEI Jian-wei QIAO Wen-chen MENG Xiang-hai SUN Shu-luan li hui-min ZHAO ming-hui CHEN Xiu-min ZHAO Feng-wu Dryland Farming Institute Hebei Academy of Agricultural and Forestry Sciences/Key Laboratory of Crop Drought Tolerance Research of Hebei Province

Heat stress seriously affects wheat production in many regions of the world. At present, heat tolerance research remains one of the least understood fields in wheat genetics and breeding and there is a lack of effective methods to quantify heat stress and heat tolerance in different wheat cultivars. The objective of this study was to use various wheat cultivars to evaluate stress intensity(δ) and a new method for quantification of heat tolerance and compare this technique with three other currently utilized methods. This new parameter for heat tolerance quantification is referred to as the heat tolerance index(HTI) and is an indicator of both yield potential and yield stability. Heat treatments were applied in a controlled setting when anthesis had been reached for 80% of the wheat. The stress intensity evaluation indicated heat shock was the main factor associated with kernel weight reduction while grain yield reduction was mainly associated with chronic high temperature. The methods evaluation showed that a temperature difference of 5°C from natural temperatures was a suitable heat treatment to compare to the untreated controls. HTI was positively correlated with yield under heat stress(r=0.8657, δ=0.15, in 2009–2010; r=0.8418, δ=0.20, in 2010–2011; Plidated the use of HTI and temperature difference control for quantifying wheat heat tolerance that included the yield potential and the stability of different wheat cultivars under heat stress. Additionally, 10 wheat cultivars showed high HTI and should be further tested for their heat confirming characteristics for use in wheat heat tolerance breeding.

关键词： wheat breeding heat tolerance quantification HTI temperature difference controlling stress intensity

基于主成分分析和聚类的营运车辆道路行驶工况构建研究

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汽车科技 2024年第4期 47-54页

作者：杜宇程李会民宋尚斌晋杰交通运输部公路科学研究院北京100088

《营运客车燃料消耗量限值及测量方法》和《营运货车燃料消耗量限值及测量方法》系列标准是加快我国营运车辆绿色低碳运输管理所依据的重要标准。修订两项标准一方面结合国内外汽车技术水平的迅速提升及道路运输的迅猛发展状况,另一方... 详细信息

《营运客车燃料消耗量限值及测量方法》和《营运货车燃料消耗量限值及测量方法》系列标准是加快我国营运车辆绿色低碳运输管理所依据的重要标准。修订两项标准一方面结合国内外汽车技术水平的迅速提升及道路运输的迅猛发展状况,另一方面对促进实现交通运输节能减排目标、缓解石油资源短缺、降低营运车辆的碳排放量等具有重大的现实意义。其中,适时调整试验各类工况时间占比系数是两标准修订的重要部分,需结合我国当前的地理类型、道路交通规则、道路结构等对实际行驶工况进一步研究分析。本文以营运货车为例,基于各大主机厂车联网平台系统的数据采集来源,利用数据清洗、短行程筛选、数据规约和聚类等方法分析采集数据,能够比较准确地反映营运车辆实际道路行驶特征。为营运车辆燃料消耗量各工况系数调整提供了技术方案和有力的数据支撑。

关键词：营运车辆行驶工况燃料消耗量降维聚类

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