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Spermatozoal protein profiles in male infertility with asthenozoospermia

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Chinese Medical Journal 2010年第20期123卷 2879-2882页

作者： li Hong-ju YU Ning ZhanG Xin-yu JIN Wei li han-zhong Department of Urology Peking Union Medical College Hospital Chinese Academy of Medical Sciences Beijing 100730 China

Background Infertility is a major medical and social problem, and elementary research on the spermatozoal proteins and their functions are relatively scarce and there are very few confirmed and effective options for the treatment of male infertility. Thus, it is essential to find candidate proteins that affect male infertility. This study was designed to detect the proteins with differential expression in sperm from infertile patients and normal *** Semen samples from patients with idiopathic asthenozoospermia （n=114） and from fertile men with normal spermiograms （n=37） were collected. Semen sample analysis, sperm protein extraction, SDS-PAGE electrophoresis and Western blotting analysis were performed. Results were analyzed by SPSS 16.0 statistical *** Western blotting analysis of spermatic proteins displayed a major differentially expressed protein in spermatozoa from fertile and idiopathic asthenozoospermia patients. Densities and volumes of the identified protein in the patients were significantly decreased compared to normal donors （P=0.034 and P=0.036, respectively）. The protein was identified as DEAD-box protein 4 （DDX4, VASA）. The expression and correction value （CV） of DDX4/VASA in the patients was reduced significantly compared to normal donors （P=0.037 and P=0.031, respectively）.Conclusions The expression of spermatic protein DDX4/VASA associates with spermatic motility, implying that DDX4NASA may be a candidate marker for evaluation of spermatic motility.

关键词： male infertility asthenozoospermia proteomics

Castleman＇s Disease： A Rare Mass in the Pararenal Retroperitoneum that Mimics Other Tumors

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Chinese Medical Journal 2017年第17期130卷 2126-2127页

作者： Yi Xie Yi Zhao Zhi-Gang Ji han-zhong li Guang-Hua liu Quan-zhong Mao Department of Urology Peking Union Medical College Hospital Chinese Academy of Medical Sciences Beijing 100730 China Department of Neurosurgery Peking Union Medical College Hospital Chinese Academy of Medical Sciences Beijing 100730 China

Castleman＇s disease （CD） is a rare lymphoproliferative syndrome typically involving a mediastinal mass. CD in the pararenal area should be distinguished from lymphoma diseases. We analyzed the clinical features often CD patients in the pararenal retroperitoneum that mimics other tumors to summarize some of our experiences and conduct a literature review.

关键词： Castleman's Disease Mimic Pararenal Retroperitoneum

Role of adrenalectomy in recurrent Cushing＇s disease

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Chinese Medical Journal 2010年第13期123卷 1658-1662页

作者： DING Xue-fei li han-zhong YAN Wei-gang GAO Ying li Xiao-qiang Department of Urology Peking Union Medical College Hospital Peking Union Medical College & Chinese Academy of Medical Sciences Beijing 100730 China Department of Obstetrics and Gynecology Department of Urology Affiliated Hospital of North China Coal Medical College Tangshan Hebei 063000 China

Background Cushing＇s disease is a pituitary-dependent type of Cushing＇s syndrome. Treatment consists of pituitary surgery or radiotherapy, but the recurrence rate at 10 years is as high as 40%. Adrenalectomy is considered an effective treatment to refractory Cushing＇s disease. The objective of this study was to examine the efficacy of laparoscopic adrenalectomy and open adrenalectomy in Cushing＇s disease, focusing on reversing the sequelae of hypercortisolism and improving patients＇ quality of life. Methods Forty-three patients （29 women, 14 men） with recurrent Cushing＇s disease after transsphenoidal operation underwent laparoscopic （n=32） or open （n=11） adrenalectomy from 2000 to 2008. Surgical results were evaluated for all the 43 patients. Patients completed a follow-up survey, including the short-form 36-item （SF-36） health survey. Results All the 43 patients achieved clinical reversal of hypercortisolism after adrenalectomy. Time to symptom resolution varied from a few weeks to up to 3 years. Most physical changes had resolved by a mean of 8 months after surgery. These conditions were not significantly different between the laparoscopy and open groups. Median length of hospital stay was shorter in the laparoscopy group （4 vs. 9 days; P 〈0.001）. Median follow-up was 48.5 months. Of the 34 （79%） patients available for follow-up, 22 （65%） had adrenocorticotropic hormone levels 〉200 ng/ml and 6 （27%） had clinical Nelson syndrome. Four patients died by 75 months after surgery. Using SF-36, 30 （88%） patients reported they felt their health status was good to excellent compared with 1 year before adrenalectomy; however, they showed significantly lower scores in all the 8 SF-36 parameters compared with the general population. No significant difference emerged in SF-36 scores between the laparoscopy and open groups. Conclusions Adrenalectomy showed high survival and clinical benefits in recurrent Cushing＇s disease patients. Despite patient-reported improvement in health

关键词： adrenal gland Cushing's disease adrenalectomy laparoscopy

Endovascular Treatment of the Huge Dissecting Aneurysms Involving the Basilar Artery by the Internal Trapping Technique： Technical Note

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Chinese Medical Journal 2015年第14期128卷 1916-1921页

作者： Shi-Qing Mu Xin-Jian Yang You-Xiang li Chu-han Jiang zhong-Xue Wu Department of Interventional Neuroradiology Beijing Neurosurgical Institute and Beijing Tiantan Hospital Capital Medical University Beijing 100050 China

Background： The endovascular strategy of the huge dissecting aneurysms involving the basilar artery （BA） is controversial and challenging. This study was to investigate the clinical and angiographic outcomes of the treatment of the huge dissecting aneurysms involving the BA by the internal trapping （IT） technique. Methods： We retrospectively studied 15 patients with the huge dissecting aneurysms involving the BA treated by the IT technique between September 2005 and September 2014 in Department of Interventional Neuroradiology of Beijing Tiantan Hospital. Clinical and angiographic data were reviewed and evaluated. Results： All patients were treated by the IT technique. That meant the dissecting artery and aneurysm segments were completed occlusion. After the procedure, the angiography demonstrated that all the dissecting artery and aneurysm segments were completed occlusion. Follow-up angiography was performed at 3-6 months or 12-18 months alter the endovascular treatment （median 8 months）, 14 patients had a good recovery. Re-canalization occurred in one patient whose aneurysm involved in bilateral vertebral arteries and the two third of the middle-lower BA. After the second treatment, the patient died by the ventricular tachycardia. Conclusions： The IT technique is a technically feasible and safe alternative for the treatment of BA dissecting aneurysms, but it is not necessarily the safest or most definitive treatment modality. The ideal treatment of the huge dissecting aneurysms involving the BA remains debatable and must be investigated on a case-by-case basis.

关键词： Basilar Artery Endovascular Treatment Huge Dissecting Aneurysms Internal Trapping Technique

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黏菌素耐药肺炎克雷伯菌耐药性及同源性

中华医院感染学杂志 2024年第1期34卷 1-6页

作者：萧晨路韩立中杨之涛徐玉敏倪语星石大可上海交通大学医学院附属瑞金医院临床微生物科上海200025 上海交通大学医学院附属瑞金医院急诊科上海200025 上海交通大学医学院附属瑞金医院医院感染管理科上海200025

目的了解黏菌素耐药的肺炎克雷伯菌耐药特点、临床分布、耐药性表型及基因型,并对菌株进行同源性分析。方法收集2021年1月-2021年12月上海交通大学医学院附属瑞金医院临床分离的黏菌素耐药肺炎克雷伯菌共17株,使用微生物质谱检测系统(MO... 详细信息

目的了解黏菌素耐药的肺炎克雷伯菌耐药特点、临床分布、耐药性表型及基因型,并对菌株进行同源性分析。方法收集2021年1月-2021年12月上海交通大学医学院附属瑞金医院临床分离的黏菌素耐药肺炎克雷伯菌共17株,使用微生物质谱检测系统(MOLDI-TOF MS)进行菌株鉴定,Vitek-2 Compact全自动微生物分析仪进行药敏试验,肉汤稀释法确认黏菌素敏感性,对菌株携带的耐药基因型进行PCR扩增和序列分析,分析菌株之间的同源性。结果17株黏菌素耐药的肺炎克雷伯菌菌株中,血液中分离率最高(4株占23.5%),科室分布以ICU为主(11株占64.7%);黏菌素耐药的肺炎克雷伯菌对β-内酰胺酶抑制剂复合制剂、头孢吡肟、环丙沙星、左氧氟沙星耐药率达100%;均携带bla_(SHV)和bla_(TEM),未检出mcr基因;其中16株(94.1%)多位点序列分型(MLST)为ST-11型;17例分离出黏菌素耐药肺炎克雷伯菌患者均合并多种基础疾病,13例(76.5%)为男性,年龄(37~96)岁,住院天数(33~146)天,9例(52.9%)死亡,4例(23.5%)检出前未接受任何黏菌素相关治疗;系统进化树显示不同部位分离的15株黏菌素耐药肺炎克雷伯菌在进化关系上具有同源性。结论黏菌素耐药肺炎克雷伯菌为广泛耐药菌株,大多携带多种耐药基因,治疗难度大,需引起临床重视,加强耐药菌株防控,合理选用抗菌药物。

关键词：黏菌素耐药肺炎克雷伯菌基因组学耐药基因同源性

Differential diagnosis and laparoscopic treatment of adrenal pheochromocytoma and ganglioneuroma

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Chinese Medical Journal 2009年第15期122卷 1790-1793页

作者： SHI Bing-bing li han-zhong CHEN Cheng RONG Shi FAN Hua WEN Jin li Hong-jun Department of Urology Peking Union Medical College Hospital Chinese Academy of Medical Sciences Beijing 100730 China

Background Adrenal ganglioneuroma is a rare adrenal pathogenic disease with difficult differential diagnosis from adrenal pheochromocytoma. Currently, very limited literature is available to allow a differential diagnosis of these two conditions from each other. This study aimed to evaluate the clinical profile, differential diagnosis and surgical treatments of both conditions. Methods Clinical characteristics of 36 patients with adrenal pheocheomocytoma and 18 patients with adrenal ganglioneuroma were analyzed. Data from CT scans and surgical treatments from 1999 to 2007 were collected. Endocrine hormone tests and ^131I-metaiodobenzylguanidine （MIBG） were performed. Neither ^131I-MIBG nor endocrine hormone tests were available in 9 cases of asymptomatic adrenal ganglioneuroma with tumor size less than 4 cm and there were negative findings from contrast enhanced CT scans. The level of urine catecholamine of patients was compared by one-way analysis of variance. Results The mean age of patients in the adrenal ganglioneuroma group was 41.2 years （16-67 year） and in the adrenal pheochromocytoma patients 38 years （17-74 year）. Contrast enhanced CT showed that the foci were intensified in 5 cases （27.8%） of adrenal ganglioneuroma and there were obvious contrast indications in 30 （83.3%） of the pheochromocytoma. Catecholamine levels in a 24-hour urine sample were normal in 4 patients with adrenal ganglioneuroma and increased in 36 （100%） cases with adrenal pheochromocytoma. ^131I-MIBG nuclear scan showed negative results in 4 patients （100%） with adrenal ganglioneuroma and positive results in 25 （96.2%） with adrenal pheochromocytom. Laparoscopy for adrenal tumors was performed through a transperitoneal or retroperitoneal approach during a follow-up period of （43±6） months, and all cases survived well. Conclusions CT, urinary catecholamine and ^131I-MIBG are standard and efficient tools for differential diagnosis of adrenal ganglioneuroma from pheochromocytoma. Laparoscopi

关键词： pheochromocytoma ganglioneuroma, laparoscopy

Effect of Mo and cold forging deformation on strength and ductility of cobalt-based alloy L605

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Journal of Iron and Steel Research International 2024年第7期31卷 1811-1823页

作者： zhong-lin Wang Zhi-hua Gong Quan li han-sheng Bao School of Materials and Metallurgy Inner Mongolia University of Science and TechnologyBaotou 014017Inner MongoliaChina Institute of Special Steels Central Iron and Steel Research Institute Co.Ltd.Beijing 100081China

Mo element was added to cobalt-based alloy L605,and cold forging deformation was *** effects of the addition and cold forging deformation on the microstructure and mechanical properties of the alloy were studied by thermodynamic calculation,electron backscatter diffraction,transmission electron microscopy,and X-ray *** stacking fault energy(SFE)of the alloy decreased after the addition,and the formation of stacking faults and intersections were promoted to improve the strength and *** tensile strength of the alloy with Mo increased from 1190 to 1702 MPa after 24%cold deformation,producing significant work *** strengthening mechanism is strain-induced martensitic transformation(SIMT)and deformation *** alloy,combined with Mo and after 24%deformation,had both high strength and ductility in comparison with the original cobalt-based alloy *** is attributed to the lower SFE which caused the increase in stacking fault *** the tensile process,theε-hcp phase was easily generated at the stacking fault to reduce the stress concentration and increase the *** SIMT by adjusting the density of stacking faults can improve the mechanical properties of cobalt-based ***ε-hcp phase,the interaction between deformation twins and dislocations,and the interaction between e-hcp phases during cold forging deformation caused local stress concentration,lowering ductility and toughness.

关键词： Cobalt-based alloy-Stacking fault energy Strain-induced martensitic transformation Deformation twin Cold forging deformation

Research on Gait Trajectory Planning of Wall-Climbing Robot Based on Improved PSO Algorithm

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Journal of Bionic Engineering 2024年第4期21卷 1747-1760页

作者： Jian li Xianlin Shi Peng liang Yanjun li Yilin Lv Mingyue zhong Zezhong han School of Mechanical and Automotive Engineering Guangxi University of Science and TechnologyLiuzhou545006China

In order to reduce the labor intensity of high-altitude workers and realize the cleaning and maintenance of high-rise building exteriors,this paper proposes a design for a 4-DOF bipedal wall-climbing bionic robot inspired by the inchworm’s *** robot utilizes vacuum adsorption for vertical wall attachment and legged movement for *** enhance the robot’s movement efficiency and reduce wear on the adsorption device,a gait mimicking an inchworm’s movement is planned,and foot trajectory planning is performed using a quintic polynomial *** velocity constraints,foot trajectory optimization is achieved using an improved Particle Swarm Optimization(PSO)algorithm,determining the quintic polynomial function with the best fitness through ***,through comparative experiments,the climbing time of the robot closely matches the simulation results,validating the trajectory planning method’s accuracy.

关键词： Bionic robot Wall-climbing robot PSO Gait planning Trajectory planning

Piggyback liver transplant techniques in the surgical management of urological tumors with inferior vena cava tumor thrombus

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Chinese Medical Journal 2009年第18期122卷 2155-2158页

作者： JI Zhi-gang XUE Chong li han-zhong WANG Hui-jun XIE Yi liU Guan-hua Department of Urology Peking Union Medical College Hospital Chinese Academy of Medical Sciences Beijing 100730 China

Background An important characteristic of renal cell carcinomas and adrenal tumors is that these tumors may expand into the renal vein and inferior vena cava, and transform into tumor thrombi. This study was to evaluate the use of piggyback liver transplant techniques for surgical management of urological tumors with inferior vena cava tumor thrombus. Methods Nineteen patients with renal cell carcinomas or adrenal tumors with inferior vena cava tumor thrombus were treated from November 1995 to April 2008. Their ages ranged from 29 years to 76 years （mean 54 years）. The extent of tumor thrombus was infrahepatic （level Ⅰ） in 2, retrohepatic （level Ⅱ） in 7, suprahepatic （level Ⅲ） in 6, and intra-atrial （level Ⅳ） in 4 patients. We used cardiopulmonary bypass with deep hypothermic circulatory arrest to remove the thrombi in 3 cases of level IV and in 2 cases of level Ⅲ. In all level Ⅱ, 4 level Ⅲ, and 2 level IV cases, we used piggyback liver transplant techniques to mobilize the liver off of the inferior vena cava and to separate the inferior vena cava from the posterior abdominal wall. Results Mean operative time was 5.1 hours, mean estimated blood loss was 2289 ml and mean blood transfusion was 12.84 U. One patient with adrenal cortical carcinoma and level Ⅳ thrombus died in the immediate postoperative period. Three patients were lost to follow up, and the other 15 survivors were followed from 5 months to 56 months. Eight of these 15 patients died due to metastasis; however 7 were still alive at the last follow-up. Conclusions An aggressive surgical approach is the only hope for curing patients diagnosed with urological tumors combined with inferior vena cava tumor thrombus. The use of piggyback liver transplant techniques to mobilize the liver off of the inferior vena cava provides excellent exposure of the inferior vena cava. Patients with a level Ⅱ or level Ⅲ inferior vena cava thrombus may be treated without using cardiopulmonary bypass.

关键词： renal cell carcinoma tumor thrombus inferior vena cava liver transplantation